scholarly journals Review of New Trends in the Analysis of Allergenic Residues in Foods and Cosmetic Products

2020 ◽  
Vol 103 (4) ◽  
pp. 997-1028 ◽  
Author(s):  
Tomasz Tuzimski ◽  
Anna Petruczynik
Keyword(s):  
Posttranslational Modifications ◽  
Allergic Diseases ◽  
Immunological Methods ◽  
Quantitative Detection ◽  
Screening Methods ◽  
Industrialized Countries ◽  
Allergen Sensitization ◽  
Important Health ◽  
Recent Developments ◽  
Allergen Analysis

Abstract Background Allergies represent an important health problem in industrialized countries. Allergen sensitization is an important risk factor for the development of allergic diseases; thus, the identification of an individual’s allergen sensitization is essential for the diagnosis and treatment of diseases. Objective This review compares different modern methods applied for the analysis of allergens in various matrices (from 2015 to the end of September 2019). Conclusions Immunological methods are still most frequently used for detection of allergens. These methods are sensitive, but the lack of specificity and cross-reaction of some antibodies can still be a relevant source of errors. DNA-based methods are fast and reliable for determination of protein allergens, but the epitopes of protein allergens with posttranslational modifications and their changes, originated during various processing, cannot be identified through the use of this method. Methods based on application of biosensors are very rapid and easy to use, and can be readily implemented as screening methods to monitor allergens. Recent developments of new high-resolution MS instruments are encouraging and enable development in the analysis of allergens. Fast, very sensitive, reliable, and accurate detection and quantification of allergens in complex samples can be used in the near future. Mass spectrometry coupled with LC, GC, or electrophoretic methods bring additional advances in allergen analysis. The use of LC-MS or LC-MS/MS for the quantitative detection of allergens in various matrices is at present gaining acceptance as a protein-based confirmatory technique over the routinely performed enzyme-linked immunosorbent assays.

scholarly journals Impact of diet on the immunological microenvironment of the pregnant uterus and its relationship to allergic disease in the offspring: a review of the recent literature

2006 ◽  
Vol 124 (5) ◽  
pp. 298-303 ◽  
Author(s):  
Daniella Campelo Batalha Cox Moore ◽  
Pedro Xavier Elsas ◽  
Elisabeth Santos Maximiano ◽  
Maria Ignez Capella Gaspar Elsas
Keyword(s):  
Allergic Disease ◽  
Allergic Diseases ◽  
Perinatal Period ◽  
Time Interval ◽  
Industrialized Countries ◽  
Pregnant Uterus ◽  
Medical Progress ◽  
Immunological Profile ◽  
Dietary Content ◽  
The Relationship

Medical progress has reduced the mortality from infectious diseases in most countries, but allergic diseases have become more prevalent worldwide over the same period, especially in industrialized countries. This has prompted speculation that modern lifestyles have altered the relationship between heredity and environment so as to promote development of an atopic phenotype when exposure to infection decreases. A healthy uterine microenvironment is known to favor Th2 lymphocyte development. However, some evidence suggests that persistence of the Th2 pattern of immunity directs the developing organism's immune response towards a long-lasting atopic phenotype. Even though the outcome also depends on other factors (such as infection, functional state of the intestinal microflora, and exposure to environmental allergens at times critical to development), it seems that the immune system during the perinatal period is responsive to interventions that are no longer effective in adulthood. We have reviewed the literature accessible through Medline to identify recent advances in the prevention of allergic disease through interventions in the fetal-maternal relationship. Diet seems to have a significant impact on the immunological profile of the pregnant uterus, as well as on the postnatal development of allergic disease in the offspring, as suggested by the effects of probiotic bacteria and by manipulations of the dietary content of polyunsaturated fatty acids and antioxidants. This highlights the need for further studies, in order to define the best intervention methods, the most appropriate time interval and the individuals who will most likely benefit from them.

Host defense in malnutrition

PEDIATRICS ◽  
1977 ◽  
Vol 59 (4) ◽  
pp. 490-495
Author(s):  
Michael Katz ◽  
E. Richard Stiehm
Keyword(s):  
Immune Response ◽  
Model Systems ◽  
Major Surgery ◽  
Nutrient Deficiencies ◽  
Industrialized Countries ◽  
Critical Approach ◽  
Value Judgment ◽  
Nutritional Interventions ◽  
A Value ◽  
Important Health

It has not been possible to relate a particular immune failure or deficiency to absence of a specific nutrient. All of these studies have viewed the totality of malnutrition and, yet, it would seem reasonable to expect that specific nutrient deficiencies would affect the response differently and that the almost infinite number of possible interactions among nutrients may have their particular effect. Furthermore, it has not been possible to evaluate any of these studies in terms of the influences that infections themselves have brought to bear. These considerations have led us to conclude that studies of man cannot be carried out with a precision required for the definitive answers. This is not a defeatist notion, but rather a value judgment that prompts us to recommend a more intensive series of investigations in animal model systems, the conclusions of which could then be applied selectively to clinical studies of man. The important question that must be considered is possible nutritional interventions in severely infected patients, such as those undergoing major surgery or immunosuppressive therapy. Potential dangers of using live attenuated viral vaccines in malnourished populations must be evaluated. It is necessary to assess the possibility that such vaccine viruses unchecked by normal immune response in malnourished individuals may lead to the establishment of states of latency and, ultimately, slow infections. The need for further studies has been given recognition by a workshop conference held in May 1975 under the title "Malnutrition and the Immune Response"; the proceedings are soon to be published in book form.57 The question of the influences of infection upon the state of nutrition has also been considered in a recent workshop, the proceedings of which will also be published.58 We believe that the current critical approach to this important health problem will generate answers long wanting. When these answers do become available, they will guide us into better care not only of the malnourished in the developing world, but also of those in the industrialized countries.

Mass Spectrometry-Based Determination of Thyroid Hormones and Their Metabolites in Endocrine Diagnostics and Biomedical Research – Implications for Human Serum Diagnostics

2020 ◽  
Vol 128 (06/07) ◽  
pp. 358-374 ◽  
Author(s):  
Josef Köhrle ◽  
Keith H. Richards
Keyword(s):  
Wide Spectrum ◽  
Quantitative Detection ◽  
Matrix Interferences ◽  
Novel Technology ◽  
Sample Extraction ◽  
Recent Developments ◽  
Serum Diagnostics ◽  
Novel Applications ◽  
Total Concentrations

AbstractThe wide spectrum of novel applications for the LC-MS/MS-based analysis of thyroid hormone metabolites (THM) in blood samples and other biological specimen highlights the perspectives of this novel technology. However, thorough development of pre-analytical sample workup and careful validation of both pre-analytics and LC-MS/MS analytics, is needed, to allow for quantitative detection of the thyronome, which spans a broad concentration range in these biological samples.This minireview summarizes recent developments in advancing LC-MS/MS-based analytics and measurement of total concentrations of THM in blood specimen of humans, methods in part further refined in the context of previous achievements analyzing samples derived from cell-culture or tissues. Challenges and solutions to tackle efficient pre-analytic sample extraction and elimination of matrix interferences are compared. Options for automatization of pre-analytic sample-preparation and comprehensive coverage of the wide thyronome concentration range are presented. Conventional immunoassay versus LC-MS/MS-based determination of total and free THM concentrations are briefly compared.

scholarly journals Recent developments and highlights in biomarkers in allergic diseases and asthma

Allergy ◽  
2018 ◽  
Vol 73 (12) ◽  
pp. 2290-2305 ◽  
Author(s):  
Ibon Eguiluz-Gracia ◽  
Tunn Ren Tay ◽  
Mark Hew ◽  
Maria M. Escribese ◽  
Domingo Barber ◽  
...  
Keyword(s):  
Allergic Diseases ◽  
Recent Developments

scholarly journals Mutagenesis as a Tool in Plant Genetics, Functional Genomics, and Breeding

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Per Sikora ◽  
Aakash Chawade ◽  
Mikael Larsson ◽  
Johanna Olsson ◽  
Olof Olsson
Keyword(s):  
High Resolution ◽  
Molecular Breeding ◽  
Molecular Level ◽  
Coming Of Age ◽  
Screening Methods ◽  
Comprehensive Overview ◽  
Local Lesions ◽  
Recent Developments ◽  
Genetic Targeting ◽  
Traditional Breeding

Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. By combining the high variation of mutagenised populations with novel screening methods, traits that are almost impossible to identify by conventional breeding are now being developed and characterised at the molecular level. This paper provides a comprehensive overview of the various techniques and workflows available to researchers today in the field of molecular breeding, and how these tools complement the ones already used in traditional breeding. Both genetic (Targeting Induced Local Lesions in Genomes; TILLING) and phenotypic screens are evaluated. Finally, different ways of bridging the gap between genotype and phenotype are discussed.

scholarly journals The Association Between Intestinal Bacteria and Allergic Diseases—Cause or Consequence?

2021 ◽  
Vol 11 ◽  
Author(s):  
Pei Han ◽  
Jian-Qing Gu ◽  
Li-Sha Li ◽  
Xue-Yan Wang ◽  
Hong-Tian Wang ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Intestinal Bacteria ◽  
Allergic Diseases ◽  
Therapeutic Interventions ◽  
Disease Development ◽  
Industrialized Countries ◽  
The Past ◽  
Microbial Analysis ◽  
Preclinical And Clinical Studies ◽  
Allergic Disorders

The incidence of allergic disorders has been increasing over the past few decades, especially in industrialized countries. Allergies can affect people of any age. The pathogenesis of allergic diseases is complex and involves genetic, epigenetic, and environmental factors, and the response to medication is very variable. For some patients, avoidance is the sole effective therapy, and only when the triggers are identifiable. In recent years, the intestinal microbiota has emerged as a significant contributor to the development of allergic diseases. However, the precise mechanisms related to the effects of the microbiome on the pathogenesis of allergic diseases are unknown. This review summarizes the recent association between allergic disorders and intestinal bacterial dysbiosis, describes the function of gut microbes in allergic disease development from both preclinical and clinical studies, discusses the factors that influence gut microbial diversity and advanced techniques used in microbial analysis. Ultimately, more studies are required to define the host-microbial relationship relevant to allergic disorders and amenable to new therapeutic interventions.

scholarly journals PD-L1 Blockade During Allergen Sensitization Inhibits the Synthesis of Specific Antibodies and Decreases Mast Cell Activation in a Murine Model of Active Cutaneous Anaphylaxis

2021 ◽  
Vol 12 ◽  
Author(s):  
Rafael Bonamichi-Santos ◽  
Marcelo Vivolo Aun ◽  
Jorge Kalil ◽  
Mariana Concepcion Castells ◽  
Pedro Giavina-Bianchi
Keyword(s):  
Mast Cell ◽  
Murine Model ◽  
Cell Activation ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Mast Cell Activation ◽  
Allergen Sensitization

Programmed cell death ligand 1(PDL-1) is known for its inhibitory effect on the cellular immune response. Even though it is expressed on the surface of mast cells, its role in allergic diseases is unknown. We analyzed the effects of PD-L1 blockade in a murine model of active cutaneous anaphylaxis (ACA). C57BL/6 mice were sensitized and challenged with ovalbumin (OVA). Blood samples were collected to measure specific immunoglobulins. The mice were divided into six groups that underwent the active cutaneous anaphylaxis procedure. Group 1 (negative control) received 50 μl of phosphate-buffered saline (PBS) subcutaneously, and the other five groups were sensitized with 50 μg of OVA subcutaneously. Group 2 was the positive control, and the others received the anti-PD-L1 antibody or its isotype during sensitization (groups 3 and 4) or during the challenge (groups 5 and 6). All animals that underwent ACA on the ears with OVA and PBS were sacrificed, and the reaction was evaluated by extravasation of Evans blue (measured by spectrophotometry) and histological analysis of the collected fragments. Anti-PD-L1 blockade during the sensitization phase led to a reduction in specific IgE and IgG1 levels, allergic reaction intensity at the ACA site, and mast cell degranulation in the tissue. There was no significant biological effect of anti-PD-L1 administration on the challenge phase. PD-L1 blockade during allergen sensitization inhibited the synthesis of specific IgE and IgG1 and decreased mast cell activation in this murine model of anaphylaxis.

scholarly journals Global financial crisis

2016 ◽  
Vol 1 (1) ◽  
pp. 67
Author(s):  
MSc. Jusuf Qarkaxhija ◽  
MSc. Labinot Hashani
Keyword(s):  
Financial Crisis ◽  
Global Financial Crisis ◽  
Stock Exchange ◽  
High Rate ◽  
Economic Policies ◽  
Industrialized Countries ◽  
Recent Developments ◽  
Global Aspect ◽  
Countries In Transition ◽  
Political Economic

The most recent developments in economy are a clear indicator of many changes, which are a result of this high rate pacing, which also demonstrates as such. Market economy processes occur as a result of intertwining of many potential technological and human factors, thereby creating a system of numerous diver-gences and turbulences. Economics, a social science, is characteri-sed with movements from a system to another system, and is har-monized with elements or components which have impacted the development and application of economic policies as a result. This example can be illustrated with the passing from a commanded system (centralized) to a self-governing (decentrali-zed) system, while the movement from a system to another is known as transi-tion. Such transition in its own nature bears a number of problems of almost any kind (political, economic, social, etc.), and is charac-terised with differences from a country to another.Financial crisis is a phenomenon consisting of a perception of economic policies and creation of an economic and financial stabi-lity in regional and global structures. From this, one may assume that each system has its own changes in its nature, and as a result of these changes, we have the crisis of such a system. Even in the economic field, if we look closely, we have such a problem, where development trends both in human and technological fields have created a large gap between older times and today, thereby crea-ting dynamics with a high intensity of action. If we dwell on the problem, and enter into the financial world, we can see that the so-called industrialized countries have made giant leaps in deve-lopment, while countries in transition have stalled in many fields, as a result of a high rate of corruption and unemployment in these countries, and obviously these indicators are directly connected, thereby stroking the financial system in these countries.Corruption is an element, which directly and indirectly influences the pro-cess of attracting foreign investment, and further influencing the growth of unemployment, and in turn expanding the financial crisis, where finances are already fragile.In the following sections, we will elaborate on the financial crisis in a global aspect, the impacts of this crisis in economic development, and the role of stock exchange in finance, thereby creating a multi-dimensional horizon of the problem.

scholarly journals Current Perspectives in Human Papilloma Virus: Where We are and What We Need?

2019 ◽  
Vol 12 (04) ◽  
pp. 1683-1700
Author(s):  
B.S Anjali Devi ◽  
Venugopal Reddy Bovilla ◽  
SubbaRao V. Madhunapantula
Keyword(s):  
Diagnostic Methods ◽  
The Other ◽  
Screening Methods ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Sequencing Technologies ◽  
Public Health Screening ◽  
Highly Sensitive ◽  
Recent Developments ◽  
Hpv 18

It is now well-established that Human Papilloma Viruses (HPV) are responsible for causing >90% of cervical cancers. Existing evidences have also demonstrated a key role for HPV in a portion of Head and Neck cancers as well as carcinomas of Vulva, Vagina, Penis and Anus. Therefore studies aiming at developing highly sensitive diagnostic methods have become high-priority in the recent years. To date, an estimated 202 types of HPV have been identified, of which only a small percentage viruses are involved in carcinogenesis. Among the carcinogenic HPV, the most predominant ones are HPV 16 and HPV 18. The other high risk types are 31, 33, 34, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, and 70. In general, HPV is detected by polymerase chain reaction (PCR) and hybrid capture (HC) methods. However, the existing PCR methods specifically identify only HPV 16 and HPV 18, but not the other types. More over, the PCR protocols are known for errors, and are not suitable for on-field screening procedures, hence, a suitable much quicker and sensitive method is required at the earliest. More over, the PCR and HC methods are expensive, hence, require more funds to conduct public health screening campaigns. Therefore, in this book chapter we have made an attempt to address the recent developments in HPV screening methods, and covered various advanced procedures available in the literature to identify HPV in patient specimens. For example, a section is dedicated to cover the “Biosensors”, which have been developed in detecting the HPV DNA in body fluids. These devices are much simpler compared to sequencing technologies and likely to be the next-generation detection devices for HPV

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