scholarly journals 24 Determining the phosphorus release of Smizyme TS G5 2,500 phytase in nursery pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 39-39
Author(s):  
Madie R Wensley ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
Mike D Tokach ◽  
Robert D Goodband ◽  
...  

Abstract Two nursery trials (DNA 241×600) with 320 or 280 pigs, respectively (initially 10 kg) were used to determine the available P (aP) release curve for Smizyme TS G5 2,500 (Origination, Inc., Saint Paul, MN). Pigs were weaned at approximately 21-d of age, randomly allotted to pens based on initial body weight (BW) and fed a common diet. On d 21 post-weaning, pens were blocked by BW and randomly allotted to 1 of 8 (Exp. 1) or 7 (Exp. 2) dietary treatments with 5 pigs/pen and 8 pens/treatment. Treatments were formulated to include increasing aP from either inorganic P (0.12%, 0.18%, or 0.24% in Exp. 1 and 0.11%, 0.19%, or 0.27% in Exp. 2 from monocalcium P) or increasing phytase (150, 250, 500, 750, or 1,000 FTU/kg in Exp. 1 and 250, 500, 1000, or 1,500 FTU/kg in Exp. 2). Prior to beginning the 21-d studies, all pigs were fed the lowest inorganic P diet for a 3-d period. At the conclusion of the experiments, the pig closest to the pen mean BW was euthanized and the right fibula was collected to determine bone ash. In both experiments, pigs fed increasing aP from inorganic P had increased (linear, P< 0.01) ADG, G:F, and final BW. Additionally, pigs fed diets with increasing phytase had increased (Exp. 1 linear, P< 0.01, Exp. 2 linear and quadratic, P< 0.05) ADG, ADFI, and G:F. The aP release increased for ADG (Exp. 1 linear, P< 0.01; Exp. 2 linear and quadratic, P< 0.01), G:F (linear, P< 0.01), and bone ash percent (Exp. 1 linear and quadratic, P< 0.05; Exp. 2 linear, P< 0.01) up to the highest phytase inclusion. When combining the release values from Exp. 1 and 2, the release equations for Smizyme TS G5 2,500 are aP=(0.197×FTU)÷(584.956+FTU), aP=(0.175×FTU)÷(248.348+FTU), and aP=(0.165×FTU)÷(178.146+FTU) for ADG, G:F, and bone ash percent, respectively.

Author(s):  
Larissa L Becker ◽  
Madie R Wensley ◽  
Joel M DeRouchey ◽  
Jason C Woodworth ◽  
Mike D Tokach ◽  
...  

Abstract The objective of this study was to determine the available P (aP) release curve for a new phytase source, GraINzyme Phytase (Agrivida Inc., Woburn, MA), which is expressed in corn containing an engineered Escherichia coli phytase called Phy02. Plant-expressed phytases are created by inserting phytase-encoding genes into plants resulting in their ability to produce seeds with increased concentrations of phytase. A total of 360 pigs (Line 200 × 400, DNA, Columbus, NE, initially 9.9 ± 0.19 kg) were used in a 21-d growth study. Pigs were weaned at approximately 21-d of age, randomly allotted to pens based on initial body weight (BW) and fed common starter diets. From d 18 to 21 post-weaning, all pigs were fed a diet containing 0.11% aP. On d 21 post-weaning, considered d 0 of the study, pens were blocked by BW and randomly allotted to 1 of 8 dietary treatments with 5 pigs per pen and 9 pens per treatment. Dietary treatments were formulated to include increasing aP derived from either an inorganic P source (0.11, 0.19, or 0.27% from monocalcium P) or increasing phytase (150, 250, 500, 1,000, or 1,500 FTU/kg). Diets were corn-soybean meal-based and contained 1.24% standardized ileal digestible (SID) Lys. On d 21 of the trial, 1 pig per pen (weighing closest to the mean pen BW) was euthanized and the right fibula was collected to determine bone ash using the non-defatted processing method. Overall (d 0 to 21), pigs fed increasing aP from inorganic P or phytase had increased (linear, P < 0.002) ADG, ADFI, and G:F (quadratic, P < 0.05). Bone ash weight (g) and percentage bone ash increased (linear, P < 0.001) with increasing inorganic P or added phytase. Based on the composition of the diets used in this study, the release equations developed for GraINzyme for ADG, G:F, bone ash weight, and percentage bone ash are: aP = (0.255 × FTU) ÷ (1299.969 + FTU), aP = (0.233 × FTU) ÷ (1236.428 + FTU), aP = (45999.949 × FTU) ÷ (462529200 + FTU), and aP = (0.272 × FTU) ÷ (2576.581 + FTU), respectively.


2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Madie R Wensley ◽  
Joel M DeRouchey ◽  
Jason C Woodworth ◽  
Mike D Tokach ◽  
Robert D Goodband ◽  
...  

Abstract Two experiments were conducted to determine the available P (aP) release of Smizyme TS G5 2,500 (Origination, LLC., Maplewood, MN) phytase. Pigs were weaned at approximately 21-d of age, randomly allotted to pens based on initial body weight (BW) and fed a common diet. On d 21 post-weaning, pens were blocked by BW and randomly allotted to 1 of 8 (experiment 1) or 7 (experiment 2) dietary treatments with five pigs per pen and eight pens per treatment. Treatments were formulated to include increasing aP from either inorganic P (0.12%, 0.18%, or 0.24% in experiment 1 and 0.11%, 0.19%, or 0.27% in experiment 2 from monocalcium P) or increasing phytase (150, 250, 500, 750, or 1,000 FTU/kg in experiment 1 and 250, 500, 1,000, or 1,500 FTU/kg in experiment 2). Prior to beginning the 21-d studies, all pigs were fed the lowest inorganic P diet for a 3-d period. At the conclusion of each experiment, the pig closest to the pen mean BW was euthanized and fibulas were collected to determine bone ash weight and percentage bone ash. Fibulas were processed using defatted bone mineral procedures. In both experiments, pigs fed increasing aP from inorganic P had increased (linear, P < 0.01) average daily gain (ADG), G:F, and final BW. Additionally, pigs fed diets with increasing phytase had increased (experiment 1 linear, P < 0.01; experiment 2 linear and quadratic, P < 0.05) performance across all growth response criteria. For bone composition, pigs fed increasing aP from inorganic P had increased bone ash weights (linear, P < 0.01) and percentage bone ash (experiment 1 quadratic, P < 0.01; experiment 2 linear, P < 0.01). Similarly, pigs fed increasing phytase had increased bone ash weights (linear, P < 0.01) and percentage bone ash (experiment 1 linear, P < 0.01; experiment 2 linear and quadratic, P < 0.05). The percentage aP released from Smizyme TS G5 2,500 for both experiments varied depending on the response criteria used. As the amount of phytase in the diet increased, the calculated aP release increased when ADG (experiment 1 linear, P < 0.01; experiment 2 linear and quadratic, P < 0.01), G:F (linear, P < 0.01), or percentage bone ash (experiment 1 linear and quadratic, P < 0.05; experiment 2 linear, P < 0.01) were used the predictor variable. When combining the data from experiment 1 and 2, the aP release prediction equations for Smizyme TS G5 2,500 are aP = (0.197 × FTU)/(584.956 + FTU), aP = (0.175 × FTU)/(248.348 + FTU), and aP = (0.165 × FTU)/(178.146 + FTU) when using ADG, G:F, and percentage bone ash, respectively as the predictor variable.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 212-213
Author(s):  
Ping Ren ◽  
Joe Cushing ◽  
Karen Wedekind ◽  
Mercedes Vazquez-Anon

Abstract This study investigated the interactive effects of Zn sources, Cu sources and phytase on growth performance in nursery pigs. A total of 288 weaning barrows (BW = 5.71 ± 0.81 kg), blocked by initial body weight, were randomly allotted to 1 of 8 dietary treatments, with 9 pens per treatment and 4 pigs per pen. The 8 dietary treatments were arranged in 2 × 2 × 2 factorial design, with 2 Zn sources [2000 ppm Zn from ZnO during phase 1 (d 1 to 14) and 2 (d 15 to 28), and 100 ppm Zn from ZnO during phase 3 (d 29 to 42); 100 ppm Zn from Zn methionine hydroxy analogue chelate (Zn-MHAC, MINTREX® Zn, Novus International Inc., St. Charles, MO) during phase 1 to 3], 2 Cu sources [150 ppm Cu from CuSO4 or Cu-MHAC (MINTREX® Cu) during phase 1, and 80 ppm Cu from CuSO4 or Cu-MHAC during phase 2 and 3], and 2 phytase inclusion levels (0, 500 FTU/kg). Results showed that there were no differences (P > 0.10) between ZnO and Zn-MHAC groups in terms of growth performance during the entire nursery period. Compared with CuSO4, Cu-MHAC tended to increase ADG (P = 0.07) and G:F (P = 0.08) during the entire nursery period. There tended to be an interaction (P = 0.09) between Cu sources and phytase on G:F during phase 2. Specifically, without phytase supplementation, there was no difference (P = 0.63) on G:F during phase 2 between Cu-MHAC and CuSO4, whereas with phytase supplementation, Cu-MHAC increased G:F during phase 2 (P = 0.05) compared with CuSO4. In conclusion, Zn-MHAC supplementation at 100 ppm could replace pharmacological level of ZnO without affecting growth performance. Cu-MHAC could improve growth performance compared with CuSO4. Cu-MHAC exerted synergistic effect with phytase on improving feed efficiency.


Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 174 ◽  
Author(s):  
Jung Yeol Sung ◽  
Beob Gyun Kim

The objectives were to determine effects of a hatchery byproduct mixture (HBM) on growth performance and to measure digestible energy concentrations in various HBM. In the growth performance experiment, 96 pigs (initial body weight = 9.6 ± 0.8 kg) were assigned to 4 dietary treatments in a randomized complete block design with 6 blocks. Each treatment consisted of 6 replicate pens with 4 pigs comprising 2 barrows and 2 gilts. Pigs were fed graded concentrations of HBM at 0%, 3.33%, 6.67%, and 10.00% for 14 days. In the energy digestibility experiment, 10 barrows (initial body weight = 11.5 ± 0.4 kg) were employed to determine digestible energy in HBM. A basal diet based on corn and soybean meal and 4 additional diets containing 25% of 4 different HBM were prepared. The marker-to-marker method was employed for total collection and the experimental design was a replicated 5 × 4 Latins square design. Growth performance was not compromised as the inclusion rate of HBM increased up to 10%. Digestible energy of HBM ranged from 2772 to 3887 kcal/kg as-is basis. In conclusion, HBM can be used in nursery pig diets and different energy values should be used for each HBM.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 417-417
Author(s):  
David M Crawford ◽  
Joshua C McCann

Abstract The objective was to determine the interaction of replacing forage with coproducts and the number of step-up diets when adapting feedlot cattle to a finishing diet on the ruminal microbiome. Simmental × Angus and Angus steers (n = 24; 234 ± 38 kg) were blocked by initial body weight (BW) and allotted to 8 pens as a subset of a larger group. Pens were randomly assigned to 1 of 2 dietary treatments: 1) decreasing coproduct (CO; soybean hulls and modified wet distillers grains) inclusion while increasing dry-rolled corn over 36 d or 2) decreasing forage (FO; alfalfa and grass hay) inclusion while increasing dry-rolled corn over 36 d. Pens were fed either 5 (5S) or 2 (2S) step-up diets for each dietary treatment during the 36 d adaptation period in a 2 × 2 factorial design. Steers were fed a common finishing diet for the remainder of the trial (34 d). The ruminal microbiome was evaluated on d 36 and 70. Alpha diversity was greatest (P < 0.01) for FO/2S steers on d 36. A diet × day effect (P = 0.02) was observed for beta diversity as the microbial community was more similar (P < 0.01) between d 36 and 70 for steers fed CO compared with FO. Relative abundance of Bacteroidetes was greater (P = 0.03) for 2S steers compared with 5S steers. Prevotellaceae tended to be greater (P = 0.07) in 2S steers compared with 5S steers. Lactobacillaceae was greater (P < 0.01) on d 36 than d 70. Limited observed day effects for individual taxa suggest the microbiome was well adapted by treatments for the finishing diet on d 36. Greater coproduct inclusion in adaptation diets may alter the ruminal bacterial community to be more similar to the community while feeding high levels of dry-rolled corn.


2018 ◽  
Vol 21 (1) ◽  
pp. 32
Author(s):  
SUMADI I K. ◽  
I P. ARI ASTAWA ◽  
A. A. P. P. WIBAWA ◽  
A. W. PUGER

This study was conducted to know the right amount of amino acid and choline (Aminovit) mixture in traditionalfeeds on male bali pigs for 12 weeks. The design used was a completely randomized design (CRD) with fourtreatments and each treatment consisted of 4 replications, so 16 male bali pigs using with a weight range of 11.60-14.10 kg. The feed treatment were P0 treatment: mixed base feed of 49.5% yellow maize and 49.5% pollard; P1:treatment P0 + 0,50% Aminovit; P2: P0 + 1.00% Aminovit; and P3: treatment P0 + 1.5 Aminovit. The performancemeasured were initial body weight, final body weight, body weight gain, feed intake, and feed conversion ratio(FCR). The results showed that increasing Aminovite in the feed increased of final body weight, body weight gainand feed consumption, and feed efficiency.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 90-90
Author(s):  
Jesus A Acosta ◽  
John F Patience

Abstract The objective of this experiment was to determine the effect of insoluble fiber on the efficacy of phytase in nursery pig diets. A total of 480 weaned pigs (BW 5.48 ± 0.14 kg; L337 X Camborough, PIC, Hendersonville, TN) were blocked by BW and randomly allotted to 48 pens. A common nutrient-adequate diet was fed from d 0–9. From d9-14, 2 phosphorus [P] deficient basal diets were fed: a low fiber [LF] corn-soybean meal diet (neutral detergent fiber [NDF]=7.5%; standardized total tract digestible [STTD] P = 0.16%) or a higher fiber [HF] corn-soybean meal plus 20% corn bran diet (NDF=15.2%; STTD P = 0.14%). From d 15–35, pigs received 8 dietary treatments (6 pens per treatment): 4 increasing levels of added phytase (0, 109, 218, and 327 phytase units [FTU]/kg; Quantum-Blue-5G, AB-Vista, Wiltshire, UK) applied to either the LF or HF basal diets. Titanium dioxide served as a digestibility marker. Fecal samples were collected on d 32–34. On d 35, one pig per pen was euthanized and fibulas harvested for bone ash assay. Data were analyzed using PROC MIXED of SAS. There were no interactions between fiber and phytase (P > 0.100). Adding phytase increased ADG (0.268, 0.282, 0.291, 0.306 kg/d; P < 0.001) and G:F (0.52, 0.55, 0.56, 0.58; P < 0.001) for the 4 increasing phytase levels, respectively. Fiber decreased ADG (0.282 vs 0.291 kg/d; P = 0.033) and tended to decrease G:F (0.54 vs 0.56; P = 0.097). There were no effects of phytase or fiber on ADFI (P > 0.100). Phytase increased bone ash (P = 0.005), but there was no effect of fiber (P = 0.949). Phytase did not affect the ATTD of DM, NDF, or ADF (P > 0.100) while fiber decreased the ATTD of DM, NDF and ADF (P < 0.001). In conclusion, increasing insoluble fiber did not affect the ability of phytase to improve growth performance and bone mineralization when pigs were fed a P deficient diet.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5791 ◽  
Author(s):  
Ah Reum Son ◽  
Jin-young Jeong ◽  
Kyu Ree Park ◽  
Minseok Kim ◽  
Sung Dae Lee ◽  
...  

The experiment was conducted to determine the effects of graded dietary selenium (Se) on organ weight and Se concentrations in tissues and to develop equations for estimating dietary Se intake in pigs. Sixteen barrows (initial body weight = 30.0 ± 2.6) were allotted to four dietary treatments including graded Se supplementations with 0, 1, 5, and 50 mg/kg of diet. The experimental diets fed to the pigs for 30 d, and then the pigs were euthanized, and the organs, muscle, and urine samples were collected. The hair and blood samples of pigs were collected on d 15 and 30. Equations were developed for predicting daily Se intake using the Se concentration in plasma, hair, liver, kidneys, muscle, or urine. For graded dietary Se concentrations, linear and quadratic effects on the final body weight, weight and relative weight of liver and kidneys were not observed. The Se concentration in plasma, hair, liver, kidneys, muscle, and urine were linearly and quadratically increased as dietary Se concentration increased (P < 0.001). The dietary Se concentration was positively correlated with the Se concentrations in the plasma, organs, muscle, and urine (r > 0.81,P < 0.001). The equations for estimating dietary Se intake using the Se concentration in the plasma, hair, or organ as an independent variable were significant (P < 0.05). In conclusion, the dietary Se concentration was well reflected in the Se concentration in the plasma, hair, liver, kidneys, and urine. The Se concentration in the plasma, hair, liver, and kidneys can be used as an independent variable for estimating the Se intake.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 353-354
Author(s):  
Deepak E Velayudhan ◽  
Leon Marchal ◽  
Yueming Dersjant-Li

Abstract A meta-analysis was conducted to investigate the effect of dietary calcium (Ca) levels and phytase dose on apparent total tract digestibility (ATTD) of phosphorus (P) in pigs fed corn or wheat-based diets. A database was constructed using 19 studies (published and unpublished), contributing 766 data points with 464 in nursery pigs and 303 in growing pigs. A Buttiauxella sp. phytase was added at 4 doses (250, 500, 1,000 and 2,000 FTU/kg) to P and Ca deficient negative control diets (NC) among different studies. Total analysed dietary Ca, total P and calculated phytate P levels varied from 0.41 to 0.97%, 0.36 to 0.67%, and 0.21 to 0.31%, respectively among studies. Data were analysed using Mixed Model by JMP 14.0. Since no significant differences were observed in ATTD P between nursery pigs (average initial body weight 7.5kg) and growing pigs (average initial body weight 40.0kg), the data were combined for the meta-analysis. Nonlinear modelling was used to evaluate the effect of dietary Ca on ATTD P and P digestibility improvement over the NC at different phytase levels. Increasing phytase dose from 0 to 2000 FTU/kg, improved (P &lt; 0.05) ATTD Ca and P in an exponential manner, with a greater ATTD P (P &lt; 0.05) over NC. With multivariate analysis, ATTD P had a negative correlation (P &lt; 0.05) with dietary Ca levels (-0.26), Ca:P ratio (-0.30) and dietary phytate content (-0.40). An interaction was found between Ca level and phytase dose for P digestibility improvement over NC, which was not significantly different at dose between 250 to 1000 FTU but increased (P &lt; 0.05) at phytase dose of 2000 FTU/kg with increasing dietary Ca. These findings suggest that supplementation of 2000 FTU phytase /kg could restore Ca and P balance and mitigate the negative effects of increasing dietary Ca on P digestibility.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 89-90
Author(s):  
Wade M Hutchens ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
Robert D Goodband ◽  
...  

Abstract A total of 1,215 pigs (L337×1050, PIC, Hendersonville, TN) were used in a 156-d wean-to-finish experiment. Pigs were weaned at 21 d of age and placed in pens based on initial body weight (BW) with 27 pigs/pen. There were three dietary treatments including a: 1) control; 2) pigs fed diets containing AviPlus (micro-encapsulated sorbic and citric acids and synthetic thymol and vanillin botanicals; Vetagro Inc., Chicago, IL) during the nursery and finisher phases; or 3) pigs fed AviPlus during the nursery but not the finishing phase. AviPlus was included at 2.72 kg/ton from d 0 to 21, 0.90 kg/ton from d 21 to 42, and 0.45 kg/ton from d 42 to 156. Thus, there were 15 control pens and 30 Aviplus pens in the nursery and 15 pens for the 3 treatments in the finisher phase. On d 42, pigs were transported as intact pens to the finishing facility. Data were analyzed as a RCBD with pen as experimental unit. For the overall nursery period (d 0 to 42), pigs fed AviPlus had improved (P &lt; 0.05) G:F, with no evidence of difference (P &gt; 0.05) for d 42 BW, ADG, or ADFI. For the overall finishing period (d 42 to 156) and overall experimental period (d 0 to 156), there was no evidence of difference (P &gt; 0.05) for BW, ADG, ADFI, or G:F. There was no evidence of difference (P &gt; 0.05) observed for mortality and removals during the nursery, finisher, or overall. In summary, providing AviPlus during the nursery improved nursery G:F, but there was no effect on overall wean-to-finish performance.


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