110 Evaluation of Macronutrient Composition and Amino Acid Digestibility of Select Novel Dietary Proteins for Use in Canine and Feline Nutrition

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 57-57
Author(s):  
Victoria A Gomez ◽  
Pam L Utterback ◽  
Carl M Parsons ◽  
Maria R de Godoy ◽  
Haley Russell
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dry Matter ◽  
Protein Quality ◽  
National Research Council ◽  
Nutrient Content ◽  
Balanced Diet ◽  
Amino Acid Score ◽  
Adult Cats ◽  
Limiting Amino Acids

Abstract Demand for the inclusion of high-quality protein in companion animal diets has increased as owners have become more invested in the health of their pets. There is very little information on the nutrient content of novel proteins needed for a properly balanced diet, so as a result the objectives of this study were to determine the chemical composition, standardized amino acid digestibility using the precision-fed cecectomized rooster assay, and protein quality using digestible indispensable amino acid score (DIAAS like) of raw chicken, spirulina, cricket meal, and fish (carp) slurry. Raw chicken has the lowest crude protein concentration (45.6%) on dry matter (DM) basis, whereas fish slurry had the highest (75.3%). All amino acids were moderately to highly digestible (70.2% to 98.8%, dry matter basis) with differences (P < 0.05) in all but arginine, phenylalanine, threonine, cysteine, serine, and tyrosine. The limiting amino acid based on DIAAS-like values for adult dogs compared with the Association of American Feed Control Officials (AAFCO) recommended values were tryptophan for raw chicken and methionine for cricket meal. Comparing DIAAS-like values for adult dogs to National Research Council (NRC) recommended allowances, the limiting amino acids were the same where raw chicken and fish slurry still had tryptophan and spirulina and cricket meal had methionine as the first limiting amino acids. The DIAAS-like values for adult cats compared to AAFCO and NRC values were all above 100% with the lowest being threonine for chicken (123.65%) compared to AAFCO nutrient profile for adult cats and histidine for spirulina (123.13%) compared to NRC for cats at maintenance. Based on the data gathered in this study, the novel dietary protein sources evaluated are of similar or superior protein quality compared with raw chicken; a traditional protein source widely used in pet foods.

scholarly journals Influence of macro and microelements on the biological value of wheat grain

2021 ◽  
Vol 937 (2) ◽  
pp. 022130
Author(s):  
V Isaychev ◽  
N Andreev ◽  
F Mudarisov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Spring Wheat ◽  
Protein Quality ◽  
Mineral Fertilizers ◽  
Control Group ◽  
Natural Fertility ◽  
Biological Value ◽  
Amino Acid Score ◽  
Limiting Amino Acids

Abstract This paper considers the influence of manganese and zinc sulfate, as well as Megamix-Zinc on the protein quality of grain of winter and spring wheat, when cultivating it on soils with a deficiency of certain microelements. It was found that treatment of seeds and growing plants of experimental crops with the studied preparations increased the content of the essential limiting amino acids in the grain of winter wheat - lysine by 85.7%, threonine by 116.7%, isoleucine + leucine by 20.9%, phenylalanine + tyrosine by 33.3% compared to the control variant. A rise in the amount and amino acid score of such limiting amino acids as lysine and threonine is also observed, which leads to a decline in the coefficient of difference of amino-acid scores up to 75.5% and an increase in the biological value of the total protein up to 24.5%. The total amount of amino acids in the grain of spring wheat increased when Megamix-Zinc was used in the cultivation technology, both on soils with natural fertility and with application of mineral fertilizers, which is associated with a rise in nitrogen and carbohydrate metabolism. The increase in relation to the control group is 0.42 - 1.34 mg/g, depending on the variant. Under the influence of the factors used, amino acid score of individual amino acids of the spring wheat protein increases in relation to the control group up to 2.1%.

scholarly journals Macronutrient composition, true metabolizable energy and amino acid digestibility, and indispensable amino acid scoring of pulse ingredients for use in canine and feline diets

2020 ◽  
Vol 98 (6) ◽  
Author(s):  
Lauren M Reilly ◽  
Patrick C von Schaumburg ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Pamela L Utterback ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dry Matter ◽  
Protein Quality ◽  
Metabolizable Energy ◽  
Macronutrient Composition ◽  
Total Dietary Fiber ◽  
Protein Sources ◽  
Indispensable Amino Acid ◽  
Limiting Amino Acid

Abstract The rising consumer demand for alternative and sustainable protein sources drives the popularity of the use of plant-based proteins in the pet food industry. Pulse crops, which include beans, peas, lentils, and chickpeas, have become an important addition to both human and animal diets due to their protein content and functional properties. However, knowledge of their nutrient composition and protein quality is necessary for the proper formulation of these ingredients in pet foods. The objective of this study was to determine the macronutrient composition and standardized amino acid digestibility and to describe the protein quality through the use of digestible indispensable amino acid scores (DIAAS-like) of five pulse ingredients. Black bean (BB) grits, garbanzo beans (GB), green lentils (GL), navy bean (NB) powder, and yellow peas (YP) were analyzed for dry matter (DM), ash and organic matter (OM), crude protein (CP), gross energy (GE), acid hydrolyzed fat (AHF), and total dietary fiber (TDF) to determine the macronutrient composition. Precision-fed rooster assays were conducted using cecectomized roosters to calculate standardized amino acid digestibility and true metabolizable energy corrected for nitrogen (TMEn). The essential amino acids, with the exception of methionine, were highly digestible with digestibility values of 80% to 90% (dry matter basis) for all selected pulse ingredients. BB grits had the lowest (P < 0.05) digestibility of arginine (86.5%) and histidine (80.6%) in contrast to GB (94.9% and 89.9%, respectively). The TMEn of GB was highest (P < 0.05) at 3.56 kcal/g compared with the other pulses. The DIAAS-like values for adult dogs were consistently the lowest for methionine for all pulses, making it the first-limiting amino acid in these ingredients. The DIAAS-like values for adult cats showed GL had lowest (P < 0.05) score in tryptophan compared with other pulses when using both AAFCO values and NRC recommended allowances as reference proteins. Methionine was the first-limiting amino acid for YP and tryptophan for GL. Based on macronutrient composition, protein quality, and amino acid digestibility, it can be concluded that pulse ingredients have the required nutritional characteristics to be viable protein sources in canine and feline foods. However, the use of complementary protein sources is recommended to counterbalance any potential limiting amino acids in pulse ingredients.

FLAXSEED CAKE IN PROTEIN FOOD ADDITIVES

2020 ◽  
Author(s):  
С.В. ЗВЕРЕВ ◽  
В.А. ЗУБЦОВ ◽  
В.Г. ЛОБАНОВ ◽  
Ю.Ф. РОСЛЯКОВ ◽  
Е.В. ГЛУХОВА
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Food Additives ◽  
Total Protein Content ◽  
Food Protein ◽  
Reference Protein ◽  
Amino Acid Score ◽  
Flax Seeds ◽  
Limiting Amino Acids

Исследована комплементарность белка льняного жмыха с белком ряда семян и зерна бобовых культур с целью создания пищевых добавок повышенной биологической ценности для использования в индивидуальном,функциональном, специализированном и спортивном питании. Произведен расчет аминокислотных скоров белка зерна продуктов растениеводства по отношению к эталонному белку ФАО/ВОЗ 2013 г. для человека (без учета усвояемости). По общему уровню белка и основным показателям льняной жмых существенно уступает только соевому жмыху и обрушенному люпину. Однако белок семян льна неполный. Разработан алгоритм оптимизации пятикомпонентных пищевых белковых систем по критерию максимума минимального аминокислотного скора лимитирующих аминокислот смеси. Программа расчета рационального состава белковых смесей выравнивает скоры лимитирующих аминокислот двух компонентов при ограничениях на содержание остальных трех компонентов. Определено, что идеальный белок (18–20%) получается при смешивании льняного жмыха с продуктами переработки зерна амаранта с долей льна (д. л.) 13–27%. Смеси льняного жмыха (ЛЖ) с нутом (Н) и люпином обрушенным (ЛО) с д. л. 37,0 и 6,5% соответственно имеют меньшее общее содержание белка, чем каждая компонента в отдельности, например, %: (ЛЖ + Н) 28, (ЛЖ + ЛО) 39, ЛЖ 31, ЛО 40. Однако смеси содержат большее количество эталонного белка: (ЛЖ + Н) 28%; (ЛЖ + ЛО) 37,6%. Для сравнения, содержание эталонного белка в продукте составляет, %: ЛЖ 23,3; ЛО 36,7; Н 25,0. Установлено, что высокое содержание белка в жмыхе, полученном из семян льна, позволяет создавать смеси с зернобобовыми продуктами, чей белок комплементарен льняному белку, иполучать пищевые системы с белком улучшенного аминокислотного профиля. The complementarity of flaxseed cake protein with the protein of a number of seeds and legume grains was studied in order to create food additives of increased biological value for use in individual, functional, specialized and sports nutrition. The calculation of the amino-acid score of the grain protein of some leguminous crops in relation to the FAO/WHO 2013 reference protein for humans (excluding digestibility) was made. In terms of the overall protein level and main indicators, flaxseed cake is significantly inferior only to soy cake and collapsed lupine. However, the protein of flax seeds is incomplete. An algorithm for optimizing five-component food protein systems based on the criterion of the maximum of the minimum amino-acid score of the limiting amino acids of the mixture is developed. The program for calculating the rational composition of protein mixtures equalizes the scores of limiting amino acids of two components with restrictions on the content of the other three components. It was determined that the ideal protein (18–20%) is obtained by mixing flaxseed cake with products of amaranth grain processing with a proportion of flax of 13–27%. Mixtures of flaxseed cake (FC) with chickpeas (Ch) and collapsed lupine (CL) with a proportion of flax of 37,0 and 6,5%, respectively, have a lower total protein content than each component separately, for example, %: (FC + Ch) 28, (FC + CL) 39, FC 31, CL 40. However, the mixtures contain more of the reference protein: (FC + Ch) 28%; (FC + CL) 37,6%. For comparison, the reference protein content in the product is, %: FC 23,3; CL 36,7; Ch 25,0. It was found that the high protein content in the cake obtained from flax seeds makes it possible to create mixtures with leguminous products whose protein is complementary to flaxseed protein, and to obtain food systems with protein of an improved amino acid profile.

scholarly journals Use of precision-fed cecectomized rooster assay and digestible indispensable amino acid scores to characterize plant- and yeast-concentrated proteins for inclusion in canine and feline diets1,2

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Lauren M Reilly ◽  
Patrick C von Schaumburg ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Pamela L Utterback ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Quality ◽  
Essential Amino Acids ◽  
Protein Sources ◽  
Protein Concentrates ◽  
Indispensable Amino Acid ◽  
Limiting Amino Acid ◽  
Adult Cats ◽  
Novel Protein

Abstract Increased consumer interest in high-quality and novel protein sources has driven the demand for the inclusion of protein-rich ingredients in companion animal diets. Novel protein concentrates, with protein contents of at least 50%, have been used to satisfy these consumer demands. However, minimal information is available regarding the macronutrient composition and protein quality of these ingredients that is needed for proper formulation of pet foods. Therefore, the objectives of this study were to determine the macronutrient and amino acid compositions, standardized amino acid digestibility according to the precision-fed rooster assay, and protein quality using digestible indispensable amino acid score (DIAAS like) of pea protein (PP), potato protein (POP), faba bean protein (FBP), soy protein concentrate (SPC), and dried yeast (DY). Precision-fed rooster assays were conducted using cecectomized roosters to calculate standardized amino acid digestibility and true metabolizable energy corrected for nitrogen (TMEn). For all five protein concentrates, all essential amino acids were highly digestible (88.0% to 96.3%, dry matter basis) with differences (P < 0.05) in only lysine, methionine, and tryptophan digestibilities. The TMEn values were highest for POP (4.22 kcal/g) and DY (3.61 kcal/g). The DIAAS-like values for adult dogs indicated that methionine was the first-limiting amino acid in all protein concentrates except POP, where the first-limiting amino acid was tryptophan. Using Association of American Feed Control Officials (AAFCO)-recommended values for adult cats, DIAAS-like values for methionine were lowest (P < 0.05) for FBP at 81.5%, with all other amino acids for all protein concentrates over 100%. The National Research Council (NRC)-recommended allowances for adult cats indicated that DIAAS-like methionine values for PP (92.7%) and FBP (73.8%) were significantly lower (P < 0.05) with these being the first-limiting amino acids, with the remaining amino acids above 100% for the other protein concentrates. The protein quality and high essential amino acid digestibility of these protein concentrates indicate that they would be viable protein sources in canine and feline diets. However, additional complementary protein sources should be included to meet the requirements of all essential amino acids.

scholarly journals Amino acid profile and scores of some selected traditional diets commonly consumed in northwest zone, Nigeria

2022 ◽  
Vol 6 (13) ◽  
pp. 22-28
Author(s):  
Maryam Abdulkadir Dangambo ◽  
Adamu Jibrin Alhassan ◽  
Atiku Muhammad Kano ◽  
Hafiz Abubakar ◽  
Zinat Suleiman Muhammad
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Quality ◽  
Normal Growth ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Groundnut Oil ◽  
Standard Amino Acid ◽  
Amino Acid Score ◽  
Traditional Diets

Background and aims: Amino acids composition of local and regional dietary proteins determines the protein quality. The study was aimed at evaluating amino acid profiles of some selected traditional diets commonly consumed in Jigawa (JG), Kano (KN) and Katsina (KT) States, Northwest Zone-Nigeria. Methods: The selected prepared diets (four per state) include; JG Tuwon masara using white maize served with Kuka soup (JG TMW SWKS), JG Tuwon masara using yellow maize served with Kuka soup (JG TMY SWKS), JG Danwake served with groundnut oil and pepper (JG Danwake SWGOP), JG Moimoi, KN Tuwon masara using white maize served with Kuka soup (KN TMW SWKS), KN Tuwon masara using yellow maize served with Kuka soup (KN TMY SWKS), KN rice and beans served with groundnut oil and pepper (KN Rice and Beans SWGOP), KN Danwake served with groundnut oil and pepper (KN Danwake SWGOP), KT Tuwon masara using white maize served with Kuka soup (KT TMW SWKS), KT Tuwon masara using yellow maize served with Kuka soup (KT TMY SWKS), KT Danwake served with groundnut oil and pepper (KT Danwake SWGOP) and KT Dambu. The preparations were dried and grounded into powdered form and analyzed using standard methods. Results: The amino acid profile of the diets consumed in the three states show higher content of total non-essential amino acids (NEAA) compared with essential amino acids (EAA). The amino acid score of the prepared diets though higher than the standard amino acid score by WHO/FAO/UNU (2007), may support the normal growth of all age groups except lysine. The limiting amino acid score was found to be either lysine or SAA (cysteine + methionine) in all the 3 states’ diets. Histidine was found to have the highest score in all the diets except in JG Moimoi and KN Danwake served with groundnut oil and pepper (SWGOP) respectively. The low levels of lysine and SAA in some of the diets may be due to low contents of these amino acids in the ingredients used. Conclusion: This study showed that, the amino acid score of the prepared diets though higher than the standard amino acid score established by WHO/FAO/UNU may support the normal growth of infants, children as well as adults except lysine. Keywords: traditional diets, amino acids, amino acid profile, amino acid score.

scholarly journals Are Dietary Amino Acids or Protein Quality Associated with Infant Length Gain from 6 to 12 Months in Rural Malawi? (P10-010-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Andrew Matchado ◽  
Kathryn Dewey ◽  
Christine Stewart ◽  
Per Ashorn ◽  
Ulla Ashorn ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Breast Milk ◽  
Protein Quality ◽  
Complementary Food ◽  
Complementary Foods ◽  
Acid Intake ◽  
Dietary Amino Acid ◽  
Amino Acid Intake ◽  
Length Gain

Abstract Objectives 1) to estimate the probability of inadequate amino acid intake among infants 9–10 months of age in rural Malawi 2) to evaluate whether dietary amino acid intake or protein quality are associated with length gain from 6 to 12 months of age Methods We assessed total amino acid intake from breast milk and complementary foods in 285 infants. Breast milk intake and complementary foods were estimated using dose-to-mother deuterium oxide dilution method and repeat 4-pass interactive 24-hour recall interviews, respectively. Amino acid composition values were taken from FAO human milk profile, Tanzania Food Composition table and International Minilist. Protein quality was estimated using Digestible Indispensable Amino Acid Score (DIAAS). Probability of intake below Estimated Average Requirement (EAR) for each amino acid was estimated using National Cancer Institute (NCI) method. We estimated protein quality of complementary food using median DIAAS. We assumed a DIAAS of ≥0.75 to represent a diet or food with good protein quality. Relationships between amino acid intake or protein quality with length gain were assessed using regression models. Length was measured at 6 and 12 months of age and length for age z-score (LAZ) velocity was calculated (ΔLAZ/months). Results The probability of inadequate amino acid intake from breast milk and complementary food that included a lipid-based nutrient supplement (LNS) was 3% for lysine, 0% for tryptophan, threonine, valine, histidine, isoleucine, leucine, sulfur containing amino acids (SAA), and aromatic amino acids (AAA). Without LNS, the probability was 7% for lysine and 0–2% for the other amino acids. The median (interquartile range) DIAAS for complementary food with and without LNS was 0.70 (0.28) and 0.64 (0.32), respectively. Dietary amino acid intake and protein quality were not significantly associated with length gain velocity from 6 to 12 months even after adjusting for confounding factors. Conclusions The prevalence of inadequate amino acid intake in 9–10 months old infants in rural Malawi is very low. However, in conditions of frequent clinical or sub-clinical infections this situation may be different. Linear growth at 6–12 months does not appear to be limited by dietary amino acid intake or protein quality in this setting. Funding Sources The Bill & Melinda Gates Foundation.

scholarly journals Rabbit Carcasses for Use in Feline Diets: Amino Acid Concentrations in Fresh and Frozen Carcasses With and Without Gastrointestinal Tracts

2021 ◽  
Vol 7 ◽  
Author(s):  
Tammy J. Owens ◽  
Andrea J. Fascetti ◽  
C. Christopher Calvert ◽  
Jennifer A. Larsen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Protein Body ◽  
National Research Council ◽  
Body Water ◽  
Taurine Supplementation ◽  
Frozen Ground ◽  
Taurine Deficiency ◽  
Amino Acid Concentrations

Whole-prey diets for exotic feline species are common, and this practice has also increased in popularity for domestic cats. However, prior analyses of prey indicate possible essential amino acid inadequacy, and dilated cardiomyopathy from taurine deficiency was reported in cats fed whole ground rabbit. Crude protein, body water, and amino acid concentrations were evaluated in fresh and frozen ground rabbits with (n=10) or without (n = 10) gastrointestinal tracts. Amino acids were greater in fresh samples without gastrointestinal tracts (p < 0.05) except taurine, glycine, and cysteine. When normalized for protein content, only glutamate, alanine, methionine, isoleucine, tyrosine, lysine, histidine, and arginine were greater in fresh rabbits without gastrointestinal tracts (g/16 g N basis; p < 0.05). Freezing at −18°C for 30 days had no effect on crude protein or body water content. After freezing, only methionine was lower and only proline was higher when gastrointestinal tracts were omitted (g/16 g N basis; p < 0.05). Regardless, all essential amino acids except taurine exceeded Association of American Feed Control Officials and National Research Council nutrient recommendations for all feline life stages. In contrast, there was minimal impact of treatment on taurine concentrations. However, although feline taurine requirements for prey and other raw or fresh food diets remain undefined, none of the rabbit samples met any recommendation for taurine concentrations for commercial canned or dry extruded diets, ranging from 20 to 90% of the minimum values. Taurine supplementation is recommended when feeding rabbit to cats. Determination of taurine requirements of cats fed whole-prey diets is warranted.

scholarly journals Apparent ileal digestibility of amino acids and metabolisable energy value in grains for broilers

2008 ◽  
Vol 14 (4) ◽  
pp. 325 ◽  
Author(s):  
S. PERTTILÄ ◽  
J. VALAJA ◽  
T. JALAVA
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Dry Matter ◽  
Nitrogen Excretion ◽  
Ileal Digestibility ◽  
Metabolisable Energy ◽  
Feed Protein ◽  
Digestible Amino Acids

Using ileal digestible amino acids in feed optimising will intensify feed protein utilizing and decrease nitrogen excretion to the environment. The study determined the apparent ileal digestibility (AID) coefficients of amino acids in barley, wheat, oats, triticale, maize, and dehulled oats in the diets of 180 Ross broiler chickens (aged 24–35 days). The birds were fed semi-purified diets that contained grain as the sole protein source and chromium-mordanted straw as an indigestible marker. The AID coefficients of the nutrients were assessed using the slaughter technique, and the apparent metabolisable energy (AME) was determined using total excreta collection. The ileal digestibility of the dry matter and organic matter were the highest in maize. The AME of maize was higher than that of other cereals. The ileal digestibility of crude protein was higher in wheat than that in barley, oats and dehulled oats. The AME of wheat was similar to that of barley and oats but lower than that of triticale and dehulled oats. The amino acid AID was highest in wheat (0.86) and triticale (0.85) and lowest in oats (0.79) and barley 0.77). The average amino acid AID was 0.81 in dehulled oats. The threonine AID was the same in all tested ingredients. The lysine, methionine, and cystine AID coefficients were 0.81, 0.79, and 0.71 respectively for barley; 0.86, 0.84, and 0.38 respectively for oats; 0.87, 0.86, and 0.53 respectively for dehulled oats; 0.84, 0.90, and 0.66 respectively for maize; 0.89, 0.88, and 0.77 respectively for triticale; and 0.87, 0.85, and 0.71 respectively for wheat. Results indicated that AME –values of domestic grains (barley, oats and wheat) are in the same level. Especially, low AME value of wheat needs further investigation.;

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