scholarly journals PSIII-10 Effect of WUR genotype on resilience to a polymicrobial natural disease challenge in pigs

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 165-165
Author(s):  
Ryan L Jeon ◽  
Austin M Putz ◽  
Michael Dyck ◽  
John C S Harding ◽  
Frederic Fortin ◽  
...  
Keyword(s):  
Mixed Model ◽  
Linear Mixed Model ◽  
Flow System ◽  
Average Daily Gain ◽  
Nucleotide Polymorphism ◽  
Swine Industry ◽  
Single Nucleotide ◽  
System A ◽  
Limited Effectiveness ◽  
Daily Gain

Abstract Porcine Reproductive and Respiratory Syndrome (PRRS) is caused by a virus that poses a threat to the global swine industry, costing the U.S. industry over 664 million dollars annually. Vaccination has limited effectiveness due to the virus’ antigenic and genetic shifts. Leveraging genetics to develop more resilient swine can, however, mitigate the effects of PRRS. Previous studies identified a Single Nucleotide Polymorphism (SNP) near the GBP5 gene (WUR) that was associated with resistance and resilience to PRRS, with the G allele being favorable over A. The objective of this study was to determine whether the WUR SNP is also associated with resilience to a polymicrobial natural disease challenge. Using a continuous flow system, a new batch of 60-75 naïve Yorkshire x Landrace nursery piglets was introduced every three weeks into a natural challenge facility that was initially seeded with multiple diseases, including PRRS. Traits recorded were growth rate, feed intake, backfat, loin depth, veterinary treatments, and mortality. Pigs were genotyped using a 600K SNP chip. Data from 2133 pigs were analyzed using a univariate linear mixed model that included, pen, litter, and animal genetics as random effects and WUR genotype as a fixed effect. Frequencies were 0.85, 0.14, and 0.01 for AA, AG, and GG. The G allele was favorable for most traits, with the contrast of AA vs AG significant for average daily gain in the nursery (0.339 vs 0.365 kg/d, p = 0.013) and number of treatments (2.48 vs 2.16 over 180 days, p = 0.072). Mortality rate was 26.0% for AA and 23.8% for AG (not significantly different). In conclusion, the G allele at the WUR SNP is potentially also associated with resilience to multi-factorial disease. Funded by Genome Canada, Genome Alberta, Genome Prairie, PigGen Canada, and USDA-NIFA.

Relationship among MS4A8 expression, its variants, and the immune response in a porcine model of Salmonella

2018 ◽  
Vol 98 (4) ◽  
pp. 778-786
Author(s):  
Tinghua Huang ◽  
Xiali Huang ◽  
Bomei Shi ◽  
Xiongyan Liang ◽  
Jingbo Luo ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Average Daily Gain ◽  
Carrier Status ◽  
Nucleotide Polymorphism ◽  
Swine Industry ◽  
Single Nucleotide ◽  
Fecal Shedding ◽  
Leukocyte Counts ◽  
Membrane Spanning ◽  
Daily Gain

Salmonella colonization often establishes carrier status in infected animals, which decreases their performance. Salmonella-carrying pigs shed large amounts of bacteria in their feces, and thus they have a negative economic impact on the swine industry. The MS4A8 gene (membrane-spanning 4-domains A8) was significantly activated, by up to 119-fold, in peripheral blood after Salmonella inoculation of pigs. The present study analyzed the correlation of peripheral blood expression level and a genetic variant of porcine MS4A8 with Salmonella-infection traits. The result indicated that MS4A8 expression levels correlated significantly with Salmonella shedding counts. Both the expression of MS4A8 and fecal shedding counts correlated with leukocytes, lymphocytes, monocytes, segmented neutrophils, and banded neutrophils. A novel single nucleotide polymorphism of porcine MS4A8 (nonsynonymous, Val > Ala) was associated with Salmonella shedding counts and average daily gain (ADG) of body weight. The TT genotype had higher fecal shedding counts, leukocyte counts, and lymphocyte counts than the TC and CC genotypes. The CC genotype had higher level of ADG than the TC and TT genotype (p < 0.05). Those results indicated that MS4A8 is intriguing and could be used as a prospective genetic marker for Salmonella susceptibility.

205 Dietary Strategies to Limit Average Daily Gain of Late Finishing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 64-64
Author(s):  
Devin Goehring ◽  
Katherine A McCormick ◽  
Julie Mahoney ◽  
Rodney Hinson
Keyword(s):  
Mixed Model ◽  
Linear Mixed Model ◽  
Block Design ◽  
Average Daily Gain ◽  
Finishing Pigs ◽  
Compensatory Gain ◽  
And Gender ◽  
Dietary Treatments ◽  
Dietary Strategies ◽  
Daily Gain

Abstract A total of 2,164 pigs [DNA610 x DNA241; initially 100.7 ± 2.14 kg body weight (BW)] were used in a 54.1 ± 3.85-day study to determine dietary strategies to limit average daily gain (ADG) in late finishing pigs raised in a commercial environment. Mixed-sex pens (20.0 ± 0.85 pigs/pen) balanced by initial BW and gender ratio were randomly assigned to 1 of 4 dietary treatments fed ad libitum with 27 replications each: (1) Nutritionally replete corn soybean-meal control (CTRL), (2) Treatment 1 with 21% reduced lysine and other amino acids (AAR), (3) Corn/vitamin/mineral diet (CVM), and (4) Treatment 3 fed for 21 days then switched to CTRL. Data were analyzed as a randomized complete block design using a linear mixed model. Relative to CTRL, feeding AAR or CVM for 21 days reduced ADG (P &lt; 0.001), average daily feed intake (ADFI; P = 0.005), and gain-to-feed (G:F, P &lt; 0.001). Over 55-days, AAR and CVM diets reduced (P &lt; 0.001) cumulative ADG, ADFI, G:F, and final BW compared to CTRL. Transitioning to CTRL diet from CVM diet after 21-days increased ADG (P &lt; 0.001) and improved G:F (P &lt; 0.001) from day 21 to marketing compared to 55-day CTRL, AAR, and CVM; nevertheless, the compensatory CTRL period was insufficient for the 21-day CVM fed pigs to fully compensate resulting in reduced cumulative BW, ADG (P &lt; 0.001) and poorer G:F (P &lt; 0.001) than the 55-day CTRL. All holding strategies decreased (P &lt; 0.001) carcass yield, hot carcass weight (HCW), and loin depth compared to CTRL. Utilizing a CVM diet for 21 or 55 days increased (P &lt; 0.001) backfat compared to CTRL or AAR. In summary, utilizing lysine deficient or corn/vitamin/mineral diets can limit ADG along with poorer G:F. Transitioning to a non-holding diet after feeding a corn/vitamin/mineral diet for 21-days resulted in compensatory gain, but reductions in growth performance and carcass traits remained evident.

scholarly journals Caveolin-1 rs4730751 single-nucleotide polymorphism may not influence kidney transplant allograft survival

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mehdi Maanaoui ◽  
Rémi Lenain ◽  
Aghilès Hamroun ◽  
Cynthia Van der Hauwaert ◽  
Benjamin Lopez ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Graft Survival ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Graft Loss ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Caveolin 1 ◽  
Significant Difference

Abstract Caveolin-1 is a protein (encoded by the CAV1 gene) supposedly harboring a protective effect against fibrosis. CAV1 rs4730751 single nucleotide polymorphism (SNP) AA genotype was initially associated with lower graft survival compared to non-AA. However, subsequent studies could not find the same effect. CAV1 rs4730751 SNP was investigated on 918 kidney donors. Multivariate Cox-model analyses were performed to evaluate risk factors for graft loss. Longitudinal changes on long-term estimated glomerular filtration rate (eGFRs) were evaluated with a linear mixed model. Histopathological findings from protocolled biopsies after 3 months post transplantation were also analyzed. Donor CAV1 rs4730751 genotyping proportions were 7.1% for AA, 41.6% for AC and 51.3% for CC. The AA genotype, compared to non-AA, was not associated with lower graft survival censored or not for death (multivariate analysis: HR = 1.23 [0.74–2.05] and HR = 1.27 [0.84–1.92]). Linear mixed model on long-term eGFRs revealed also no significant difference according to the genotype, yet we observed a trend. AA genotype was also not associated with a higher degree of fibrosis index on protocolled kidney biopsies at 3 months. To conclude, donor CAV1 rs4730751 SNP may impact on kidney transplantation outcomes, but this study could not confirm this hypothesis.

scholarly journals PSIII-5 Effects of ingredient composition of oral drenches in small piglets post-farrowing on average daily gain and pre-wean mortality

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 238-238
Author(s):  
Olivia Harrison ◽  
Samantha Tauer ◽  
Brent Frederick
Keyword(s):  
Birth Weight ◽  
Average Daily Gain ◽  
High Energy ◽  
Experimental Unit ◽  
Swine Industry ◽  
Bioactive Proteins ◽  
Randomized Design ◽  
Significant Difference ◽  
Key Factor ◽  
Daily Gain

Abstract Number of pigs born alive has been a key factor of the increasing efficiency of the U.S. swine industry. However, with increased pigs in the uterus, birth weight has been negatively impacted, with more small or at-risk pigs being born per litter. In order to overcome these changes, a study testing three commercial oral drenches against a control to determine which would increase average daily gain and decrease preweaning mortality. In a completely randomized design, 877 one-day-old suckling pigs from a high-health farm were selected for the experiment if they appeared to be in the bottom 20% of bodyweight compared to their contemporaries. Selected pigs were given one of four drenching treatments: 1) none (control), 2) bioactive proteins (BP), 3) high energy sugars (HES), and 4) immunoglobulins (IgY). Pigs were weighed on d 1 and d 19 of age (weaning), with mortality tracked during the suckling period. Data were analyzed using SAS v 9.4 (Cary, NC), with pig as the experimental unit and an accepted alpha of 0.05. Treatment had no detected effect on birth weight, weaning weight, ADG, or mortality (P = 0.79, 0.96, 0.86, 0.38 respectively). Likewise, statistical contrasts were used to determine there was no detected impact (P &gt; 0.10) of drench, regardless of type, compared to the control in any measured response criteria. Interesting, pigs drenched with BP or IgY had numerically lower preweaning mortality (11.2 and 11.5% respectively), than those administered the control or HES (15.4 and 15.2%, respectively). In conclusion, this experiment showed no significant difference in the performance between piglets given no product vs. those drenched with bioactive proteins, high energy sugars, or immunoglobulins. However, additional research is warranted with greater replication or disease stressors to better understand if oral drenches may improve preweaning performance or mortality in different situations.

Analysis of porcine OSBPL5 gene allelic expression in skeletal muscle and association of a single-nucleotide polymorphism in the coding region with production traits

2019 ◽  
Vol 99 (4) ◽  
pp. 914-920
Author(s):  
Meng Wang ◽  
Deli Wei ◽  
Guiling Cao ◽  
Guiyu Zhu ◽  
Yunliang Jiang
Keyword(s):  
Skeletal Muscle ◽  
Single Nucleotide Polymorphism ◽  
Growth Regulation ◽  
Average Daily Gain ◽  
Allelic Expression ◽  
Production Traits ◽  
Coding Region ◽  
Nucleotide Polymorphism ◽  
Specific Expression ◽  
Single Nucleotide

Genes that exhibit allelic expression imbalance and imprinted genes play important roles in the survival of the embryo and postnatal growth regulation. In this study, the porcine oxysterol-binding protein-related 5 (OSBPL5) gene was examined, and the 2140G>A mutation (rs318687202) was found in its coding region by a comparison of Laiwu and Landrace pigs. By allele-specific expression analysis based on a specific single-nucleotide polymorphism (SNP), the imprinting status of OSBPL5 gene in skeletal muscle from both neonate and adult pigs was determined. The results showed that the OSBPL5 was paternally imprinted in skeletal muscle from adults but biallelically expressed with predominantly maternal imprinting in neonates. The distribution of the 2140G>A SNP in four pig populations was analyzed, which showed that GG genotype was dominant in Duroc and Dapulian populations, whereas the AG genotype was dominant in Junmu-1 and Laiwu populations. Pigs with the GG genotype had significantly larger litters and greater cannon bone circumferences but a lower average daily gain than pigs with the AA genotype. In conclusion, we determined the difference in the allelic expression of OSBPL5 between adult and neonate pigs and identified an SNP in its coding region that is associated with production traits.

Effect of dietary magnesium supplementation in alleviating stress associated with road transportation in weaned lambs

2015 ◽  
Vol 55 (2) ◽  
pp. 219 ◽  
Author(s):  
J. L. Pye ◽  
R. E. Doyle ◽  
M. A. Friend ◽  
M. S. Bhanugopan
Keyword(s):  
Feed Intake ◽  
Stress Responses ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Serum Cortisol ◽  
Repeated Measure ◽  
Control Group ◽  
Road Transportation

Magnesium (Mg) supplementation has shown to modulate the stress responses to transport in other species. An experiment was designed to evaluate the effect of Mg for alleviating stress associated with road transportation in lambs. Two groups (n = 18 each) of 4-month old male crossbred lambs (24 ± 4.5 kg) were fed a basal diet comprising oaten hay, pellets and barley, that contained 0.17% Mg. One group was supplemented with magnesium oxide, such that dietary Mg was 0.41%. At the end of the 2-week supplementation period, the lambs were transported by road for 8 h. Blood samples were collected before supplementation, 1 h before transport, within 0.5 h post-transport, at 2 h post-transport and 5 days post-transport. Data were analysed using a repeated-measure analysis of variance and linear mixed model with treatment within time interaction. Supplementation with dietary Mg in the form of MgO increased serum Mg concentrations (1.16 mmol/L ± 0.012, compared with 1.09 mmol/L ± 0.013 in the control group; P < 0.05), but had no effect on alleviating stress during transportation. Transport resulted in a significant increase in serum cortisol, which returned to pre-transport levels 2 h after transport. The serum β-hydroxybutyrate was significantly higher 5 days after transport. The post-transport average feed intake and the average daily gain were significantly lower than pre-transport values, suggesting that the stress of transportation resulted in a decrease in feed intake post-transport, which could have implications on liveweight gain of animals after transportation.

Effect of feed particle size and dietary melatonin supplementation on gastric ulcers in swine

1999 ◽  
Vol 79 (2) ◽  
pp. 179-185 ◽  
Author(s):  
H. L. Ayles ◽  
R. M. Friendship ◽  
G. A. Bubenik ◽  
R. O. Ball
Keyword(s):  
Particle Size ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Endoscopic Examination ◽  
Growing Pigs ◽  
Feed Consumption ◽  
Gastric Ulcers ◽  
Swine Industry ◽  
Daily Gain ◽  
Growth Experiments

Gastric ulcers may be an underrecognized problem in the swine industry. An experiment was conducted to evaluate the effects of dietary melatonin supplementation and particle size on prevalence and severity of ulcers in growing pigs. Sixty-four Yorkshire barrows and gilts (29.5 ± 1.0 kg) received either a finely ground (763 ± 2.18 µm) or a coarsely ground (953 ± 2.29 µm) corn–wheat diet, either with or without added melatonin (5 mg kg−1). One half of the pigs underwent endoscopic examination to assess the severity of ulcers on days 4 and 25. Growth performance and feed consumption were monitored throughout the experiment. All pigs were slaughtered on day 28, and ulcers were assessed postmortem. Endoscopy at the beginning of the experiment found that 53% of the pigs already had some degree of ulceration. Pigs fed the fine diet had greater average daily gain and average daily feed intake than pigs fed the coarse diet (P < 0.05), but particle size did not significantly affect prevalence of stomach ulcers. Melatonin supplementation increased the apparent digestibility of crude protein (P < 0.04) and dry matter (P < 0.08) of the diets and decreased the prevalence of ulcers (P < 0.05) but did not significantly affect growth performance. Pigs that received melatonin had higher levels of melatonin (P < 0.01) in most gastrointestinal tissues, with highest concentrations found in the stomach (P < 0.001). Pigs that did not have ulcers had a higher concentration of melatonin in the plasma and stomach than pigs with ulcers (P < 0.05). Melatonin may be useful in decreasing ulcers. Initial ulcer condition may be a significant source of variation in growth experiments. Key words: Pigs, gastric ulcers, particle size, melatonin, gastrointestinal tract

scholarly journals PSIII-14 Performance differences between two groups of commercial pigs following experimental infection with porcine reproductive and respiratory syndrome virus (PRRS) virus 1-7-4

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 165-166
Author(s):  
Erin Little ◽  
Jenelle Dunkelberger ◽  
Dan Hanson ◽  
John Eggert ◽  
Michael Gonda ◽  
...  
Keyword(s):  
Feed Intake ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Average Daily Gain ◽  
Random Effect ◽  
Experimental Unit ◽  
Respiratory Syndrome Virus ◽  
Standard Index ◽  
Performance Differences ◽  
Significant Difference

Abstract Porcine reproductive and respiratory syndrome virus (PRRSv) is the costliest swine disease of North America. Vaccines and management alone have not been effective at controlling this disease and genetic selection may be a complimentary approach to controlling PRRSv-infection. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSv 1-7-4: 1) pigs sired by boars selected based on a standard index (STD), which emphasizes feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (EXP), with emphasis on feed intake, piglet vitality, and robustness. Pigs (n~730 per group) were housed in a commercial research wean-to-finish barn. Average daily gain (ADG) and average daily feed intake (ADFI) were recorded from birth-to-slaughter. At weaning, pigs were placed in pens by group and balanced by sex. To facilitate collection of feed intake data, the experimental unit was pen, with 27 pens representing each group. Four weeks post-wean, all pigs were experimentally infected with 2mL of 1-7-4 PRRSv at 3.5 logs of TCID50 per mL. Statistical analyses were performed using a linear mixed model with group (STD or EXP) as a fixed effect and block as a random effect. No significant difference was detected between groups for wean-to-finish or 0 to 42 days post-infection (dpi) ADG. The ADFI was 0.06 kg/day greater for the EXP group than the STD group from 0 to 42 dpi (P = 0.01). Feed conversion ratio (FCR) was 0.03 (P = 0.03) less for the EXP group from wean-to-finish and 0 to 42 dpi, respectively. Pigs sired by boars selected using the experimental index showed no significant difference in ADG, but had greater ADFI post-challenge, while pigs sired by boars selected based on the standard index had significantly better FC.

scholarly journals Adverse effects on growth performance and bone development in nursery pigs fed diets marginally deficient in phosphorus with increasing calcium to available phosphorus ratios

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Spenser L Becker ◽  
Stacie A Gould ◽  
Amy L Petry ◽  
Leah M Kellesvig ◽  
John F Patience
Keyword(s):  
Growth Performance ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Bone Development ◽  
Average Daily Gain ◽  
Experimental Period ◽  
Feed Ratio ◽  
Available Phosphorus ◽  
Nursery Pigs ◽  
The Impact

Abstract The objective of this experiment was to evaluate the growth performance and bone mineral content (BMC) of nursery pigs in response to increasing total calcium (Ca) to available phosphorus (aP) ratios in diets containing phytase (250 FTU/kg; Natuphos E, BASF, Florham Park, NJ). A total of 480 nursery pigs (body weight (BW) = 5.7 ± 0.6 kg) with 10 pigs per pen and 7 pens per treatment (6 pens fed 2.75:1 diet) were allotted to seven treatments consisting of increasing ratios of calcium to available phosphorus (Ca:aP): 1.25, 1.50, 1.75, 2.00, 2.25, 2.50, and 2.75. From day −7 to 0, pigs were fed a common diet. They were then fed the treatment diets during two experimental phases from day 1 to 14 and 15 to 28, respectively. Available P was formulated to 0.33% and 0.27% (approximately 90% of requirement) in dietary phases 1 and 2, respectively. BW, average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F) were determined. BMC of the femur was measured on day 28 on one pig per pen using dual x-ray absorptiometry. Data were analyzed as a linear mixed model using PROC MIXED (SAS, 9.3). Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing the Ca:aP. Over the 28-d experimental period, increasing Ca:aP resulted in a linear decrease in ADG (353, 338, 328, 304, 317, 291, and 280 g/d; P &lt; 0.01), ADFI (539, 528, 528, 500, 533, 512, and 489 g/d; P &lt; 0.05), and G:F (0.68, 0.66, 0.64, 0.62, 0.61, 0.59, and 0.58; P &lt; 0.01). Increasing Ca:aP also resulted in decreased BW on days 14 and 28 (P &lt; 0.01). The BMC of the femur decreased with increasing Ca:aP (6.2, 6.3, 5.7, 5.9, 5.5, 5.6, and 5.3 g; P &lt; 0.05). Regression analysis explained the impact of Ca:aP as follows on ADG (ADG [g/d] = 339 − 36x; r2 = 0.81), G:F (G:F = 0.61 – 0.03x; r2 = 0.72), and BMC (BMC [g] = 6.4 – 0.27x; r2 = 0.43), where x is the Ca:aP. In conclusion, all outcomes indicated that any level of calcium above the minimum used in this experiment impaired growth performance and skeletal development. Further research using even lower levels of dietary Ca is warranted.

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