scholarly journals PSVII-16 Dietary creatine and sulfur amino acids for finishing gilts

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 362-362
Author(s):  
Natália Y Sitanaka ◽  
Lucas Antonio C Esteves ◽  
Alice Murakami ◽  
Eliane Gasparino ◽  
Angélica Khatlab ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasma Concentrations ◽  
Blood Parameters ◽  
Plasma Creatinine ◽  
Sulfur Amino Acids ◽  
Creatinine Concentration ◽  
Creatine Transporter ◽  
Longissimus Dorsi Muscle ◽  
Plasma Creatinine Concentration

Abstract The objective of this study was to evaluate the effects of dietary creatine and SID methionine+cysteine (Met+Cys) levels on the performance, blood parameters and gene expression of the creatine transporter (SLC6A8) in finishing gilts. Forty gilts, averaging 75.26 ± 0.87 kg of initial weight, were distributed in a randomized blocks design in a 2x2 factorial scheme, consisting of two creatine monohydrate (CMH) supplementation levels (0.00 and 0.10%) and two levels of SID Met+Cys (0.40 and 0.44%, considering 0.44% as the requirement), with 10 replicates. DL methionine was used to ensure the dietary SID Met+Cys levels. Upon reaching a mean weight of 100± 5.85, blood was collected for the determination of urea, creatinine, lactate, glucose and homocysteine plasma concentrations. Afterwards, the gilts were slaughtered for the collection of Longissimus dorsi muscle samples, for further determination of the gene expression of the creatine transporter (SLC6A8). No interactions (P > 0.05) we observed between the CMH and SID Met+Cys on the performance and gene expression of the SLC6A8 transporter, and also were not affected (P > 0.05) by the dietary levels of CMH or SID Met+Cys, individually. However, there was an interaction (P = 0.03) between SID Met+Cys and CMH levels on the plasma creatinine concentration, showing a lower (P = 0.018) concentration (6.40 mg/dL) supplementing 0.10% CMH than not supplementing (8.96 mg/dL), only at 0.44% of SID Met+Cys. There were no interactions (P > 0.05) between SID Met+Cys and CMH on the other blood parameters, and also no individual effects were observed for the studied factors. It is concluded that supplementing 0.10% CMH reduced plasma creatinine concentration only at conventionally dietary SID Met+Cys level (0.44%), not affecting other blood parameters, growth performance and the gene expression of the creatine transporter SLC6A8 of finishing gilts.

Nitromethane interferes in assay of creatinine by the Jaffé reaction.

1989 ◽  
Vol 35 (8) ◽  
pp. 1772-1774 ◽  
Author(s):  
E A De Leacy ◽  
N N Brown ◽  
A E Clague
Keyword(s):  
Mixture Model ◽  
Model System ◽  
Plasma Creatinine ◽  
Enzymatic Method ◽  
Main Interest ◽  
Creatinine Concentration ◽  
Spectral Changes ◽  
Plasma Creatinine Concentration

Abstract A patient who ingested a methanol/nitromethane mixture (model airplane fuel) showed an apparent plasma creatinine concentration of 8.0 mmol/L by the Jaffé reaction when the actual creatinine concentration, as measured by a specific enzymatic method, was 0.09 mmol/L. This effect was due to nitromethane in the plasma. Interference by nitromethane with the determination of creatinine by reaction with alkaline picrate (the Jaffé reaction) has not previously been reported. When nitromethane was added to plasma, the apparent creatinine measured was linearly related to the amount of nitromethane added. Comparison of spectral changes occurring during creatinine/picrate and nitromethane/picrate reactions show substantial similarity, suggesting a similar structure for the products. Although the findings are of interest for both their toxicological and analytical implications, the main interest lies with their suggestion of a model system for future investigation of the Jaffé reaction.

Regulation of Plasma Creatinine Concentration by Use of a Servo Control System

1968 ◽  
Vol 14 (10) ◽  
pp. 944-959 ◽  
Author(s):  
Rodney B Harvey ◽  
James B Bassingthwaighte ◽  
Richard L Heppner
Keyword(s):  
Infusion Pump ◽  
The Body ◽  
Servo Control ◽  
Plasma Creatinine ◽  
Creatinine Concentration ◽  
Servo Control System ◽  
Plasma Creatinine Concentration ◽  
The Difference ◽  
Continuous Determination

Abstract A method for controlling the plasma creatinine concentration is described. This method uses continuous determination of the plasma creatinine concentration and a servomechanism which drives an infusion pump at a rate proportional to the difference between the actual and desired creatinine concentrations. The rate and volume of creatinine infusion necessary to maintain the desired plasma concentration provide information related to kidney function and the accumulation of creatinine in the body. Experiments using this technic and the method of data analysis are described. It has been shown that under certain conditions this technic may be used to estimate the renal clearance of creatinine and the size and nature of the space into which creatinine is distributed in the dog.

Production of Methylguanidine in Dogs with Acute and Chronic Renal Failure

1989 ◽  
Vol 77 (6) ◽  
pp. 637-641 ◽  
Author(s):  
David P. Brooks ◽  
Gerald R. Rhodes ◽  
Paul Woodward ◽  
Venkata K. Boppana ◽  
Francine M. Mallon ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Renal Insufficiency ◽  
Solid Phase ◽  
Plasma Concentrations ◽  
Tubular Secretion ◽  
Plasma Creatinine ◽  
Creatinine Concentration ◽  
Plasma Creatinine Concentration ◽  
Renal Tubular

1. Methylguanidine is a suspected uraemic toxin that accumulates in renal failure 2. We measured methylguanidine in the plasma of dogs with acute ischaemic-induced renal failure and in the plasma and urine of dogs with spontaneous chronic renal insufficiency, using a highly sensitive method involving solid-phase extraction followed by h.p.l.c. with post-column fluorescence detection 3. Constriction of the remaining renal artery of four uninephrectomized dogs for 90 min resulted in a significant (P < 0.01) increase in plasma creatinine concentration after 24 h (from 113 ± 3 to 303 ± 50 μmol/l; mean ± sem). Over the next 14 days, plasma creatinine fell towards baseline concentrations. Plasma methylguanidine also increased significantly (P < 0.05) 24 h after renal occlusion (from 0.16 ± 0.04 to 0.86 ± 0.32 μmol/l) and showed a similar pattern to the plasma creatinine concentration 4. In a further four dogs, administration of mannitol (2 g/kg) at the time of reperfusion significantly attenuated these responses 5. Dogs with chronic renal failure demonstrated increased plasma concentrations and urinary excretion of methylguanidine, and the levels appeared to be related to the severity of renal insufficiency. Thus, the dogs with the highest plasma creatinine concentrations and lowest creatinine clearances had the highest plasma methylguanidine concentrations. The clearance of methylguanidine exceeded that of creatinine, indicating that the toxin undergoes renal tubular secretion.

scholarly journals Plasma creatinine medians from patients partitioned by gender and age used as a tool for assessment of analytical stability at different concentrations

2019 ◽  
Vol 58 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Steen Ingemann Hansen ◽  
Per Hyltoft Petersen ◽  
Flemming Lund ◽  
Callum G. Fraser
Keyword(s):  
Systematic Errors ◽  
Reference Intervals ◽  
Plasma Creatinine ◽  
Creatinine Concentration ◽  
Medical Laboratories ◽  
Analytical Stability ◽  
Plasma Creatinine Concentration ◽  
Gender And Age ◽  
Performance Specifications

Abstract Background Monthly medians of patient results are useful in assessment of analytical quality in medical laboratories. Separate medians by gender makes it possible to generate two independent estimates of contemporaneous errors. However, for plasma creatinine, reference intervals (RIs) are different by gender and also higher over 70 years of age. Methods Daily, weekly and monthly patient medians were calculated from the raw data of plasma creatinine concentrations for males between 18 and 70 years, males >70 years, females between 18 and 70 years and females >70 years. Results The medians of the four groups were all closely associated, with similar patterns. The mean of percentage bias from each group defined the best estimate of bias. The maximum half-range (%) of the bias evaluations provided an estimate of the uncertainty comparable to the analytical performance specifications: thus, bias estimates could be classified as optimum, desirable or minimum quality. Conclusions Medians by gender and age are useful in assessment of analytical stability for plasma creatinine concentration ranging from 60 to 90 μmol/L. The daily medians are valuable in rapid detection of large systematic errors, the weekly medians in detecting minor systematic errors and monthly medians in assessment of long-term analytical stability.

Changes in Plasma Creatinine Concentration after Cardiac Anesthesia with Isoflurane, Propofol, or Sevoflurane

2001 ◽  
Vol 95 (4) ◽  
pp. 842-848 ◽  
Author(s):  
David A. Story ◽  
Stephanie Poustie ◽  
Guoming Liu ◽  
P. Larry McNicol
Keyword(s):  
Coronary Artery ◽  
Renal Impairment ◽  
Plasma Creatinine ◽  
Coronary Artery Surgery ◽  
Primary Analysis ◽  
Creatinine Concentration ◽  
Sevoflurane Group ◽  
Isoflurane Group ◽  
Plasma Creatinine Concentration ◽  
Median Maximum

Background Renal impairment often follows cardiac surgery. The authors investigated whether sevoflurane produces greater increases in plasma creatinine concentration than isoflurane or propofol after elective coronary artery surgery. Methods As part of maintenance anesthesia, including during cardiopulmonary bypass, patients were randomly allocated to receive one of three agents: isoflurane (n = 118), sevoflurane (n = 118), or propofol (n = 118). Fresh gas flows were 3 l/min. The preoperative plasma creatinine concentration was subtracted from the highest creatinine concentration in the first 3 postoperative days. A median maximum increase greater than 44 microM (0.5 mg/dl) was regarded as clinically important. Data were analyzed on an intention-to-treat basis. Subgroup analyses were performed on per-protocol patients and those with preoperative renal impairment (creatinine concentration &gt; 130 microM [1.47 mg/dl] or urea &gt; 7.7 mM [blood urea nitrogen, 21.6 mg/dl]). Results The differences between the groups were small, clinically unimportant, and not statistically significant for the primary analysis and subgroups. The proportions of patients with creatinine increases greater than 44 microM were 15% in the isoflurane group, 17% in the sevoflurane group, and 11% in the propofol group (P = 0.45). The median increases were 8 microM in the isoflurane group, 4 microM in the sevoflurane group, and 6 microM in the propofol group. The differences between the three median maximum increases were 1-4 microM (P &gt; 0.45). In the subgroup with preoperative renal impairment, the median increases were 10 microM in the isoflurane group, 15 microM in the sevoflurane group, and 5 microM in the propofol group (P = 0.72). Conclusions Sevoflurane did not produce greater increases in creatinine than isoflurane or propofol after elective coronary artery surgery.

Effect of water restriction and environmental temperatures on metabolic rate and physiological parameters in sheep

2000 ◽  
Vol 80 (1) ◽  
pp. 97-104 ◽  
Author(s):  
B. T. Li ◽  
R. J. Christopherson ◽  
S. J. Cosgrove
Keyword(s):  
Metabolic Rate ◽  
Heat Production ◽  
Plasma Osmolality ◽  
Blood Parameters ◽  
Water Restriction ◽  
Creatinine Concentration ◽  
Warm Environment ◽  
Plasma Creatinine Concentration ◽  
Hb Concentration ◽  
Environmental Temperatures

The hypothesis that water restriction reduces metabolic rate and contributes to energy conservation of sheep, and induces changes in blood parameters was tested. Four of eight adult sheep were housed in either a warm (24.8 ± 1.5 °C) or cold (0.4 ± 1.2 °C) environment and fed a diet of alfalfa pellets at 1.2 × maintenance. Each sheep was fasted with or without water according to a crossover design. Average heat production (HP) and rectal temperature (Tr) were higher (P < 0.05) in the cold than in the warm. Fasting decreased HP and Tr (P < 0.05). Water restriction had no additional effect on HP and Tr. Fasting and fasting plus water restriction influenced plasma osmolality and creatinine concentration. Plasma creatinine concentration was lower (P < 0.01) and haemoglobin (Hb) concentration higher in the cold than in the warm environment. Hb concentration was increased with water restriction (P < 0.01) in the warm environment. Plasma cortisol concentration was altered by fasting. Packed cell volume (PCV) in blood, plasma volume and plasma aldosterone were not affected by treatments. The results suggest that water restriction, per se, for 3 d does not suppress metabolic rate in sheep below that resulting from fasting alone. Key words: Heat production, sheep, temperature, water restriction

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