Effect of 3-mercaptopyruvate Sulfurtransferase Deficiency on the Development of Multiorgan Failure, Inflammation, and Wound Healing in Mice Subjected to Burn Injury

Abstract Objectives Studies suggest that a restrictive transfusion strategy is safe in burns, yet the efficacy of a restrictive transfusion policy in massive burn injury is uncertain. Our objective: compare outcomes between massive burn (≥60% total body surface area (TBSA) burn) and major (20–59% TBSA) burn using a restrictive or a liberal blood transfusion strategy. Methods Patients with burns ≥20% were block randomized by age and TBSA to a restrictive (transfuse hemoglobin <7 g/dL) or liberal (transfuse hemoglobin <10 g/dL) strategy throughout hospitalization. Data collected included demographics, infections, transfusions, and outcomes. Results Three hundred and forty-five patients received 7,054 units blood, 2,886 in massive and 4,168 in restrictive. Patients were similar in age, TBSA, and inhalation injury. The restrictive group received less blood (45.57 ± 47.63 vs. 77.16 ± 55.0, p < 0.03 massive; 11.0 ± 16.70 vs. 16.78 ± 17.39, p < 0.001) major). In massive burn, the restrictive group had fewer ventilator days (p < 0.05). Median ICU days and LOS were lower in the restrictive group; wound healing, mortality, and infection did not differ. No significant outcome differences occurred in the major (20–59%) group (p > 0.05). Conclusions: A restrictive transfusion strategy may be beneficial in massive burns in reducing ventilator days, ICU days and blood utilization, but does not decrease infection, mortality, hospital LOS or wound healing.

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Wound healing after cultured epithelial autografting in patients with massive burn injury: A cohort study

Journal of Plastic Reconstructive & Aesthetic Surgery ◽

10.1016/j.bjps.2018.11.003 ◽

2019 ◽

Vol 72 (3) ◽

pp. 427-437 ◽

Cited By ~ 5

Author(s):

Cheng Hean Lo ◽

Shiva Akbarzadeh ◽

Catriona McLean ◽

Andrew Ives ◽

Eldho Paul ◽

...

Keyword(s):

Wound Healing ◽

Cohort Study ◽

Burn Injury

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The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Polymers ◽

10.3390/polym13183116 ◽

2021 ◽

Vol 13 (18) ◽

pp. 3116

Author(s):

Thien Do ◽

Tien Nguyen ◽

Minh Ho ◽

Nghi Nguyen ◽

Thai Do ◽

...

Keyword(s):

Wound Healing ◽

Burn Injury ◽

Healing Process ◽

Bactericidal Effect ◽

Wound Healing Process ◽

Burn Wounds ◽

Partial Thickness Burn ◽

Tissue Damages

(1) Background: Wounds with damages to the subcutaneous are difficult to regenerate because of the tissue damages and complications such as bacterial infection. (2) Methods: In this study, we created burn wounds on pigs and investigated the efficacy of three biomaterials: polycaprolactone-gelatin-silver membrane (PCLGelAg) and two commercial burn dressings, Aquacel® Ag and UrgoTulTM silver sulfadiazine. In vitro long-term antibacterial property and in vivo wound healing performance were investigated. Agar diffusion assays were employed to evaluate bacterial inhibition at different time intervals. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill assays were used to compare antibacterial strength among samples. Second-degree burn wounds in the pig model were designed to evaluate the efficiency of all dressings in supporting the wound healing process. (3) Results: The results showed that PCLGelAg membrane was the most effective in killing both Gram-positive and Gram-negative bacteria bacteria with the lowest MBC value. All three dressings (PCLGelAg, Aquacel, and UrgoTul) exhibited bactericidal effect during the first 24 h, supported wound healing as well as prevented infection and inflammation. (4) Conclusions: The results suggest that the PCLGelAg membrane is a practical solution for the treatment of severe burn injury and other infection-related skin complications.

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The association between vitamin D levels and burn factors in different burn types

Burns & Trauma ◽

10.1093/burnst/tkaa018 ◽

2020 ◽

Vol 8 ◽

Author(s):

Yoon Soo Cho ◽

Cheong Hoon Seo ◽

So Young Joo ◽

Suk Hoon Ohn

Keyword(s):

Vitamin D ◽

Wound Healing ◽

Vitamin D Deficiency ◽

Burn Injury ◽

Linear Regression Analysis ◽

Burn Patients ◽

Rehabilitation Therapy ◽

Vitamin D Levels ◽

Flame Burn ◽

Intensive Rehabilitation

Abstract Background Patients with burns present with different clinical features depending on the types of burn injury and burn patients with lower levels of vitamin D have worse prognoses and more complications. The study aims to investigate the association between vitamin D levels and burn factors according to each burn type in relation to early intensive rehabilitation therapy initiated for inpatients with burns. Methods In this retrospective study, we enrolled 757 of 1716 inpatients who underwent rehabilitative therapy between May 2013 and April 2017. Burn types were divided into flame burn, electrical burn and other burns, including scalding, contact and chemical burns. Age, burned body surface area (BSA), wound healing time (WHT), length of hospital stay (LOS) and body mass index were analysed between vitamin D deficient and non-deficient patient groups using Student’s t-tests, or Mann-Whitney U test and among three burn types using one-way analysis of variance (ANOVA) or Kruskal-Wallis one-way ANOVA. The relationship between vitamin D levels and burn factors was evaluated using Pearson's or Spearman's correlation coefficient tests, and multiple linear regression analysis in different burn groups. Results In total, 88.9% patients were vitamin D deficient, and these patients had a larger burned BSA (p = 0.015) and longer WHT and LOS (all p < 0.001) than non-deficient patients. Burned BSA, WHT and vitamin D levels showed significant differences in their mean values according to three burn types (all p < 0.001). WHT was a communal factor significantly associated with vitamin D levels in all three burn types (p < 0.05). The WHT cut-off points to predict vitamin D deficiency were 55 days for flame burn (p < 0.001) and 62.5 days for electrical burn (p = 0.001). Conclusions WHT across all three burn types was a common factor associated with vitamin D levels for inpatients with burns who had undergone rehabilitative therapy. Electrical burn patients with vitamin D deficiency, even those with a low burned BSA percentage, showed prolonged wound healing over a two-month post-burn period. Independent of burned BSA, nutritional intervention concerning vitamin D in relation to burn wound healing should be considered to guide early initiation of intensive rehabilitation therapy.

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Effect of small molecular weight soybean protein-derived peptide supplementation on attenuating burn injury-induced inflammation and accelerating wound healing in a rat model

RSC Advances ◽

10.1039/c8ra09036j ◽

2019 ◽

Vol 9 (3) ◽

pp. 1247-1259 ◽

Cited By ~ 6

Author(s):

Fen Zhao ◽

Wei Liu ◽

Yonghui Yu ◽

Xinqi Liu ◽

Huinan Yin ◽

...

Keyword(s):

Molecular Weight ◽

Wound Healing ◽

Rat Model ◽

Burn Injury ◽

Soybean Protein ◽

Burn Injuries ◽

Small Molecular Weight

The populations most afflicted by burn injuries have limited abilities to support the significant specialized requirements and costs for acute and long-term burn injury care.

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Topical Wound Healing Activity of Myricetin Isolated from Tecomaria capensis v. aurea

Molecules ◽

10.3390/molecules25214870 ◽

2020 ◽

Vol 25 (21) ◽

pp. 4870

Author(s):

Abdelsamed I. Elshamy ◽

Naglaa M. Ammar ◽

Heba A. Hassan ◽

Walaa A. El-Kashak ◽

Salim S. Al-Rejaie ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Cytokines ◽

Group Treatment ◽

Wound Closure ◽

Burn Injury ◽

Control Group ◽

Albino Rats ◽

Large Area ◽

Blood Capillaries ◽

Tnf Α

Wounds and burn injury are major causes of death and disability worldwide. Myricetin is a common bioactive flavonoid isolated naturally from the plant kingdom. Herein, a topical application of naturally isolated myricetin from the shoots of Tecomaria capensis v. aurea on excisional wound healing that was performed in albino rats. The wounded rats were treated every day with 10 and 20% myricetin for 14 days. During the experiment, the wound closure percentage was estimated at days 0, 7, and 14. Effects of myricetin on the inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and cluster of differentiation 68 (CD68) in the serum were evaluated using immunosorbent assay kits. The percentage of wound closure and contraction was delayed in wounded rats (67.35%) and was remarkably increased after treatment of wounded rats with myricetin; the treatment with 20% myricetin was the most potent (98.76%). Histological findings exhibited that 10% myricetin caused the formation of a large area of scarring at the wound enclosure and stratified squamous epithelium without the formation of papillae as in the control group. Treatment with 20% myricetin exhibited less area of scarring at the wound enclosure as well as re-epithelialization with a high density of fibroblasts and blood capillaries in the wound. Level elevations of serum pro-inflammatory cytokines, IL-1β, and TNF-α and macrophage CD68 were decreased in wounded rats treated with myricetin. Thus, it can be suggested that the enhancements in inflammatory cytokines as well as systemic reorganization after myricetin treatment may be recommended to play a crucial part in the promotion of wound healing. The findings suggest that treatment with a higher dose of myricetin was better in improving wound curing in rats. It could serve as a potent anti-inflammatory agent and can be used as an adjunctive or alternative agent in the future.

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Ghrelin accelerates wound healing through GHS-R1a-mediated MAPK-NF-κB/GR signaling pathways in combined radiation and burn injury in rats

Scientific Reports ◽

10.1038/srep27499 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 16

Author(s):

Cong Liu ◽

Jiawei Huang ◽

Hong Li ◽

Zhangyou Yang ◽

Yiping Zeng ◽

...

Keyword(s):

Wound Healing ◽

Signaling Pathways ◽

Burn Injury

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Deep dermal burn injury results in scarless wound healing in the ovine fetus

Wound Repair and Regeneration ◽

10.1111/j.1067-1927.2005.130210.x ◽

2005 ◽

Vol 13 (2) ◽

pp. 189-197 ◽

Cited By ~ 20

Author(s):

John F. Fraser ◽

Leila Cuttle ◽

Margit Kempf ◽

Gael E. Phillips ◽

Peter K. O'Rourke ◽

...

Keyword(s):

Wound Healing ◽

Burn Injury ◽

Ovine Fetus

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The effect of dietary intake of antioxidant micronutrients on burn wound healing: a study in a tertiary health institution in a developing country

Burns & Trauma ◽

10.1186/s41038-015-0012-x ◽

2015 ◽

Vol 3 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Mary Adjepong ◽

Pius Agbenorku ◽

Patricia Brown ◽

Ibok Oduro

Keyword(s):

Wound Healing ◽

Vitamin C ◽

Dietary Intake ◽

Burn Injury ◽

Dietary Assessment ◽

Cross Sectional Study ◽

Burn Patients ◽

Burn Wound ◽

Cross Sectional ◽

Nutrient Analysis

Abstract Background Burn injury results in emotional stress affecting dietary intake and antioxidant micronutrient intake, which is known to have effects on recovery outcomes. The study aimed to assess dietary intake of antioxidant micronutrients and recovery outcomes of burn patients. Methods Questionnaires were administered to 40 burn patients at Komfo Anokye Teaching Hospital (Ghana) from January 1, 2014 to May 30, 2014. The data taken include anthropometric measurements and dietary assessment. Their nutrient intakes were assessed with the Nutrient Analysis Template. The average intakes were compared to the recommended daily allowance. Assessment of recovery was based on records of wound healing assessments and infection rates from the health practitioners. Results A cross-sectional study of 40 patients revealed an average total burn surface area (TBSA) of 31.4 %, where 70.0, 35.0, 75.0, 52.5, 12.5 and 32.5 % patients were deficient in vitamins A, C and E, zinc, copper and selenium, respectively and adequate amounts of vitamin C intake were related with significantly better wound healing progress. Positive wound healing outcomes were observed for patients with adequate vitamins A and E and zinc intake. Less infection presented among patients with adequate amount of vitamins A and C and zinc, but this was not observed for patients with adequate vitamin E, copper and selenium. Conclusions Most burn patients did not meet their dietary requirements for antioxidant micronutrients and this was due to meals not tailored to suit individual requirements. Adequacy of the antioxidants especially vitamin C resulted in positive wound healing outcomes. Hence, there is need for planned well-balanced meals of high vitamin C.

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