scholarly journals Phylogenetic Network of Mitochondrial COI Gene Sequences Distinguishes 10 Taxa Within the Neotropical Albitarsis Group (Diptera: Culicidae), Confirming the Separate Species Status of Anopheles albitarsis H (Diptera: Culicidae) and Revealing a Novel Lineage, Anopheles albitarsis J

2020 ◽  
Author(s):  
Maysa T Motoki ◽  
Yvonne-Marie Linton ◽  
Jan E Conn ◽  
Fredy Ruiz-Lopez ◽  
Richard C Wilkerson
Keyword(s):  
Isolation By Distance ◽  
Species Recognition ◽  
Divergence Time ◽  
Phylogenetic Network ◽  
Coi Gene ◽  
Mitochondrial Lineage ◽  
Separate Species ◽  
Mitochondrial Coi ◽  
Two Samples ◽  
Statistical Parsimony

Abstract The Neotropical Albitarsis Group is a complex assemblage of essentially isomorphic species which currently comprises eight recognized species—five formally described (Anopheles albitarsis Lynch-Arribalzaga, An. deaneorum Rosa-Freitas, An. janconnae Wilkerson and Sallum, An. marajoara Galvao and Damasceno, An. oryzalimnetes Wilkerson and Motoki) and three molecularly assigned (An. albitarsis F, G & I)—and one mitochondrial lineage (An. albitarsis H). To further explore species recognition within this important group, 658 base pairs of the mitochondrial DNA cytochrome oxidase subunit I (COI) were analyzed from 988 specimens from South America. We conducted statistical parsimony network analysis, generated estimates of haplotype, nucleotide, genetic differentiation, divergence time, and tested the effect of isolation by distance (IBD). Ten clusters were identified, which confirmed the validity of the eight previously determined species, and confirmed the specific status of the previous mitochondrial lineage An. albitarsis H. High levels of diversity were highlighted in two samples from Pará (= An. albitarsis J), which needs further exploration through additional sampling, but which may indicate another cryptic species. The highest intra-specific nucleotide diversity was observed in An. deaneorum, and the lowest in An. marajoara. Significant correlation between genetic and geographical distance was observed only in An. oryzalimnetes and An. albitarsis F. Divergence time within the Albitarsis Group was estimated at 0.58–2.25 Mya, during the Pleistocene. The COI barcode region was considered an effective marker for species recognition within the Albitarsis Group and a network approach was an analytical method to discriminate among species of this group.

scholarly journals Genetic analysis reveals Finnish Formica fennica populations do not form a separate genetic entity from F. exsecta

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6013 ◽  
Author(s):  
Sanja Maria Hakala ◽  
Perttu Seppä ◽  
Maria Heikkilä ◽  
Pekka Punttila ◽  
Jouni Sorvari ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Morphological Characters ◽  
Coi Gene ◽  
Species Status ◽  
Separate Species ◽  
Mitochondrial Coi ◽  
Recent Addition ◽  
Morphological Differences ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers

Coptoformica Müller, 1923 is a subgenus of Formica Linnaeus, 1758 that consists of c. a dozen species of ants that typically inhabit open grassy habitats and build small nest mounds. The most recent addition to the group is Formica fennica Seifert, 2000. The description was based on morphological characters, but the species status has not been confirmed by molecular methods. In this study, we use thirteen DNA microsatellite markers and a partial mitochondrial COI gene sequence to assess the species status of F. fennica, by comparing the genetic variation among samples identified as F. fennica and six other boreal Formica (Coptoformica) species. Most of the species studied form separate, discontinuous clusters in phylogenetic and spatial analyses with only little intraspecific genetic variation. However, both nuclear and mitochondrial markers fail to separate the species pair F. exsecta Nylander, 1846 and F. fennica despite established morphological differences. The genetic variation within the F. exsecta/fennica group is extensive, but reflects spatial rather than morphological differences. Finnish F. fennica populations studied so far should not be considered a separate species, but merely a morph of F. exsecta.

Resident and anadromous forms of arctic charr (Salvelinus alpinus) from North-East Europe: an example of high ecological variability without speciation

2019 ◽  
Vol 485 (2) ◽  
pp. 242-246
Author(s):  
A. A. Makhrov ◽  
I. N. Bolotov ◽  
V. M. Spitsyn ◽  
M. Yu. Gofarov ◽  
V. S. Artamonova
Keyword(s):  
Salvelinus Alpinus ◽  
Arctic Charr ◽  
Arctic Char ◽  
The Arctic ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Ecological Variability ◽  
North East ◽  
The North ◽  
Two Samples

Samples from 11 populations of the Arctic char of the North-European part of Russia belonging to the anadromous and resident forms and two samples from Lake Sobach'e (Taimyr) were studied. The nucleotide sequence of the mitochondrial COI gene was determined in 60 individuals. In the majority of populations, the same COI haplotype was found. In some populations of the resident chars, haplotypes differing from the widespread haplotype in a single nucleotide substitution were found. The obtained genetic data give no reason to distinguish the resident form of the Arctic char from lakes of Karelia and the Kola Peninsula as an independent species, Salvelinus lep- echini. The adaptation of the Arctic char to the unstable environmental conditions is ensured primarily by its phenotypic plasticity.

New information on the invasive to Europe aphid species Brachycaudus divaricatae Shaposhnikov, 1956 (Hemiptera: Aphididae)

Biologia ◽  
2012 ◽  
Vol 67 (5) ◽  
Author(s):  
Jekaterina Bašilova ◽  
Jan Havelka ◽  
Rimantas Rakauskas ◽  
Petr Starý ◽  
Jurga Turčinavičienė
Keyword(s):  
Czech Republic ◽  
Morphological Characters ◽  
Aphid Species ◽  
Coi Gene ◽  
Baltic Region ◽  
Mitochondrial Coi ◽  
The North ◽  
New Information ◽  
Two Samples ◽  
Eastern Baltic

AbstractBrachycaudus divaricatae (Hemiptera: Aphididae), a recent invader to Europe, has already reached Czech Republic. Partial sequences of mitochondrial COI and nuclear EF-1α genes have been analysed across the sixteen Czech samples of B. divaricatae, together with morphometric analysis of the same samples based on eighteen morphological characters of the apterous viviparous females. For comparative studies, thirteen samples from the Eastern Baltic region of Europe (Latvia, Lithuania and Poland) were used. All sampled populations appeared similar in their genetic and morphological characters studied. One haplotype of mitochondrial COI gene was predominant; it was characteristic for all samples from Czech Republic and 8 out of 13 samples from Eastern Baltic region. Two other haplotypes were found in the Eastern Baltic region only. Four different haplotypes of EF-1α gene were detected. Most of the samples (except one sample from the Eastern Baltic region and two samples from Czech Republic) had the same haplotype. Out of remaining three haplotypes, one was unique for the Eastern Baltic region, whilst two were found in Czech Republic only. For the present, Moravia is the southernmost region in Europe, where B. divaricatae has been already reported. Presumably, this invasive aphid species has entered the Czech Republic from the north via the Moravian Gate, a natural pass formed by the depression between the Western Carpathians and Eastern Sudetes.

scholarly journals Cryptic Patterns of Speciation in Cryptic Primates: Microendemic Mouse Lemurs and the Multispecies Coalescent

2020 ◽  
Author(s):  
Jelmer W Poelstra ◽  
Jordi Salmona ◽  
George P Tiley ◽  
Dominik Schüßler ◽  
Marina B Blanco ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Isolation By Distance ◽  
Divergence Time ◽  
Taxonomic Revision ◽  
Mouse Lemur ◽  
Effective Population ◽  
Separate Species ◽  
Divergence Time Estimates ◽  
Multispecies Coalescent ◽  
Mouse Lemurs

Abstract Mouse lemurs (Microcebus) are a radiation of morphologically cryptic primates distributed throughout Madagascar for which the number of recognized species has exploded in the past two decades. This taxonomic revision has prompted understandable concern that there has been substantial oversplitting in the mouse lemur clade. Here, we investigate mouse lemur diversity in a region in northeastern Madagascar with high levels of microendemism and predicted habitat loss. We analyzed RADseq data with multispecies coalescent (MSC) species delimitation methods for two pairs of sister lineages that include three named species and an undescribed lineage previously identified to have divergent mtDNA. Marked differences in effective population sizes, levels of gene flow, patterns of isolation-by-distance, and species delimitation results were found among the two pairs of lineages. Whereas all tests support the recognition of the presently undescribed lineage as a separate species, the species-level distinction of two previously described species, M. mittermeieri and M. lehilahytsara is not supported—a result that is particularly striking when using the genealogical discordance index (gdi). Nonsister lineages occur sympatrically in two of the localities sampled for this study, despite an estimated divergence time of less than 1 Ma. This suggests rapid evolution of reproductive isolation in the focal lineages and in the mouse lemur clade generally. The divergence time estimates reported here are based on the MSC calibrated with pedigree-based mutation rates and are considerably more recent than previously published fossil-calibrated relaxed-clock estimates. We discuss the possible explanations for this discrepancy, noting that there are theoretical justifications for preferring the MSC estimates in this case. [Cryptic species; effective population size; microendemism; multispecies coalescent; speciation; species delimitation.]

scholarly journals The discovery of Scutovertex ianus sp. nov. (Acari, Oribatida) – a combined approach of comparative morphology, morphometry and molecular data

2010 ◽  
Vol 79 (1) ◽  
pp. 39-55
Author(s):  
Tobias Pfingstl ◽  
Sylvia Schäffer ◽  
Ernst Ebermann ◽  
Günther Krisper
Keyword(s):  
New Species ◽  
Oribatid Mite ◽  
Intraspecific Variability ◽  
Comparative Morphology ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
Separate Species ◽  
Mitochondrial Coi ◽  
Molecular Phylogenetic

Based on morphological, morphometric and genetic data Scutovertex ianus sp. nov. is described as a new oribatid mite species. The traditional comparison with the morphologically most similar congeneric S. minutus and S. sculptus demonstrated that the new species shares certain characters with both species, but can be clearly identified by indistinct cuticular notogastral foveae in combination with short spiniform notogastral setae. Furthermore the eggs of S. ianus exhibit a different fine structure of the exochorion. The morphometric analysis of 16 continuous morphological variables separated the three species, S. minutus, S. sculptus and S. ianus with a certain overlap indicating minor size and shape differences in overall morphology. The molecular phylogenetic analysis of mitochondrial COI gene sequences supported the monophyly of all three investigated species and confirmed S. ianus as separate species with high bootstrap values. Each performed analysis approves the discreteness of S. ianus and the results contradict the formerly supposed large intraspecific variability of the representatives of the genus Scutovertex. The records of S. ianus are as yet restricted to the Eastern part of Austria and to one location in Germany, but findings of intermediary Scutovertex specimens from other European countries may refer to this new species.

scholarly journals First report of mitochondrial COI in foraminifera and implications for DNA barcoding

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jan-Niklas Macher ◽  
Jeremy G. Wideman ◽  
Elsa B. Girard ◽  
Anouk Langerak ◽  
Elza Duijm ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
18S Rrna ◽  
Species Recognition ◽  
Morphological Characters ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Foraminiferal Species ◽  
Wide Range ◽  
Coi Sequences ◽  
Global Carbon

AbstractForaminifera are a species-rich phylum of rhizarian protists that are highly abundant in many marine environments and play a major role in global carbon cycling. Species recognition in Foraminifera is mainly based on morphological characters and nuclear 18S ribosomal RNA barcoding. The 18S rRNA contains variable sequence regions that allow for the identification of most foraminiferal species. Still, some species show limited variability, while others contain high levels of intragenomic polymorphisms, thereby complicating species identification. The use of additional, easily obtainable molecular markers other than 18S rRNA will enable more detailed investigation of evolutionary history, population genetics and speciation in Foraminifera. Here we present the first mitochondrial cytochrome c oxidase subunit 1 (COI) gene sequences (“barcodes”) of Foraminifera. We applied shotgun sequencing to single foraminiferal specimens, assembled COI, and developed primers that allow amplification of COI in a wide range of foraminiferal species. We obtained COI sequences of 49 specimens from 17 species from the orders Rotaliida and Miliolida. Phylogenetic analysis showed that the COI tree is largely congruent with previously published 18S rRNA phylogenies. Furthermore, species delimitation with ASAP and ABGD algorithms showed that foraminiferal species can be identified based on COI barcodes.

Angiostrongylus vasorum from South America and Europe represent distinct lineages

Parasitology ◽  
2009 ◽  
Vol 136 (1) ◽  
pp. 107-115 ◽  
Author(s):  
R. JEFFERIES ◽  
S. E. SHAW ◽  
M. E. VINEY ◽  
E. R. MORGAN
Keyword(s):  
South America ◽  
Taxonomic Revision ◽  
Single Species ◽  
Angiostrongylus Vasorum ◽  
Coi Gene ◽  
Separate Species ◽  
Domestic Species ◽  
Mitochondrial Coi ◽  
Rates Of Evolution ◽  
Evolutionary Event

SUMMARYAngiostrongylus vasorum is a nematode parasite of sylvan and domestic species of the family Canidae. It has a broad but patchy distribution worldwide, and there is evidence for geographical spread and increasing incidence of infection in recent years. While historically Angiostrongylus-like nematodes identified in dogs and foxes have been described as A. vasorum in Europe and Angiocaulus raillieti in South America, more recent taxonomic revision has amalgamated these into a single species, A. vasorum. Here we report, for the first time, the molecular characterization of isolates of A. vasorum from Germany, Portugal, Denmark and the United Kingdom on the basis of the mitochondrial COI gene and the second ribosomal internal transcribed spacer. When compared with isolates from Brazil, sequence analysis revealed 2 distinct genotypes. Estimated rates of evolution based on COI sequences for both nematode and host are consistent with the hypothesis that the presence of A. vasorum in South America is a result of an ancient evolutionary event. Angiostrongylus vasorum in South America potentially represents a separate species to that observed in Europe.

scholarly journals Cryptic Patterns of Speciation in Cryptic Primates: Microendemic Mouse Lemurs and the Multispecies Coalescent

2019 ◽  
Author(s):  
Jelmer Poelstra ◽  
Jordi Salmona ◽  
George P. Tiley ◽  
Dominik Schüßler ◽  
Marina B. Blanco ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Isolation By Distance ◽  
Divergence Time ◽  
Mouse Lemur ◽  
Integrative Approach ◽  
Effective Population ◽  
Separate Species ◽  
Divergence Time Estimates ◽  
Multispecies Coalescent ◽  
Mouse Lemurs

AbstractMouse lemurs (Microcebus) are a radiation of morphologically cryptic primates distributed throughout Madagascar for which the number of recognized species has exploded in the past two decades. This taxonomic explosion has prompted understandable concern that there has been substantial oversplitting in the mouse lemur clade. Here, we take an integrative approach to investigate species diversity in two pairs of sister lineages that occur in a region in northeastern Madagascar with high levels of microendemism and predicted habitat loss. We analyzed RADseq data with multispecies coalescent (MSC) species delimitation methods for three named species and an undescribed lineage previously identified to have divergent mtDNA. Marked differences in effective population sizes, levels of gene flow, patterns of isolation-by-distance, and species delimitation results were found among them. Whereas all tests support the recognition of the presently undescribed lineage as a separate species, the species-level distinction of two previously described species, M. mittermeieri and M. lehilahytsara is not supported – a result that is particularly striking when using the genealogical discordance index (gdi). Non-sister lineages occur sympatrically in two of the localities sampled for this study, despite an estimated divergence time of less than 1 Ma. This suggests rapid evolution of reproductive isolation in the focal lineages, and in the mouse lemur clade generally. The divergence time estimates reported here are based on the MSC and calibrated with pedigree-based mutation rates and are considerably more recent than previously published fossil-calibrated concatenated likelihood estimates, however. We discuss the possible explanations for this discrepancy, noting that there are theoretical justifications for preferring the MSC estimates in this case.

scholarly journals Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Maysa Tiemi Motoki ◽  
Dina Madera Fonseca ◽  
Elliott Frederic Miot ◽  
Bruna Demari-Silva ◽  
Phoutmany Thammavong ◽  
...  
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
Aedes Albopictus ◽  
Isolation By Distance ◽  
Genetic Locus ◽  
Lao Pdr ◽  
Phylogenetic Network ◽  
Population Expansion ◽  
Recent Population Expansion ◽  
The Usa

Abstract Background The Asian tiger mosquito, Aedes (Stegomyia) albopictus (Skuse) is an important worldwide invasive species and can be a locally important vector of chikungunya, dengue and, potentially, Zika. This species is native to Southeast Asia where populations thrive in both temperate and tropical climates. A better understanding of the population structure of Ae. albopictus in Lao PDR is very important in order to support the implementation of strategies for diseases prevention and vector control. In the present study, we investigated the genetic variability of Ae. albopictus across a north-south transect in Lao PDR. Methods We used variability in a 1337-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1), to assess the population structure of Ae. albopictus in Lao PDR. For context, we also examined variability at the same genetic locus in samples of Ae. albopictus from Thailand, China, Taiwan, Japan, Singapore, Italy and the USA. Results We observed very high levels of genetic polymorphism with 46 novel haplotypes in Ae. albopictus from 9 localities in Lao PDR and Thailand populations. Significant differences were observed between the Luangnamtha population and other locations in Lao PDR. However, we found no evidence of isolation by distance. There was overall little genetic structure indicating ongoing and frequent gene flow among populations or a recent population expansion. Indeed, the neutrality test supported population expansion in Laotian Ae. albopictus and mismatch distribution analyses showed a lack of low frequency alleles, a pattern often seen in bottlenecked populations. When samples from Lao PDR were analyzed together with samples from Thailand, China, Taiwan, Japan, Singapore, Italy and the USA, phylogenetic network and Bayesian cluster analysis showed that most populations from tropical/subtropical regions are more genetically related to each other, than populations from temperate regions. Similarly, most populations from temperate regions are more genetically related to each other, than those from tropical/subtropical regions. Conclusions Aedes albopictus in Lao PDR are genetically related to populations from tropical/subtropical regions (i.e. Thailand, Singapore, and California and Texas in the USA). The extensive gene flow among locations in Lao PDR indicates that local control is undermined by repeated introductions from untreated sites.

Sign in / Sign up

Export Citation Format

Share Document