scholarly journals Characterization of relative biological effectiveness for conventional radiation therapy: a comparison of clinical 6 MV X-rays and 137Cs

2017 ◽  
Vol 58 (5) ◽  
pp. 608-613 ◽  
Author(s):  
Michelle Howard ◽  
Chris Beltran ◽  
Jann Sarkaria ◽  
Michael G Herman
Keyword(s):  
Radiation Therapy ◽  
Cell Lines ◽  
Relative Biological Effectiveness ◽  
Chinese Hamster ◽  
High Energy ◽  
X Rays ◽  
Human Glioma ◽  
Biological Damage ◽  
Biological Effectiveness ◽  
Lung Adenocarcinoma A549

ABSTRACT Various types of radiation are utilized in the treatment of cancer. Equal physical doses of different radiation types do not always result in the same amount of biological damage. In order to account for these differences, a scaling factor known as the relative biological effectiveness (RBE) can be used. 137Cesium (137Cs) has been used as a source of radiation in a significant body of radiation therapy research. However, high-energy X-rays, such as 6 MV X-rays, are currently used clinically to treat patients. To date, there is a gap in the literature regarding the RBE comparison of these two types of radiation. Therefore, the purpose of this study was to investigate the RBE of 137Cs relative to that of 6 MV X-rays. To determine the RBE, five cell lines were irradiated [Chinese hamster ovary (CHO); human lung adenocarcinoma (A549); human glioma (U251); human glioma (T98); and human osteosarcoma (U2OS)] by both types of radiation and assessed for cell survival using a clonogenic assay. Three of the five cell lines resulted in RBE values of ~1.00 to within 11% for all survival fractions, showing the physical and biological dose for these two types of radiation were equivalent. The other two cell lines gave RBE values differing from 1.00 by up to 36%. In conclusion, the results show the range in biological effect seen between cell lines, and therefore cell type must be considered when characterizing RBE.

scholarly journals High LET Radiation Overcomes In Vitro Resistance to X-Rays of Chondrosarcoma Cell Lines

2019 ◽  
Vol 18 ◽  
pp. 153303381987130
Author(s):  
Francois Chevalier ◽  
Dounia Houria Hamdi ◽  
Charlotte Lepleux ◽  
Mihaela Temelie ◽  
Anaïs Nicol ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Lines ◽  
Relative Biological Effectiveness ◽  
Radiation Treatment ◽  
Malignant Tumors ◽  
Treatment Options ◽  
X Rays ◽  
Chondrosarcoma Cell ◽  
Biological Effectiveness

Chondrosarcomas are malignant tumors of the cartilage that are chemoresistant and radioresistant to X-rays. This restricts the treatment options essential to surgery. In this study, we investigated the sensitivity of chondrosarcoma to X-rays and C-ions in vitro. The sensitivity of 4 chondrosarcoma cell lines (SW1353, CH2879, OUMS27, and L835) was determined by clonogenic survival assays and cell cycle progression. In addition, biomarkers of DNA damage responses were analyzed in the SW1353 cell line. Chondrosarcoma cells showed a heterogeneous sensitivity toward irradiation. Chondrosarcoma cell lines were more sensitive to C-ions exposure compared to X-rays. Using D10 values, the relative biological effectiveness of C-ions was higher (relative biological effectiveness = 5.5) with cells resistant to X-rays (CH2879) and lower (relative biological effectiveness = 3.7) with sensitive cells (L835). C-ions induced more G2 phase blockage and micronuclei in SW1353 cells as compared to X-rays with the same doses. Persistent unrepaired DNA damage was also higher following C-ions irradiation. These results indicate that chondrosarcoma cell lines displayed a heterogeneous response to conventional radiation treatment; however, treatment with C-ions irradiation was more efficient in killing chondrosarcoma cells, compared to X-rays.

scholarly journals Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vincent Paget ◽  
Mariam Ben Kacem ◽  
Morgane Dos Santos ◽  
Mohamed A. Benadjaoud ◽  
Frédéric Soysouvanh ◽  
...  
Keyword(s):  
Relative Biological Effectiveness ◽  
Clonogenic Assay ◽  
Umbilical Vein ◽  
High Energy ◽  
X Rays ◽  
X Ray ◽  
Biological Effectiveness ◽  
Umbilical Vein Endothelial Cells ◽  
Interventional Medicine

Abstract Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies are scarce. We therefore assessed in vitro the effects of low- and high-energy X-rays using Human umbilical vein endothelial cells (HUVECs) by performing clonogenic assay, measuring viability/mortality, counting γ-H2AX foci, studying cell proliferation and cellular senescence by flow cytometry and by performing gene analysis on custom arrays. Taken together, excepted for γ-H2AX foci counts, these experiments systematically show more adverse effects of high energy X-rays, while the relative biological effectiveness of photons is around 1, whatever the quality of the X-ray beam. These results strongly suggest that multiparametric analysis should be considered in support of clonogenic assay.

The Relative Biological Effectiveness of Low-Dose Mammography Quality X Rays in the Human Breast MCF-10A Cell Line

2015 ◽  
Vol 183 (1) ◽  
pp. 42-51 ◽  
Author(s):  
Caitlin E. Mills ◽  
Christopher Thome ◽  
David Koff ◽  
David W. Andrews ◽  
Douglas R. Boreham
Keyword(s):  
Cell Line ◽  
Low Dose ◽  
Relative Biological Effectiveness ◽  
X Rays ◽  
Human Breast ◽  
Biological Effectiveness

scholarly journals Investigating the impact of alpha/beta and LET d on relative biological effectiveness in scanned proton beams: An in vitro study based on human cell lines

2020 ◽  
Vol 47 (8) ◽  
pp. 3691-3702 ◽  
Author(s):  
Elisabeth Mara ◽  
Monika Clausen ◽  
Suphalak Khachonkham ◽  
Simon Deycmar ◽  
Clara Pessy ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Relative Biological Effectiveness ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Cell Lines ◽  
Biological Effectiveness ◽  
Alpha Beta ◽  
The Impact

Report of the AAPM TG-256 on the relative biological effectiveness of proton beams in radiation therapy

2019 ◽  
Vol 46 (3) ◽  
pp. e53-e78 ◽  
Author(s):  
Harald Paganetti ◽  
Eleanor Blakely ◽  
Alejandro Carabe-Fernandez ◽  
David J. Carlson ◽  
Indra J. Das ◽  
...  
Keyword(s):  
Radiation Therapy ◽  
Relative Biological Effectiveness ◽  
Proton Beams ◽  
Biological Effectiveness

scholarly journals A simple model for calculating relative biological effectiveness of X-rays and gamma radiation in cell survival

2020 ◽  
Vol 93 (1112) ◽  
pp. 20190949 ◽  
Author(s):  
Oleg N. Vassiliev ◽  
Christine B. Peterson ◽  
David R. Grosshans ◽  
Radhe Mohan
Keyword(s):  
Experimental Data ◽  
Cell Survival ◽  
Relative Biological Effectiveness ◽  
Survival Curve ◽  
Linear Functions ◽  
Model Parameters ◽  
High Dose ◽  
X Rays ◽  
Γ Radiation ◽  
Biological Effectiveness

Objectives: The relative biological effectiveness (RBE) of X-rays and γ radiation increases substantially with decreasing beam energy. This trend affects the efficacy of medical applications of this type of radiation. This study was designed to develop a model based on a survey of experimental data that can reliably predict this trend. Methods: In our model, parameters α and β of a cell survival curve are simple functions of the frequency-average linear energy transfer (LF) of delta electrons. The choice of these functions was guided by a microdosimetry-based model. We calculated LF by using an innovative algorithm in which LF is associated with only those electrons that reach a sensitive-to-radiation volume (SV) within the cell. We determined model parameters by fitting the model to 139 measured (α,β) pairs. Results: We tested nine versions of the model. The best agreement was achieved with [Formula: see text] and β being linear functions of [Formula: see text] .The estimated SV diameter was 0.1–1 µm. We also found that α, β, and the α/β ratio increased with increasing [Formula: see text] . Conclusions: By combining an innovative method for calculating [Formula: see text] with a microdosimetric model, we developed a model that is consistent with extensive experimental data involving photon energies from 0.27 keV to 1.25 MeV. Advances in knowledge: We have developed a photon RBE model applicable to an energy range from ultra-soft X-rays to megaelectron volt γ radiation, including high-dose levels where the RBE cannot be calculated as the ratio of α values. In this model, the ionization density represented by [Formula: see text] determines the RBE for a given photon spectrum.

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