GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis in Gladiolus hybridus

Dormant Arabidopsis (Arabidopsis thaliana) seeds do not germinate easily at temperatures higher than 10–15 °C. Using mutants affected in ethylene signaling (etr1, ein2 and ein4) and in the N-end-rule pathway of the proteolysis (prt6 and ate1-ate2) we have investigated the effects of cold and ethylene on dormancy alleviation. Ethylene (10–100 ppm) and 2–4 days chilling (4 °C) strongly stimulate the germination of wild type (Col-0) seeds at 25 °C. Two to four days of chilling promote the germination at 25 °C of all the mutants suggesting that release of dormancy by cold did not require ethylene and did not require the N-end-rule pathway. One mutant (etr1) that did not respond to ethylene did not respond to GA3 either. Mutants affected in the N-end rule (prt6 and ate1-ate2) did not respond to ethylene indicating that also this pathway is required for dormancy alleviation by ethylene; they germinated after chilling and in the presence of GA3. Cold can activate the ethylene signaling pathway since it induced an accumulation of ETR1, EINI4, and EIN2 transcripts, the expression of which was not affected by ethylene and GA3. Both cold followed by 10 h at 25 °C and ethylene downregulated the expression of PRT6, ATE1, ATE2, and of ABI5 involved in ABA signaling as compared to dormant seeds incubated at 25 °C. In opposite, the expression of RGA, GAI, and RGL2 encoding three DELLAs was induced at 4 °C but downregulated in the presence of ethylene.

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PpyGAST1 is potentially involved in bud dormancy release by integrating the GA biosynthesis and ABA signaling in ‘Suli’ pear (Pyrus pyrifolia White Pear Group)

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2019.03.008 ◽

2019 ◽

Vol 162 ◽

pp. 302-312 ◽

Cited By ~ 6

Author(s):

Qinsong Yang ◽

Qingfeng Niu ◽

Yinxin Tang ◽

Yunjing Ma ◽

Xinhui Yan ◽

...

Keyword(s):

Bud Dormancy ◽

Pyrus Pyrifolia ◽

Dormancy Release ◽

Aba Signaling ◽

Bud Dormancy Release ◽

Ga Biosynthesis

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Faculty Opinions recommendation of Arabidopsis KNOXI proteins activate cytokinin biosynthesis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1028591.342209 ◽

2005 ◽

Author(s):

Liam Dolan

Keyword(s):

Cytokinin Biosynthesis

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ABA signaling mediated by PYR/PYL/RCAR in plants

Hereditas (Beijing) ◽

10.3724/sp.j.1005.2012.00560 ◽

2012 ◽

Vol 34 (5) ◽

pp. 560-572 ◽

Cited By ~ 5

Author(s):

Shuai HU ◽

Fang-Zhan WANG ◽

Zhen-Ning LIU ◽

Ya-Pei LIU ◽

Xiao-Lin YU

Keyword(s):

Aba Signaling

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Expression Analysis of Genes Involved in Peanut Seed Dormancy Release (Arachis hypogaeaL.)

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2015.00845 ◽

2015 ◽

Vol 41 (6) ◽

pp. 845 ◽

Cited By ~ 1

Author(s):

Jing CHEN ◽

Ling JIANG ◽

Chun-Ming WANG ◽

Xiao-Hui HU ◽

Hu-Qu ZHAI ◽

...

Keyword(s):

Seed Dormancy ◽

Expression Analysis ◽

Dormancy Release ◽

Peanut Seed

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AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis

Scientific Reports ◽

10.1038/s41598-020-80695-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yanxing Ma ◽

Hainan Tian ◽

Rao Lin ◽

Wei Wang ◽

Na Zhang ◽

...

Keyword(s):

Stress Response ◽

Protein Phosphatase ◽

Aba Signaling ◽

Response Gene ◽

Stress Response Genes ◽

Arabidopsis Protein ◽

Evolutionarily Conserved ◽

Increased Sensitivity ◽

Aba Response ◽

Transcription Repressors

AbstractExpression of stress response genes can be regulated by abscisic acid (ABA) dependent and ABA independent pathways. Osmotic stresses promote ABA accumulation, therefore inducing the expression of stress response genes via ABA signaling. Whereas cold and heat stresses induce the expression of stress response genes via ABA independent pathway. ABA induced transcription repressors (AITRs) are a family of novel transcription factors that play a role in ABA signaling, and Drought response gene (DRG) has previously been shown to play a role in regulating plant response to drought and freezing stresses. We report here the identification of DRG as a novel transcription factor and a regulator of ABA response in Arabidopsis. We found that the expression of DRG was induced by ABA treatment. Homologs searching identified AITR5 as the most closely related Arabidopsis protein to DRG, and homologs of DRG, including the AITR-like (AITRL) proteins in bryophytes and gymnosperms, are specifically presented in embryophytes. Therefore we renamed DRG as AITRL. Protoplast transfection assays show that AITRL functioned as a transcription repressor. In seed germination and seedling greening assays, the aitrl mutants showed an increased sensitivity to ABA. By using qRT-PCR, we show that ABA responses of some ABA signaling component genes including some PYR1-likes (PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs) and SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASES 2s (SnRK2s) were reduced in the aitrl mutants. Taken together, our results suggest that AITRLs are a family of novel transcription repressors evolutionally conserved in embryophytes, and AITRL regulates ABA response in Arabidopsis by affecting ABA response of some ABA signaling component genes.

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Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2

Science Advances ◽

10.1126/sciadv.abd4113 ◽

2021 ◽

Vol 7 (12) ◽

pp. eabd4113

Author(s):

Rui Miao ◽

Wei Yuan ◽

Yue Wang ◽

Irene Garcia-Maquilon ◽

Xiaolin Dang ◽

...

Keyword(s):

Plasma Membrane ◽

Root Growth ◽

Experimental System ◽

Aba Signaling ◽

Wild Type ◽

Normal Medium ◽

C Terminus ◽

Quadruple Mutant ◽

Aba Insensitive ◽

Aba Receptors

The hab1-1abi1-2abi2-2pp2ca-1 quadruple mutant (Qabi2-2) seedlings lacking key negative regulators of ABA signaling, namely, clade A protein phosphatases type 2C (PP2Cs), show more apoplastic H+ efflux in roots and display an enhanced root growth under normal medium or water stress medium compared to the wild type. The presence of low ABA concentration (0.1 micromolar), inhibiting PP2C activity via monomeric ABA receptors, enhances root apoplastic H+ efflux and growth of the wild type, resembling the Qabi2-2 phenotype in normal medium. Qabi2-2 seedlings also demonstrate increased hydrotropism compared to the wild type in obliquely-oriented hydrotropic experimental system, and asymmetric H+ efflux in root elongation zone is crucial for root hydrotropism. Moreover, we reveal that Arabidopsis ABA-insensitive 1, a key PP2C in ABA signaling, interacts directly with the C terminus of Arabidopsis plasma membrane H+-dependent adenosine triphosphatase 2 (AHA2) and dephosphorylates its penultimate threonine residue (Thr947), whose dephosphorylation negatively regulates AHA2.

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Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00195-2 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Sridevi Muppala ◽

Pavan Kumar Gudlavalleti ◽

Kodandarami Reddy Malireddy ◽

Sateesh Kumar Puligundla ◽

Premalatha Dasari

Keyword(s):

Abiotic Stress ◽

Inbred Line ◽

Biochemical Characterization ◽

Abiotic Stress Tolerance ◽

Crop Improvement ◽

Maximum Frequency ◽

Aba Signaling ◽

Tolerant Plants ◽

Maize Plants ◽

Gateway Technology

Abstract Background In crop plants, to cope up with the demand of food for rising population, revolutionary crop improvement programmes are being implemented for higher and higher yields. Abiotic stress, especially at flowering stage, causes drastic effect on yield in plants. Deforestation and urbanization made the water table very low and changed the climate which led to untimely and unforeseen rains which affect the yield of a crop through stress, both by lack of water as well as water logging (abiotic stress). Development of tolerant plants through breeding is a time-consuming programme and does not perform well in normal conditions. Development of stress-tolerant plants through transgenic technology is the better solution. Maize is a major crop used as food and fodder and has the commercial value in ethanol production. Hence, the genes viz., nced (9-cis-epoxycarotenoid dioxygenase) and rpk (receptor-like protein kinase), which play the key roles in the abscisic acid pathway and upstream component in ABA signaling have been transferred into maize plants through Agrobacterium-mediated transformation by optimizing several parameters to obtain maximum frequency of transformation. Results Cultures raised from immature embryos of 2-mm size isolated from maize cobs, 12–15 days after pollination, were used for transformation. rpk and nced genes under the control of leaP and salT promoters respectively, cloned using gateway technology, have been introduced into elite maize inbred lines. Maximum frequency of transformation was observed with the callus infected after 20 days of inoculation by using 100 μM acetosyringone, 10 min infection time, and 2 days incubation period after co-cultivation resulted in maximum frequency of transformation (6%) in the NM5884 inbred line. Integration of the genes has been confirmed with molecular characterization by performing PCRs with marker as well as gene-specific primers and through southern hybridization. Physiological and biochemical characterization was done in vitro (artificial stress) and in vivo (pot experiments). Conclusions Changes in the parameters which affect the transformation frequency yielded maximum frequency of transformation with 20-day-old callus in the NM5884 inbred line. Introducing two or more genes using gateway technology is useful for developing stable transgenic plants with desired characters, abiotic stress tolerance in this study.

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