scholarly journals 133. The Changing Phenotypes and Genotypes of Invasive Pneumococcal Isolates From Children in Shenzhen During 2013–2017

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S95-S95
Author(s):  
Yanmin Bao ◽  
Yuejie Zheng
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
The Other ◽  
Significant Difference ◽  
Ermb Gene ◽  
Resistance To Penicillin ◽  
Resistance Rates ◽  
Sequence Types

Abstract Background The phenotypes and genotypes of Streptococcus pneumoniae (Spn.) isolated from children with invasive pneumococcal diseases (IPDs) were changed in these years. The purpose of this study was to monitor this mutation trends before the introduction of the 13-valent pneumococcal conjugate vaccine (PCV13) in China. Methods Strains were isolated from children less than 14 years old between January 2013 and May 2017 in Shenzhen Children’s Hospital. Serotypes, antibiotic resistance, and genotypes of these isolates were determined using capsular swelling, E-test, and multi-locus sequence typing, respectively. Results A total of 94 Spn. strains were isolated, which belonged to 15 serotypes. The five most prevalent serotypes were 19F (25.5%), 19A (19%), 14 (17%), 23F (7.5%), and 6B (9.6%). The other two serotypes (19C and 15) were non-vaccine types. We found 42 sequence types (STs) for these isolates. The most abundant STs were ST271 (24.4%), ST876 (17%), and ST320 (10.6%), mainly related to 19F, 14, and 19A, respectively. The potential coverage of PCV13 was 87.2%. Among non-meningitis isolates, the resistance rates to penicillin and ceftriaxone were 0% and 2%. However, the meningitis isolates showed universal resistance to penicillin (80%) and ceftriaxone (20%). Most of these isolates (95.7%) were resistant to erythromycin, and 66 (70.2%) strains carried the ermB gene and 24 (25.5%) strains carried both the ermB and mefA/E genes. Serotype 19A showed the highest minimum inhibitory concentration (MIC) for penicillin than the other serotypes, but no significant difference in penicillin MIC among the three main STs (ST271, ST320, and ST876). Conclusion The phenotypes and genotypes of invasive pneumococcal isolates from children in Shenzhen have changed with the passage of time. Compared with PCV7, PCV13 can more effectively protect Chinese children from IPDs. To some extent, these changes are possibly related to the usage of antibiotics and vaccines. Disclosures All authors: No reported disclosures.

Treatment of Acute Gonococcal Infections in Bangkok with a Dose Range of the New Cephalosporin, Cefodizime

1992 ◽  
Vol 3 (2) ◽  
pp. 111-116 ◽  
Author(s):  
A Chitwarakorn ◽  
C Wongba ◽  
E E Dagrosa ◽  
B Schinzel
Keyword(s):  
Antibiotic Resistance ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Dose Range ◽  
Geographic Region ◽  
Drug Reactions ◽  
Cure Rate ◽  
Intramuscular Dose ◽  
Resistance To Penicillin ◽  
Range Study

In this randomized dose range study conducted in Bangkok, 326 patients with presumed acute uncomplicated gonorrhoea were treated with a single intramuscular dose of either 0.25 g, 0.5 g, or 1.0 g of a new aminothiazole cephalosporin, cefodizime. One hundred and eighty men and 110 women were evaluable for efficacy. Pathogens were eliminated in all but 4 of these patients resulting in a cure rate of 99%, irrespective of dose, sex, or multiplicity of infected sites. A high percentage of the 290 strains of Neisseria gonorrhoeae isolated from the evaluable patients showed resistance to penicillin, and 40% were penicillinase producing (PPNG). The minimum inhibitory concentration of cefodizime for 90% of the PPNG strains was <0.04 mg/l. No clinically relevant adverse drug reactions were reported. This study demonstrates that a single 0.25 g dose of cefodizime is effective against both PPNG and non-PPNG strains in a geographic region characterized by high endemic antibiotic resistance.

scholarly journals Antibiotic Susceptibility of Staphylococcus aureus and Streptococcus pneumoniae Isolates from the Nasopharynx of Febrile Children under 5 Years in Nanoro, Burkina Faso

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 444
Author(s):  
Massa dit Achille Bonko ◽  
Palpouguini Lompo ◽  
Marc Christian Tahita ◽  
Francois Kiemde ◽  
Ibrahima Karama ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Burkina Faso ◽  
Antibiotic Susceptibility ◽  
Bacterial Colonization ◽  
Resistance To Penicillin ◽  
Tract Infections ◽  
Resistance Rates ◽  
Moderately Resistant ◽  
Children Under 5

(1) Background: nasopharynx colonization by resistant Staphylococcus aureus and Streptococcus pneumoniae can lead to serious diseases. Emerging resistance to antibiotics commonly used to treat infections due to these pathogens poses a serious threat to the health system. The present study aimed to determine the antibiotic susceptibility of S. aureus and S. pneumoniae isolates from the febrile children’s nasopharynx under 5 years in Nanoro (Burkina Faso). (2) Methods: bacterial isolates were identified from nasopharyngeal swabs prospectively collected from 629 febrile children. Antibiotic susceptibility of S. aureus and S. pneumoniae isolates was assessed by Kirby–Bauer method and results were interpreted according to the Clinical and Laboratory Standard Institute guidelines. (3) Results: bacterial colonization was confirmed in 154 (24.5%) of children of whom 96.1% carried S. aureus, 3.2% had S. pneumoniae, and 0.6% carried both bacteria. S. aureus isolates showed alarming resistance to penicillin (96.0%) and S. pneumoniae was highly resistant to tetracycline (100%) and trimethoprim–sulfamethoxazole (83.3%), and moderately resistant to penicillin (50.0%). Furthermore, 4.0% of S. aureus identified were methicillin resistant. (4) Conclusion: this study showed concerning resistance rates to antibiotics to treat suspected bacterial respiratory tract infections. The work highlights the necessity to implement continuous antibiotic resistance surveillance.

scholarly journals Macrolide-resistant phenotypes of invasive streptococcus pneumoniae isolates in Serbia

2012 ◽  
Vol 64 (4) ◽  
pp. 1377-1382
Author(s):  
Ina Gajic ◽  
Natasa Opavski ◽  
Vera Mijac ◽  
L. Ranin
Keyword(s):  
Streptococcus Pneumoniae ◽  
Susceptibility Testing ◽  
Reference Laboratory ◽  
National Reference ◽  
The Past ◽  
Triple Test ◽  
Resistance To Penicillin ◽  
Resistant Strains ◽  
Resistance Rates ◽  
Susceptibility Patterns

Macrolide resistance in Streptococcus pneumoniae has emerged as an important worldwide problem over the past decade. The aim of this study was to investigate macrolide-resistant phenotypes and the antimicrobial susceptibility patterns of invasive pneumococci in Serbia. A total of 68 invasive pneumococcal strains, collected from 2009 to 2011, were sent from regional laboratories to the National Reference Laboratory. Susceptibility testing was performed using the VITEK2 system and phenotypes were determined by triple-test. Overall penicillin and erythromycin nonsusceptibility rates were 26% and 43%, respectively. Resistance rates were higher in children than in adults. Co-resistance to penicillin and erythromycin was detected in 18% strains. Resistance rates to the third generation of cephalosporins, TMP-SXT and tetracycline were 16%, 37% and 29%, respectively. All isolates were fully susceptible to vancomycin, linezolid, fluoroquinolones, telithromycin and rifampicin. Twenty-two isolates (79%) an expressed macrolide-lincosamide-streptogramin B (MLSB) resistance phenotype and M phenotype was found in 21% of macrolide resistant strains.

scholarly journals Bacteremic Pneumonia Caused by Penicillin-Resistant Pneumococci: Case Report and Review with a Canadian Perspective

1992 ◽  
Vol 3 (4) ◽  
pp. 185-188
Author(s):  
David R Burdge ◽  
Vincent C Woo ◽  
Patricia MA Ritchie
Keyword(s):  
Antibiotic Resistance ◽  
Case Report ◽  
Streptococcus Pneumoniae ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Penicillin Resistance ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Canadian Perspective ◽  
Bacteremic Pneumonia

A Canadian adult with bacteremic pneumonia caused by a relatively penicillin-resistant (minimal inhibitory concentration 0.25 μg/mL) Streptococcus pneumoniae is reported, and the published literature regarding penicillin-resistant pneumococci in Canada reviewed. Although penicillin resistance has been reported infrequently to date, this case emphasizes the need for routine antimicrobial sensitivity testing of all pneumococci isolated from normally sterile sites, and for ongoing systematic surveillance for penicillin and other antibiotic resistance in Canada.

scholarly journals Antibacterial and Hemolytic Activity of Crotalus triseriatus and Crotalus ravus Venom

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 281
Author(s):  
Adrian Zaragoza-Bastida ◽  
Saudy Consepcion Flores-Aguilar ◽  
Liliana Mireya Aguilar-Castro ◽  
Ana Lizet Morales-Ubaldo ◽  
Benjamín Valladares-Carranza ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Hemolytic Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
The Other ◽  
Medical Treatments ◽  
First Time

Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity; on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.

Pilot Study on non-tuberculous mycobacteria infections in Italy

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
L Fattorini ◽  
A Iacobino ◽  
F Giannoni ◽  
Keyword(s):  
Cystic Fibrosis ◽  
Minimum Inhibitory Concentration ◽  
Pilot Study ◽  
Inhibitory Concentration ◽  
Strategic Plan ◽  
Foreign Born ◽  
Old Patients ◽  
National Strategic Plan ◽  
Reported Data ◽  
Resistance Rates

Abstract Background The rise in non-tuberculous mycobacteria (NTM) diseases has been reported worldwide, but no major information is known for Italy. The purpose of this pilot study is to release nationwide data on distribution of NTM in our country. Methods In 2016, the Istituto Superiore di Sanità (National Institute of Health) began to collect microbiological, clinical and minimum inhibitory concentration (MIC) data on NTM infections, in collaboration with hospital laboratories located in 15 out of 20 regions (Studio Multicentrico Italiano NTM, SMI-NTM), which routinely isolate and characterize NTM by commercial identification (Genotype) and MIC (Sensititre) assays. Results In 2016-2018, 32 labs reported data on 4169 NTM strains, including 644 rapid growers (15%) and 3525 slow growers (85%). The most frequent species were Mycobacterium avium (MA) (29.2%), M. intracellulare (MI) (21.5%), M. xenopi (MX) (10.6%), M. gordonae (10.6%), M. abscessus (5.9%), M. chimaera (MC) (5.1%). Overall, 88% NTM strains were isolated from pulmonary sites, 84% from Italians, 51% from men. NTM infections in Italians occurred in 75-84 years old patients, while in foreign-born people were observed in 15-44 years old patients. Strains from cystic fibrosis were 11.9%. The MICs of clarithromycin (CLA) for MA or MI peaked at 2 µg/ml, while for amikacin (AK) peaked at 16 µg/ml. When MICs were interpreted according to the 2018 Clinical and Laboratory Institute Standards (CLSI) breakpoints, MA or MI resistances for CLA were 2.6% and 2.6% respectively, while for AK they were 7.2% and 4.5%, respectively. Higher resistance rates for MA and MI were observed for moxifloxacin and linezolid. MICs of MC, MX, M. kansasii, M. marinum and rapid grower NTM were also determined and interpreted on the basis of CLSI breakpoints. Conclusions This 3-years pilot study is the basis for a future multiannual national strategic plan for surveillance of NTM infections in Italy (collection of 2019 data is in progress). Key messages This 3-years pilot study is the basis for a future multiannual national strategic plan for surveillance of NTM infections in Italy. The purpose of this pilot study is to release nationwide data on distribution of NTM in our country.

scholarly journals Characterization of Streptococcus pneumoniae isolates from India with special reference to their sequence types

2013 ◽  
Vol 7 (02) ◽  
pp. 101-109 ◽  
Author(s):  
Malini Shariff ◽  
Jyoti Choudhary ◽  
Shazia Zahoor ◽  
Monorama Deb
Keyword(s):  
Streptococcus Pneumoniae ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
The Elderly ◽  
New Delhi ◽  
Broth Microdilution ◽  
Mortality And Morbidity ◽  
Resistance To Penicillin ◽  
Sequence Types

Introduction: Streptococcus pneumoniae is a major cause of mortality and morbidity in young children and the elderly. In the present study we evaluated antimicrobial susceptibilities, serotypes, and sequence types of pneumococcal isolates recovered in New Delhi, India. Methodology: A total of 126 clinical isolates of Streptococcus pneumoniae were investigated. They were subjected to disk diffusion susceptibility testing, broth microdilution testing, serotyping and multilocus sequence typing. Results: Broth microdilution assay showed that 5%, 20% and 23% of the isolates exhibited resistance to penicillin, erythromycin and ciprofloxacin, respectively. Serotypes19, 1 and 6 were more frequently isolated. Thirty per cent of the strains were comprised of serotypes 1, 3, 5, 19A and 7F, which are not included in the seven-valent vaccine. Fifty-nine isolates were typed using multilocus sequence typing. Thirty new sequence types were encountered in this study. Only one clonal complex with 4 isolates was seen; 11 clonal complexes and 96 sequence types (STs) were observed among 115 Indian isolates. Only 18 of the 96 STs were found globally, of which only 4 STs were found in many countries with larger numbers. Conclusions: This study identifies the non-vaccine serotypes of Streptococcus pneumoniae circulating in India. It is important that an appropriate vaccine which covers all serotypes is used in the region.

scholarly journals Determination of antibiotic resistance and minimum inhibitory concentration of meropenem and imipenem growth in Klebsiella strains isolated from urinary tract infection in Shahrekord educational hospitals

2019 ◽  
Vol 21 (2) ◽  
pp. 80-85
Author(s):  
Farshad Kakian ◽  
Behnam Zamzad ◽  
Abolfazl Gholipour ◽  
Kiarash Zamanzad
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Minimum Inhibitory Concentration ◽  
Bacterial Infections ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Accurate Identification ◽  
Tract Infections

Background and aims: Klebsiella is an opportunistic organism that is the cause of severe diseases such as pneumonia, septicemia, and urinary tract infections (UTIs). In addition, high antibiotic resistance has challenged the treatment of this bacterium. However, carbapenem antibiotics are considered as the therapeutic agents for selecting the treatment of penicillin- and cephalosporin-resistant gram-negative bacterial infections. The present study aimed to determine the resistance and minimum inhibitory concentration (MIC) of meropenem and imipenem. Methods: A total of 80 Klebsiella spp isolated from UTIs were collected in various educational wards (i.e., urology, obstetrics, and gynecology, as well as the units of infectious diseases, internal medicine, and intensive care) in different hospitals of Shahrekord. The isolates were then identified by using biochemical tests. Further, disc diffusion method was employed to determine the antibiotic resistance. Furthermore, MIC was estimated by the Epsilon-test strip. Moreover, P=Q=0.50, an error of 0.05, and an accuracy of 0.11 were considered for determining the sample size (n=80). Results: Based on the results of disc diffusion method, 24 strains were resistant to meropenem and imipenem. Additionally, the MIC was 24 (30%) by the E-test. In addition, 24 isolates had a MIC of ≥4 μg/mL for meropenem and imipenem and thus were resistant while 18 isolates were found to have a MIC of 1≤ MIC<4 μg/mL and therefore, were considered semi-sensitive (P<0.001). Conclusion: In general, Klebsiella strains were found to be resistant to meropenem and imipenem. Therefore, rapid and accurate identification of these strains and the selection of appropriate antibiotics can help quickly eradicate the infections caused by these bacteria. Accordingly, a waste of time, the consumption of medication, or even an increased resistance are prevented.

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