scholarly journals GoldBricks: An Improved Cloning Strategy that Combines Features of Golden Gate and Biobricks for Better Efficiency and Usability

2021 ◽  
Author(s):  
Vishalsingh R Chaudhari ◽  
Maureen R Hanson
Keyword(s):  
Scale Up ◽  
Golden Gate ◽  
Slow Process ◽  
Library Size ◽  
Flexible Approach ◽  
User Input ◽  
Expression Studies ◽  
Cloning Strategy ◽  
Fast Pace ◽  
Speed Up

ABSTRACT With increasing complexity of expression studies and the repertoire of characterized sequences, combinatorial cloning has become a common necessity. Techniques like Biobricks and Golden Gate aim to standardize and speed up the process of cloning large constructs while enabling sharing of resources. The Biobricks format provides a simplified and flexible approach to endless assembly with a compact library and useful intermediates but is a slow process, joining only two parts in a cycle. Golden Gate improves upon the speed with use of TypeIIS enzymes and joins several parts in a cycle but requires a larger library of parts and logistical inefficiencies scale up significantly in the multigene format. We present here a method that provides improvement over these techniques by combining their features. By using Type IIS enzymes in a format like Biobricks, we have enabled a faster and efficient assembly with reduced scarring, which performs at a similarly fast pace as Golden Gate, but significantly reduces library size and user input. Additionally, this method enables faster assembly of operon-style constructs, a feature requiring extensive workaround in Golden Gate. Our format allows such inclusions resulting in faster and more efficient assembly.

An FPGA-based quantum circuit emulation framework using heisenberg representation

2018 ◽  
Vol 16 (06) ◽  
pp. 1850052
Author(s):  
Y. H. Lee ◽  
M. Khalil-Hani ◽  
M. N. Marsono
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Quantum Circuit ◽  
Theoretical Research ◽  
Resource Requirement ◽  
Heisenberg Representation ◽  
Speed Up ◽  
Computing Platforms ◽  
Classical Computing

While physical realization of practical large-scale quantum computers is still ongoing, theoretical research of quantum computing applications is facilitated on classical computing platforms through simulation and emulation methods. Nevertheless, the exponential increase in resource requirement with the increase in the number of qubits is an inherent issue in classical modeling of quantum systems. In the effort to alleviate the critical scalability issue in existing FPGA emulation works, a novel FPGA-based quantum circuit emulation framework based on Heisenberg representation is proposed in this paper. Unlike previous works that are restricted to the emulations of quantum circuits of small qubit sizes, the proposed FPGA emulation framework can scale-up to 120-qubit on Altera Stratix IV FPGA for the stabilizer circuit case study while providing notable speed-up over the equivalent simulation model.

scholarly journals Multilevel Gene Regulation Using Switchable Transcription Terminator and Toehold Switch in Escherichia coli

2021 ◽  
Vol 11 (10) ◽  
pp. 4532
Author(s):  
Seongho Hong ◽  
Jeongwon Kim ◽  
Jongmin Kim
Keyword(s):  
Escherichia Coli ◽  
Dynamic Range ◽  
Scale Up ◽  
Logic Gates ◽  
Living Cells ◽  
Genetic Circuit ◽  
Seamless Integration ◽  
Library Size ◽  
Transcription Terminator ◽  
Simultaneous Control

Nucleic acid-based regulatory components provide a promising toolbox for constructing synthetic biological circuits due to their design flexibility and seamless integration towards complex systems. In particular, small-transcriptional activating RNA (STAR) and toehold switch as regulators of transcription and translation steps have shown a large library size and a wide dynamic range, meeting the criteria to scale up genetic circuit construction. Still, there are limited attempts to integrate the heterogeneous regulatory components for multilevel regulatory circuits in living cells. In this work, inspired by the design principle of STAR, we designed several switchable transcription terminators starting from natural and synthetic terminators. These switchable terminators could be designed to respond to specific RNA triggers with minimal sequence constraints. When combined with toehold switches, the switchable terminators allow simultaneous control of transcription and translation processes to minimize leakage in Escherichia coli. Further, we demonstrated a set of logic gates implementing 2-input AND circuits and multiplexing capabilities to control two different output proteins. This study shows the potential of novel switchable terminator designs that can be computationally designed and seamlessly integrated with other regulatory components, promising to help scale up the complexity of synthetic gene circuits in living cells.

scholarly journals Stakeholder Perspectives in Transitioning to a Local Circular Economy: a Case Study in Spain

2021 ◽  
Author(s):  
J. Rincón-Moreno ◽  
M. Ormazábal ◽  
C. Jaca
Keyword(s):  
Circular Economy ◽  
Basque Country ◽  
Research Question ◽  
Scale Up ◽  
Local System ◽  
Closed Loop Systems ◽  
Stakeholder Perspectives ◽  
Circular Model ◽  
Speed Up

AbstractThe circular economy (CE) is now more imperative than ever due to several shortcomings humanity faces due to global economic disruptions. The CE might help initiate a virtuous circle whereby waste materials would be turned into resources for other companies to create closed-loop systems. However, the CE remains a niche paradigm embraced by only a small number of companies in some areas of the world. Some authors have argued that it is essential to encourage stakeholders at different implementation levels of a CE to increase the uptake of this new model among companies, and stakeholders may provide the required framework for the shift towards a circular model. Therefore, the purpose of this study is to answer the following research question: how can the transition towards the CE be accelerated from a ‘stakeholders’ perspective? The study was undertaken in the Spanish region of Basque Country, where a CE strategy is already in place at the regional level, and some companies are already delivering circular solutions. This approach was selected to help understand the lack of widespread adoption of CE initiatives in a local system despite having a regional CE strategy. Data were collected from respondents from various stakeholders and also from desk research to ensure their reliability. The results showed the relevance of understanding how stakeholders can help speed up the transition by proactively exploring new ways to create novel conditions to work together with a broad pool of stakeholders to deepen and scale up the CE implementation.

Policy brief on improving access to artemisinin-based combination therapies for malaria in the East African community

2010 ◽  
Vol 26 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Harriet Nabudere ◽  
Gabriel L. Upunda ◽  
Malick Juma
Keyword(s):  
Scale Up ◽  
World Health ◽  
Combination Therapies ◽  
East African ◽  
African Region ◽  
East African Community ◽  
African Community ◽  
Speed Up ◽  
Health Organization ◽  
Evidence Based Guidelines

The World Health Organization (WHO) since June 1998 has advocated for the use of artemisinin-based combination therapies (ACTs) in countries where Plasmodium falciparum malaria is resistant to traditional antimalarial therapies such as chloroquine, sulfadoxine-pyrimethamine, and amodiaquine (19;22). In 2006, WHO released evidence-based guidelines for the treatment of malaria backed by findings from various scientific studies (21). During the period between 2002 and 2006, all the five East African states Tanzania, Kenya, Uganda, Rwanda, and Burundi changed their national antimalarial treatment policies to use ACTs as first-line treatments for uncomplicated falciparum malaria and commenced with deployment of the drugs in the state-managed health facilities (12–15). To scale up the use of ACTs in the East African region to combat malaria and speed up progress toward the sixth Millennium Development Goal, a combination of delivery, financial, and governance arrangements tailored to national or subnational contexts needs to be considered.

scholarly journals A METADATA BASED APPROACH FOR ANALYZING UAV DATASETS FOR PHOTOGRAMMETRIC APPLICATIONS

2018 ◽  
Vol XLII-2 ◽  
pp. 297-302 ◽  
Author(s):  
A. Dhanda ◽  
F. Remondino ◽  
M. Santana Quintero
Keyword(s):  
Unmanned Aerial Vehicle ◽  
Flight Path ◽  
Large Block ◽  
User Input ◽  
Flight Plan ◽  
Aerial Vehicle ◽  
Speed Up ◽  
Block Sizes ◽  
Grid Based

This paper proposes a methodology for pre-processing and analysing Unmanned Aerial Vehicle (UAV) datasets before photogrammetric processing. In cases where images are gathered without a detailed flight plan and at regular acquisition intervals the datasets can be quite large and be time consuming to process. This paper proposes a method to calculate the image overlap and filter out images to reduce large block sizes and speed up photogrammetric processing. The python-based algorithm that implements this methodology leverages the metadata in each image to determine the end and side overlap of grid-based UAV flights. Utilizing user input, the algorithm filters out images that are unneeded for photogrammetric processing. The result is an algorithm that can speed up photogrammetric processing and provide valuable information to the user about the flight path.

scholarly journals WoZ4U: An Open-Source Wizard-of-Oz Interface for Easy, Efficient and Robust HRI Experiments

2021 ◽  
Vol 8 ◽  
Author(s):  
Finn Rietz ◽  
Alexander Sutherland ◽  
Suna Bensch ◽  
Stefan Wermter ◽  
Thomas Hellström
Keyword(s):  
Vital Role ◽  
Human Robot Interaction ◽  
Graphical Interface ◽  
Robot Interaction ◽  
Wizard Of Oz ◽  
User Input ◽  
Simple Hypothesis ◽  
Individual Experiment ◽  
Speed Up ◽  
General Tool

Wizard-of-Oz experiments play a vital role in Human-Robot Interaction (HRI), as they allow for quick and simple hypothesis testing. Still, a publicly available general tool to conduct such experiments is currently not available in the research community, and researchers often develop and implement their own tools, customized for each individual experiment. Besides being inefficient in terms of programming efforts, this also makes it harder for non-technical researchers to conduct Wizard-of-Oz experiments. In this paper, we present a general and easy-to-use tool for the Pepper robot, one of the most commonly used robots in this context. While we provide the concrete interface for Pepper robots only, the system architecture is independent of the type of robot and can be adapted for other robots. A configuration file, which saves experiment-specific parameters, enables a quick setup for reproducible and repeatable Wizard-of-Oz experiments. A central server provides a graphical interface via a browser while handling the mapping of user input to actions on the robot. In our interface, keyboard shortcuts may be assigned to phrases, gestures, and composite behaviors to simplify and speed up control of the robot. The interface is lightweight and independent of the operating system. Our initial tests confirm that the system is functional, flexible, and easy to use. The interface, including source code, is made commonly available, and we hope that it will be useful for researchers with any background who want to conduct HRI experiments.

Massive Data Analysis Based MapReduce Structure on Hadoop System

2014 ◽  
Vol 981 ◽  
pp. 262-266
Author(s):  
Yi Qiao Xu
Keyword(s):  
Data Analysis ◽  
Clustering Algorithms ◽  
Scale Up ◽  
Massive Data ◽  
Big Data Mining ◽  
System 2 ◽  
Speed Up ◽  
Application Fields ◽  
Parallel Clustering ◽  
Hadoop System

Massive Data analysis is becoming increasingly prominent in a variety of application fields ranging from scientific studies to business researches. In this paper, we demonstrate the necessity and possibility of using MapReduce [1] module on Hadoop System [2]. Furthermore, we conducted MapReduce module to implement Clustering Algorithms [3] on our Hadoop System [4] and improved the efficiency of the Clustering Algorithms sharply. We showed how to design parallel clustering algorithms based on Hadoop System. Experiments by different size of data demonstrate that our purposed clustering algorithms have good performance on speed-up, scale-up and size-up. So, it is suitable for big data mining and analysis.

NiAl precipitation in Fe-12w/o Cr-5w/o Ni-4w/o Al

1983 ◽  
Vol 41 ◽  
pp. 202-203
Author(s):  
L.E. Murr ◽  
J.S. Dunning ◽  
S. Shankar
Keyword(s):  
Solid Solution ◽  
Stainless Steel ◽  
Stainless Steels ◽  
Alloy Composition ◽  
Chromium Content ◽  
Scale Up ◽  
Optical Metallography ◽  
Property Evaluation ◽  
310 Stainless Steel ◽  
Microstructural Studies

Aluminum additions to conventional 18Cr-8Ni austenitic stainless steel compositions impart excellent resistance to high sulfur environments. However, problems are typically encountered with aluminum additions above about 1% due to embrittlement caused by aluminum in solid solution and the precipitation of NiAl. Consequently, little use has been made of aluminum alloy additions to stainless steels for use in sulfur or H2S environments in the chemical industry, energy conversion or generation, and mineral processing, for example.A research program at the Albany Research Center has concentrated on the development of a wrought alloy composition with as low a chromium content as possible, with the idea of developing a low-chromium substitute for 310 stainless steel (25Cr-20Ni) which is often used in high-sulfur environments. On the basis of workability and microstructural studies involving optical metallography on 100g button ingots soaked at 700°C and air-cooled, a low-alloy composition Fe-12Cr-5Ni-4Al (in wt %) was selected for scale up and property evaluation.

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