scholarly journals PSVII-36 Late-Breaking Abstract: Protein concentrations in basal diets affect metabolizable energy of feed ingredients determined by difference procedure in pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 340-341
Author(s):  
Hansol Kim ◽  
Jung Yeol Sung ◽  
Beob G Kim

Abstract The objective was to investigate the influence of crude protein (CP) concentrations in the basal diet on the metabolizable energy (ME) of feed ingredients determined using the difference procedure in pigs. Twelve barrows (73.7 ± 5.5 kg body weight) individually housed in metabolism crates were used. A low-protein basal diet (LPBD, 7.4% CP) was composed of 97.5% corn as the sole energy source and a high-protein basal diet (HPBD, 14.3% CP) was composed of 78.0% corn and 19.6% soybean meal. Four additional diets were prepared by replacing 20% of the energy sources in the basal diets with full-fat soybean (FFSB, 37.7% CP) or soy protein isolate (SPI, 87.6% CP). The 6 experimental diets were fed to 12 pigs employing a replicated 6 × 4 incomplete Latin square design with 4 periods. Each period was consisted of 4 days of adaptation and 4 days of collection period and the marker-to-marker method was used for fecal collection. Urinary gross energy output was greater (243 vs. 176 kcal/d; P < 0.05) in the HPBD group compared with the LPBD group. While ME in the HPBD was greater (3,418 vs. 3,322 kcal/kg; P < 0.05) than in the LPBD, ME in FFSB diets (3,585 and 3,633 kcal/kg in the LPBD and HPBD group, respectively) and SPI diets (3,537 and 3,537 kcal/kg, respectively) were not different between the 2 basal diet groups. Metabolizable energy of the test ingredients was less (P < 0.05) in pigs fed the HPBD group (4,565 and 4,111 kcal/kg in FFSB and SPI, respectively) compared with pigs fed the LPBD group (4,756 and 4,517 kcal/kg in FFSB and SPI, respectively). In conclusion, as the protein in the basal diet increases, metabolizable energy in a test ingredient determined using the difference procedure decreases mainly due to greater urinary energy output in pigs.

1970 ◽  
Vol 37 (3) ◽  
pp. 481-491 ◽  
Author(s):  
F. J. Gordon ◽  
T. J. Forbes

SummaryEight lactating cows were used in a Latin square experiment, to study the associative effects of level of energy and protein intake on milk yield and composition. Four diets were used, supplying 80 and 120% of estimated energy requirements and 80 and 120% of estimated protein requirements. The level of energy intake significantly affected milk yield, milk energy output, percentage butterfat, ash and non-protein nitrogen. The level of protein intake only significantly affected milk energy output and the non-protein nitrogen content of the milk. Although only the interaction of the effects of energy and protein intake on the milk content of solidsnot-fat (SNF) and ash was significant, it was evident that the effect of each of these factors on milk yield or composition was related to the level of the other in the diet.Input-output relationships within each protein level were used to compute the response in milk energy output and bodyweight change to a change in energy intake. These showed a greater partitioning of additional energy toward milk energy output with the high than with the low protein level. Multiple regression analysis within each level of protein intake was used to partition energy intake between that used for maintenance, milk energy output and liveweight change. The results showed efficiencies of utilization of metabolizable energy for milk output of 63 and 50% on the high- and low-protein diets, respectively.Nitrogen balance data are presented.


2019 ◽  
Vol 3 (2) ◽  
pp. 662-675 ◽  
Author(s):  
Charmaine D Espinosa ◽  
Su A Lee ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that digestibility of amino acids (AA), gross energy (GE), acid hydrolyzed ether extract (AEE), and neutral detergent fiber (NDF), and values for metabolizable energy (ME) in low-oil distillers dried grains with solubles (DDGS) vary among suppliers. In Exp. 1, the apparent total tract digestibility (ATTD) of GE, AEE, and NDF, and concentration of ME were determined in eight sources of DDGS (sources A, B, C, D, E, G, H, and I). A corn-based basal diet and eight diets containing corn and each source of DDGS were fed to 72 barrows (initial body weight = 18.1 ± 1.3 kg) with eight pigs per diet. Feces and urine were collected for 5 d after 7 d of adaptation. The ME did not differ among the eight sources of DDGS with the exception that DDGS source E contained less (P < 0.05) ME than DDGS source D. The ATTD of GE did also not differ among the eight sources of DDGS, but ME and ATTD of GE in corn were greater (P < 0.05) than in the eight sources of DDGS. However, the ATTD of AEE in corn and the eight sources of DDGS was not different, but the ATTD of AEE in DDGS source E was greater (P < 0.05) than in DDGS source A. The ATTD of NDF in DDGS source D was also greater (P < 0.05) than in DDGS sources E, G, and H, but ATTD of NDF did not differ between corn and the eight sources of DDGS. In Exp. 2, standardized ileal digestibility (SID) of AA was determined in seven sources of DDGS (sources A, B, C, D, E, G, and H). Twenty-four barrows (initial body weight = 63.4 ± 3.4 kg) that had a T-cannula installed in the distal ileum were allotted to a two-period incomplete Latin square design with eight diets. Seven diets were formulated to contain each of the seven sources of DDGS and an N-free diet was also used. Ileal digesta were collected for 2 d after 5 d of adaptation. There were no differences between pigs fed DDGS sources A and B in SID of AA, and the SID of Lys, Met, and Trp did not differ among DDGS sources A, B, and E. However, SID of most indispensable and dispensable AA except Gly were greater (P < 0.05) in DDGS source B than in DDGS sources C, D, E, G, and H. In conclusion, variability in SID of AA, ATTD of NDF and AEE, and ME were observed among the sources of DDGS used in this experiment.


1987 ◽  
Vol 57 (3) ◽  
pp. 355-361 ◽  
Author(s):  
Kirsten Christensen ◽  
Grete Thorbek

1. Total methane excretion (CH4 in breath+flatus) was measured in two experiments with thirty-six castrated male pigs (Danish Landrace) during the growth period from 20 to 120 kg live weight (LW). In Expt A, twenty-eight pigs were fed on a commercial diet alternately at high (HFL; metabolizable energy (ME) 1234 (SE 41) kJ/kg LW0.75) or low (LFL; ME 784 (SE 31) kJ/kg LW0.75) feed levels in different weight classes. In Expt B, eight pigs were constantly fed on a semi-purified diet at HFL without (-oil) or with 90 g soya-bean oil/kg diet (foil) corresponding to daily intakes of ME of 1339 (SE 11) and 1413 (SE 8) kJ/kg LW0.75 respectively.2. CH4 excretion was measured during 24 h respiration trials in open-air-circulation chambers.3. About 1 litre CH4 was excreted per day at 20–25 kg LW increasing to a maximum of 12 litres at 120 kg LW, which corresponded to no more than 1.2% of dietary gross energy.4. In Expt A, CH4 excretion increased linearly with LW, while in Expt B the increase was linear until about 70 kg LW, when it reached a plateau. On average LFL reduced CH, excretion by 23% compared with HFL. When related to dry matter (DM) intake, however, the pigs on LFL excreted 3.1 litres CH4/kg dietary DM and those on HFL 2.5 litres CH4/kg dietary DM, the difference being significant (P < 0.05). In Expt B the inclusion of soya-bean oil in the basal diet (+oil) reduced CH, excretion by 26% compared with the diet without oil (-oil). The pigs receiving the basal diet excreted 5.2 litres CH4/kg DM and the pigs receiving soya-bean oil 4.3 litres CH4/kg DM, the difference being highly significant (P < 0.001). All differences between Expt A and B in CH4 excretion based on DM intake were highly significant (P < 0.001).5. The results are discussed in relation to gas production in ruminants, rats and humans. It is suggested that flatus production may not only be reduced by changing the composition of the dietary carbohydrates, but also by inclusion of a polyunsaturated oil in the diet of simple-stomached animals and humans.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 116-117
Author(s):  
Jinsu Hong ◽  
Jung Wook Lee ◽  
Dan Pettersson ◽  
Tofuko A Woyengo

Abstract Objective of the study was to determine the effect of supplementing a combination of phytase and multi-carbohydrase on standardized ileal digestibility (SID) of amino acid (AA) and net energy (NE) value of carinata meal for growing pigs. Ten ileal-cannulated barrows (initial body weight = 53.9 ± 4.76 kg) were fed 4 diets in a replicated 4 × 4 Latin square design with 2 additional columns to give 10 replicates per diet. Diets included a corn-soybean meal (SBM)-based basal diet, the basal diet with 25% carinata meal, basal diet with 25% carinata meal plus phytase at 2,000 FTU/kg and multi-carbohydrase at 0.2g/kg, and a nitrogen-free diet. The multi-carbohydrase supplied 4 units of xylanase, 10 units of β-glucanase, and 1000 units of pectinase per kilogram of diet. The ratio of corn to soybean meal and soybean oil in carinata meal- containing diets was identical to that in the corn-soybean meal based basal diet to allow calculation of AA and energy digestibility of carinata meal by the difference method. On a DM basis, carinata meal contained 50.2% CP, 0.88% ether extract, 15.37% ADF, 1.82% Lys, 0.96% Met, 1.89% Thr, and 0.64% Trp, respectively. The SID of Lys, Met, Thr, and Trp for carinata meal were 51.4%, 82.2%, 65.9%, and 85.9%, respectively. Supplementation of a combination of phytase and multi-carbohydrase did not affect the SID of AA for the corn-SBM-carinata meal-based diet, and for the carinata meal. However, supplementation of a combination of phytase and multi-carbohydrase improved (P = 0.015) the apparent total tract digestibility of gross energy, and DE and NE values for carinata meal by 9.4, 9.5, and 12.4% respectively. In conclusion, the enzymes used in the current study could be added in carinata meal-based diets for pigs to improve the energy value of the carinata meal.


1993 ◽  
Vol 57 (2) ◽  
pp. 227-236 ◽  
Author(s):  
S. J. Oosting ◽  
H. A. Boekholt ◽  
M. J. N. Los ◽  
C. P. Leffering

AbstractTwo experiments, experiment 1 with six steers in a 3 × 3 Latin-square design and experiment 2 with four wether sheep in a cross-over design, were conducted to study the effect of species and ammonia treatment on intake and utilization of the energy of untreated wheat straw. Treatments were: (1) untreated wheat straw offered ad libitum on top of a basal diet (B) consisting of hay (0·25) and grass pellets (0·75) (UWS), (2) ammoniated wheat straw offered ad libitum plus B (AWS) and (3) ammoniated wheat straw offered at a restricted level plus B (AWS-). B was offered as a maintenance diet for both species and AWS- was only studied in steers. Voluntary intake of AWS zvas higher than that of UWS. No significant differences emerged between whole rations UWS and AWS with regard to energy digestion (ED), energy metabolizability (ρ = metabolizable energy (ME) I gross energy (GE)) and losses of digestible energy (DE) in urine and methane (average 187 J/KJ DE), but the efficiency of utilization of ME for growth (kg) was significantly higher for AWS than for UWS. ED and ρ of the straw part of the ration was significantly higher for AWS than for UWS. AWS- and AWS did not differ significantly with regard to ED, ρ and DE losses in methane and urine. Steers had a higher intake per kg0·75 per day than wether sheep. Across species, digestible energy intake (DEI) of the whole ad libitum fed diets was related to live weight (M)0·946 (s.e. of exponent 0·0152). ED and ρ of the straw part of the rations did not differ significantly between species, but steers had a significantly higher ED and ρ of β than wether sheep. Steers excreted a significantly lower proportion of DE in urine and a significantly higher proportion of DE in methane than did wethers. Total energy losses in urine and methane, however, did not differ between species.


2014 ◽  
Vol 59 (No. 2) ◽  
pp. 69-83 ◽  
Author(s):  
D.W. Liu ◽  
J.J. Zang ◽  
L. Liu ◽  
N.W. Jaworski ◽  
Z.J. Fan ◽  
...  

Three experiments were conducted to compare raw corn, extruded corn, and dehulled-extruded corn in piglets&rsquo; feeding. In Experiment (Exp.) 1, 8 barrows (19.9 &plusmn; 0.6 kg) were fed experimental diets containing one out of three corn samples and a rice-soybean meal basal diet in a double 4 &times; 4 Latin square design to determine the digestible energy (DE) and metabolizable energy (ME) in the corn samples using the difference method. The DE content in extruded (14.29 MJ/kg) and dehulled-extruded (14.42 MJ/kg) corn was greater (P &lt; 0.05) than in raw corn (13.57 MJ/kg). In Exp. 2, 5 barrows (26.2 &plusmn; 1.3 kg) were fitted with ileal T-cannulas and used in a 5 &times; 5 Latin square design to determine the apparent (AID) and standardized (SID) ileal digestibility of amino acids (AA). The diets comprised the basal diet, the three corn diets from Exp. 1, as well as a nitrogen-free diet to estimate basal endogenous losses of AA. The AID and SID of isoleucine, leucine, lysine, threonine, and valine in dehulled-extruded corn was lower (P &lt; 0.05) than in raw or extruded corn. In Exp. 3, 108 weaned 35&nbsp;days old piglets (8.4 &plusmn; 1.2 kg) were allotted to one of the three diets based on corn type. Weaned pigs fed diets containing extruded or dehulled-extruded corn exhibited reduced (P &lt; 0.05) weight gain and feed intake than pigs fed diets containing raw corn. The diet containing dehulled-extruded corn resulted in a higher incidence of diarrhoea. In summary, extrusion of corn did not result in improvements in digestibility and dehulling corn prior to extrusion appeared to result in heat damage which reduced ileal digestibility of AA. Substitution of raw corn with extruded or dehulled-extruded corn in starter diets formulated to equal quantities of ME and SID AA content did not improve the performance of weaned pigs. &nbsp;


1994 ◽  
Vol 59 (2) ◽  
pp. 309-313 ◽  
Author(s):  
S. C. Cilliers ◽  
J. P. Hayes ◽  
J. S. Maritz ◽  
A. Chwalibog ◽  
J. J. du Preez

AbstractIn an experiment involving 30 roosters and 15 mature male ostriches the apparent metabolizable energy (ME) values, corrected for zero nitrogen retention (AMEn), were determined by balance method for maize and lucerne meal. A group of 10 roosters received maize as the sole dietary component. For the second group of 10 roosters a blend of 750 g/kg maize and 250 g/kg lucerne was prepared. The diet of the third group consisted of 500 g/kg maize and 500 g/kg lucerne meal. The ostrich basal diet consisted of 1000 g/kg lucerne, and was blended in the ratios 50 lucerne: 50 maize and 25 lucerne: 75 maize. Each dietary treatment was given to five ostriches, individually housed in metabolism crates. Food intake and excreta collection was carried out over a period of 5 days after the ostriches had been accustomed to the diets for 7 days. For roosters food intake and excreta collection lasted for 72 h after an adaptation period of 24 h.The AMEn value for maize was 14·49 (s.e. 0·046) MJ/kg in roosters while in ostriches AME,, values of 14·3 (s.e. 1·81) and 14·5 (s.e. 0·845) MJ/kg for the 500 g/kg inclusion and 250 g/kg inclusion respectively were found.In roosters lucerne meal yielded AME,, values of 4·49 (s.e. 0·506) and 4·05 (s.e. 0·321) at 250 g/kg and 500 g/kg inclusion levels respectively. For the ostriches a value of 8·9 (s.e. 0·755) Mj/kg was found for lucerne.True ME, corrected for zero nitrogen retention, (TMEJ was computed by regressing gross energy output on gross energy input over all feeding levels. Maize yielded values of 14·65 (s.e. 0·0455) and 14·9 (s.e. 0·351) for roosters and ostriches respectively while corresponding values for lucerne were 4·03 (s.e. 0·118) and 8·6 (s.e. 0·296) MJ/kg.It was concluded that the ostriches were capable of digesting a high starch diet such as maize to the same extent as adult roosters but ostriches were capable of digesting a high fibre ingredient such as lucerne meal much more efficiently.


Author(s):  
Alison N Beloshapka ◽  
Tzu-Wen L Cross ◽  
Kelly S Swanson

Abstract Resistant starch (RS) is fermentable by gut microbiota and effectively modulates fecal short-chain fatty acid concentrations in pigs, mice, and humans. RS may have similar beneficial effects on the canine gut, but has not been well studied. The objective of this study was to evaluate the effects of 0, 1, 2, 3, and 4% dietary RS (Hi-maize 260) on apparent total tract macronutrient digestibility, and fecal characteristics, fermentative end-product concentrations, and microbiota of healthy adult dogs. An incomplete 5 x 5 Latin square design with 7 dogs and 5 experimental periods was used, with each treatment period lasting 21 d (d 0-17 adaptation; d 18-21 fresh and total fecal collection) and each dog serving as its own control. Seven dogs (mean age = 5.3 yr; mean BW = 20 kg) were randomly allotted to one of five treatments formulated to be iso-energetic and consisting of graded amounts of 100% amylopectin cornstarch, RS, and cellulose, and fed as a top dressing on the food each day. All dogs were fed the same amount of a basal diet throughout the study and fresh water was offered ad libitum. The basal diet contained 6.25% RS (DM basis), contributing approximately 18.3 g of RS/d based on their daily food intake (292.5 g DM/d). Data were evaluated for linear and quadratic effects using SAS. The treatments included 0%, 1%, 2%, 3%, and 4% of an additional RS source. Because Hi-maize 260 is approximately 40% digestible and 60% indigestible starch, the dogs received the following amounts of RS daily: 0% = 18.3 g (18.3 g + 0 g); 1% = 20.1 g (18.3 g + 1.8 g); 2% = 21.9 g (18.3 g + 3.6 g); 3% = 23.7 g (18.3 g + 5.4 g); and 4% = 25.5 g (18.3 g + 7.2 g). Apparent total tract dry matter, organic matter, crude protein, fat, and gross energy digestibilities and fecal pH were linearly decreased (P &lt; 0.05) with increased RS consumption. Fecal output was linearly increased (P &lt; 0.05) with increased RS consumption. Fecal scores and fecal fermentative end-product concentrations were not affected by RS consumption. Although most fecal microbial taxa were not altered, Faecalibacterium were increased (P &lt; 0.05) with increased RS consumption. The decrease in fecal pH and increase in fecal Faecalibacterium would be viewed as being beneficial to gastrointestinal health. Although our results seem to indicate that RS is poorly and/or slowly fermentable in dogs, the lack of observed change may have been due to the rather high level of RS contained in the basal diet.


1972 ◽  
Vol 79 (1) ◽  
pp. 99-103 ◽  
Author(s):  
A. M. Raven

SUMMARYA 6 x 6 Latin Square balance experiment was carried out using six Friesian steers, each of which initially weighed about 304 kg. The six treatments studied were an all-hay diet and five other diets containing 20,40,60,80 and 100 % of rolled barley fortified with mineral and vitamin supplements, accompanied by correspondingly reduced proportions of hay. Each diet was fed at an estimated maintenance level of feeding.The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in both digestible and calculated metabolizable energy. The actual increase in digestible energy was from 2·62Mcal/kg dry matter (59·3% of the gross energy) on the all-hay treatment to 3·42 Mcal/kg dry matter (79·5% of the gross energy) on the all-concentrate treatment. Use of the determined digestible energy values for the all-hay and fortified barley diets to calculate the digestible energy of the four mixed diets gave results in reasonably good agreement with the determined values, the maximum difference being 0·12 Mcal/kg dry matter, which represented 3·83 % of the determined value. The losses of energy in the urine expressed as percentages of the gross energy of the diets showed a small but significantly linear decrease (P < 0·01) with increase in proportion of barley in the diet. The molar proportions of steamvolatile acids in samples of rumen fluid taken from two animals on each treatment indicated that increase in the proportion of concentrate was associated with tendencies for increase in acetic acid, decrease in propionic acid and little change in butyric acid. The mean digestibility of the organic matter was 62·6 % on the all-hay treatment and 81·8 % on the all concentrate treatment. The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in digestibility of the organic matter. Although intakes of nitrogen decreased with increase in the proportion of concentrate due to a decrease in the amount of dry matter fed, the weights of nitrogen retained were well maintained and when expressed as percentages of intake showed a significantly linear increase (P < 0·01).


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 394-395
Author(s):  
Jongkeon Kim ◽  
Yun Yeong Jo ◽  
Beob Gyun G Kim

Abstract The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in high-fiber ingredients fed to growing pigs. Twelve barrows with an initial body weight of 57.5 kg (SD = 5.7) were individually housed in metabolism crates. A replicated 6 × 3 incomplete Latin square design with 12 animals, 6 experimental diets and 3 periods was employed. A basal diet was composed of 75.0% corn and 22.7% soybean meal (SBM) as the sole energy sources. Four experimental diets were prepared by replacing 40% of corn and SBM with soybean hulls (SH), corn gluten feed (CGF), wheat bran (WB), or rice bran (RB). An additional diet was prepared by replacing 10% of corn and SBM with cashew nut hulls (CNH). Each period consisted of a 4-d adaptation period and a 4-d collection period, and the marker-to-marker procedure was used for total collection of feces and urine. The DE and ME values in RB (3,969 and 3,936 kcal/kg DM) were greater (P &lt; 0.05) than those in CGF (2,654 and 2,520 kcal/kg DM) and SH (2,492 and 2,541 kcal/kg DM) and the energy values in WB (3,162 and 3,118 kcal/kg DM) were not different from those in RB, CGF, or SH. The DE and ME values in CNH (350 and 572 kcal/kg DM) were less (P &lt; 0.05) than those in all other test ingredients. In conclusion, energy concentrations in RB were greatest among the high-fiber test ingredients, whereas CNH had the lowest values.


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