scholarly journals Endophytic microbiome variation among single plant seeds

2021 ◽  
Author(s):  
Ari Fina Bintarti ◽  
Abby Sulesky-Grieb ◽  
Nejc Stopnišek ◽  
Ashley Shade
Keyword(s):  
Common Bean ◽  
Phaseolus Vulgaris L ◽  
Plant Seeds ◽  
Individual Seed ◽  
Bean Plants ◽  
Plant Level ◽  
The Individual ◽  
Microbial Dna ◽  
Microbiome Assembly ◽  
Plant Microbiome

Like other plant compartments, the seed harbors a microbiome. Seed microbiome members are the first to colonize a germinating seedling, and they may initiate the trajectory of microbiome assembly for the next plant generation. Therefore, the members of the seed microbiome are important for the dynamics of plant microbiome assembly and the vertical transmission of potentially beneficial symbionts. However, it remains challenging to assess the microbiome at the individual seed level (and, therefore, for the future individual plants) due to low endophytic microbial biomass, seed exudates that can select for particular members, and high plant and plastid contamination of resulting reads. Here, we report a protocol for extracting microbial DNA from an individual seed (common bean, Phaseolus vulgaris L.) with minimal disruption of host tissue, which we expect to be generalizable to other medium- and large-seed plant species. We applied this protocol to determine the 16S rRNA V4 and rRNA ITS2 amplicon composition and examine the variability of individual seeds harvested from replicate common bean plants grown under standard, controlled conditions to maintain health. Using DNA extractions from individual seeds, we compared seed-to-seed, pod-to-pod, and plant-to-plant microbiomes, and found highest microbiome variability at the plant level. This suggests that several seeds from the same plant could be pooled for microbiome assessment, given experimental designs that apply treatments at the parent plant level. This study adds protocols and insights to the growing toolkit of approaches to understand the plant-microbiome engagements that support the health of agricultural and environmental ecosystems.

scholarly journals Endophytic microbiome variation at the level of a single plant seed

2021 ◽  
Author(s):  
AF Bintarti ◽  
A Sulesky-Grieb ◽  
N Stopnisek ◽  
A Shade
Keyword(s):  
Common Bean ◽  
Individual Plant ◽  
Metagenomic Dna ◽  
Plant Seed ◽  
Individual Seed ◽  
Bean Plants ◽  
Plant Level ◽  
The Individual ◽  
Microbiome Assembly ◽  
Plant Microbiome

AbstractLike other plant compartments, the seed harbors a microbiome. The members of the seed microbiome are the first to colonize a germinating seedling, and they initiate the trajectory of microbiome assembly for the next plant generation. Therefore, the members of the seed microbiome are important for the dynamics of plant microbiome assembly and the vertical transmission of potentially beneficial symbionts. However, it remains challenging to assess the microbiome at the individual seed level (and, therefore, for the future individual plant) due to low endophytic microbial biomass, seed exudates that can select for particular members, and high plant and plastid contamination of resulting reads. Here, we report a protocol for extracting metagenomic DNA from an individual seed (common bean, Phaseolus vulgaris L.) with minimal disruption of host tissue, which we expect to be generalizable to other medium-and large-seed plant species. We applied this protocol to quantify the 16S rRNA V4 and ITS2 amplicon composition and variability for individual seeds harvested from replicate common bean plants grown under standard, controlled conditions to maintain health. Using metagenomic DNA extractions from individual seeds, we compared seed-to-seed, pod-to-pod, and plant-to-plant microbiomes, and found highest microbiome variability at the plant level. This suggests that several seeds from the same plant could be pooled for microbiome assessment, given experimental designs that apply treatments at the maternal plant level. This study adds protocols and insights to the growing toolkit of approaches to understand the plant-microbiome engagements that support the health of agricultural and environmental ecosystems.

scholarly journals Growth and production of common bean fertilized with biochar

2017 ◽  
Vol 47 (11) ◽  
Author(s):  
Isley Cristiellem Bicalho da Silva ◽  
Luiz Arnaldo Fernandes ◽  
Fernando Colen ◽  
Regynaldo Arruda Sampaio
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Mass Number ◽  
Dry Mass ◽  
Organic Wastes ◽  
Phaseolus Vulgaris L ◽  
Bean Plants ◽  
Filtration Material ◽  
Sorghum Silage ◽  
Common Bean Plants

ABSTRACT: Production of biochar from organic wastes promises to be an interesting source of plant nutrients, thus reducing pressure on natural resources. To assess the effect of biochar prepared from wastes filtration materials on the growth and production of common bean (Phaseolus vulgaris L.), three simultaneous greenhouse experiments were conducted with three different biochar from organic wastes (rice husk, sawdust, and sorghum silage) using as filtration material for swine biofertilizer. In each experiment the treatments consisted of the addition of five different biochar concentrations (0%, 2.5%, 5%, 7.5%, and 10% v/v), arranged in a completely random design, with four repetitions. Application of biochar increased the root dry mass, shoot dry mass, grain dry mass, number of pods and number of grains. These results indicated that biochar contributed significantly to the growth and production of common bean plants.

scholarly journals Comparison of Violet, Red, and Green Light on Early-Stage Common Bean (Phaseolus vulgaris L.) Development

2020 ◽  
Vol 120 (2) ◽  
pp. 49
Author(s):  
Aaron Ziegler
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Early Stage ◽  
Red Light ◽  
Green Light ◽  
Phaseolus Vulgaris L ◽  
P Values ◽  
Violet Light ◽  
Bean Plants ◽  
Common Bean Plants

The energy efficiency of light-emitting diodes (LEDs) makes them attractive for indoor plant lighting. LEDs, however, do not produce broad-spectrum light efficiently. The effects of LED lighting on common bean (Phaseolus vulgaris L.) growth are not well known. This study sought to find the colors of light that most effectively grow common bean plants under indoor conditions. The hypothesis was that red light would promote early common bean growth better, both qualitatively and quantitatively, than violet or green light. After planting the seeds in soil, 20 common bean plants were each grown under red, or green, or violet LED lights (PHILIPS®) (8 watt), or natural sunlight, for 16 days: a total of 80 plants. Bonferroni adjusted t-tests showed that the plants under the violet light grew significantly taller than the plants under red or green light with p-values 0.000 respectively; the plants under red or green light had significantly larger leaves than those under the violet light with p-values 0.000. Qualitative observations (based upon visual inspections of leaf health, maturity, and root development) revealed the plants under red light were the healthiest, most mature, and exhibited the most developed roots—followed by those under green light. The findings of this study suggested early common bean growth performed better under red light than under violet or green light.

Impacts of Rhizobium Strain Ar02 on the Nodulation, Growth, Nitrogen (N2) Fixation Rate and ion Accumulation in Phaseolus vulgaris L. under Salt Stress

2021 ◽  
Author(s):  
Saoussen Kouki ◽  
Boulbaba L’taief ◽  
Rahamh N. Al-Qthanin ◽  
Bouaziz Sifi
Keyword(s):  
Salt Stress ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Phaseolus Vulgaris L ◽  
Fixation Rate ◽  
Bean Plants ◽  
Nodule Biomass ◽  
The Common ◽  
Saline Solutions ◽  
Common Bean Plants

Background: Phaseolus vulgaris L. -rhizobia symbiosis has effectively enhanced common bean productivity via multiple biological mechanisms. This study aims to assess the impacts of the strain of Rhizobium on the nodulation, growth, nitrogen (N2) fixation rate and ion accumulation within Phaseolus vulgaris L. under salt stress. Methods: The Coco Blanc cultivar of the common bean was inoculated with the Ar02 rhizobia strain at 15 days after germination. Bean plants were inoculated in perlite culture to which salt was added in concentrations of 0, 25, 50 and 75 mmol L-1 NaCl. Result: Inoculation with the Ar02 rhizobia strain led to infective and effective symbiosis with the common bean plants exposed to saline solutions and non-saline solutions, respectively. Nodule biomass and nitrogen content declined under salt stress but maintained a higher number of nodules and nodule biomass at 75 mM NaCl. Plant root and shoot length increased with higher biomass under saline conditions, significantly more than the non-inoculated plant without salt. However, the progressive addition of NaCl reduced the growth of the root and shoot and the biomass within the inoculated plant. Salinity led to increased Na+ within the plant’s shoot, along with a reduction in Ca+2 and K+ concentrations. The shoot’s Ca+2, Na+ and K+ content were higher in the inoculated plant than the non-inoculated. The salt tolerance in common bean plants inoculated with Ar02 rhizobia was linked with the plant’s capability to sustain nodulation and enhance Na+ concentration in the shoot. Furthermore, salt tolerance within the same variety inoculated with Rhizobium was linked to a decline in the Ca+ and K+ concentrations in the shoot region of salt-exposed plants.

scholarly journals Diversity of Common Bean Landraces Collected in the Western and Eastern Carpatien

2011 ◽  
Vol 39 (No. 3) ◽  
pp. 73-83 ◽  
Author(s):  
O. Horňáková ◽  
M. Závodná ◽  
M. Žáková ◽  
J. Kraic ◽  
F. Debre
Keyword(s):  
Cluster Analysis ◽  
Common Bean ◽  
Morphological Characters ◽  
Molecular Data ◽  
Seed Traits ◽  
Phaseolus Vulgaris L ◽  
Growth Type ◽  
Banding Patterns ◽  
Polymorphism Detection ◽  
Thousand Seed Weight

The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western andEastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics. Nevertheless, some of the accessions were identical in these characteristics. Cluster analysis grouped genotypes into two main branches, reflecting the growth type, seed size parameters, and thousand-seed weight. Molecular differentiation studies were performed by multilocus polymorphism detection in microsatellite and minisatellite DNA regions. Cluster analysis based on molecular data also grouped genotypes but no linkage to morphological traits was revealed. Bean accessions with very similar or identical morphological characters were clearly distinguished by DNA banding patterns. The presence of duplicates was excluded.  

scholarly journals Characterization of common bean ( Phaseolus vulgaris L.) germplasm for morphological and seed nutrient traits from Western Himalayas

2021 ◽  
Author(s):  
Sofora Jan ◽  
Irshad Ahmad Rather ◽  
Parvaze Ahmad Sofi ◽  
Mohd Altaf Wani ◽  
Farooq Ahmad Sheikh ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Western Himalayas ◽  
Phaseolus Vulgaris L ◽  
Nutrient Traits
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