Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya

The potyvirus Moroccan watermelon mosaic virus (MWMV) naturally infects and severely threatens production of cucurbits and papaya. In this study, we identified and characterized MWMV isolated from pumpkin (Cucurbita moschata) intercropped with MWMV-infected papaya plants through next generation and Sanger sequencing approaches. Complete MWMV genome sequences were obtained from two pumpkin samples through NGS and validated using Sanger sequencing. The isolates share 83.4-83.7 % nucleotide (nt) and 92.3-95.1 % amino acid (aa) sequence identities in the coat protein and 79.5-79.9 % nt and 89.2-89.7 % aa identities in the polyprotein with papaya isolates of MWMV. Phylogenetic analysis using complete polyprotein nt sequences revealed the clustering of both pumpkin isolates of MWMV with corresponding sequences of cucurbit isolates of the virus from other parts of Africa and the Mediterranean regions, distinct from a clade formed by papaya isolates. Through sap inoculation, a pumpkin isolate of MWMV was pathogenic on zucchini (Cucurbita pepo), watermelon (Citrullus lanatus), and cucumber (Cucumis sativus), but not on papaya. Conversely, the papaya isolate of MWMV was non-pathogenic on pumpkin, watermelon, and cucumber, but infected zucchini. The results suggest occurrence of two strains of MWMV in Kenya having different biological characteristics associated with the host specificity.

Download Full-text

Frequency and molecular characterization of watermelon mosaic virus from Serbia

Pesticidi i fitomedicina ◽

10.2298/pif1003213v ◽

2010 ◽

Vol 25 (3) ◽

pp. 213-230

Author(s):

Ana Vucurovic ◽

Aleksandra Bulajic ◽

Ivana Stankovic ◽

Danijela Ristic ◽

Janos Berenji ◽

...

Keyword(s):

Virus Infection ◽

Mosaic Virus ◽

Cucurbita Pepo ◽

Zucchini Yellow Mosaic Virus ◽

Growing Season ◽

Yellow Mosaic Virus ◽

Watermelon Mosaic Virus ◽

Mixed Infections ◽

Mediterranean Regions

Watermelon mosaic virus (WMV) is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group), as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELI

Download Full-text

First Report of Zucchini yellow mosaic virus in Cucumber (Cucumis sativus) in India

Plant Disease ◽

10.1094/pdis.2004.88.8.906b ◽

2004 ◽

Vol 88 (8) ◽

pp. 906-906 ◽

Cited By ~ 5

Author(s):

Raj Verma ◽

Satya Prakash ◽

S. P. S. Tomer

Keyword(s):

Mosaic Virus ◽

Cucumis Sativus ◽

Cucurbita Pepo ◽

Citrullus Lanatus ◽

Natural Infection ◽

Zucchini Yellow Mosaic Virus ◽

Yellow Mosaic Virus ◽

Watermelon Mosaic Virus ◽

Cucumis Sativus L ◽

First Report

In August of 2002, cucumber (Cucumis sativus L. cv. Himangi) plants grown in commercial fields in Pune, western Maharashtra, India, exhibited chlorotic spots, veinal chlorosis, mosaic, blister formation and shoestring symptoms on leaves, stunted growth, and distortion of fruits. Incidence of virus infection in the fields varied from 25 to 38%. Crude sap extracted from infected cucumber leaf samples was inoculated mechanically onto cucumber and indicator host plants. The inoculated glasshouse-grown cucumber plants showed virus symptoms similar to those observed in the field. The virus produced chlorotic local lesions on Chenopodium amaranticolor and chlorotic spots followed by veinal chlorosis, mosaic, vein banding, and leaf distortion on Citrullus lanatus, C. melo, C. sativus, Cucurbita maxima, Cucurbita pepo, Luffa acutangula, and Trichosanthes anguina. The virus did not infect Nicotiana benthamiana, N. glutinosa, and N. tabacum cv. White Burley (1,2). Cucumber and indicator plants were tested using direct antigen coating enzyme-linked immunosorbent assays. A positive reaction was obtained with monoclonal antiserum to Zucchini yellow mosaic virus (ZYMV) but not with antisera to Papaya ringspot virus-P, Cucumber mosaic virus, and Watermelon mosaic virus (Agdia, Inc., Elkhart, IN). The disease was observed again in the field during July to September 2003. Natural infection of zucchini (Cucurbita pepo) by ZYMV has been reported previously from India (3). To our knowledge, this is the first report of ZYMV in cucumber in India. References: (1) H. Pospieszny et al. Plant Dis. 87:1399, 2003. (2) R. Provvidenti et al. Plant Dis. 68:443, 1984. (3) S. J. Singh et al. Indian Phytopathol. 56:174, 2003.

Download Full-text

Identificação sorológica de espécies de vírus que infetam cucurbitáceas em áreas produtoras do Maranhão

Fitopatologia Brasileira ◽

10.1590/s0100-41582001000100016 ◽

2001 ◽

Vol 26 (1) ◽

pp. 90-92 ◽

Cited By ~ 8

Author(s):

MARIA C. C. L. MOURA ◽

J. ALBERSIO A. LIMA ◽

VANÚZIA B. OLIVEIRA ◽

M. FÁTIMA B. GONÇALVES

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Immunosorbent Assay ◽

Citrullus Lanatus ◽

Zucchini Yellow Mosaic Virus ◽

Yellow Mosaic Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Watermelon Mosaic Virus ◽

Papaya Ringspot Virus ◽

Cucurbita Moschata

Os vírus representam sérios obstáculos para o sucesso da olericultura no mundo inteiro, constituindo a identificação daqueles de maior incidência numa região, papel fundamental para o estabelecimento de estratégias de controle. Visitas de campo foram realizadas a plantios de espécies de cucurbitáceas em áreas produtoras do Maranhão e amostras foliares foram coletadas de 118 plantas com sintomas ou suspeita de sintomas de vírus, sendo 46 de abóbora (Cucurbita moschata), 30 de melancia (Citrullus lanatus), 23 de maxixe (Cucumis anguria), 13 de pepino (C. sativus) e seis de melão (C. melo). Todas as amostras foram testadas contra anti-soros específicos para os principais vírus das famílias Bromoviridae, Comoviridae e Potyviridae que infetam cucurbitáceas no Nordeste, mediante "enzyme-linked immunosorbent assay" (ELISA) indireto e dupla difusão em agar. Os resultados revelaram a identificação sorológica de Papaya ringspot vírus (PRSV) em 64,4% das amostras analisadas, seguido de Watermelon mosaic virus-2 (WMV-2) em 15,2%, Cucumber mosaic virus (CMV) em 6,8%, Squash mosaic virus (SqMV) em 3,4% e Zucchini yellow mosaic virus (ZYMV) em 3,4%. Este levantamento confirma a predominância do PRSV em espécies de cucurbitáceas cultivadas no estado do Maranhão.

Download Full-text

Efeitos da interação de potyvirus em híbridos de meloeiro, variedades de melancia e abobrinha

Fitopatologia Brasileira ◽

10.1590/s0100-41582003000200016 ◽

2003 ◽

Vol 28 (2) ◽

pp. 199-204 ◽

Cited By ~ 8

Author(s):

Najara F. Ramos ◽

J. Albérsio A. Lima ◽

M. Fátima B. Gonçalves

Keyword(s):

Mosaic Virus ◽

Cucumis Melo ◽

Cucurbita Pepo ◽

Citrullus Lanatus ◽

Zucchini Yellow Mosaic Virus ◽

Ringspot Virus ◽

Yellow Mosaic Virus ◽

Watermelon Mosaic Virus ◽

Gold Mine ◽

Papaya Ringspot Virus

Em razão da freqüente ocorrência de infecção mista, na natureza, o presente trabalho objetivou estudar o efeito da interação de diferentes espécies de potyvírus em meloeiro (Cucumis melo), melancia (Citrullus lanatus) e abobrinha (Cucurbita pepo). Foram usados os seguintes vírus da família Potyviridae, gênero Potyvirus: Papaya ringspot virus (PRSV); Watermelon mosaic virus, (WMV) e Zucchini yellow mosaic virus, (ZYMV). Os efeitos na sintomatologia das infecções duplas e simples de PRSV, WMV e ZYMV foram avaliados em três híbridos de meloeiro, duas variedades de melancia e abobrinha 'Caserta', em experimentos de casa de vegetação. Os três vírus, isoladamente ou em todas as duplas combinações possíveis, foram inoculados, em plantas dos híbridos de meloeiro Hy Mark, Gold Mine e Orange Flesh, variedades de melancia Crimson Sweet e Charleston Gray e abobrinha 'Caserta', usando-se dez plantas de cada híbrido ou variedade, por combinação de vírus. As inoculações foram efetuadas por meio de extratos de folhas com infecção simples dos respectivos vírus. As plantas inoculadas com cada vírus isoladamente e suas respectivas combinações foram observadas quanto ao aparecimento de sintomas durante 30 dias após as inoculações. Amostras foliares das plantas inoculadas foram, também, testadas por ELISA indireto contra os anti-soros correspondentes para cada vírus. As infecções duplas em meloeiro, melancia e abobrinha revelaram, através da avaliação sintomatológica, que existem interações sinérgicas entre PRSV, WMV e ZYMV. As infecções duplas envolvendo o ZYMV apresentaram alta severidade, exibindo sintomas não encontrados em infecções simples, apesar da severidade nas infecções isoladas do ZYMV.

Download Full-text

High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

Pathogens ◽

10.3390/pathogens10010053 ◽

2021 ◽

Vol 10 (1) ◽

pp. 53

Author(s):

Vivek Khanal ◽

Harrington Wells ◽

Akhtar Ali

Keyword(s):

Phylogenetic Analysis ◽

Mosaic Virus ◽

Zucchini Yellow Mosaic Virus ◽

Yellow Mosaic Virus ◽

Watermelon Mosaic Virus ◽

High Prevalence ◽

The Us ◽

Close Relationship ◽

The Status ◽

Low Incidence

Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016–2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.

Download Full-text

Herança da resistência ao Watermelon mosaic virus em melancia (Citrullus lanatus L.)

Fitopatologia Brasileira ◽

10.1590/s0100-41582006000300010 ◽

2006 ◽

Vol 31 (3) ◽

pp. 302-305 ◽

Cited By ~ 7

Author(s):

J. Evando A. Beserra Júnior ◽

Wilson R. Maluf ◽

Antonia R. Figueira ◽

Beatriz M. Barguil

Keyword(s):

Mosaic Virus ◽

Citrullus Lanatus ◽

Watermelon Mosaic Virus

Foi estudado o controle genético da resistência do genótipo não-comercial de melancia, PI 595201, ao vírus do mosaico da melancia (Watermelon mosaic virus, WMV). Avaliaram-se os genitores, a cultivar Crimson Sweet (P1 - suscetível) e a introdução PI 595201 (P2 - resistente), bem como as populações F1, F2, e os retrocruzamentos para ambos os parentais, RC11 (F1 x P1) e RC12 (F1 x P2). A severidade dos sintomas, depois da inoculação mecânica com WMV, foi avaliada de acordo com uma escala de notas de 1 (folhas sem sintomas) a 5 (mosaico intenso e deformações foliares). A cultivar Crimson Sweet apresentou média geral acima de 4,0, enquanto a introdução PI 595201 apresentou média 1,0, confirmando a reação contrastante das linhagens parentais. A hipótese de herança monogênica foi rejeitada, mostrando ser a resistência da introdução PI 595201 de controle oligo ou poligênico, com indicativo de dominância completa no sentido de maior resistência ao vírus. A estimativa de herdabilidade no sentido amplo foi acima de 0,8. A estimativa do número de genes, controlando o caráter, foi 4,16. O modelo aditivo-dominante é sugerido para explicar o controle genético da resistência.

Download Full-text

Draft Genome Sequences of Bacillus and Paenibacillus Species Isolated from Seeds of Citrullus lanata (Watermelon), Cucurbita moschata (Butternut Squash), and Cucurbita pepo L. var. pepo L. (Pumpkin)

Microbiology Resource Announcements ◽

10.1128/mra.00727-20 ◽

2020 ◽

Vol 9 (34) ◽

Author(s):

Eman M. Khalaf ◽

Manish N. Raizada

Keyword(s):

Cucurbita Pepo ◽

Draft Genome ◽

Cucurbita Moschata ◽

Genome Sequences ◽

Content Type ◽

Paenibacillus Species

ABSTRACT Here, we announce the draft genome sequences of four endophytic bacilli isolated from surface-sterilized seeds of three cucurbit species, Bacillus sp. strains EKM417B and EKM420B (from Citrullus lanata [watermelon]) and EKM501B (from Cucurbita moschata [butternut squash]) and Paenibacillus sp. strain EKM301P (from Cucurbita pepo L. var. pepo L. [pumpkin]). These strains previously demonstrated biostimulant and biocontrol activities.

Download Full-text

Serological and Molecular Characterisation of Virus Infecting Watermelon (Citrullus lanatus) in Adim-Biase, Cross River State, Nigeria

Annual Research & Review in Biology ◽

10.9734/arrb/2020/v35i1130300 ◽

2020 ◽

pp. 66-72

Author(s):

O. I. Eyong ◽

E. E. Ekpiken ◽

G. M. Ubi ◽

A. O. Alobi

Keyword(s):

Mosaic Virus ◽

Virus Disease ◽

Citrullus Lanatus ◽

Good Health ◽

Watermelon Mosaic Virus ◽

Cross River ◽

Cross River State ◽

Gene Sequence Analysis ◽

Cucumeropsis Mannii ◽

Cucurbitaceae Family

Watermelon, Citrullus lanatus, (Thunb.) is an annual crop in the Cucurbitaceae family grown for it fruits. It nutritional and medical uses helps in combating some illnesses and maintaining good health. The production of this crop in Nigeria is seriously under threat by virus disease. A survey of some farms in Adim, Biase Local Government Area of Cross River State, Nigeria during the 2019 planting season revealed mosaic and chlorotic symptoms. Mechanical inoculation was performed on young seedlings of Cucumeropsis mannii from watermelon samples showing viral symptoms and latter tested against the universal potyvirus antiserum and further detected by RT-PCR assay using cylindrical inclusion (CI) primer. The amplified cDNA was cloned and the nucleotide sequence determined. The serology result showed that there was positive reaction against the potyvirus antiserum confirming it to be a potyvirus. The gene sequence analysis revealed 86% sequence identity with Algerian watermelon mosaic virus. This is the first report of Algerian watermelon mosaic virus strain infecting watermelon in Nigeria.

Download Full-text

Evaluation of ‘AU-Performance’ Watermelon for Its Response to Virus Inoculation

HortTechnology ◽

10.21273/hortsci.19.3.609 ◽

2009 ◽

Vol 19 (3) ◽

pp. 609-612 ◽

Cited By ~ 1

Author(s):

John F. Murphy ◽

Fenny Dane

Keyword(s):

Mosaic Virus ◽

Fungal Pathogens ◽

Citrullus Lanatus ◽

Zucchini Yellow Mosaic Virus ◽

Systemic Infection ◽

Yellow Mosaic Virus ◽

Watermelon Mosaic Virus ◽

Susceptible Parent ◽

Resistant Parent ◽

Systemic Symptoms

The watermelon (Citrullus lanatus var. lanatus) ‘AU-Performance’ was developed for resistance to multiple fungal pathogens and the plant virus, zucchini yellow mosaic virus (ZYMV). A greenhouse-based evaluation was carried out to determine the response of ‘AU-Performance’ to inoculation with three important cucurbit (Cucurbitaceae) viruses in the genus Potyvirus: papaya ringspot virus (PRSV), watermelon mosaic virus (WMV), and ZYMV. The evaluation included the resistant parent (PI595203), the susceptible parent (‘AU-Producer’), and varieties AU-Allsweet and Charleston Gray. Each of the three viruses systemically infected ‘AU-Performance’ with 100% infection and development of characteristic systemic symptoms. The susceptible parent (‘AU-Producer’), ‘AU-Allsweet’, and ‘Charleston Gray’ responded similarly with 100% infection and systemic symptoms. In contrast, the resistant parent (PI595203) was resistant to WMV and ZYMV; however, PRSV-inoculated plants developed a systemic infection with accompanied symptoms and high levels of PRSV accumulation in noninoculated leaves. PI595203 was shown in previous studies to be resistant to PRSV. We show in this report that under greenhouse conditions and application of virus by mechanical inoculation, ‘AU-Performance’ was not resistant to infection by the three potyviruses.

Download Full-text

Creation of trivalent transgenic watermelon resistant to virus infection

Chinese Journal of Agricultural Biotechnology ◽

10.1079/cjb200568 ◽

2005 ◽

Vol 2 (3) ◽

pp. 179-185 ◽

Cited By ~ 1

Author(s):

Niu Sheng-Niao ◽

Huang Xue-Sen ◽

Wong Sek-Man ◽

Yu Jia-Lin ◽

Zhao Fu-Xing ◽

...

Keyword(s):

Virus Infection ◽

Mosaic Virus ◽

Citrullus Lanatus ◽

Zucchini Yellow Mosaic Virus ◽

Field Trials ◽

Yellow Mosaic Virus ◽

Watermelon Mosaic Virus ◽

Virus Infections ◽

High Level ◽

Transgenic Watermelon

AbstractA trivalent vector containing genes of Watermelon mosaic virus (WMV) coat protein (CP), and replicase genes of Zucchini yellow mosaic virus (ZYMV) and Cucumber mosaic virus (CMV), was constructed for transformation of watermelon (Citrullus lanatus) plants, mediated by Agrobacterium tumefaciens. The integrated foreign genes were identified in the regenerated progenies by polymerase chain reaction (PCR) and Southern blots. The transformation efficiency was about 1.7‰. Resistance to virus infection was determined by mechanical inoculation in the greenhouse and by field trials. The transgenic watermelon lines showed different phenotypes of susceptible, resistant, immune or recovery from virus infections in the late growth stage. A relatively high level of resistance was shown by T3 plants of the line BH1-7. This result indicates the possibility of creating, by transgenic protocols, new varieties of watermelon resistant to viral infection.

Download Full-text