scholarly journals Phenotypic and Genetic Characterization of Botrytis cinerea Population from Kiwifruit in Sichuan Province, China

Plant Disease ◽  
2019 ◽  
Vol 103 (4) ◽  
pp. 748-758 ◽  
Author(s):  
Yan-gang Pei ◽  
Qin-jun Tao ◽  
Xiao-juan Zheng ◽  
Ying Li ◽  
Xiao-fang Sun ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Sensu Stricto ◽  
Sichuan Province ◽  
Genetic Characteristics ◽  
Morphological Criteria ◽  
Young Leaves ◽  
Pathogen Variability ◽  
Botryotinia Fuckeliana ◽  
Phenotypic And Genetic Characterization

Botrytis cinerea (anamorph of Botryotinia fuckeliana) causes gray mold on numerous plants, including kiwifruit. The primary aim of this study was to investigate the phenotypic and genetic characteristics of the Botrytis cinerea population from kiwifruit in Sichuan Province, China. In all, 176 isolates were collected from kiwifruit orchards from eight geographic regions in Sichuan. All isolates were identified as B. cinerea sensu stricto based on the combined datasets, including morphological criteria, determination of the Bc-hch allele, and phylogenetic analysis of the genes RPB2, G3PDH, and HSP60. Three colony types (i.e., sclerotial, mycelial, and conidial) were observed on potato dextrose agar after 2 weeks, with sclerotial isolates, the predominant category, accounting for 40.91%. No obvious differences in microscopic characteristics were observed among the three types. Three genotypes of transposable elements were identified in the B. cinerea population: boty, flipper, and transposa types. The most prevalent genotype from different geographic populations of B. cinerea was transposa; in contrast, the flipper genotype accounted for only 3.98% of the total population, whereas the vacuma genotype was absent. According to MAT locus amplification, 87 and 89 isolates are MAT1-1 and MAT1-2 type, respectively, and the two mating types were found to be balanced overall in the population. Forty-eight representative isolates were all able to cause gray mold to some extent, and disease severities were significantly different between the cultivars Hongyang and Hort16A (P < 0.01). Disease severity was significantly greater on young leaves than on mature leaves (P < 0.01). No significant relationship was found between pathogenicity and geographical region, colony type, or transposon distribution. The results obtained in the present study suggest a relatively uniform species diversity of Botrytis but rich phenotypic and genetic differentiation within the B. cinerea population on kiwifruit in China. Utilizing resistant cultivars and rain-shelter cultivation instead of fungicides may be an effective approach to delaying pathogen variability.

scholarly journals First Report of Fenhexamid-Resistant Botrytis cinerea Causing Gray Mold on Heurchera in a North American Greenhouse

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 147-147 ◽  
Author(s):  
G. W. Moorman ◽  
A.-S. Walker ◽  
S. May
Keyword(s):  
Botrytis Cinerea ◽  
Mode Of Action ◽  
Gray Mold ◽  
North Central ◽  
First Report ◽  
Group I ◽  
Central Pennsylvania ◽  
Commercial Operation ◽  
Penn State ◽  
Botryotinia Fuckeliana

Greenhouse-grown Heuchera plants, treated with fenhexamid (Decree, SePRO, Carmel, IN; FRAC group 17 hydroxyanilide), with active gray mold were submitted to the Penn State Plant Disease Clinic in December 2010 from a commercial operation in north-central Pennsylvania. Genetic and phenotypic analyses identified the isolate as Botrytis cinerea Pers. (teleomorph Botryotinia fuckeliana (de Bary) Whetzel), HydR3 phenotype (2) and not B. pseudocinerea (previously Botrytis group I) (4), naturally resistant to fenhexamid (phenotype HydR1) (1). While 0.2 μg of fenhexamid per ml or less is required to slow mycelial growth and germ tube elongation of sensitive isolates by 50% (EC50), the radial growth EC50 of the Heuchera isolate was approximately 2,000 μg of fenhexamid per ml in culture. Five cucumber seedlings receiving 25 μl of 0.1 M dextrose containing the label rate of Decree (1,800 μg/ml) on the growing tip were inoculated with colonized agar in the drop. Five check plants received 25 μl of 0.1 M dextrose. B. cinerea from silica gel storage since 1988 was also tested. This experiment was repeated three times. The 1988 isolate killed all fungicide-free but no fenhexamid-treated plants. The Heuchera isolate killed all fungicide-free and fenhexamid-treated plants within 4 days. To our knowledge, this is the first report of B. cinerea from a greenhouse in North America with fenhexamid resistance. Resistance occurs in U.S. fields (3). The Heuchera isolate's HydR3 resistance phenotype (2) has been detected in Germany, Japan, and France and has mutations affecting the 3-keto reductase protein, encoded by the erg27 gene, the specific target of fenhexamid and involved in Botrytis sterol biosynthesis. The Decree label states that it is to be used only twice on a crop before switching to a different mode of action. Greenhouses have resident Botrytis populations that are likely to be exposed to any fungicide applied in the structure. Growers should consider using fenhexamid only twice in a particular greenhouse, rather than on a particular crop, before switching to a different mode of action. References: (1) P. Leroux et al. Crop Prot. 18:687, 1999.(2) P. Leroux et al. Pest Manag. Sci. 58:876, 2002. (3) Z. Ma and T. J. Michailides. Plant Dis. 89:1083, 2005. (4) A.-S. Walker et al. Phytopathology 101:1433, 2011.

scholarly journals Botrytis pseudocinerea, a New Cryptic Species Causing Gray Mold in French Vineyards in Sympatry with Botrytis cinerea

2011 ◽  
Vol 101 (12) ◽  
pp. 1433-1445 ◽  
Author(s):  
Anne-Sophie Walker ◽  
Angélique Gautier ◽  
Johann Confais ◽  
Daniel Martinho ◽  
Muriel Viaud ◽  
...  
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Botrytis Cinerea ◽  
Germination Rate ◽  
Gray Mold ◽  
Temperature Adaptation ◽  
Morphological Criteria ◽  
Ecological Criteria ◽  
High Level

Botrytis cinerea is a major crop pathogen infesting >220 hosts worldwide. A cryptic species has been identified in some French populations but the new species, B. pseudocinerea, has not been fully delimited and established. The aim of this study was to distinguish between the two species, using phylogenetic, biological, morphological, and ecological criteria. Multiple gene genealogies confirmed that the two species belonged to different, well-supported phylogenetic clades. None of the morphological criteria tested (spore size, germination rate, or mycelial growth) was able to discriminate between these two species. Sexual crosses between individuals from the same species and different species were carried out. Only crosses between individuals from the same species were successful. Moreover, population genetics analysis revealed a high level of diversity within each species and a lack of gene flow between them. Finally, a population survey over time showed that B. cinerea was the predominant species but that B. pseudocinerea was more abundant in spring, on floral debris. This observation could not be explained by temperature adaptation in tests carried out in vitro or by aggressiveness on tomato or bean leaves. This study clearly establishes that B. cinerea and B. pseudocinerea constitute a complex of two cryptic species living in sympatry on several hosts, including grapevine and blackberry. We propose several biological or molecular tools for unambiguous differentiation between the two species. B. pseudocinerea probably makes a negligible contribution to gray mold epidemics on grapevine. This new species has been deposited in the MycoBank international database.

scholarly journals Morphological, pathogenic and genetic diversity of Botrytis cinerea Pers. in blackberry cultivations in Colombia

2019 ◽  
Vol 41 (6) ◽  
Author(s):  
Liliana Isaza ◽  
Yenni Paola Zuluaga ◽  
Marta Leonor Marulanda
Keyword(s):  
Genetic Diversity ◽  
Botrytis Cinerea ◽  
High Resistance ◽  
Geographical Origin ◽  
Genetic Characterization ◽  
Gray Mold ◽  
Daily Growth ◽  
Genetic Characteristics ◽  
Management Measures ◽  
Pathogenicity Tests

Abstract The Andean blackberry (Rubus glaucus Benth) is one of the most important fruits with the greatest commercial projection in Colombia. The factors that most affect its production are the attack of diseases and the scarce information about its control. One of the most limiting diseases of the crop is the gray mold, produced by the fungus Botrytis cinerea. This research presents the diversity of B. cinerea isolates from fields of andean blackberry in Colombia, based on the morphological, pathogenic and genetic characteristics allowing broadening the knowledge of the phytopathogen for subsequent management measures. Andean blackberry fruits were collected with characteristic symptoms of gray mold in farms located in eight producing areas of Colombian Andean region. A total of 50 samples were analyzed finding two types of growth, miceliar and sclerocial, differences in the layout and size of the sclerotia, as well as differences in the daily growth of the mycelium. Regarding genetic characterization with microsatellite markers, it was shown that genetic diversity is concentrated within populations and that there is a tendency to group by geographical origin. With the pathogenicity tests, the two most pathogenic isolates were selected and it was found that, although the four possible genotypes were found with the transposable elements (Vacuma, Transposa, Boty, Flipper), none of them presented high resistance to the fungicide fenhexamide.

scholarly journals Dasylirion serratifolium as a New Host of Botrytis cinerea, the Causal Agent of Leaf Spots and Blight in Italy

Plant Disease ◽  
2006 ◽  
Vol 90 (1) ◽  
pp. 114-114 ◽  
Author(s):  
G. Polizzi ◽  
A. Vitale
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Potato Dextrose Agar ◽  
Limiting Factor ◽  
Sterile Water ◽  
New Host ◽  
Leaf Spots ◽  
Brown Stripe ◽  
Young Leaves ◽  
International Mycological Institute

Sandpaper sotol (Dasylirion serratifolium Zucc.), native to Mexico, has green leaves with margins highlighted by whitish yellow prickles like a fine sandpaper. During the spring of 2004 and 2005, necrotic lesions were observed in the middle of leaf blades and near prickles on 2- to 5-year-old, container-grown sandpaper sotol plants from two nurseries in eastern Sicily (Italy). Symptoms were detected on 20 to 30% of plants and consisted of reddish lesions that developed a reddish brown stripe surrounded by a yellow halo. As lesions enlarged, the center turned yellow and expanded rapidly causing blight of young leaves. Occasionally, symptomatic tissues had masses of gray fungal conidia and/or sclerotia. Botrytis cinerea was isolated consistently from infected tissues disinfected for 1 min in 1% NaOCl and rinsed in sterile distilled water and grown on potato dextrose agar (PDA). Hyaline, ovoid conidia (average 6.4 × 9.7 μm) and conidiophores were similar to those described of B. cinerea, and 5- to 8-day-old cultures developed black sclerotia that were round or irregular in shape (average 1.55 × 1.02 mm) that is typical of gray mold (1). Koch's postulates were performed by spraying 6-week-old sandpaper sotol plants grown in 12-cm pots with a spore suspension (1 × 106 CFU per ml) obtained from 12-day-old cultures grown on PDA. Eight plants were naturally wounded by scratching leaf blades among themselves and were subsequently inoculated, while eight plants were inoculated without wounding. An equal number of noninoculated plants sprayed with sterile water served as controls. All plants were maintained in high humidity conditions (90 to 95% relative humidity) at 20 ± 2°C. Leaf spots similar to the ones observed in nurseries were evident on all naturally wounded and nonwounded plants within 2 to 3 weeks after inoculation. Noninoculated control plants were symptomless. B. cinerea was reisolated from affected tissues. The pathogen has reduced commercial value of sandpaper sotol plants and may represent a limiting factor for the cultivation of this plant in eastern Sicily. To our knowledge, this is the first report in the world of leaf spot and blight caused by B. cinerea on D. serratifolium. Reference: (1) M. B. Ellis. Dematiaceous Hyphomycetes. CAB International Mycological Institute, Kew, Surrey, England, 1971.

scholarly journals First Report of Botrytis cinerea Causing Gray Mold of Tobacco in Guizhou Province of China

Plant Disease ◽  
2011 ◽  
Vol 95 (5) ◽  
pp. 612-612 ◽  
Author(s):  
H.-C. Wang ◽  
W.-H. Li ◽  
M.-S. Wang ◽  
Q.-Y. Chen ◽  
Y.-G. Feng ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Management Practices ◽  
Northern China ◽  
Gray Mold ◽  
Guizhou Province ◽  
Universal Primers ◽  
Its2 Region ◽  
First Report ◽  
Nicotiana Tabacum L ◽  
Botryotinia Fuckeliana

Tobacco (Nicotiana tabacum L.) is a leafy, annual, solanaceous plant grown commercially for its leaves. Guizhou Province produces more than 30% of the total Chinese tobacco crop. In July 2010, a disease was observed in a commercial field of 5-month-old N. tabacum plants in Bijie, Guizhou in southwestern China. Symptoms first appeared on the leaves as small spots that later increased in size and developed into expanded, dark brown lesions covered with green-gray spore masses. Lesions expanded rapidly under cool, humid conditions. Isolates of Botrytis cinerea were collected from diseased leaves with typical symptoms. Diseased leaf samples were washed with distilled water three times, placed in a moist chamber, and incubated at 25°C in darkness for 48 h to encourage sporulation. Spores produced on leaves were transferred to individual agar discs (5 mm in diameter) with an inoculating needle and then the agar discs were transferred to potato dextrose agar (PDA) and incubated at 25°C for 7 days. Fungal colonies were at first colorless and later became gray to brown when the conidia differentiated. The size of conidia ranged from 5.0 to 9.5 × 6.5 to 12.5 μm (average 7.3 × 8.7 μm) based on 50 spore measurements. Microsclerotia produced in the culture were round or irregular and ranged from 1.2 to 3.0 × 1.0 to 2.5 mm (average 2.1 × 2.0 mm). The pathogen was identified as B. cinerea Pers.:Fr on the basis of morphology and sequence of ITS1-5.8s-ITS2 region of rDNA amplified by PCR using universal primers ITS-1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS-4 (5′-TCCTCCGCTTATTGATATGC-3′). The sequence (GenBank Accession No. HQ902163) exactly matched the sequences of two Botryotinia fuckeliana (anamorph B. cinerea) accessions, (e.g., GenBank Accession Nos. HM849615.1 and HM849047.1). Koch's postulates were conducted by wound inoculating five tobacco leaves (cv. K326) after surface disinfesting them with 5% NaOCl. Plugs of the fungus (5 mm in diameter) obtained from the colony margins were transferred onto 3 × 3 mm wounds made with a needle on the surface of five sterilized leaves. Inoculated leaves were incubated at 25°C, 100 to 120 μE·m–2·s–1, relative humidity >80%, and 16 h light per day for disease development. Typical symptoms developed on leaves within 7 days after inoculation. The pathogen was reisolated from affected leaves but not from the noninoculated control leaves. Botrytis gray mold blight has been recorded on N. tabacum in New Zealand, the United Kingdom, and northern China (1–3). However, to our knowledge, this is the first report of Botrytis blight on N. tabacum in Guizhou Province of China and the disease must be considered in existing disease management practices. References: (1) W. Brian et al. Mol. Plant Pathol. 8:561, 2007. (2) A. G. Mcleod et al. N. Z. J. Crop Hortic. Sci/Exp. Agric. 12:866, 1958. (3) Z. Y. Zhang. Page 37 in: Flora Fungorum Sinicorum. Vol. 26. Science Press, Beijing, 2006.

scholarly journals Cyclophilin BcCyp2 Regulates Infection-Related Development to Facilitate Virulence of the Gray Mold Fungus Botrytis cinerea

2021 ◽  
Vol 22 (4) ◽  
pp. 1694
Author(s):  
Jiao Sun ◽  
Chen-Hao Sun ◽  
Hao-Wu Chang ◽  
Song Yang ◽  
Yue Liu ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Gray Mold ◽  
Gene Encoding ◽  
Histone 3 ◽  
Mold Fungus ◽  
Related Development ◽  
Hyphal Morphology ◽  
Cellular Components

Cyclophilin (Cyp) and Ca2+/calcineurin proteins are cellular components related to fungal morphogenesis and virulence; however, their roles in mediating the pathogenesis of Botrytis cinerea, the causative agent of gray mold on over 1000 plant species, remain largely unexplored. Here, we show that disruption of cyclophilin gene BcCYP2 did not impair the pathogen mycelial growth, osmotic and oxidative stress adaptation as well as cell wall integrity, but delayed conidial germination and germling development, altered conidial and sclerotial morphology, reduced infection cushion (IC) formation, sclerotial production and virulence. Exogenous cyclic adenosine monophosphate (cAMP) rescued the deficiency of IC formation of the ∆Bccyp2 mutants, and exogenous cyclosporine A (CsA), an inhibitor targeting cyclophilins, altered hyphal morphology and prevented host-cell penetration in the BcCYP2 harboring strains. Moreover, calcineurin-dependent (CND) genes are differentially expressed in strains losing BcCYP2 in the presence of CsA, suggesting that BcCyp2 functions in the upstream of cAMP- and Ca2+/calcineurin-dependent signaling pathways. Interestingly, during IC formation, expression of BcCYP2 is downregulated in a mutant losing BcJAR1, a gene encoding histone 3 lysine 4 (H3K4) demethylase that regulates fungal development and pathogenesis, in B. cinerea, implying that BcCyp2 functions under the control of BcJar1. Collectively, our findings provide new insights into cyclophilins mediating the pathogenesis of B. cinerea and potential targets for drug intervention for fungal diseases.

scholarly journals Characterization of Postharvest Fungicide-Resistant Botrytis cinerea Isolates From Commercially Stored Apple Fruit

2017 ◽  
Vol 107 (3) ◽  
pp. 362-368 ◽  
Author(s):  
Wayne M. Jurick ◽  
Otilia Macarisin ◽  
Verneta L. Gaskins ◽  
Eunhee Park ◽  
Jiujiang Yu ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Apple Fruit ◽  
Sublethal Dose ◽  
Long Term Storage ◽  
Pose Control ◽  
First Time

Botrytis cinerea causes gray mold and is an economically important postharvest pathogen of fruit, vegetables, and ornamentals. Fludioxonil-sensitive B. cinerea isolates were collected in 2011 and 2013 from commercial storage in Pennsylvania. Eight isolates had values for effective concentrations for inhibiting 50% of mycelial growth of 0.0004 to 0.0038 μg/ml for fludioxonil and were dual resistant to pyrimethanil and thiabendazole. Resistance was generated in vitro, following exposure to a sublethal dose of fludioxonil, in seven of eight dual-resistant B. cinerea isolates. Three vigorously growing B. cinerea isolates with multiresistance to postharvest fungicides were further characterized and found to be osmosensitive and retained resistance in the absence of selection pressure. A representative multiresistant B. cinerea strain caused decay on apple fruit treated with postharvest fungicides, which confirmed the in vitro results. The R632I mutation in the Mrr1 gene, associated with fludioxonil resistance in B. cinerea, was not detected in multipostharvest fungicide-resistant B. cinerea isolates, suggesting that the fungus may be using additional mechanisms to mediate resistance. Results from this study show for the first time that B. cinerea with dual resistance to pyrimethanil and thiabendazole can also rapidly develop resistance to fludioxonil, which may pose control challenges in the packinghouse environment and during long-term storage.

The potential biocontrol agent Paenibacillus polymyxa TP3 produces fusaricidin-type compounds involved in the antagonism against gray mold pathogen Botrytis cinerea

2021 ◽  
Author(s):  
Shuen-Huang Tsai ◽  
Yu-Ting Chen ◽  
Yu-Liang Yang ◽  
Bo-Yi Lee ◽  
Chien-Jui Huang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Botrytis Cinerea ◽  
Biological Control Agent ◽  
Imaging Mass Spectrometry ◽  
Paenibacillus Polymyxa ◽  
Field Trials ◽  
Gray Mold ◽  
Control Agent ◽  
Antagonistic Activity ◽  
New Variant

Paenibacillus polymyxa is a beneficial bacterium for plant health. Paenibacillus polymyxa TP3 exhibits antagonistic activity toward Botrytis cinerea and alleviates gray mold symptoms on the leaves of strawberry plants. Moreover, suppression of gray mold on the flowers and fruits of strawberry plants in field trials, including vegetative cells and endospores, was demonstrated, indicating the potential of strain TP3 as a biological control agent. To examine the anti-B. cinerea compounds produced by P. polymyxa TP3, matrix‐assisted laser‐desorption/ionization time‐of‐flight mass spectrometry was performed and fusaricidin-corresponding mass spectra were detected. Moreover, fusaricidin-related signals appeared in imaging mass spectrometry of TP3 when confronted with B. cinerea. By using liquid chromatography-mass spectrometry-based molecular networking approach, several fusaricidins were identified including a new variant of m/z 917.5455 with serine in the first position of the hexapeptide. Via advanced mass spectrometry and network analysis, fusaricidin-type compounds produced by P. polymyxa TP3 were efficiently disclosed and were presumed to play roles in the antagonism against gray mold pathogen B. cinerea.

Gray mold of yacon and sunflower caused by Botrytis cinerea

2011 ◽  
Vol 77 (3) ◽  
pp. 217-219 ◽  
Author(s):  
Keisuke Tomioka ◽  
Toyozo Sato
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold
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