scholarly journals Identification of Blast Resistance in a Core Collection of Foxtail Millet Germplasm

Plant Disease ◽  
2014 ◽  
Vol 98 (4) ◽  
pp. 519-524 ◽  
Author(s):  
Rajan Sharma ◽  
A. G. Girish ◽  
H. D. Upadhyaya ◽  
P. Humayun ◽  
T. K. Babu ◽  
...  
Keyword(s):  
Core Collection ◽  
Magnaporthe Grisea ◽  
Blast Resistance ◽  
Foxtail Millet ◽  
Leaf Sheath ◽  
Leaf Blast ◽  
Blast Score ◽  
Serious Disease ◽  
Seedling Leaf ◽  
Yield Trials

Blast, also known as leaf spot, caused by Pyricularia grisea (teleomorph: Magnaporthe grisea), is a serious disease affecting both forage and grain production in foxtail millet in India. For the identification of new and diverse sources of blast resistance, a foxtail millet core collection comprising 155 accessions was evaluated against the Patancheru isolate (Fx 57) of M. grisea. In a field screen during 2009 and 2010, 21 accessions were identified with neck and head blast resistance against Fx 57. In a greenhouse screen, 11 of the 155 accessions exhibited seedling leaf blast resistance to the same isolate. Further evaluation of the selected 28 accessions (found resistant to neck and head blast under field conditions during 2009 and 2010 or leaf blast in the greenhouse screen) against four M. grisea isolates (Fx 57, Fx 58, Fx 60, and Fx 62 from Patancheru, Nandyal, Vizianagaram, and Mandya, respectively) led to the identification of 16 accessions with leaf, sheath, neck, and head blast resistance to at least one isolate. Two accessions (ISe 1181 and ISe 1547) were free from head blast infection and showed resistance to leaf (score ≤3.0 on a 1-to-9 scale), neck, and sheath blast (score ≤2.0 on a 1-to-5 scale) against all four isolates. In addition, ISe 1067 and ISe 1575 also exhibited high levels of blast resistance. Blast-resistant accessions with superior agronomic and nutritional quality traits can be evaluated in multilocation yield trials before releasing them for cultivation to farmers.

scholarly journals Pathogenic Variation in the Pearl Millet Blast Pathogen Magnaporthe grisea and Identification of Resistance to Diverse Pathotypes

Plant Disease ◽  
2013 ◽  
Vol 97 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Rajan Sharma ◽  
H. D. Upadhyaya ◽  
S. V. Manjunatha ◽  
K. N. Rai ◽  
S. K. Gupta ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Magnaporthe Grisea ◽  
Blast Resistance ◽  
Uttar Pradesh ◽  
Yield Potential ◽  
Sources Of Resistance ◽  
Pathogenic Variation ◽  
Fodder Yield ◽  
Improvement Programs ◽  
Serious Disease

Blast, also known as leaf spot, caused by Pyricularia grisea (teleomorph: Magnaporthe grisea), has emerged as a serious disease affecting both forage and grain production in pearl millet in India. Pathogenic variation was studied in a greenhouse using 25 M. grisea isolates collected from four major pearl-millet-growing states in India (Rajasthan, Haryana, Maharashtra, and Uttar Pradesh) on 10 pearl millet genotypes (ICMB 02444, ICMB 02777, ICMB 06444, ICMB 93333, ICMB 96666, ICMB 97222, ICMB 99444, 863B, ICMR 06222, and ICMB 95444). Differential reactions to the test isolates were recorded on ICMB 02444, ICMB 93333, ICMB 97222, 863B, and ICMR 06222. The 25 isolates were grouped into five different pathotypes based on their reaction types (virulent = score ≥ 4 and avirulent = score ≤ 3 on a 1-to-9 scale). For the identification of resistance sources, a pearl millet mini-core comprising 238 accessions was evaluated under greenhouse conditions against five M. grisea isolates (Pg118, Pg119, Pg56, Pg53, and Pg45) representing the five pathotypes. Of 238 accessions, 32 were found to be resistant to at least one pathotype. Resistance to multiple pathotypes (two or more) was recorded in several accessions, while three accessions (IP 7846, IP 11036, and IP 21187) exhibited resistance to four of the five pathotypes. Four early-flowering (≤50 days) blast-resistant mini-core accessions (IP 7846, IP 4291, IP 15256, and IP 22449) and four accessions (IP 5964, IP 11010, IP 13636, and IP 20577) having high scores (≥7) for grain and green fodder yield potential and overall plant aspect were found to be promising for utilization in pearl millet improvement programs. Identification of five pathotypes of M. grisea and sources of resistance to these pathotypes will provide a foundation for breeding for blast resistance in pearl millet in India.

scholarly journals Natural Variation at the Pi-ta Rice Blast Resistance Locus

2003 ◽  
Vol 93 (11) ◽  
pp. 1452-1459 ◽  
Author(s):  
Yulin Jia ◽  
Gregory T. Bryan ◽  
Leonard Farrall ◽  
Barbara Valent
Keyword(s):  
Positional Cloning ◽  
Magnaporthe Grisea ◽  
Blast Resistance ◽  
El Paso ◽  
Avirulence Gene ◽  
Resistance Locus ◽  
Japonica Rice ◽  
Breeding Programs ◽  
Rice Blast Resistance ◽  
Intron Region

The resistance gene Pi-ta protects rice crops against the fungal pathogen Magnaporthe grisea expressing the avirulence gene AVR-Pita in a gene-for-gene manner. Pi-ta, originally introgressed into japonica rice from indica origin, was previously isolated by positional cloning. In this study, we report the nucleotide sequence of a 5,113-base pair region containing a japonica susceptibility pi-ta allele, which has overall 99.6% nucleotide identity to the indica Pi-ta allele conferring resistance. The intron region shows the levels of sequence diversity that typically differentiate genes from indica and japonica rices, but the other gene regions show less diversity. Sequences of the Pi-ta allele from resistant cultivars Katy and Drew from the southern United States are identical to the resistance Pi-ta sequence. Sequences from susceptible cultivars El Paso 144 and Cica 9 from Latin America define a third susceptibility haplotype. This brings the total number of Pi-ta haplotypes identified to four, including the resistance allele and three susceptibility alleles. The Pi-ta locus shows low levels of DNA polymorphism compared with other analyzed R genes. Understanding the natural diversity at the Pi-ta locus is important for designing specific markers for incorporation of this R gene into rice-breeding programs.

scholarly journals The OsOXO2, OsOXO3 and OsOXO4 Positively Regulate Panicle Blast Resistance in Rice

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jingfang Dong ◽  
Lian Zhou ◽  
Aiqing Feng ◽  
Shaohong Zhang ◽  
Hua Fu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Blast Resistance ◽  
Aba Signaling ◽  
Leaf Blast ◽  
Expression Levels ◽  
Panicle Blast ◽  
Localization Analysis ◽  
Sa Signaling

Abstract Background Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance are still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown. Results In the present study, we have confirmed that the three Oxalate oxidase (OXO) genes, OsOXO2, OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2, OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO proteins are all localized in the nucleus and cytoplasm. Simultaneous silencing of OsOXO2, OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2, OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance. More H2O2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the silencing plants exhibited less H2O2 and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the phytohormone signaling related gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the activation of JA and ABA signaling pathways but suppression of SA signaling pathway. Conclusion OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H2O2 and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance could be regulated by ABA, SA and JA, and may be associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway.

Marker Development for Rice Blast Resistance Gene Pi66(t) and Application in the USDA Rice Mini-Core Collection

Crop Science ◽  
2016 ◽  
Vol 56 (3) ◽  
pp. 1001-1008 ◽  
Author(s):  
Yan Liu ◽  
Yulin Jia ◽  
David Gealy ◽  
David M. Goad ◽  
Ana L. Caicedo ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Core Collection ◽  
Blast Resistance ◽  
Marker Development ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Mini Core Collection

scholarly journals Development of rice pre-breeding resources with blast resistance

2020 ◽  
Vol 224 ◽  
pp. 04020
Author(s):  
Zh M Mukhina ◽  
E G Savenko ◽  
T L Korotenko ◽  
I I Suprun ◽  
O. A. Bragina ◽  
...  
Keyword(s):  
Magnaporthe Grisea ◽  
Blast Resistance ◽  
Local Population ◽  
Rice Varieties ◽  
Germplasm Exchange ◽  
Yield Structure ◽  
Rice Samples ◽  
Breeding Resources

Within the framework of this study, the first Russian-Chinese joint program for development of rice varieties with long-term blast resistance was launched on the basis of rice germplasm exchange and the subsequent comprehensive study of the obtained breeding samples, hybridization of Russian and Chinese varieties with specified traits. The genetic diversity of the Chinese rice samples obtained by exchange was studied by biological and morphological traits of plants, taxonomic belonging to a botanical variety, elements of the yield structure, and resistance to lodging. 31 hybrid combinations (F1) from the crossing of Russian and Chinese varieties were used to obtain the BC1 generation. The technologies of cultivation of isolated rice anthers in vitro have been optimized in relation to Chinese genotypes in order to accelerate the genetic stabilization of breeding material obtained from crossing. Phenotyping of Russian and Chinese breeding samples was carried out on the basis of resistance to the local population of the blast pathogen (Magnaporthe grisea (T.T. Hebert) M.E. Barr)), on natural and artificial infectious backgrounds. DNA identification of genes for blast resistance was carried out for the same breeding samples. Based on the experimental data obtained, promising samples were selected - prototypes of new rice varieties.

scholarly journals Resistance to blast (Magnaporthe grisea) in a mini-core collection of finger millet germplasm

2012 ◽  
Vol 135 (2) ◽  
pp. 299-311 ◽  
Author(s):  
T. Kiran Babu ◽  
R. P. Thakur ◽  
H. D. Upadhyaya ◽  
P. N. Reddy ◽  
R. Sharma ◽  
...  
Keyword(s):  
Core Collection ◽  
Magnaporthe Grisea ◽  
Finger Millet ◽  
Mini Core Collection

scholarly journals Variation on agro-morphological traits in Nepalese foxtail millet (Setaria italica (L) P Beauv)

2013 ◽  
Vol 2 ◽  
pp. 133-138 ◽  
Author(s):  
RB Amgai ◽  
S Pantha ◽  
TB Chhetri ◽  
SK Budhathoki ◽  
SP Khatiwada ◽  
...  
Keyword(s):  
Flag Leaf ◽  
Foxtail Millet ◽  
Morphological Characteristics ◽  
Leaf Sheath ◽  
Kernel Weight ◽  
Leaf Length ◽  
Setaria Italica ◽  
Yield Enhancement ◽  
Peduncle Length ◽  
Flag Leaf Length

Foxtail millet (Setaria italica (L) P Beauv) falls on the category of underutilized crops in Nepal and mainly cultivated in Karnali region of the country. It is hardy crop and considered as one of the potential crops for future food security with respect to climate change. Five accessions of Nepalese foxtail millet were purposefully selected for evaluation of the agro-morphological characteristics. Foxtail landraces from Dolpa, Mugu, Bajura, Bajhang and Lamjung districts of Nepal were evaluated at Khumaltar, Lalitpur, Nepal during 2010. The plot size was 1m2 and there were five samples. Days to heading and days to maturity varied from 33-56 and 59 to 87 days after germination respectively. Similarly, flag leaf length/breadth ratio, flag leaf sheath length, ligule length, peduncle length, peduncle exertion and plant height varied from 3.84-10.90, 5.47-9.84 cm, 0.1-0.2 mm, 10-22.57 cm, 2.7-13.58 cm and 41.67-120 cm, respectively. Fruit and apiculus color varied from straw to black. All accessions were actively growing with very slight lodging. Similarly, the thousand grain weight varies from 1.064 g to 2.172 g. This variation is useful in foxtail millet breeding program. Similarly, the significant correlation between thousand kernel weight and total basal tiller (r=-0.975) showed that foxtail millet lines with low tillering ability is better for yield enhancement. DOI: http://dx.doi.org/10.3126/ajn.v2i0.7528 Agronomy Journal of Nepal (Agron JN) Vol. 2: 2011 pp.133-138

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