scholarly journals Systemic Colonization and Expression of Disease Symptoms on Bittersweet Nightshade (Solanum dulcamara) Infected with a GFP-Tagged Dickeya solani IPO2222 (IPO2254)

Plant Disease ◽  
2018 ◽  
Vol 102 (3) ◽  
pp. 619-627 ◽  
Author(s):  
Jakub Fikowicz-Krosko ◽  
Robert Czajkowski
Keyword(s):  
Vascular Tissue ◽  
Long Distance ◽  
Solanum Dulcamara ◽  
Disease Symptoms ◽  
Stem Base ◽  
In Vitro Plants ◽  
Dickeya Solani ◽  
Bittersweet Nightshade ◽  
Compost Soil

Colonization of Solanum dulcamara (bittersweet nightshade) plants by a GFP-tagged Dickeya solani type strain IPO2222 (IPO2254) was investigated by selective plating and epifluorescence stereomicroscopy (ESM), using in vitro plants and plants grown in compost soil. Replicated experiments were carried out in a growth chamber and the progress of infection and disease symptoms on tissue of the cultured plants, following leaf- and stem-base inoculations with bacteria, was evaluated. Microscopy observations were confirmed by spread-plating dilutions of plant extracts onto agar medium directly after the harvest. In experiments where the stem base of in vitro plants inoculated with a range of inocula of D. solani (104 to 108 colony forming units [cfu] ml−1) was examined at 14 days post infection (dpi), blackleg-like symptoms developed in more than 80% plants together with a reduction of the plant fitness (disease symptoms, weight, height, and appearance). In leaf-inoculated plants at 14 dpi, 15% of the plants exhibited severe blackleg-like symptoms. In detached S. dulcamara leaf assays, IPO2254 survived on the adaxial surface for 14 days at populations of 106 cfu per leaf. Thirty days after stem inoculation of plants grown in compost soil in pots, up to 104 cfu g−1 of GFP-tagged D. solani were found inside the stems. D. solani were detected inside the vascular tissue (xylem vessels) of stems, in the pith tissue in roots, and on the internal surface of the stem hollow. The implications of S. dulcamara infection by D. solani for the long-distance dispersal of the bacterial inoculum are discussed.

Influence of salicylic acid on organogenesis of in vitro plants Fragaria ananassa Duch.

2016 ◽  
Vol 48 (1) ◽  
pp. 26-33
Author(s):  
O.V. Subin ◽  
◽  
M.D. Melnychuk ◽  
A.F. Likhanov ◽  
O.L. Klyachenko ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Fragaria Ananassa ◽  
In Vitro Plants

Surface Modification by Nanobiomaterials for Vascular Tissue Engineering Applications

2020 ◽  
Vol 27 (10) ◽  
pp. 1634-1646 ◽  
Author(s):  
Huey-Shan Hung ◽  
Shan-hui Hsu
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Thrombus Formation ◽  
Vascular Grafts ◽  
Vascular Tissue Engineering ◽  
Great Success ◽  
Natural Polymers ◽  
Vascular Biomaterials

Treatment of cardiovascular disease has achieved great success using artificial implants, particularly synthetic-polymer made grafts. However, thrombus formation and restenosis are the current clinical problems need to be conquered. New biomaterials, modifying the surface of synthetic vascular grafts, have been created to improve long-term patency for the better hemocompatibility. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering. Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular tissues. To overcome the thrombogenesis and promote the endothelialization effect, vascular biomaterials employing nanotopography are more bio-mimic to the native tissue made and have been engineered by various approaches such as prepared as a simple surface coating on the vascular biomaterials. It has now become an important and interesting field to find novel approaches to better endothelization of vascular biomaterials. In this article, we focus to review the techniques with better potential improving endothelization and summarize for vascular biomaterial application. This review article will enable the development of biomaterials with a high degree of originality, innovative research on novel techniques for surface fabrication for vascular biomaterials application.

scholarly journals Plant-Derived Nano and Microvesicles for Human Health and Therapeutic Potential in Nanomedicine

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 498
Author(s):  
Mariaevelina Alfieri ◽  
Antonietta Leone ◽  
Alfredo Ambrosone
Keyword(s):  
Therapeutic Potential ◽  
Biological Activities ◽  
Plant Biotechnology ◽  
Bioactive Metabolites ◽  
Anticancer Activities ◽  
Long Distance ◽  
In Vivo Models ◽  
Therapeutic Tools

Plants produce different types of nano and micro-sized vesicles. Observed for the first time in the 60s, plant nano and microvesicles (PDVs) and their biological role have been inexplicably under investigated for a long time. Proteomic and metabolomic approaches revealed that PDVs carry numerous proteins with antifungal and antimicrobial activity, as well as bioactive metabolites with high pharmaceutical interest. PDVs have also been shown to be also involved in the intercellular transfer of small non-coding RNAs such as microRNAs, suggesting fascinating mechanisms of long-distance gene regulation and horizontal transfer of regulatory RNAs and inter-kingdom communications. High loading capacity, intrinsic biological activities, biocompatibility, and easy permeabilization in cell compartments make plant-derived vesicles excellent natural or bioengineered nanotools for biomedical applications. Growing evidence indicates that PDVs may exert anti-inflammatory, anti-oxidant, and anticancer activities in different in vitro and in vivo models. In addition, clinical trials are currently in progress to test the effectiveness of plant EVs in reducing insulin resistance and in preventing side effects of chemotherapy treatments. In this review, we concisely introduce PDVs, discuss shortly their most important biological and physiological roles in plants and provide clues on the use and the bioengineering of plant nano and microvesicles to develop innovative therapeutic tools in nanomedicine, able to encompass the current drawbacks in the delivery systems in nutraceutical and pharmaceutical technology. Finally, we predict that the advent of intense research efforts on PDVs may disclose new frontiers in plant biotechnology applied to nanomedicine.

scholarly journals Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications: Bench optimisation and feasibility in vivo testing

2021 ◽  
Vol 12 ◽  
pp. 204173142098752
Author(s):  
Nadiah S Sulaiman ◽  
Andrew R Bond ◽  
Vito D Bruno ◽  
John Joseph ◽  
Jason L Johnson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Vascular Tissue ◽  
Sodium Dodecyl ◽  
Host Cells ◽  
Replacement Model ◽  
In Vivo Testing ◽  
Vascular Scaffolds

Human saphenous vein (hSV) and synthetic grafts are commonly used conduits in vascular grafting, despite high failure rates. Decellularising hSVs (D-hSVs) to produce vascular scaffolds might be an effective alternative. We assessed the effectiveness of a detergent-based method using 0% to 1% sodium dodecyl sulphate (SDS) to decellularise hSV. Decellularisation effectiveness was measured in vitro by nuclear counting, DNA content, residual cell viability, extracellular matrix integrity and mechanical strength. Cytotoxicity was assessed on human and porcine cells. The most effective SDS concentration was used to prepare D-hSV grafts that underwent preliminary in vivo testing using a porcine carotid artery replacement model. Effective decellularisation was achieved with 0.01% SDS, and D-hSVs were biocompatible after seeding. In vivo xeno-transplantation confirmed excellent mechanical strength and biocompatibility with recruitment of host cells without mechanical failure, and a 50% patency rate at 4-weeks. We have developed a simple biocompatible methodology to effectively decellularise hSVs. This could enhance vascular tissue engineering toward future clinical applications.

Endothelium-dependent vasorelaxing activity of wine and other grape products

1993 ◽  
Vol 265 (2) ◽  
pp. H774-H778 ◽  
Author(s):  
D. F. Fitzpatrick ◽  
S. L. Hirschfield ◽  
R. G. Coffey
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Vascular Function ◽  
Vascular Tissue ◽  
Grape Skin ◽  
Grape Skins ◽  
Grape Juices ◽  
Patent Coronary Artery

Current interest in the presumed benefits of wine in protecting against coronary heart disease prompted us to investigate possible effects of various grape products on vascular function in vitro. Certain wines, grape juices, and grape skin extracts relaxed precontracted smooth muscle of intact rat aortic rings but had no effect on aortas in which the endothelium had been removed. Quercitin and tannic acid, compounds known to be present in grape skins, also produced endothelium-dependent relaxation; two other grape skin compounds, resveratrol and malvidin, did not relax the rings. Phenylephrine-induced contractions were attenuated by prior exposure of aortic rings to grape skin extracts. The extracts also increased guanosine 3',5'-cyclic monophosphate (cGMP) levels in intact vascular tissue, and both relaxation and the increase in cGMP were reversed by NG-monomethyl-L-arginine and NG-nitro-L-arginine, competitive inhibitors of the synthesis of the endothelium-derived relaxing factor, nitric oxide (NO). The vasorelaxation induced by grape products therefore appears to be mediated by the NO-cGMP pathway. If such responses occur in vivo, they could conceivably help to maintain a patent coronary artery and thereby possibly contribute to a reduced incidence of coronary heart disease.

Hemocompatibility evaluation of poly(glycerol-sebacate) in vitro for vascular tissue engineering

Biomaterials ◽  
2006 ◽  
Vol 27 (24) ◽  
pp. 4315-4324 ◽  
Author(s):  
Delara Motlagh ◽  
Jian Yang ◽  
Karen Y. Lui ◽  
Antonio R. Webb ◽  
Guillermo A. Ameer
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering

scholarly journals Effect of extracts from field and in vitro plants of Petiveria alliacea L. on plasmidial DNA

2014 ◽  
Vol 8 (35) ◽  
pp. 1101-1109 ◽  
Author(s):  
R. F. Gagliardi ◽  
◽  
B. O. Soares ◽  
M. B. N Oliveira ◽  
E. Mansur ◽  
...  
Keyword(s):  
In Vitro Plants ◽  
Petiveria Alliacea

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

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