Effects of Polyunsaturated Fatty Acids and Prostaglandins on Oocyte Maturation in a Marine Teleost, the European Sea Bass (Dicentrarchus labrax)1

A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09T, was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum ‘Bacteroidetes’). Sequence similarities between the isolate and type strains of other members of the genus were 93.1–97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C15 : 0 (24.8 %), iso-C15 : 0 3-OH (18.0 %), anteiso-C15 : 0 (8.1 %), C15 : 1ω6c (6.9 %) and iso-C15 : 1 (6.2 %). Genotypic and phenotypic data indicate that strain 35/09T should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09T ( = CECT 7612T = NCIMB 14598T).

Download Full-text

Determination of Hematological Status as Health Indicator of European Sea Bass (Dicentrarchus labrax L.) Fed Different Dietary Fatty Acids

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v5i4.445-450.1106 ◽

2017 ◽

Vol 5 (4) ◽

pp. 445

Author(s):

Aysel Şahan ◽

Hatice Asuman Yılmaz ◽

Orhan Tufan Erdoğan

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Canola Oil ◽

Hematological Parameters ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Cottonseed Oil ◽

Dietary Fatty Acids ◽

European Sea Bass

In this study, the European Sea Bass (Dicentrarchus labrax) were fed rich canola oil which is containing monounsaturated fatty acid (MUFA) and rich cottonseed oil which is containing n-6 polyunsaturated fatty acid (PUFA) and the effects of these feeds on some hematological parameters were investigated. Experimental fish were fed two times daily with 100% fish oil (FO-control), 100% cottonseed oil (CSO), 100% canola oil (CO) and 50% CSO - 50% CO (CSO50-CO50) for 162 days. End of the experiment, total erythrocyte (RBC) amount was highest in the CSO group 269.0 x (104 mm3 -1) compared to all other groups. Leukocyte (WBC) 96.280 x (103 mm3 -1), monocyte, lymphocyte and neutrophil amounts showed significant increases in only CSO group. Therefore, in terms of fish health and welfare, the exclusive use of cottonseed oil without mixing with the other vegetable oils in sea bass diet formulation can be a viable alternative to fish oil since it has the most suitable fatty acids.

Download Full-text

Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)

Journal of Nutritional Science ◽

10.1017/jns.2015.6 ◽

2015 ◽

Vol 4 ◽

Cited By ~ 13

Author(s):

Daniel Montero ◽

Genciana Terova ◽

Simona Rimoldi ◽

Lluis Tort ◽

Davinia Negrin ◽

...

Keyword(s):

Fatty Acids ◽

Regulatory Protein ◽

Dicentrarchus Labrax ◽

Head Kidney ◽

Sea Bass ◽

Dietary Fatty Acids ◽

Acth Stimulation ◽

European Sea Bass ◽

Stress Related Genes ◽

Steroidogenic Genes

AbstractDietary fatty acids have been shown to exert a clear effect on the stress response, modulating the release of cortisol. The role of fatty acids on the expression of steroidogenic genes has been described in mammals, but little is known in fish. The effect of different fatty acids on the release of cortisol and expression of stress-related genes of European sea bass (Dicentrarchus labrax) head kidney, induced by a pulse of adenocorticotrophin hormone (ACTH), was studied. Tissue was maintained in superfusion with 60 min of incubation with EPA, DHA, arachidonic acid (ARA), linoleic acid or α-linolenic acid (ALA) during 490 min. Cortisol was measured by RIA. The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real-time RT-PCR. There was an effect of the type of fatty acid on the ACTH-induced release of cortisol, values from ALA treatment being elevated within all of the experimental period. The expression of some steroidogenic genes, such as the steroidogenic acute regulatory protein (StAR) and c-fos, were affected by fatty acids, ALA increasing the expression of StAR after 1 h of ACTH stimulation whereas DHA, ARA and ALA increased the expression of c-fos after 20 min. ARA increased expression of the 11β-hydroxylase gene. Expression of heat shock protein 70 (HSP70) was increased in all the experimental treatments except for ARA. Results corroborate previous studies of the effect of different fatty acids on the release of cortisol in marine fish and demonstrate that those effects are mediated by alteration of the expression of steroidogenic genes.

Download Full-text

Intake of high levels of vitamin A and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in European sea bass (Dicentrarchus labrax)

British Journal Of Nutrition ◽

10.1079/bjn20051668 ◽

2006 ◽

Vol 95 (4) ◽

pp. 677-687 ◽

Cited By ~ 76

Author(s):

Laure A. N. Villeneuve ◽

Enric Gisbert ◽

Jacques Moriceau ◽

Chantal L. Cahu ◽

José L. Zambonino Infante

Keyword(s):

Fatty Acids ◽

Vitamin A ◽

Fish Larvae ◽

Control Diet ◽

Juvenile Fish ◽

Dicentrarchus Labrax ◽

Subsequent Development ◽

Sea Bass ◽

Hypervitaminosis A ◽

European Sea Bass

The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13d post-hatching (dph); period B: from 13 to 18dph; period C: from 18 to 23dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades, resulting in two different kinds of malformation. Hypervitaminosis A delayed development, reducing the number of vertebral segments and disturbing bone formation in the cephalic region. These malformations were correlated to an upregulation of retinoic acid receptor γ, retinoid X receptor (RXR) α and bone morphogenetic protein (BMP)4. An excess of PUFA accelerated the osteoblast differentiation process through the upregulation of RXRα and BMP4, leading to a supernumerary vertebra. These results suggest that the composition of diets devoted to marine fish larvae has a particularly determining effect before 13dph on the subsequent development of larvae and juvenile fish.

Download Full-text