scholarly journals Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)

2015 ◽  
Vol 4 ◽  
Author(s):  
Daniel Montero ◽  
Genciana Terova ◽  
Simona Rimoldi ◽  
Lluis Tort ◽  
Davinia Negrin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Regulatory Protein ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Sea Bass ◽  
Dietary Fatty Acids ◽  
Acth Stimulation ◽  
European Sea Bass ◽  
Stress Related Genes ◽  
Steroidogenic Genes

AbstractDietary fatty acids have been shown to exert a clear effect on the stress response, modulating the release of cortisol. The role of fatty acids on the expression of steroidogenic genes has been described in mammals, but little is known in fish. The effect of different fatty acids on the release of cortisol and expression of stress-related genes of European sea bass (Dicentrarchus labrax) head kidney, induced by a pulse of adenocorticotrophin hormone (ACTH), was studied. Tissue was maintained in superfusion with 60 min of incubation with EPA, DHA, arachidonic acid (ARA), linoleic acid or α-linolenic acid (ALA) during 490 min. Cortisol was measured by RIA. The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real-time RT-PCR. There was an effect of the type of fatty acid on the ACTH-induced release of cortisol, values from ALA treatment being elevated within all of the experimental period. The expression of some steroidogenic genes, such as the steroidogenic acute regulatory protein (StAR) and c-fos, were affected by fatty acids, ALA increasing the expression of StAR after 1 h of ACTH stimulation whereas DHA, ARA and ALA increased the expression of c-fos after 20 min. ARA increased expression of the 11β-hydroxylase gene. Expression of heat shock protein 70 (HSP70) was increased in all the experimental treatments except for ARA. Results corroborate previous studies of the effect of different fatty acids on the release of cortisol in marine fish and demonstrate that those effects are mediated by alteration of the expression of steroidogenic genes.

scholarly journals Determination of Hematological Status as Health Indicator of European Sea Bass (Dicentrarchus labrax L.) Fed Different Dietary Fatty Acids

2017 ◽  
Vol 5 (4) ◽  
pp. 445
Author(s):  
Aysel Şahan ◽  
Hatice Asuman Yılmaz ◽  
Orhan Tufan Erdoğan
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Canola Oil ◽  
Hematological Parameters ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Cottonseed Oil ◽  
Dietary Fatty Acids ◽  
European Sea Bass

In this study, the European Sea Bass (Dicentrarchus labrax) were fed rich canola oil which is containing monounsaturated fatty acid (MUFA) and rich cottonseed oil which is containing n-6 polyunsaturated fatty acid (PUFA) and the effects of these feeds on some hematological parameters were investigated. Experimental fish were fed two times daily with 100% fish oil (FO-control), 100% cottonseed oil (CSO), 100% canola oil (CO) and 50% CSO - 50% CO (CSO50-CO50) for 162 days. End of the experiment, total erythrocyte (RBC) amount was highest in the CSO group 269.0 x (104 mm3 -1) compared to all other groups. Leukocyte (WBC) 96.280 x (103 mm3 -1), monocyte, lymphocyte and neutrophil amounts showed significant increases in only CSO group. Therefore, in terms of fish health and welfare, the exclusive use of cottonseed oil without mixing with the other vegetable oils in sea bass diet formulation can be a viable alternative to fish oil since it has the most suitable fatty acids.

scholarly journals Potential Involvement of lncRNAs in the Modulation of the Transcriptome Response to Nodavirus Challenge in European Sea Bass (Dicentrarchus labrax L.)

Biology ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 165
Author(s):  
Patricia Pereiro ◽  
Raquel Lama ◽  
Rebeca Moreira ◽  
Valentina Valenzuela-Muñoz ◽  
Cristian Gallardo-Escárate ◽  
...  
Keyword(s):  
Immune Response ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Enrichment Analysis ◽  
Sea Bass ◽  
Integrated Analysis ◽  
European Sea Bass ◽  
Commercial Species ◽  
Number Of Publications ◽  
Systematic Identification

Long noncoding RNAs (lncRNAs) are being increasingly recognised as key modulators of various biological mechanisms, including the immune response. Although investigations in teleosts are still lagging behind those conducted in mammals, current research indicates that lncRNAs play a pivotal role in the response of fish to a variety of pathogens. During the last several years, interest in lncRNAs has increased considerably, and a small but notable number of publications have reported the modulation of the lncRNA profile in some fish species after pathogen challenge. This study was the first to identify lncRNAs in the commercial species European sea bass. A total of 12,158 potential lncRNAs were detected in the head kidney and brain. We found that some lncRNAs were not common for both tissues, and these lncRNAs were located near coding genes that are primarily involved in tissue-specific processes, reflecting a degree of cellular specialisation in the synthesis of lncRNAs. Moreover, lncRNA modulation was analysed in both tissues at 24 and 72 h after infection with nodavirus. Enrichment analysis of the neighbouring coding genes of the modulated lncRNAs revealed many terms related to the immune response and viral infectivity but also related to the stress response. An integrated analysis of the lncRNAs and coding genes showed a strong correlation between the expression of the lncRNAs and their flanking coding genes. Our study represents the first systematic identification of lncRNAs in European sea bass and provides evidence regarding the involvement of these lncRNAs in the response to nodavirus.

scholarly journals Genome-wide epigenomic stress response in the European sea bass (Dicentrarchus labrax, L.)

2020 ◽  
Author(s):  
Madoka V. KRICK ◽  
Erick DESMARAIS ◽  
Athanasios SAMARAS ◽  
Elise GUERET ◽  
Arkadios DIMITROGLOU ◽  
...  
Keyword(s):  
Stress Response ◽  
Cytosine Methylation ◽  
Acute Stress ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Blood Parameter ◽  
European Sea Bass ◽  
Genome Wide ◽  
Stress Related Genes ◽  
The Brain

Abstract Background: While the stress response inspired genome-wide epigenetic studies in vertebrate models, it remains mostly ignored in fish. We modified the epiGBS (epiGenotyping By sequencing) technique to explore changes in genome-wide cytosine methylation to a repeated acute stress challenge in the nucleated red blood cells (RBCs) of the European sea bass (Dicentrarchus labrax). This species is widely studied in both the natural and farmed environments, including issues regarding health and welfare.Results: We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Minor changes in RBC methylome appear to manifest after the stress challenge. Only, fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct stress-related genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-stress individuals. However, literature surveys indicated that 38 of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, appear especially relevant to integrate a centrally produced stress response.Conclusion: By putting forward DMCs associated to stress-related genes, we show that minimally invasive RBCs deserve more attention to investigate the epigenetic response to stress and components of the stress response without sacrificing fish. In parallel to blood parameter measurements (e.g. cortisol, glucose levels), and other molecular approaches (e.g. gene expression variation), features of the epigenetic landscape may offer new opportunities for biomonitoring components of the stress response in fish.

scholarly journals Ceratothoa oestroides Infection in European Sea Bass: Revealing a Long Misunderstood Relationship

2021 ◽  
Vol 12 ◽  
Author(s):  
M. Carla Piazzon ◽  
Ivona Mladineo ◽  
Ron P. Dirks ◽  
Elena Santidrián Yebra-Pimentel ◽  
Jerko Hrabar ◽  
...  
Keyword(s):  
Feeding Strategy ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Sea Bass ◽  
Growth Delay ◽  
European Sea Bass ◽  
Feeding Site ◽  
Collagen Formation ◽  
North East ◽  
The North

Ceratothoa oestroides (Cymothoidea, Isopoda) is a generalist crustacean parasite that negatively affects the economic sustainability of European sea bass (Dicentrarchus labrax) aquaculture in the North-East Mediterranean. While mortalities are observed in fry and fingerlings, infection in juvenile and adult fish result in approximately 20% growth delay. A transcriptomic analysis (PCR array, RNA-Seq) was performed on organs (tongue, spleen, head kidney, and liver) from infected vs. Ceratothoa-free sea bass fingerlings. Activation of local and systemic immune responses was detected, particularly in the spleen, characterized by the upregulation of cytokines (also in the tongue), a general reshaping of the immunoglobulin (Ig) response and suppression of T-cell mediated responses. Interestingly, starvation and iron transport and metabolism genes were strongly downregulated, suggesting that the parasite feeding strategy is not likely hematophagous. The regulation of genes related to growth impairment and starvation supported the growth delay observed in infected animals. Most differentially expressed (DE) transcripts were exclusive of a specific organ; however, only in the tongue, the difference between infected and uninfected fish was significant. At the attachment/feeding site, the pathways involved in muscle contraction and intercellular junction were the most upregulated, whereas the pathways involved in fibrosis (extracellular matrix organization, collagen formation, and biosynthesis) were downregulated. These results suggest that parasite-inflicted damage is successfully mitigated by the host and characterized by regenerative processes that prevail over the reparative ones.

scholarly journals Identification and Regulation of Interleukin-17 (IL-17) Family Ligands in the Teleost Fish European Sea Bass

2020 ◽  
Vol 21 (7) ◽  
pp. 2439 ◽  
Author(s):  
Carmen González-Fernández ◽  
Elena Chaves-Pozo ◽  
Alberto Cuesta
Keyword(s):  
Regulation Of Gene Expression ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Sea Bass ◽  
Interleukin 17 ◽  
Target Tissue ◽  
Mrna Levels ◽  
European Sea Bass ◽  
The Brain

Interleukin-17 (IL-17) cytokine comprises a family of six ligands in mammals with proinflammatory functions, having an important role in autoimmune disorders and against bacterial, viral, and fungal pathogens. While IL-17A and IL-17F ligands are mainly produced by Th cells (Th17 cells), the rest of the ligands are expressed by other immune and non-immune cells and have different functions. The identification of IL-17 ligands in fish has revealed the presence of six members, counterparts to mammalian ones, and a teleost-specific form, the fish IL-17N. However, tissue distribution, the regulation of gene expression, and scarce bioactivity assays point to similar functions compared to mammalian ones, though this yet to be investigated and confirmed. Thus, we have identified seven IL-17 ligands in the teleost European sea bass (Dicentrarchus labrax), for the first time, corresponding to IL-17A/F1, IL-17A/F2, IL-17A/F3, IL-17C1, IL-17C2, IL-17D, and IL-17N, according to the predicted protein sequences and phylogenetic analysis. They are constitutively and widely transcribed in sea bass tissues, with some of them being mainly expressed in the thymus, brain or intestine. Upon in vitro stimulation of head-kidney leucocytes, the mRNA levels of all sea bass IL-17 ligands were up-regulated by phytohemagglutinin treatment, a well-known T cell mitogen, suggesting a major expression in T lymphocytes. By contrast, the infection of sea bass juveniles with nodavirus (NNV), a very pathogenic virus for this fish species, resulted in the up-regulation of the transcription of IL-17C1 in the head-kidney and of IL-17C1 and IL-17D in the brain, the target tissue for NNV replication. By contrast, NNV infection led to a down-regulated transcription of IL-17A/F1, IL-17A/F2, IL-17C1, IL-17C2, and IL-17D in the head-kidney and of IL-17A/F1 and IL-17A/F3 in the brain. The data are discussed accordingly with the IL-17 ligand expression and the immune response under the different situations tested.

scholarly journals Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from European sea bass

2012 ◽  
Vol 62 (2) ◽  
pp. 425-429 ◽  
Author(s):  
Maximino Piñeiro-Vidal ◽  
Daniel Gijón ◽  
Carles Zarza ◽  
Ysabel Santos
Keyword(s):  
Fatty Acids ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Rrna Gene ◽  
European Sea Bass ◽  
Phenotypic Data ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Total Fatty Acids ◽  
Type Strains

A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09T, was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum ‘Bacteroidetes’). Sequence similarities between the isolate and type strains of other members of the genus were 93.1–97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C15 : 0 (24.8 %), iso-C15 : 0 3-OH (18.0 %), anteiso-C15 : 0 (8.1 %), C15 : 1ω6c (6.9 %) and iso-C15 : 1 (6.2 %). Genotypic and phenotypic data indicate that strain 35/09T should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09T ( = CECT 7612T = NCIMB 14598T).

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