scholarly journals Incorporation of dietary fatty acid in European sea bass (Dicentrarchus labrax) — A methodological approach evidencing losses of highly unsaturated fatty acids

Aquaculture ◽  
2007 ◽  
Vol 263 (1-4) ◽  
pp. 227-237 ◽  
Author(s):  
J.H. Robin ◽  
A. Skalli
Author(s):  
Aysel Şahan ◽  
Hatice Asuman Yılmaz ◽  
Orhan Tufan Erdoğan

In this study, the European Sea Bass (Dicentrarchus labrax) were fed rich canola oil which is containing monounsaturated fatty acid (MUFA) and rich cottonseed oil which is containing n-6 polyunsaturated fatty acid (PUFA) and the effects of these feeds on some hematological parameters were investigated. Experimental fish were fed two times daily with 100% fish oil (FO-control), 100% cottonseed oil (CSO), 100% canola oil (CO) and 50% CSO - 50% CO (CSO50-CO50) for 162 days. End of the experiment, total erythrocyte (RBC) amount was highest in the CSO group 269.0 x (104 mm3 -1) compared to all other groups. Leukocyte (WBC) 96.280 x (103 mm3 -1), monocyte, lymphocyte and neutrophil amounts showed significant increases in only CSO group. Therefore, in terms of fish health and welfare, the exclusive use of cottonseed oil without mixing with the other vegetable oils in sea bass diet formulation can be a viable alternative to fish oil since it has the most suitable fatty acids.


2020 ◽  
Vol 37 (1) ◽  
pp. 43-51
Author(s):  
Yılmaz Uçar ◽  
Fatih Özoğul ◽  
Mustafa Durmuş ◽  
Ali Rıza Kösşker ◽  
Yeşim Özoğul

In this study the effects of nisin used at different concentrations (0.2%, 0.4% and 0.8% w/v) on the fatty acids profile of vacuum-packed sea bass (Dicentrarchus labrax) fillets were investigated during the 18 day of cold storage period. As a result of the fatty acid analysis throughout storage high concentration saturated fatty acids (SFA) were determined as myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0) and high concentrations of monounsaturated fatty acids (MUFA) were determined as palmitoleic acid (C16:1), oleic acid (C18:1n9), waxenic acid (C18:1n7), eicozenoic acid (C20:1n9). Polyunsaturated fatty acids (PUFA) which were determined at high concentration were linoleic acid (C18:2n6), linolenic acid (C18:3n3), eicosapentaenoic acid (EPA, C20:5n3) and decosahexaenoic acid (DHA, C22:6n3). At the end of the storage period, saturated fatty acid levels in the control and nisin treatment groups increased and monounsaturated and polyunsaturated fatty acid levels generally decreased. This is thought to be related to the transformation of especially unsaturated fatty acids to peroxides and aldehydes, ketones and alcohols as a result of auto-oxidation reactions. In the present study, lowest polyunsaturated fatty acid levels were found in the control group on the last day of the storage period. Among treatment groups, 0.8% nisin group gave the highest PUFA content. It was thought that the application of nisin delay the auto-oxidation reactions of unsaturated fatty acids. It was thought that the type of oil used to prepare nanoemulsions can have a positive effect on fatty acid compositions. As a result, it is thought that the nisin depending on the dose used can have a positive effect on fatty acid compositions of seabass fillets.


2002 ◽  
Vol 364 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Sabine D'ANDREA ◽  
Hervé GUILLOU ◽  
Sophie JAN ◽  
Daniel CATHELINE ◽  
Jean-Noël THIBAULT ◽  
...  

The recently cloned Δ6-desaturase is known to catalyse the first step in very-long-chain polyunsaturated fatty acid biosynthesis, i.e. the desaturation of linoleic and α-linolenic acids. The hypothesis that this enzyme could also catalyse the terminal desaturation step, i.e. the desaturation of 24-carbon highly unsaturated fatty acids, has never been elucidated. To test this hypothesis, the activity of rat Δ6-desaturase expressed in COS-7 cells was investigated. Recombinant Δ6-desaturase expression was analysed by Western blot, revealing a single band at 45kDa. The putative involvement of this enzyme in the Δ6-desaturation of C24:5n-3 to C24:6n-3 was measured by incubating transfected cells with C22:5n-3. Whereas both transfected and non-transfected COS-7 cells were able to synthesize C24:5n-3 by elongation of C22:5n-3, only cells expressing Δ6-desaturase were also able to produce C24:6n-3. In addition, Δ6-desaturation of [1-14C]C24:5n-3 was assayed invitro in homogenates from COS-7 cells expressing Δ6-desaturase or not, showing that Δ6-desaturase catalyses the conversion of C24:5n-3 to C24:6n-3. Evidence is therefore presented that the same rat Δ6-desaturase catalyses not only the conversion of C18:3n-3 to C18:4n-3, but also the conversion of C24:5n-3 to C24:6n-3. A similar mechanism in the n-6 series is strongly suggested.


2014 ◽  
Vol 47 (4) ◽  
pp. 1067-1074 ◽  
Author(s):  
Gabriel Fernando Ballester-Lozano ◽  
Laura Benedito-Palos ◽  
Mónica Mingarro ◽  
Juan Carlos Navarro ◽  
Jaume Pérez-Sánchez

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