scholarly journals The effects of ethanol extract of peros Punica granatum L. peel on the oxidative damage induced by diabet in rats

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Ali Dogan ÖMÜR ◽  
Betül Dogan Apaydın Yıldırım
Keyword(s):  
Oxidative Damage ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Punica Granatum L

Protective role of Punica granatum L. peel extract against oxidative damage in experimental diabetic rats

2010 ◽  
Vol 45 (4) ◽  
pp. 581-585 ◽  
Author(s):  
Osama Y. Althunibat ◽  
Ahmed H. Al-Mustafa ◽  
Khaled Tarawneh ◽  
Khaled M. Khleifat ◽  
B.H. Ridzwan ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Punica Granatum ◽  
Protective Role ◽  
Diabetic Rats ◽  
Punica Granatum L ◽  
Peel Extract

scholarly journals Optimization of Fermentation Process of Pomegranate (Punica Granatum L.) Peel and Schisandra Chinensis (Turcz.) Baill and the Biological Activities of Fermentation Broth: Antioxidant Activity and Protective Effect Against H2O2-induced Oxidative Damage in HaCaT Cells

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3432
Author(s):  
Hui-Min Liu ◽  
Peng-Fei Xu ◽  
Ming-Yan Cheng ◽  
Sheng-Nan Lei ◽  
Qing-Lei Liu ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Protective Effect ◽  
Fermentation Process ◽  
Fermentation Broth ◽  
Punica Granatum ◽  
Cell Counting ◽  
Hacat Cells ◽  
Schisandra Chinensis ◽  
Fermentation Time ◽  
Punica Granatum L

In this study, the lactobacillus fermentation process of pomegranate (Punica granatum L.) peel and Schisandra chinensis (Turcz.) Baill (PP&SC) was optimized by using the response surface method (RSM) coupled with a Box-Behnken design. The optimum fermentation condition with the maximal yield of ellagic acid (99.49 ± 0.47 mg/g) was as follows: 1:1 (w:w) ratio of pomegranate peel to Schisandra chinensis, 1% (v:v) of strains with a 1:1 (v:v) ratio of Lactobacillus Plantarum to Streptococcus Thermophilus, a 37 °C fermentation temperature, 33 h of fermentation time, 1:20 (g:mL) of a solid–liquid ratio and 3 g/100 mL of a glucose dosage. Under these conditions, the achieved fermentation broth (FB) showed stronger free radical scavenging abilities than the water extract (WE) against the ABTS+, DPPH, OH− and O2− radicals. The cytotoxicity and the protective effect of FB on the intracellular ROS level in HaCaT cells were further detected by the Cell Counting Kit-8 (CCK-8) assay. The results showed that FB had no significant cytotoxicity toward HaCaT cells when its content was no more than 8 mg/mL. The FB with a concentration of 8 mg/mL had a good protective effect against oxidative damage, which can effectively reduce the ROS level to 125.94% ± 13.46% (p < 0.001) compared with 294.49% ± 11.54% of the control group in H2O2-damaged HaCaT cells. The outstanding antioxidant ability and protective effect against H2O2-induced oxidative damage in HaCaT cells promote the potential for the FB of PP&SC as a functional raw material of cosmetics.

scholarly journals ANTIDIARRHEAL ACTIVITY TEST OF ETHANOLIC EXTRACT OF WHITE POMEGRANATE PEEL (PUNICA GRANATUM L.) IN MALE WHITE MICE (MUS MUSCULUS), SWISS WEBSTER LINE USING THE INTESTINAL TRANSIT METHOD

2021 ◽  
pp. 124-128
Author(s):  
NUR RAHAYUNINGSIH ◽  
ROFFY OKTAVIAN ◽  
TITA NOFIANTI
Keyword(s):  
White Male ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Positive Control ◽  
Intestinal Transit ◽  
Pomegranate Peel ◽  
Antidiarrheal Activity ◽  
Punica Granatum L ◽  
Transit Method

Objective: Diarrheal disease and its complications remain a major cause of morbidity and mortality in children, especially in developing countries. It is usually a symptom of an infection in the intestinal tract, which can be caused by a variety of bacterial, viral, parasitic or organisms. The purpose of this study was to determine the activity and dose of white pomegranate peel (Punica granatum L.) ethanol extract as an antidiarrheal in white male mice using the intestinal transit method. Methods: Mice were grouped into 5 groups: negative control (Na CMC 1 %), positive control (loperamide HCl 0.0104 mg/20 g mice BW), and pomegranate peel ethanol extract test group 1, 2, and 3 (dose of 16, 32, and 64 mg/20 g mice BW). The length of the intestine that the ink marker traversed from the pylorus to the end (which is black) was measured using a ruler. Results: Based on statistical analysis, there were significant differences between all groups (<0.05). The highest antidiarrheal activity was in the ethanolic extract of pomegranate peel at a dose of 64 mg/20 g mice BW with an inhibition percentage of 36.44% and higher than the positive control (29.81%). The inhibition percentage was also resulted by dose 1 and 2 (12.46% and 29.53%, respectively). Conclusion: From these results show a correlation that the higher the extract dose, the higher the antidiarrheal potential.

scholarly journals GASTRIC ANTIULCER AND ULCER HEALING EFFECTS OF PUNICA GRANATUM L. PEEL EXTRACT IN RATS: ROLE OF OFFENSIVE AND DEFENSIVE MUCOSAL FACTORS AND OXIDATIVE STRESS

2017 ◽  
Vol 9 (5) ◽  
pp. 6
Author(s):  
Indal Chauhan ◽  
Ankita Sharma ◽  
Mayank Gangwar ◽  
Manish Kumar Gautam ◽  
Amit Singh ◽  
...  
Keyword(s):  
Ulcer Healing ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Mucosal Damage ◽  
Gastric Ulcers ◽  
Pylorus Ligation ◽  
High Antioxidant Activity ◽  
Cold Restraint Stress ◽  
Punica Granatum L

Objective: The present work incorporates the study of gastric antiulcer and ulcer healing effects of dried Punica granatum (PG) peel 50% ethanol extract (PGE) in rats.Methods: PGE (100 mg/kg) was administered orally once daily to rats either before or after induction of gastric ulcers (GU) for 7 d. Antiulcer effects of PGE were seen against acute GU, induced by pylorus ligation (PL), cold restraint stress (CRS), aspirin and ethanol while, ulcer healing in acetic acid (AA)-induced chronic GU in rats. Ulcer index (UI), gastric juice volume, acid-pepsin and mucin secretions and gastric mucosal glycoproteins, free radicals (LPO and NO) and antioxidants (SOD and GSH) were estimated.Results: PGE showed a decrease in UI in all GU models (45.6 to 79.7%, P<0.05 to P<0.001) indicating both protective and healing effects. PGE showed little or no effects on volume, acid-pepsin concentration and output but increased mucin secretion (55.1%, P<0.05) and mucosal glycoproteins (35.7%, P<0.05) in PL rats. CRS rats showed an increase in LPO and NO (48.4 to 58.3%, P<0.01) and SOD (21.8%, P<0.01) but decrease in GSH and CAT (33.1 to 44.8%, P<0.01 to P<0.001) compared with unstressed rats. PGE-treated CRS rats showed a decrease in LPO and NO (44.1 to 61.2, P<0.01 to P<0.001) and SOD (13.2%, P<0.01) and increase in GSH and CAT (43.8 to 48.7%, P<0.01 to P<0.001) compared with CRS rats.Conclusion: PGE seemed to have ulcer cytoprotective effects due to enhanced mucosal resistance and reduction in oxidative mucosal damage possibly via high antioxidant activity.

scholarly journals The potential of ethanol extract of white pomegranate leaves (Punica granatum L) as anti-bacterial

2020 ◽  
Vol 9 (2) ◽  
pp. 145-150
Author(s):  
Ni Made Susilawati ◽  
I Gede Putu Arnawa ◽  
Karol Octrisdey ◽  
Norma Tiku Kambuno
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Beta Lactamase ◽  
Leaf Extracts ◽  
Extended Spectrum Beta Lactamase ◽  
Punica Granatum L

Treatment of infections using penicillin-derived antibiotics such as methicillin has been found to cause antibiotic-resistant bacteria. This bacteria could produce a beta-lactamase enzyme to form a resistant strain. Research on antibacterial activity continues to develop. Pomegranate (Punica granatum L.) was one of the herbal plants whose fruit has long been used for the treatment and prevention of various diseases. This study aimed to determine the potential inhibition of white pomegranate leaf extracts (Punica granatum L.) on the growth of Gram-negative bacteria including Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) strain and Gram-positive bacteria Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus (MRSA) strain. White pomegranate leaf extract macerated with ethanol 96%, evaporated to obtain pure extracts made with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and with 100% tested with invitro diffusion method. It was found that the extract of white pomegranate leaves with 30% (10.00 ± 0.0) concentration was able to inhibit the growth of positive Gram bacteria strains MRSA  and the extract was unable to inhibit the growth of Escherichia coli bacteria strain ESBL.  

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT ACTIVITY AND ACUTE ORAL TOXICITY OF POMEGRANATE (PUNICA GRANATUM L.) FRUIT PEEL EXTRACT

2019 ◽  
pp. 7-14
Author(s):  
Hai Trieu Ly ◽  
Tuan Anh Vo ◽  
Viet Hong Phong Nguyen ◽  
Thi My Sa Pham ◽  
Bich Thao Lam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Punica Granatum ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Fruit Peel ◽  
Total Polyphenol ◽  
Punica Granatum L

Background: The natural antioxidants have an important role in the prevention of many diseases. The aim of study is to investigate phytochemical components, antioxidant activity and acute oral toxicity of Pomegranate (Punica granatum L.) fruit peel (PFP) extract. Materials and methods: Phytochemicals of PFP were determined by qualitative chemical tests, thin layer chromatography, total polyphenol and flavonoid contents. The PFP extract was evaluated for antioxidant activity by DPPH assay and MDA assay. In vivo acute oral toxicity test was conducted using Karber-Behrens method to determine LD50. Results: Results illustrated that PFP mainly contains flavonoids, alkaloids, tannins, triterpenes, saponins, and coumarins. PFP extract exhibited the total polyphenol and flavonoid contents with 189.97 mg gallic acid equivalent/g dry weight and 9.42 mg quercetin equivalent/g dry weight, respectively. The DPPH free radical scavenging and anti-lipid peroxidation activities of PFP extract were expressed with IC50 value of 4.80 μg/mL and 0.38 μg/ mL, sequentially. Simultaneously, the Dmax (the maximum dose administered to mice that no toxicity was observed) of PFP extract was determined to be 21.28 g/kg, equivalent to 35.64 g dried herb. Conclusion: The PFP extract is relatively safe and revealed high antioxidant activity. Key words: Punica granatum L.; polyphenols; flavonoids; gallic acid; quercetin; antioxidant activity; acute oral toxicity

Anti-Stress Effect of Punica granatum L. Extract against Sleep Deprivation-Induced Impairment

2016 ◽  
Vol 45 (11) ◽  
pp. 1533-1543
Author(s):  
Ju-Ryun Na ◽  
Sunoh Kim ◽  
Ara Jo ◽  
Donghyuck Bae ◽  
Kyo-Nyeo Oh ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Punica Granatum ◽  
Stress Effect ◽  
Punica Granatum L
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