scholarly journals ANTIDIARRHEAL ACTIVITY TEST OF ETHANOLIC EXTRACT OF WHITE POMEGRANATE PEEL (PUNICA GRANATUM L.) IN MALE WHITE MICE (MUS MUSCULUS), SWISS WEBSTER LINE USING THE INTESTINAL TRANSIT METHOD

2021 ◽  
pp. 124-128
Author(s):  
NUR RAHAYUNINGSIH ◽  
ROFFY OKTAVIAN ◽  
TITA NOFIANTI
Keyword(s):  
White Male ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Positive Control ◽  
Intestinal Transit ◽  
Pomegranate Peel ◽  
Antidiarrheal Activity ◽  
Punica Granatum L ◽  
Transit Method

Objective: Diarrheal disease and its complications remain a major cause of morbidity and mortality in children, especially in developing countries. It is usually a symptom of an infection in the intestinal tract, which can be caused by a variety of bacterial, viral, parasitic or organisms. The purpose of this study was to determine the activity and dose of white pomegranate peel (Punica granatum L.) ethanol extract as an antidiarrheal in white male mice using the intestinal transit method. Methods: Mice were grouped into 5 groups: negative control (Na CMC 1 %), positive control (loperamide HCl 0.0104 mg/20 g mice BW), and pomegranate peel ethanol extract test group 1, 2, and 3 (dose of 16, 32, and 64 mg/20 g mice BW). The length of the intestine that the ink marker traversed from the pylorus to the end (which is black) was measured using a ruler. Results: Based on statistical analysis, there were significant differences between all groups (<0.05). The highest antidiarrheal activity was in the ethanolic extract of pomegranate peel at a dose of 64 mg/20 g mice BW with an inhibition percentage of 36.44% and higher than the positive control (29.81%). The inhibition percentage was also resulted by dose 1 and 2 (12.46% and 29.53%, respectively). Conclusion: From these results show a correlation that the higher the extract dose, the higher the antidiarrheal potential.

scholarly journals DECOCTION OF POMEGRANATE (PUNICA GRANATUM L.) PEEL AS AN ANTHELMINTIC AGAINST TAENIA SAGINATA

2021 ◽  
pp. 129-131
Author(s):  
NYI MEKAR SAPTARINI ◽  
RESMI MUSTARICHIE
Keyword(s):  
Anthelmintic Activity ◽  
Punica Granatum ◽  
Positive Control ◽  
In Vitro Assay ◽  
Taenia Saginata ◽  
High Concentration ◽  
Pomegranate Peel ◽  
Vitro Assay ◽  
Punica Granatum L

Objective: The purpose of this study was to investigate the anthelmintic activity of pomegranate (Punica granatum L.) peel decoction against Taenia saginata. Methods: The in vitro assay was conducted by observing the motility of T. saginata, which is isolated from cattle’s gastrointestinal tract, in various concentrations of pomegranate peel decoction with albendazole as a positive control. Results: The results showed that the anthelmintic activity was dependent on decoction concentration and the duration of contact between decoction and nematode. Decoction at moderate concentration causes paralysis, while high concentration causes death. The 75% and 100% pomegranate peel decoction started to cause death at 240 min and 150 min after contact with T. saginata. Conclusion: It was concluded that pomegranate peel decoction has anthelmintic activity.

scholarly journals Evaluation of Antidiarrheal Activity of the Ethanol Extract Leucaena leucocephala (Lam) de Wit Seed

2020 ◽  
Vol 8 (A) ◽  
pp. 278-282
Author(s):  
Saddam Husein ◽  
Marline Nainggolan ◽  
Yuandani Yuandani ◽  
Irzal Fanany
Keyword(s):  
Leucaena Leucocephala ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Intestinal Transit ◽  
Dependent Manner ◽  
Stool Weight ◽  
Time Frequency ◽  
Antidiarrheal Activity ◽  
Ethanol Extracts

BACKGROUND: Leucaena leucocephala belongs to the Leguminosae/Fabaceae family. L. leucocephala seeds contain alkaloids, flavonoids, and tannins which according to the previous research have antidiarrhea activity. AIM: This study was investigate the antidiarrheal activity of the ethanol extracts of Leucaena leucocephala (Lam) de Wit seeds induced by oleum ricini and intestinal transit methods for rats. MATERIALS AND METHODS: L. leucocephala seeds were extracted by maceration with 80% ethanol. Evaluation of antidiarrheal extract activity was performed by induction of oleum ricini and intestinal transit methods. The extract at dose doses of 50, 100, 200, and 400 mg/kg BW was orally administered to the animals 1 h after induction by oleum ricini. Then diarrhea time, frequency, consistency, stool weight, and duration of diarrhea were observed every 30 min for 6 h. In determining the intestinal transit method, a percentage of the distance of the Chinese ink determined. This study was used positive control as Loperamide (1 mg/kg BW) and 0.5% Na-CMC as a negative control. RESULT: In diarrhea induced by castor oil, L. leucocephala seed extract at doses of 100, 200, and 400 mg/kg bw has been shown to significantly delay the onset of diarrhea, reduce diarrhea frequency, stool weight and duration of diarrhea compared with Na CMC as a negative control (p < 0.05). The extract at a dose of 400 mg/kg bw did not differ significantly from loperamide as positive control (p > 0.05). In this study, L. leucocephala extract reduced the distance traveled by Chinese ink in the intestine but only at a dose of 400 mg/kg bw which has a comparable activity with loperamide significantly . The antidiarrheal activity of extract showed at a dose dependent manner. CONCLUSION: The ethanol extract of L. leucocephala seeds has antidiarrheal activity which supports its use in folk medicines.

scholarly journals Ethanolic Extract Oitment of The Graptophylum Pictum L Griff Leaves as Analgesic agent to Male White Rat

2020 ◽  
Vol 3 (1) ◽  
pp. 32
Author(s):  
Setya Enti Rikomah ◽  
Ninsi Fitri
Keyword(s):  
White Male ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Good Effect ◽  
Test Results ◽  
Male Mice ◽  
Average Percentage ◽  
One Way Anova

<p>Graptophyllum pictum L Griff Leaves in the community are used as medicinal ingredients, one of which is for analgesics. Black pudding leaves contain secondary metabolite compounds that act as suspected analgesics, namely flavonoids. The ethanolic extract of black pudding leaves has been formulated into cream and ointment preparations. The purpose of this study was to determine the effectiveness of analgesic preparations of black pudding leaves (Graptophyllum pictum L. Griff) ethanol extract in white male mice. The effectiveness of analgesics was tested using the hotplate method. The test animals used were 25 white male mice which were divided into 5 groups: negative control (F0), positive control (Ichtiol® ointment), and black pudding leaf F1 (10%), F2 (15%) ethanol extract treatment group, and F3 (20%). Observation of stretching of mice was done after the feet of mice were induced and immediately given treatment. Observation of analgesic percentage in each mouse was carried out every 5 minutes for 60 minutes. The data obtained were processed and analyzed using one-way ANOVA and Duncan test with a 95% confidence level. One-way ANOVA test results showed that there were significant differences between treatment groups (0.003 &lt;0.05). The results of the average percentage of analgesic effectiveness of formula 2 have a good effect that is equal to 77.969%. Duncan's test results showed that the group that gave the best analgesic effectiveness was formula 2 (15%) comparable to positive control.</p><p><strong>Keywords: Graptophyllum pictum,  Analgesics, ointment, Hotplate.</strong><strong></strong></p>

Optimization of subcritical water extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel by response surface methodology

2017 ◽  
Vol 9 (32) ◽  
pp. 4647-4656 ◽  
Author(s):  
Linlin Yan ◽  
Yungang Cao ◽  
Guangyao Zheng
Keyword(s):  
Response Surface Methodology ◽  
Subcritical Water ◽  
Punica Granatum ◽  
Water Extraction ◽  
Phenolic Antioxidants ◽  
Subcritical Water Extraction ◽  
Extraction Technology ◽  
Pomegranate Peel ◽  
Efficient Extraction ◽  
Punica Granatum L

Subcritical water extraction (SWE), a ‘green’ and efficient extraction technology, was applied to extract phenolic antioxidants from pomegranate peel in this study.

scholarly journals PHYTOCHEMICAL EVALUATION OF PUNICA GRANATUM L. LEAF EXTRACT

2017 ◽  
Vol 9 (4) ◽  
pp. 14 ◽  
Author(s):  
P. Sreedevi ◽  
K. Vijayalakshmi ◽  
R. Venkateswari
Keyword(s):  
Metabolic Diseases ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Leaf Extracts ◽  
Bioactive Substances ◽  
Pharmaceutical Ingredients ◽  
Wide Range ◽  
Leaf Powder ◽  
Potent Drug ◽  
Punica Granatum L

Objective: This study was conducted to assess the phytochemical constituents in Punica granatum L. Leaf extracts (PGLE) using standard methods.Methods: The leaf powder was extracted using solvents namely aqueous, hydroalcohol, ethanol, ethyl acetate and n-hexane. Qualitative and Quantitative phytochemical screenings of PGLE were assessed by standard methods.Results: All the leaf extracts were positive for a wide range of bio-active compounds except n-hexane. The result has showed that the maximum amount of total phenols (394.16 mg/g DW of extract), total tannins (210.5 mg/g DW of extract), flavanoids (147.4 mg/g DW of extract) and total triterpenoids (112 mg/g DW of extract) were noted in ethanolic extract of P. granatum leaf (EPGL). The biological assay revealed that relevant amount of carbohydrate, protein, lipid and alkaloid in EPGL.Conclusion: The findings of this study concluded that the EPGL had potential bioactive substances that may be used as pharmaceutical ingredients for formulation of new or prospective potent drug to cure wide range of metabolic diseases.

scholarly journals Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

2020 ◽  
Vol 3 (2) ◽  
pp. 36-40
Author(s):  
Viki Ayu Intan Permatasari ◽  
Mutia Hariani Nurjanah ◽  
Wimbuh Tri Widodo
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
Traditional Medicines ◽  
Alternative Ingredients ◽  
High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

Aktivitas Antibakteri dari Ekstrak Etanol Ganoderma sp. Asal Pulau Lombok

2020 ◽  
Vol 6 (1) ◽  
pp. 22
Author(s):  
Adelina Oktaviani ◽  
Aida Muspiah ◽  
Faturrahman Faturrahman
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Large Diameter ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Gram Negative Bacteria ◽  
Activity Test

The use of antibiotics that are not according to the rules and antibiotics in the long term can cause resistance to bacteria. This study aims to determine the presence of antibacterial activity and the effect of increasing the concentration of ethanol extract of Ganoderma sp. against several test bacteria. Extract from Ganoderma sp. obtained by maceration method using ethanol 95% solvent. The extract concentrations used were 20%, 40%, 60% and 80%. This research was conducted using the wells method with ciprofloxacin as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition formed around the well. The results of the antibacterial activity test of ethanol extract Ganoderma sp. has greater inhibitory activity against gram-negative bacteria. The inhibitory activity of ethanolic extract of Ganoderma sp. on the growth of test bacteria increased with increasing concentration of the extract

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