Reduction of Gentamicin Bioassay Values in Sera with Elevated Inorganic Phosphorus, Uric Acid, Creatinine, and Alkaline Phosphatase Levels

1982 ◽  
Vol 4 (1) ◽  
pp. 95-98
Author(s):  
D. J. Flournoy
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Inorganic Phosphorus

Ultramicroscale Determination of Clinical Chemical Values for Blood during the First Four Days of Postnatal Life

1975 ◽  
Vol 21 (6) ◽  
pp. 746-750 ◽  
Author(s):  
Larissa deBaare ◽  
Jean Lewis ◽  
Helen Sing
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Total Protein ◽  
Inorganic Phosphorus ◽  
Postnatal Life ◽  
Total Proteins ◽  
Blood Constituents ◽  
Clinical Chemical ◽  
Term Newborns

Abstract Ultramicro procedures requiring 5-10 µl of serum or blood per analysis were used in determining blood constituents of healthy full-term newborns during the first four days of life. The resulting values appeared to be influenced by age, sex, and race. Values for total protein, albumin, urea nitrogen, and uric acid in serum decreased with time; serum inorganic phosphorus and whole-blood aldosaccharoses increased. Serum from females had higher values than that from males for total proteins, albumin, and inorganic phosphorus. The values for serum calcium and alkaline phosphatase were consistently higher in Negro than in white infants; values for uric acid were higher in the latter.

Serum-constituents analyses: effect of duration and temperature of storage of clotted blood.

1981 ◽  
Vol 27 (1) ◽  
pp. 35-38 ◽  
Author(s):  
T Ono ◽  
K Kitaguchi ◽  
M Takehara ◽  
M Shiiba ◽  
K Hayami
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Zinc Sulfate ◽  
Leucine Aminopeptidase ◽  
Inorganic Phosphorus ◽  
Serum Urea ◽  
Gamma Glutamyltransferase ◽  
Thymol Turbidity

Abstract We studied the effects on 25 analytes of duration of contact of serum with non-anticoagulated blood and of temperature. Serum was separated after blood was allowed to stand, for 0, 2, 4, 6, 8, 24, or 48 h at 4, 23, or 30 degrees C. Results obtained for bilirubin, albumin, zinc sulfate turbidity, thymol turbidity, cholinesterase (EC 3.1.1.8), alkaline phosphatase (EC 3.1.3.1), leucine aminopeptidase (EC 3.4.11.1), amylase (EC 3.2.1.2), total cholesterol, triglycerides, beta-lipoprotein, serum urea nitrogen, creatinine, uric acid, and gamma-glutamyltransferase (EC 2.3.2.2) were not influenced by storage at 4, 24, or 30 degrees C for as long as 48 h. Negligible differences were seen for potassium in sera in contact with cells as long as 24 h at 23 degrees C and for inorganic phosphorus after 48 h at 4 degrees C. However, at 4 degrees C we noted an increase at 8 h, a slight decrease at 30 degrees C. Statistically significant changes were seen for total protein and calcium after 48 h at 30 degrees C; for aspartate aminotransferase (EC 2.6.1.1), and alanine aminotransferase (EC 2.6.1.2), between 8 and 24 h at 23 degrees C and as soon as 6 h at 30 degrees C; for lactate dehydrogenase (EC 1.1.1.27) after 8 h at 30 degrees C and between 8 and 24 h at 23 degrees C; for glucose at 24, 4, or 2 h of storage at 4, 23, or 30 degrees C, respectively; for inorganic phosphorus after 48 h at 23 degrees C or 8 h at 30 degrees C; for potassium after 4 h at 4 degrees C or 24 h at 30 degrees C; and for sodium after 48 h at 4 degrees C or 6 h at 23 or 30 degrees C.

scholarly journals Prognosis of chronic lymphocytic leukemia: a multivariate regression analysis of 325 untreated patients

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 929-936 ◽  
Author(s):  
JS Lee ◽  
DO Dixon ◽  
HM Kantarjian ◽  
MJ Keating ◽  
M Talpaz
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Regression Analysis ◽  
Lactate Dehydrogenase ◽  
Chronic Lymphocytic Leukemia ◽  
Regression Model ◽  
Multivariate Regression ◽  
Risk Groups ◽  
Multivariate Regression Analysis ◽  
Lymphocytic Leukemia

Three hundred twenty-five previously untreated patients with chronic lymphocytic leukemia were analyzed to identify significant prognostic factors for survival. Univariate analysis identified the following characteristics associated with survival: (1) clinical characteristics: age, race, sex, performance status, lymphadenopathy, and hepatosplenomegaly; (2) hematologic parameters: WBC count, absolute lymphocyte and granulocyte counts, hemoglobin level, and platelet count; and (3) biochemical parameters: serum albumin, calcium, uric acid, lactate dehydrogenase, alkaline phosphatase, BUN, and creatinine. Multivariate regression analysis in a randomly selected training subset of 217 patients demonstrated that the combination of uric acid, alkaline phosphatase, lactate dehydrogenase, external lymphadenopathy, and age had the strongest predictive relation to survival time. The resulting model was validated in the remaining independent subset of 108 patients and led to classification of patients into low, intermediate, and high-risk groups with five-year survival rates of 75%, 59%, and 14%, respectively, and with distinctively different annual mortality rates (P less than .01). Both the regression model and Rai staging were highly effective in identifying risk groups among the entire patient population (P less than 0.001). Overall the regression model was superior to Rai staging in defining prognostic risk groups. In addition, it was able to separate patients into significantly different risk categories within each Rai stage, thus improving on the prognostic prediction of individual patients with chronic lymphocytic leukemia.

scholarly journals Метаболічний профіль крові корів, хворих на післяродову гіпокальціємію

2013 ◽  
pp. 76-80
Author(s):  
Л. Л. Юськів ◽  
В. В. Влізло
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Total Protein ◽  
Clinical Signs ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
25 Hydroxycholecalciferol

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.

Quantification of Serum Inorganic Phosphorus, Phosphatase, and Urinary Phosphate without Preliminary Treatment

1970 ◽  
Vol 16 (9) ◽  
pp. 776-780 ◽  
Author(s):  
Jesse F Goodwin
Keyword(s):  
Alkaline Phosphatase ◽  
Ascorbic Acid ◽  
Direct Method ◽  
Reference Method ◽  
Inorganic Phosphorus ◽  
Preliminary Treatment ◽  
Reduction Procedure ◽  
Phosphorus Forms ◽  
A Direct Method ◽  
Serum Phosphatase

Abstract A direct method has been devised for determining serum and urinary inorganic phosphorus and serum phosphatase activity. Inorganic phosphorus forms a phosphomolybdate complex in the presence of borate. The complex is reduced with ascorbic acid. The resulting suspension is solubilized with carbonate and the absorbance measured at 720 nm. Samples containing low to moderate concentrations of bilirubin may be assayed by the method, and it compares favorably with a p-semidine reduction procedure with use of a TCA filtrate. Alkaline phosphatase values obtained by the method in which a β-glycerophosphate substrate is used compare favorably with a reference method in which p-nitrophenolphosphate is used as a substrate. Citrates, fluorides, nitrites, oxalates, and mannitol do not appreciably affect results obtained by the method.

scholarly journals Prognosis of chronic lymphocytic leukemia: a multivariate regression analysis of 325 untreated patients

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 929-936 ◽  
Author(s):  
JS Lee ◽  
DO Dixon ◽  
HM Kantarjian ◽  
MJ Keating ◽  
M Talpaz
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Regression Analysis ◽  
Lactate Dehydrogenase ◽  
Chronic Lymphocytic Leukemia ◽  
Regression Model ◽  
Multivariate Regression ◽  
Risk Groups ◽  
Multivariate Regression Analysis ◽  
Lymphocytic Leukemia

Abstract Three hundred twenty-five previously untreated patients with chronic lymphocytic leukemia were analyzed to identify significant prognostic factors for survival. Univariate analysis identified the following characteristics associated with survival: (1) clinical characteristics: age, race, sex, performance status, lymphadenopathy, and hepatosplenomegaly; (2) hematologic parameters: WBC count, absolute lymphocyte and granulocyte counts, hemoglobin level, and platelet count; and (3) biochemical parameters: serum albumin, calcium, uric acid, lactate dehydrogenase, alkaline phosphatase, BUN, and creatinine. Multivariate regression analysis in a randomly selected training subset of 217 patients demonstrated that the combination of uric acid, alkaline phosphatase, lactate dehydrogenase, external lymphadenopathy, and age had the strongest predictive relation to survival time. The resulting model was validated in the remaining independent subset of 108 patients and led to classification of patients into low, intermediate, and high-risk groups with five-year survival rates of 75%, 59%, and 14%, respectively, and with distinctively different annual mortality rates (P less than .01). Both the regression model and Rai staging were highly effective in identifying risk groups among the entire patient population (P less than 0.001). Overall the regression model was superior to Rai staging in defining prognostic risk groups. In addition, it was able to separate patients into significantly different risk categories within each Rai stage, thus improving on the prognostic prediction of individual patients with chronic lymphocytic leukemia.

Biochemical Profile of Amniotic Fluid to Assess Fetal Maturity

1970 ◽  
Vol 16 (10) ◽  
pp. 843-844 ◽  
Author(s):  
P L Wolf ◽  
Daniel Bloch ◽  
Tashiko Tsu
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Amniotic Fluid ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Uric Acid Concentration ◽  
Biochemical Profile ◽  
Second Trimester ◽  
Fetal Maturity ◽  
Biochemical Analyses

Abstract Twelve different biochemical analyses of amniotic fluid were obtained with the AutoAnalyzer to determine the maturity of the fetus in utero. A group of amniotic fluids from patients early in their second trimester and a second group of amniotic fluids collected at term were compared. Uric acid concentration in amniotic fluid was increased significantly at term, and was the only determination we found valuable in predicting fetal maturity. The values for alkaline phosphatase activity in amniotic fluid were too variable to have predictive usefulness.

scholarly journals Serum proteinogram and biochemistry of Holstein cows in the peripartum period

2019 ◽  
Vol 39 (5) ◽  
pp. 342-347
Author(s):  
Thaís G. Rocha ◽  
Camila Bortoletto ◽  
Daniela G. Silva ◽  
Kalina M.M.G. Simplício ◽  
Luiz F. Zafalon ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Total Protein ◽  
Repeated Measures ◽  
Transition Period ◽  
Serum Levels ◽  
Inorganic Phosphorus ◽  
Holstein Cows ◽  
Total Calcium ◽  
Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

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