scholarly journals Serum proteinogram and biochemistry of Holstein cows in the peripartum period

2019 ◽  
Vol 39 (5) ◽  
pp. 342-347
Author(s):  
Thaís G. Rocha ◽  
Camila Bortoletto ◽  
Daniela G. Silva ◽  
Kalina M.M.G. Simplício ◽  
Luiz F. Zafalon ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Total Protein ◽  
Repeated Measures ◽  
Transition Period ◽  
Serum Levels ◽  
Inorganic Phosphorus ◽  
Holstein Cows ◽  
Total Calcium ◽  
Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

scholarly journals Метаболічний профіль крові корів, хворих на післяродову гіпокальціємію

2013 ◽  
pp. 76-80
Author(s):  
Л. Л. Юськів ◽  
В. В. Влізло
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Total Protein ◽  
Clinical Signs ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
25 Hydroxycholecalciferol

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.

scholarly journals The effect of the Ketostop-EL feed supplement on the clinical and metabolic status of lactating cows

2021 ◽  
Vol 36 ◽  
pp. 06033
Author(s):  
N.B. Nikulina ◽  
E.V. Baidak
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Total Protein ◽  
Chemical Properties ◽  
The Body ◽  
Metabolic Status ◽  
Total Calcium ◽  
Feed Supplement ◽  
Daily Milk Yield ◽  
Daily Milk

A study was conducted on dairy cows aged 3-6 lactation, which were additionally fed with the Ketostop-El supplement at the rate of 300 g per head for 4 weeks after calving. The use of the feed supplement for 14 days led to a decrease in the number of monocytes, total protein, glucose, creatinine, total calcium and the activity of ALT and AST compared to their level before the use of Ketostop-El. Changes in the physical and chemical properties of urine and increase in the average daily milk yield were noted. The use of the supplement for 28 days contributed to a decrease in hematocrit, the sorption capacity of red blood cells, total protein, glucose, bilirubin, creatinine, total calcium, reserve alkalinity, ALT and AST activity and an increase in the number of basophils, eosinophils, and the concentration of inorganic phosphorus compared to the initial data. At the same time, a decrease in the level of protein and ketones in the urine was observed. An increase in the average daily milk yield and the mass fraction of fat in milk was also recorded. The feed supplement "Ketostop-El" has a positive effect on the body of dairy cows, as evidenced by the normalization of the clinical and metabolic status, as well as an increase in the dairy productivity of animals.

scholarly journals Effect of Natural Chinese Herbal Supplements (TCMF4) on Lactation Performance and Serum Biomarkers in Peripartal Dairy Cows

2022 ◽  
Vol 8 ◽  
Author(s):  
Yizhe Cui ◽  
Zhuorui Shan ◽  
Lintong Hou ◽  
Qiuju Wang ◽  
Juan J. Loor ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Total Protein ◽  
Dose Group ◽  
Production Performance ◽  
High Dose Group ◽  
Holstein Cows ◽  
Control Group ◽  
High Dose ◽  
Lactation Performance

This study examined the effect of mixed medicinal herbs from China in the ground form on milk yield and various blood metabolites before and after parturition in Holstein cows. Crushed Agastache rugosus, Scutellaria barbata, Pericarpium citri reticulate, and Radix glycyrrhizae were used to develop TCMF4. Thirty-two Chinese Holstein cows were randomly divided into a control group or groups receiving 0.1, 0.3, or 0.5 kg TCMF4/cow/d from −7 through 21 d relative to parturition. Blood samples for serum isolation were collected at −7, −1, 1, 7, 14, and 21 d relative to parturition and used to measure glucose, β-hydroxybutyric acid (BHBA), total protein, albumin, globulin, and alkaline phosphatase. Milk production was recorded daily for the first 21 d postpartum, and composition was analyzed at 7, 14, and 21 d. Data were analyzed using a one-way analysis of variance (ANOVA) for multiple comparisons. The average milk production during the first 21-d postpartum was 28.7 ± 6.9, 27.2 ± 7.1, 31.2 ± 6.8, and 38.5 ± 6.1 kg/d for control group and groups receiving 0.1, 0.3, or 0.5 kg TCMF4. Thus, average daily milk production increased between 9 to 34% by supplementation with TCMF4 compared with the control group. Compared with the control group, in the middle dose group, milk concentrations of lactose and total protein decreased by 21 and 19%, respectively, at d 7 around parturition, while total solids increased by 23% at d 21 in the high-dose group. Furthermore, compared with the control group, serum BHBA decreased by 50 and 20% at d −1 and 21 around parturition in the high-dose group. Overall, TCMF4 supplementation improved dry matter intake (DMI) and milk production of dairy cows during the periparturient period without adverse effects on liver function, and plasma BHBA concentrations of dairy cows tended to decrease when dietary TCMF4 increased, which suggested that TCMF4 might be used as potential additives in dairy cows to improve production performance.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

scholarly journals Relationship between expression pattern of vitamin D receptor, 1 alpha-hydroxylase enzyme, and chemokine RANTES genes and selected serum parameters during transition period in Holstein dairy cows

2020 ◽  
Vol 7 (1) ◽  
pp. e000339
Author(s):  
Hala A R Saed ◽  
Hussam M M Ibrahim ◽  
Sabry A El-Khodery ◽  
Mohamed A Youssef
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Dairy Cows ◽  
Transition Period ◽  
Post Partum ◽  
Serum Levels ◽  
Vdr Gene ◽  
Genetic Expression ◽  
Enzyme Gene ◽  
Holstein Dairy Cows

ObjectivesThe objective of the present study was to evaluate the pattern of genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1α-OHase) enzyme and chemokine regulated on activation normal T-cell expressed and secreted (RANTES) in peripheral blood of Holstein dairy cows during transition period.MethodsBlood samples were collected from 16 Holstein dairy cows at 3 weeks prior expected date of delivery (EDD), at the day of parturition, and 3 weeks post-partum for assessment of expression profile of studied genes using real-time PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and magnesium (Mg) levels.ResultsCompared with 3 weeks prior EDD, VDR gene expression decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The genetic expression of 1α-OHase enzyme as well as PTH, K, Na and Cl levels increased significantly at the day of parturition. The Ca level decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The P level increased significantly at the day of parturition then decreased significantly at 3 weeks post-partum. Glucose level decreased significantly at the day of parturition and at 3 weeks post-partum. RANTES gene expression showed non-significant changes among the three different time points. The expression of VDR gene had a negative correlation with the expression of 1α-OHase enzyme gene, and serum levels of glucose, PTH, P and K, but had a positive correlation with the serum Ca level. The expression of 1α-OHase enzyme gene had a positive correlation with serum levels of PTH, P and K, but had a negative correlation with the serum Ca level.ConclusionsResults of the current study indicate the importance of monitoring the genetic expression of VDR and 1α-OHase enzyme as indicators of metabolic changes during transition period, suggesting that they are candidate genes to judge the health status of dairy cows during such period.

scholarly journals The effect of Glycoline® on reproductive efficiency in high-producing dairy cows

2016 ◽  
pp. 5163-5176
Author(s):  
Marcelo R. Del Campo ◽  
Marco Gonzalez T ◽  
Hans Andresen ◽  
Freddy A. Cea
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Transition Period ◽  
Holstein Cows ◽  
Reproductive Efficiency ◽  
Control Group ◽  
Ovarian Activity ◽  
Uterine Infection ◽  
Uterine Involution ◽  
Downer Cow Syndrome

ABSTRACT Objective. Determine the effect of Glycoline® on reproductive efficiency in high producing dairy cows. Materials and methods. 100 Holstein cows one month before delivery were selected. About 21 days before parturition they were put in a barn and were randomized in two groups: Glycoline® Group (GG, n=50), 300 g/day of Glycoline® for 21 days antepartum and 250 g/day of GlycoLine® over the following 21 days postpartum, and the Control Group (CG, n=50) with the same feed and silage ration as GG during the same period, but without the addition of Glycoline®. Events and reproductive variables of the cows were recorded for 202 days. The data were systematized, analyzed and statistically compared. Results. Comparisons were made between GG and CG respectively: Retained placenta (0.0 vs. 12.0%; p=0.027), downer cow syndrome (14.3 vs. 44.0%; p=0.002), uterine involution (64.6 vs. 36.4%; p=0.019 ), uterine infection (10.4% vs. 35.5%; p=0.006), no ovarian activity (6.3 vs. 25.6%; p=0.018), follicular cysts (0.0 vs. 18.2%, p = 0.002), luteal structures (25.0 vs. 9.3% ; p=0.058), mean estrus presentation (40.1% vs. 63.5%; p=0.033) inseminated females (79.6 vs. 68.0%; p=0.017), pregnancy rate at day 150 (57.1 vs. 46.0%; p≥0.317 ) and intervals (days): calving to 1st estrus (39.8 vs. 63.2; p≤0.006), calving to IA (62.4 vs. 87.5; p≤0.006) calving to conception (81.7 vs. 93.6; p≤0.006; p=0.103). Conclusions. Results suggest that dietary Glycoline® added in the transition period improves reproductive efficiency of high-producing dairy cows. RESUMEN Objetivos. Determinar el efecto del Glycoline® sobre la eficiencia reproductiva en vacas lecheras de alta producción. Materiales y Métodos. Se seleccionaron 100 vacas Holstein un mes antes del parto. Alrededor de 21 días antes del parto se ubicaron en un establo y se asignaron al azar a dos Grupos: Grupo Glycoline® (GG; n=50), 300 g/día de Glycoline® durante 21 días preparto y 250 g/día de Glycoline® durante los siguientes 21 días posparto y Grupo Control (GC, n=50) la misma ración de concentrado y ensilaje que el GG durante el mismo período de tiempo, pero sin la adición de Glycoline®. Se registraron los eventos y las variables reproductivas de las vacas durante 202 días. Los datos fueron sistematizados, analizados y comparados estadísticamente. Resultados. Comparaciones entre el GG y GC respectivamente: Retención de placenta (0,0 vs 12.0%; p=0.027), síndrome de vaca caída (14.3 vs 44.0%; p=0.002), involución uterina (64.6 vs 36.4%; p=0.019), infección uterina (10.4% vs 35.5%; p=0.006), sin actividad ovárica (6.3 vs 25.6%; p=0.018), quistes foliculares (0.0 vs 18.2%, p=0.002), estructuras luteales (25.0 vs 9.3%; p=0.058), promedio presentación de celo (40.1% vs 63.5%; p=0.033), hembras inseminadas (79.6 vs 68.0%; p=0.017), tasa de preñez al día 150 (57.1 vs 46.0%; p≥0.317) y intervalos de tiempo (días): parto 1er -celo (39.8 vs 63.2; p≤0.006), parto-IA (62.4 vs 87.5; p≤0.006) y parto-preñez (81.7 vs 93.6; p≤0.006; p=0.103). Conclusiones. Los resultados sugieren que el Glycoline® adicionado en la dieta en el período de transición, mejora la eficiencia reproductiva de vacas de alta producción.

scholarly journals EFFECT OF FEED ADDITIVE ON MINERAL HOMEOSTASIS OF COWS WITH KETOSIS

2020 ◽  
Vol 21 (1) ◽  
pp. 247-251
Author(s):  
O. Shkilnyk ◽  
I. Petrukh ◽  
D. Ostapiv ◽  
M. Kozak ◽  
V. Vlizlo ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Hormonal Regulation ◽  
Ketone Bodies ◽  
Mineral Metabolism ◽  
Inorganic Phosphorus ◽  
Feed Additives ◽  
Feed Additive ◽  
Total Calcium ◽  
Before And After

The article describes the study of mineral metabolism in patients with cow ketosis and during feeding of our developed feed additive, which includes crushed hop cones, vitamin E and protected from cleavage in the rumen amino acids ˗ methionine, choline and carnitine. In the blood of cows with ketosis, before and after the use of feed additives studied the content of total calcium and inorganic phosphorus, alkaline phosphatase activity, determined the ratio of alkaline phosphatase and total calcium, the concentration of thyroid hormones (parathyroid hormone) and thyroid, thyroid also the amount of ketone bodies in the urine and β-hydroxybutyrate in the blood. It was found that twenty days of feeding cows with ketosis, feed additives leads to a decrease in ketonuria and ketonemia, as well as the normalization of mineral metabolism. At the end of the experiment, the content of total calcium and inorganic phosphorus in the blood of cows increased, the activity of alkaline phosphatase and the ratio of ALP / Ca decreased. The normalization of mineral metabolism in cows was also evidenced by its balanced hormonal regulation. The concentration of parathyroid hormone decreased in the blood plasma and calcitonin increased. According to the results of research, it is established that our proposed feed additive contributes to the normalization of mineral metabolism, and can also be used for prevention and in the complex treatment of cows with ketosis.

scholarly journals THE INFLUENCE OF NEW BEDDING MATERIAL ON MORPHOBIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS OF BLOOD OF GOSLINGS OF BROILERS

2021 ◽  
Vol 247 (3) ◽  
pp. 244-248
Author(s):  
V.G. Sofronov ◽  
◽  
R.N. Fayzrakhmanov ◽  
N.I. Danilova ◽  
E.L. Kuznetsova ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Allergic Reactions ◽  
Bedding Material ◽  
Total Calcium ◽  
Immunological Parameters ◽  
Mucous Membranes

The harmlessness of the new hygienic bedding material and the effect of broilers on the body have been studied. During the experiment, it was found that the new hygienic litter material, representing waste from the woodworking industry, treated with thermochemical and biotechnological methods, does not have an irritating effect on the skin, does not irritate the mucous membranes of the eyes and allergic reactions in rabbits, but, on the contrary, improves certain morphobiochemical and immunological parameters of the body’s blood are goslings. Ultimately, this was reflected in an increase in red blood cells by 7.04 %, hemoglobin – 5.20 %, total protein – 6.3 %, total calcium – 5.8 %, inorganic phosphorus – 5.1 %, glucose – 5,4 % compared with the control.

scholarly journals Effect of using trace minerals (copper, zinc, selenium, and manganese) and vitamins A and E on the metabolic profile of Holstein cows in the transition period

2019 ◽  
Vol 40 (5) ◽  
pp. 1879
Author(s):  
Silvana Giacomini Collet ◽  
Rejane Dos Santos Sousa ◽  
Enrico Lippi Ortolani ◽  
André Thaler Neto ◽  
Michèli Cristina Carpeggiani ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Transition Period ◽  
Block Design ◽  
Trace Minerals ◽  
Holstein Cows ◽  
Control Group ◽  
Significance Level ◽  
Significant Difference ◽  
Copper Zinc ◽  
Metabolic Challenge

The aim of this study was to assess the metaphylactic effect of subcutaneous doses of trace minerals (copper, zinc, selenium, and manganese) and vitamins A and E on biochemical (NEFA, BHBA, fructosamine, urea, triglycerides, HDL, and calcium) and hormonal parameters (cortisol, insulin, and IGF-1) of Holstein cows in the transition period. Sixty animals kept in a semi-confinement system, with an average production of 22 liters of milk per day (Farm 1) and 24 liters of milk per day (Farm 2) and a diet according to NRC (2001) with a low metabolic challenge, were divided into two groups: a treatment group, supplemented with trace minerals and vitamins (MTVG) (n=30), and a control group (CG) (n=30). The collection of blood samples was performed at the moments M1 (21 days prepartum), M2 (14 days prepartum), M3 (7 days prepartum), M4 (parturition day), M5 (7 days postpartum), M6 (14 days postpartum), and M7 (21 days postpartum). The experimental design was a randomized block design and the data were submitted to analysis of variance with repeated measures in time within the random variable cow. Differences were considered significant at 5% significance level and trend at 10% level. No difference was observed between treatment and the interaction treatment × day in the assessed parameters. However, some variables showed a significant difference of day. NEFA levels (P < 0.0001) were higher on the parturition day. BHBA concentrations were higher after parturition (P=0.0031), with a peak at the third week of lactation. Fructosamine showed increasing values until the parturition day (P=0.0373), in which higher concentrations were observed. Average values of HDL were higher at 21 days postpartum (P < 0.0001). Triglycerides levels remained high during the prepartum period but showed a sudden drop on the parturition day (P < 0.0001), with lower values postpartum. Regarding serum calcium and IGF-1, lower values were observed on the parturition day (P < 0.001) for both parameters. Cortisol presented a difference regarding day, with higher values on the parturition day (P=0.0151). Thus, the use of trace minerals and vitamins A and E in animals with an adequate body score and low metabolic challenge presents a low response to biochemical and hormonal parameters.

Effect of feeding diet containing fish oil and canola oil from transition period on milk production and components of Holstein cows in early lactation

2009 ◽  
Vol 2009 ◽  
pp. 157-157
Author(s):  
T Vafa ◽  
A Naserian ◽  
A Heravi Moussavi ◽  
R Valizadeh ◽  
M Danesh Mesgaran
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Dairy Cows ◽  
Milk Production ◽  
Canola Oil ◽  
Unsaturated Fatty Acids ◽  
Transition Period ◽  
Holstein Cows ◽  
Early Lactation ◽  
Effect Of Feeding

There are too many physiological and nutritional factors which can influence lactation performance of early lactation Holstein dairy cows. Supplemental fat sources are utilized in rations for dairy cows as a common method to increase the energy density of the diet, especially in early lactation (Juchem et al., 2007). The fat sources also have a positive effect on milk fatty acid profile. Canola seed which contains 40% fat is an excellent source of dietary fat high in unsaturated fatty acids and protein for dairy animals. Canola oil contains 51% oleic, 25% linoleic, and 14% linolenic acids. Fish oil contains relatively high concentrations fatty acids of the n-3 family. It seems that feeding a blend of different oils will have more positive productive response than individual usage of them. The aim of this study was to evaluate the effect of feeding diets containing fish oil and canola oil starting from transition period on milk production and composition of early lactating Holstein cows.

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