Ultramicroscale Determination of Clinical Chemical Values for Blood during the First Four Days of Postnatal Life

1975 ◽  
Vol 21 (6) ◽  
pp. 746-750 ◽  
Author(s):  
Larissa deBaare ◽  
Jean Lewis ◽  
Helen Sing
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Total Protein ◽  
Inorganic Phosphorus ◽  
Postnatal Life ◽  
Total Proteins ◽  
Blood Constituents ◽  
Clinical Chemical ◽  
Term Newborns

Abstract Ultramicro procedures requiring 5-10 µl of serum or blood per analysis were used in determining blood constituents of healthy full-term newborns during the first four days of life. The resulting values appeared to be influenced by age, sex, and race. Values for total protein, albumin, urea nitrogen, and uric acid in serum decreased with time; serum inorganic phosphorus and whole-blood aldosaccharoses increased. Serum from females had higher values than that from males for total proteins, albumin, and inorganic phosphorus. The values for serum calcium and alkaline phosphatase were consistently higher in Negro than in white infants; values for uric acid were higher in the latter.

Chemical and clinical evaluation of the random access analyzer "RA-1000".

1984 ◽  
Vol 30 (3) ◽  
pp. 364-368 ◽  
Author(s):  
M K Schwartz ◽  
B E Statland ◽  
J Coughlin ◽  
C Eisen ◽  
M Fleisher ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Random Access ◽  
Inorganic Phosphorus ◽  
Production Model ◽  
Excellent Correlation ◽  
Gamma Glutamyltransferase

Abstract In the RA-1000, a random-access discrete analyzer, an inert fluorocarbon fluid is used to prevent interaction and carryover. Production-model instruments were evaluated in two laboratories with respect to determination of glucose, creatinine, total protein, inorganic phosphorus, cholesterol, alkaline phosphatase, lactate dehydrogenase, creatine kinase, gamma-glutamyltransferase, and aspartate and alanine aminotransferases. Within-run, among-run, and day-to-day (for 15 days) precision was assessed, and results were correlated with those obtained by the methods routinely in use in our departments. Precision was excellent, correlation acceptable.

Serum-constituents analyses: effect of duration and temperature of storage of clotted blood.

1981 ◽  
Vol 27 (1) ◽  
pp. 35-38 ◽  
Author(s):  
T Ono ◽  
K Kitaguchi ◽  
M Takehara ◽  
M Shiiba ◽  
K Hayami
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Zinc Sulfate ◽  
Leucine Aminopeptidase ◽  
Inorganic Phosphorus ◽  
Serum Urea ◽  
Gamma Glutamyltransferase ◽  
Thymol Turbidity

Abstract We studied the effects on 25 analytes of duration of contact of serum with non-anticoagulated blood and of temperature. Serum was separated after blood was allowed to stand, for 0, 2, 4, 6, 8, 24, or 48 h at 4, 23, or 30 degrees C. Results obtained for bilirubin, albumin, zinc sulfate turbidity, thymol turbidity, cholinesterase (EC 3.1.1.8), alkaline phosphatase (EC 3.1.3.1), leucine aminopeptidase (EC 3.4.11.1), amylase (EC 3.2.1.2), total cholesterol, triglycerides, beta-lipoprotein, serum urea nitrogen, creatinine, uric acid, and gamma-glutamyltransferase (EC 2.3.2.2) were not influenced by storage at 4, 24, or 30 degrees C for as long as 48 h. Negligible differences were seen for potassium in sera in contact with cells as long as 24 h at 23 degrees C and for inorganic phosphorus after 48 h at 4 degrees C. However, at 4 degrees C we noted an increase at 8 h, a slight decrease at 30 degrees C. Statistically significant changes were seen for total protein and calcium after 48 h at 30 degrees C; for aspartate aminotransferase (EC 2.6.1.1), and alanine aminotransferase (EC 2.6.1.2), between 8 and 24 h at 23 degrees C and as soon as 6 h at 30 degrees C; for lactate dehydrogenase (EC 1.1.1.27) after 8 h at 30 degrees C and between 8 and 24 h at 23 degrees C; for glucose at 24, 4, or 2 h of storage at 4, 23, or 30 degrees C, respectively; for inorganic phosphorus after 48 h at 23 degrees C or 8 h at 30 degrees C; for potassium after 4 h at 4 degrees C or 24 h at 30 degrees C; and for sodium after 48 h at 4 degrees C or 6 h at 23 or 30 degrees C.

scholarly journals Метаболічний профіль крові корів, хворих на післяродову гіпокальціємію

2013 ◽  
pp. 76-80
Author(s):  
Л. Л. Юськів ◽  
В. В. Влізло
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Total Protein ◽  
Clinical Signs ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
25 Hydroxycholecalciferol

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.

Effects of some anti-inflammatory drugs on 12 blood constituents: protocol for the study of in vivo effects of drugs.

1985 ◽  
Vol 31 (7) ◽  
pp. 1141-1143 ◽  
Author(s):  
Z Jelić-Ivanović ◽  
S Spasić ◽  
N Majkić-Singh ◽  
P Todorović
Keyword(s):  
Uric Acid ◽  
Acetylsalicylic Acid ◽  
Total Protein ◽  
Inorganic Phosphate ◽  
Drug Effects ◽  
Urea Nitrogen ◽  
Blood Constituents ◽  
Inflammatory Drugs ◽  
In Vivo Effects

Abstract We investigated the in vivo effects of acetylsalicylic acid, diclofenac, indomethacin, ibuprofen, and ketoprofen on the concentrations of various blood constituents. Total protein, glucose, calcium, and inorganic phosphate were not significantly affected by any of these drugs. Ketoprofen had no definite influence on any constituent. Acetylsalicylic acid induced an increase in cholesterol, triglyceride, and iron; albumin, uric acid, and creatinine decreased with ibuprofen therapy. Urea nitrogen increased in patients treated with diclofenac or indomethacin. Our protocol for the study of in vivo drug effects is discussed.

scholarly journals Serum proteinogram and biochemistry of Holstein cows in the peripartum period

2019 ◽  
Vol 39 (5) ◽  
pp. 342-347
Author(s):  
Thaís G. Rocha ◽  
Camila Bortoletto ◽  
Daniela G. Silva ◽  
Kalina M.M.G. Simplício ◽  
Luiz F. Zafalon ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Total Protein ◽  
Repeated Measures ◽  
Transition Period ◽  
Serum Levels ◽  
Inorganic Phosphorus ◽  
Holstein Cows ◽  
Total Calcium ◽  
Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

scholarly journals Serum biochemistry of 4-day-old ostriches (Struthio camelus)

2008 ◽  
Vol 28 (9) ◽  
pp. 423-426 ◽  
Author(s):  
Renata L. Miranda ◽  
Antonio V. Mundim ◽  
Álisson S. Costa ◽  
Ruyther V. Guimarães ◽  
Frederico O.C. Silva
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Biochemistry ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Total Proteins ◽  
Males And Females

An analysis was made of 30 four-day-old ostriches to evaluate their protein, metabolite, mineral, and serum enzyme profiles, to correlate them with the birds' sex. The values obtained were: Total proteins 3.59±0.72g/dL, albumin 1.04±0.14g/dL, globulins 2.51±0.56g/dL, A:G ratio 0.43± 0.07, total cholesterol 615.10±101.15mg/dL, high density lipoprotein cholesterol (HDL-C) 132.72±20.33mg/dL, low density lipoprotein cholesterol (LDL-C) 454.93±90.81mg/dL, very low density lipoprotein cholesterol (VLDL-C) 27.45±9.96mg/dL, triglycerides 137.23±49.78mg/dL, uric acid 6.24±2.15mg/dL, urea 18.27±12.33mg/dL, creatinine 0.30±0.04mg/dL, total calcium 9.38± 0.76mg/dL, ionized calcium 7.17±0.64mg/dL, phosphorus 6.96±0.91mg/dL, Ca:P ratio 1.37±0.21, iron 24.74±13.02µg/dL, sodium 142.03±6.17mEq/L, chlorides 109.59± 4.99mEq/L, aspartate aminotransferase (AST) 200.67±31.42 U/L, alanine aminotransferase (ALT) 3.90±1.92 U/L, γ-glutamyltransferase (GGT) 1.18±0.73 U/L, alkaline phosphatase (ALP) 597.30± 231.36 U/L, and creatine kinase (CK) 2348.30±755.60 U/L. Males and females showed significant differences in total proteins, globulins, alkaline phosphatase, A:G ratio, and uric acid.

scholarly journals Multianalyte Serum Analysis Using Mid-Infrared Spectroscopy

1998 ◽  
Vol 35 (5) ◽  
pp. 624-632 ◽  
Author(s):  
R Anthony Shaw ◽  
Steven Kotowich ◽  
Michael Leroux ◽  
Henry H Mantsch
Keyword(s):  
Uric Acid ◽  
Total Protein ◽  
Correlation Coefficients ◽  
Standard Errors ◽  
Infrared Transmission ◽  
Serum Samples ◽  
Chemical Methods ◽  
Mid Infrared ◽  
Low Concentrations ◽  
Clinical Chemical

This study assesses the potential for using mid-infrared (mid-IR) spectroscopy of dried serum films as the basis for the simultaneous quantitation of eight serum analytes: Total protein, albumin, triglycerides, cholesterol, glucose, urea, creatinine and uric acid. Infrared transmission spectra were acquired for 300 serum samples, each analysed independently using accepted reference clinical chemical methods. Quantitation methods were based upon the infrared spectra and reference analyses for 200 specimens, and the models validated using the remaining 100 samples. Standard errors in the IR-predicted analyte levels (Sy/x) were 2.8 g/L (total protein), 2.2 g/L (albumin), 0.23 mmol/L (triglycerides), 0.28 mmol/L (cholesterol), 0.41 mmol/L (glucose) and 1.1 mmol/L for urea, with correlation coefficients (IR vs reference analyses) of 0.95 or better. The IR method emerged to be less suited for creatinine (S y/ x = μmol/L) and uric acid (S y/x = 140 μmol/L) due to the relatively low concentrations typical of these analytes.

THE STATE OF HEPATOBILIARY SYSTEM IN TERM NEWBORNS FROM MOTHERS WHO SUFFERED INFLUENZA VIRUS A(H3N2) IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (62) ◽  
pp. 72-75
Author(s):  
Наталия Ишутина ◽  
Nataliya Ishutina ◽  
Игорь Гориков ◽  
Igor Gorikov ◽  
Ирина Андриевская ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Total Cholesterol ◽  
Total Protein ◽  
Influenza A ◽  
Influenza Infection ◽  
Direct Bilirubin ◽  
Control Group ◽  
Umbilical Blood ◽  
Antibody Titers ◽  
Term Newborns

The functional state of liver in 112 term newborns, who depending on their antenatal ontogenesis were divided into 4 groups, was studied. The first group (control) was made of 30 healthy newborns from mothers with physiological course of pregnancy; the second group consisted of 30 children whose mothers suffered influenza A(H3N2) (antibody titers were 1:4-1:16), the third group had 27 newborns with antenatal anamnesis complicated with influenza A(H3N2) (antibody titers were 1:8-1:32) and the fourth group had 25 children whose mothers suffered A(H3N2) (antibody titers were 1:32-1:128) at early stages of gestation. In newborns in the serum of the umbilical blood there were found the contents of total protein, albumins, total, indirect and direct bilirubin, activity of alanine aminotransferase, serum glutamate-oxaloacetate-transaminase, gamma-glutamyl transpeptidase, lactic dehydrogenase and alkaline phosphatase, concentration of total cholesterol, lipoproteins of low density and triglycerides. In babies from mothers who suffered influenza infection with antibody titers 1:32-1:128 there was the decrease of total protein till 55.1±1.27 g/l (in the control group it was 62.1±1.45 g/l; р&#60;0.01) and albumins till 34.2±1.01 g/l (in the control it was 38.2±1.13 g/l; р&#60;0.05) against the increase of total bilirubin till 35.5±1.29 mcmole/l (in the control it was 25.3±1.67 g/l; р&#60;0.001), of indirect bilirubin till 33.2±1.28 mcmole/l (in the control it was 23.4±1.67 mcmole/l; р&#60;0.001), of direct bilirubin till 2.33±0.10 mcmole/l (in the control it was 1.91±0.09; р&#60;0.01) and the activity of alkaline phosphatase till 175.7±10.62 МЕ/l (in the control it was 141.6±6.39 МЕ/l; р&#60;0.01). This showed the suppression of protein-synthesizing and pigment function of the liver as well as of the process of cholestasis. At the same time there was the decrease of total cholesterol till 1.83±0.06 mmole/l (in the control it was 2.08±0.07 mmole/l; р&#60;0.01) and lipoproteins of high density till 1.02±0.06 mmole/l (in the control it was 1.27±0.06 mmole/l; р&#60;0.01). Their concentration causes the stability of cellular membranes and hypothalamic-pituitarium-adrenal system in newborns to unfavourable factors in the early postnatal period of their development.

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