Levels of Calcium, Corrected Calcium, Alkaline Phosphatase and Inorganic Phosphorus in Patients’ Serum with β-Thalassemia Major on Subcutaneous Deferoxamine

2014 ◽  
Vol 02 (02) ◽  
Author(s):  
Muhammad Saboor
Keyword(s):  
Alkaline Phosphatase ◽  
Thalassemia Major ◽  
Inorganic Phosphorus

scholarly journals Метаболічний профіль крові корів, хворих на післяродову гіпокальціємію

2013 ◽  
pp. 76-80
Author(s):  
Л. Л. Юськів ◽  
В. В. Влізло
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Total Protein ◽  
Clinical Signs ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
25 Hydroxycholecalciferol

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.

Quantification of Serum Inorganic Phosphorus, Phosphatase, and Urinary Phosphate without Preliminary Treatment

1970 ◽  
Vol 16 (9) ◽  
pp. 776-780 ◽  
Author(s):  
Jesse F Goodwin
Keyword(s):  
Alkaline Phosphatase ◽  
Ascorbic Acid ◽  
Direct Method ◽  
Reference Method ◽  
Inorganic Phosphorus ◽  
Preliminary Treatment ◽  
Reduction Procedure ◽  
Phosphorus Forms ◽  
A Direct Method ◽  
Serum Phosphatase

Abstract A direct method has been devised for determining serum and urinary inorganic phosphorus and serum phosphatase activity. Inorganic phosphorus forms a phosphomolybdate complex in the presence of borate. The complex is reduced with ascorbic acid. The resulting suspension is solubilized with carbonate and the absorbance measured at 720 nm. Samples containing low to moderate concentrations of bilirubin may be assayed by the method, and it compares favorably with a p-semidine reduction procedure with use of a TCA filtrate. Alkaline phosphatase values obtained by the method in which a β-glycerophosphate substrate is used compare favorably with a reference method in which p-nitrophenolphosphate is used as a substrate. Citrates, fluorides, nitrites, oxalates, and mannitol do not appreciably affect results obtained by the method.

scholarly journals Serum proteinogram and biochemistry of Holstein cows in the peripartum period

2019 ◽  
Vol 39 (5) ◽  
pp. 342-347
Author(s):  
Thaís G. Rocha ◽  
Camila Bortoletto ◽  
Daniela G. Silva ◽  
Kalina M.M.G. Simplício ◽  
Luiz F. Zafalon ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Total Protein ◽  
Repeated Measures ◽  
Transition Period ◽  
Serum Levels ◽  
Inorganic Phosphorus ◽  
Holstein Cows ◽  
Total Calcium ◽  
Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION I. THE KINETICS OF PHOSPHATASE ACTION ON VARIOUS SUBSTRATES

1953 ◽  
Vol 31 (1) ◽  
pp. 1-7
Author(s):  
Neil B. Madsen ◽  
Jules Tuba
Keyword(s):  
Alkaline Phosphatase ◽  
Average Energy ◽  
Inorganic Phosphorus ◽  
Intestinal Alkaline Phosphatase ◽  
Egg Lecithin ◽  
Michaelis Constants ◽  
Inhibition Studies ◽  
Kinetics Of ◽  
Hydrolysis Of

The kinetics of intestinal alkaline phosphatase action on sodium β-glycerophosphate, glucose 6-phosphate, and egg lecithin have been studied and compared. The Michaelis constants indicate that the enzyme shows considerably less affinity for lecithin than for the other two substrates, and the approximate ratio of activity with lecithin, glucose 6-phosphate, and sodium β-glycerophosphate is 11 : 78.5 : 100. The energies of activation for the hydrolysis of the three substrates do not differ appreciably and the average energy of activation is 14,500 calories per gram-mole. The similarity of the energies of activation together with results from inhibition studies indicate that in all probability the same enzyme is responsible for the release of inorganic phosphorus from each of the three substrates.

Prevalence of Hypoparathyroidism in Children and Young Adults with Thalassemia Major.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3850-3850
Author(s):  
Satya Prakash Yadav ◽  
Anupam Sachdeva ◽  
Subash C. Arya ◽  
Virender K. Khanna ◽  
Archana D. Arya
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Calcium ◽  
Age Of Onset ◽  
Ferritin Level ◽  
Thalassemia Major ◽  
Tolerance Test ◽  
Endocrine Disorders ◽  
Female Ratio ◽  
Excess Iron ◽  
Normal Mean

Abstract Endocrinopathies are frequently reported in children with thalassemia major. Affected children at our center are transfused regularly to maintain hemoglobin of at least 8–9 gm/dl. Most subjects are chelated with Desferrioxamine or Deferiprone or a combination of the two drugs, although a majority of children have poor compliance and are poorly chelated. Hypoparathyroidism usually occurs in thalassemia major due to excess iron deposition in the parathyroid glands. All patients suffering from thalassemia major are screened routinely at our clinic for their bone metabolism. The fasting serum calcium, phosphorus, intact parathormone and alkaline phosphatase levels are checked annually to screen for hypoparathyroidism and Vitamin D deficiency. A diagnosis of hypoparathyroidism is made if the serum calcium is low, phosphorus is high, alkaline phosphatase level is normal and serum parathormone level (intact) is low normal. Mean S. ferritin levels of all the subjects were calculated by averaging the ferritin values over the last 5 years. We analyzed the screening results for hypoparathyroidism in 110 subjects with Thalassemia Major between the ages of 10–25 years. Of these 110 subjects 19 (17.3%) were found to be suffering from hypoparathyroidism and 91 patients had a normal screen. Mean S. ferritin level was 5997ng/ml in the affected subjects (ranging from 2900 to 10,904 ng/ml), compared with the mean S. ferritin level of 3793ng/ml in the unaffected subjects, which was clinically significant, showing a direct correlation between the poor chelation and development of hypoparathyroidism. Only 2 subjects from our affected group had an average ferritin of 2000–3000 ng/ml, although they also had other endocrine disorders such as short stature and hypothyroidism. Of the affected subjects, 9 were males and 10 were females (M: F = 1.1:1). The reported male: female ratio for hypoparathyroidism in thalassemics is 3:1. Age of onset of hypoparathyroidism ranged from 10.9 years to 23 years with mean age of onset being 16 years. The youngest subject (10.9 years) had an average ferritin of 2908 ng/ml. All the affected subjects above 15 years of age had hypogonadism, 7 of the 19 affected subjects (36.84%) had diabetes, 4 (21%) of them had an abnormal glucose tolerance test, and 5 (26.3%) had hypothyroidism. The incidence of hypoparathyroidism reported in our group of patients is higher compared to those reported from other continents (3.6–4.5%) probably due to poor chelation., both males and females are equally affected and the age of onset is earlier compared to reports from other countries.. This suggests that high ferritin levels due to poor chelation is a major factor in the development of hypoparathyroidism and most subjects who develop it, have multiple endocrine disorders.

scholarly journals D-vitamin status of cattle depending on the conditions of detention and physiological state

2018 ◽  
Vol 20 (88) ◽  
pp. 141-146
Author(s):  
L. L. Yuskiv ◽  
V. Vlizlo
Keyword(s):  
Alkaline Phosphatase ◽  
Physiological State ◽  
Inorganic Phosphorus ◽  
Postnatal Period ◽  
Winter Period ◽  
Dry Period ◽  
Black And White ◽  
Vitamin Status ◽  
Calcium Phosphorus ◽  
Dairy Breed

The article contains data on the content of active metabolite of vitamin D3 – 25OHD3, calcium, phosphorus, magnesium and activity of alkaline phosphatase in the blood of cows and their calves in the early postnatal period. The experiment was conducted on cows-analogues of the Ukrainian Black-and-White dairy breed during the dry period and after calving and on calves received from them. The experiment was performed during the winter period of the stall at the Lviv region farm. The blood for tests was collected from the jugular vein before morning feeding at 3–5 days before calving and in the 5th–7th and 55th–60th days after calving. The blood from the calves was collected in the following dates: on the first day and at 5th–7th days old and at 55–60th days old.  It was established that, the content of 25-hydroxycolecalciferol in blood calves on the first day after birth was 18.90 ± 2.08 nmol/l. In subsequent periods of study, its level decreased, in particular, at 5th−7th days old it was 1.52 times lower (P < 0.05), and in the at 55–60th days old – 2.07 times (P < 0.01) than on the first day old age during the winter period of the stall. Changes in the content of 25OHD3, total calcium and its fractions, inorganic phosphorus, magnesium and activity of alkaline phosphatase and its isoenzymes in the blood of cows in the prenatal and lactation periods have been determined. In particular, the content of 25OHD3 was 18.7 ± 2.27 nmol/l at 3–5 days before calving in the blood of cows, on the 5–7th day after calving continued to decrease and amounted to 15.8 ± 0.83 nmol/l, and on the 55–60th day of the postpartum period it increased to 20.5 ± 2.08 nmol/l.

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