scholarly journals Метаболічний профіль крові корів, хворих на післяродову гіпокальціємію

2013 ◽  
pp. 76-80
Author(s):  
Л. Л. Юськів ◽  
В. В. Влізло
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Total Protein ◽  
Clinical Signs ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
25 Hydroxycholecalciferol

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.

scholarly journals Serum proteinogram and biochemistry of Holstein cows in the peripartum period

2019 ◽  
Vol 39 (5) ◽  
pp. 342-347
Author(s):  
Thaís G. Rocha ◽  
Camila Bortoletto ◽  
Daniela G. Silva ◽  
Kalina M.M.G. Simplício ◽  
Luiz F. Zafalon ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Total Protein ◽  
Repeated Measures ◽  
Transition Period ◽  
Serum Levels ◽  
Inorganic Phosphorus ◽  
Holstein Cows ◽  
Total Calcium ◽  
Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

scholarly journals EFFECT OF FEED ADDITIVE ON MINERAL HOMEOSTASIS OF COWS WITH KETOSIS

2020 ◽  
Vol 21 (1) ◽  
pp. 247-251
Author(s):  
O. Shkilnyk ◽  
I. Petrukh ◽  
D. Ostapiv ◽  
M. Kozak ◽  
V. Vlizlo ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Hormonal Regulation ◽  
Ketone Bodies ◽  
Mineral Metabolism ◽  
Inorganic Phosphorus ◽  
Feed Additives ◽  
Feed Additive ◽  
Total Calcium ◽  
Before And After

The article describes the study of mineral metabolism in patients with cow ketosis and during feeding of our developed feed additive, which includes crushed hop cones, vitamin E and protected from cleavage in the rumen amino acids ˗ methionine, choline and carnitine. In the blood of cows with ketosis, before and after the use of feed additives studied the content of total calcium and inorganic phosphorus, alkaline phosphatase activity, determined the ratio of alkaline phosphatase and total calcium, the concentration of thyroid hormones (parathyroid hormone) and thyroid, thyroid also the amount of ketone bodies in the urine and β-hydroxybutyrate in the blood. It was found that twenty days of feeding cows with ketosis, feed additives leads to a decrease in ketonuria and ketonemia, as well as the normalization of mineral metabolism. At the end of the experiment, the content of total calcium and inorganic phosphorus in the blood of cows increased, the activity of alkaline phosphatase and the ratio of ALP / Ca decreased. The normalization of mineral metabolism in cows was also evidenced by its balanced hormonal regulation. The concentration of parathyroid hormone decreased in the blood plasma and calcitonin increased. According to the results of research, it is established that our proposed feed additive contributes to the normalization of mineral metabolism, and can also be used for prevention and in the complex treatment of cows with ketosis.

scholarly journals HYPOCALCEMIA AFTER PARATHYROIDECTOMY

2020 ◽  
Vol 23 (2) ◽  
pp. 75-79
Author(s):  
L. P. Kotelnikova ◽  
G. Yu. Mokina ◽  
N. G. Polyakova
Keyword(s):  
Parathyroid Hormone ◽  
Calcium Level ◽  
Clinical Symptoms ◽  
Clinical Signs ◽  
Total Serum ◽  
Primary Group ◽  
Total Calcium ◽  
Baseline Serum ◽  
Median Baseline ◽  
Total Serum Calcium

The aim of the study was to estimate the frequency and timing of hypocalcemia after surgical treatment for primary, secondary and tertiary hyperparathyroidism. Materials and methods. 21 patients were operated for hyperparathyroidism, 15 - for primary (group 1), 6 - for secondary and tertiary (group 2). In I group the median baseline level of total serum calcium was 3.06 mmol/l, phosphorus0.9 mmol/l, and parathyroid hormone360 pmol/l. In II group all patients were on program dialysis for end-stage chronic kidney failure for at least five years. The median baseline serum total calcium level was 2.29 mmol/l, phosphorus2.64 mmol/l, and parathyroid hormone-1822 pmol/l. Results. A day after removal of the parathyroid adenoma (1 group) the level of calcium and phosphorus was normalized, the content of parathyroid hormone (median 21.4 pmol/l) significantly decreased. In one case (6.7%) on the fifth day there were clinical signs of hypocalcemia and the level of calcium decreased to 1.86 mmol/l. All patients of the second group underwent subtotal parathyroidectomy. After a day the level of parathyroid hormone significantly decreased (median227 pmol/l). The phosphorus content has returned to normal. The calcium level in all cases exceeded 2 mmol/l. On day 4-5 the total calcium content decreased and ranged from 1.14 mmol/l to 2.04 mmol/l. Four patients (66,7%) showed clinical signs of hypocalcemia. It was found that the development of hypocalcemia has a positive correlation of average value with the level of parathyroid hormone, phosphorus and negative with the content of calcium before surgery. Conclusion. The decrease in the level of total calcium with the development of clinical symptoms occurs on 4-5 days after surgery for primary hyperparathyroidism in 6.7% and for secondary or tertiary - in 66.7%. Risk factors for hypocalcemia are the baseline low level of calcium and high of parathyroid hormone, phosphorus.

scholarly journals THE INFLUENCE OF NEW BEDDING MATERIAL ON MORPHOBIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS OF BLOOD OF GOSLINGS OF BROILERS

2021 ◽  
Vol 247 (3) ◽  
pp. 244-248
Author(s):  
V.G. Sofronov ◽  
◽  
R.N. Fayzrakhmanov ◽  
N.I. Danilova ◽  
E.L. Kuznetsova ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Allergic Reactions ◽  
Bedding Material ◽  
Total Calcium ◽  
Immunological Parameters ◽  
Mucous Membranes

The harmlessness of the new hygienic bedding material and the effect of broilers on the body have been studied. During the experiment, it was found that the new hygienic litter material, representing waste from the woodworking industry, treated with thermochemical and biotechnological methods, does not have an irritating effect on the skin, does not irritate the mucous membranes of the eyes and allergic reactions in rabbits, but, on the contrary, improves certain morphobiochemical and immunological parameters of the body’s blood are goslings. Ultimately, this was reflected in an increase in red blood cells by 7.04 %, hemoglobin – 5.20 %, total protein – 6.3 %, total calcium – 5.8 %, inorganic phosphorus – 5.1 %, glucose – 5,4 % compared with the control.

Chemical and clinical evaluation of the random access analyzer "RA-1000".

1984 ◽  
Vol 30 (3) ◽  
pp. 364-368 ◽  
Author(s):  
M K Schwartz ◽  
B E Statland ◽  
J Coughlin ◽  
C Eisen ◽  
M Fleisher ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Random Access ◽  
Inorganic Phosphorus ◽  
Production Model ◽  
Excellent Correlation ◽  
Gamma Glutamyltransferase

Abstract In the RA-1000, a random-access discrete analyzer, an inert fluorocarbon fluid is used to prevent interaction and carryover. Production-model instruments were evaluated in two laboratories with respect to determination of glucose, creatinine, total protein, inorganic phosphorus, cholesterol, alkaline phosphatase, lactate dehydrogenase, creatine kinase, gamma-glutamyltransferase, and aspartate and alanine aminotransferases. Within-run, among-run, and day-to-day (for 15 days) precision was assessed, and results were correlated with those obtained by the methods routinely in use in our departments. Precision was excellent, correlation acceptable.

scholarly journals Use of “Kayod” Preparation in Cows

2021 ◽  
Author(s):  
Yulia Luschai ◽  
Olga Dutova ◽  
Elena Shaganova ◽  
Irina Pleshakova ◽  
Elena Mashkina
Keyword(s):  
Metabolic Disorders ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Total Calcium ◽  
Single Use ◽  
Biogeochemical Provinces ◽  
Experimental Group ◽  
Subcutaneous Implant

The Altai territory is an agricultural region; it is a biogeochemical province deficient in iodine. We conducted a study of metabolic disorders in cows that had hypofunction of the thyroid gland. Our research is devoted to the expediency of a single use of the drug “Kayod” administered in the form of a subcutaneous implant in order to normalize the therapeutic status of animals suffering from a chronic lack of iodine in their diet. The purpose of this work is to justify not only the use of the preparation “Kayod” in animals kept in the biogeochemical provinces deficient in iodine, but also the use of tablets administered through a subcutaneous implant. We found a significant increase in the amount of hemoglobin and a decrease in the number of white blood cells in the blood of animals after the use of “Kayod”, and in the second experimental group, these indicators were slightly higher than in the first one. Indicators of the level of total protein and reserve alkalinity of blood were within the limits of physiological norms, but in the experimental groups they were slightly higher than in the control group. The level of total calcium and carotene in the blood of animals in the experimental groups, especially the second, significantly exceeded the level of the same substances in the blood of the animals in the control group. However, the level of inorganic phosphorus tended to decrease. The level of glucose in the blood of cows in the experimental group increased significantly more than in the blood of cows in the control group. This increase was particularly significant in the blood of animals in the second experimental group. When the preparation “Kayod” was used in the form of a subcutaneous implant, there were no clinical signs of hypothyroidism in the cows of the first experimental group after nine weeks. In the cows of the second experimental group, the condition of the cows returned to normal after six weeks. Keywords: Cows, iodine, hypothyroidism, implant, “Kayod

scholarly journals State of protein and mineral exchange in broiler-chickens for the use of Zinc and Mangane chelates

2019 ◽  
pp. 85-94
Author(s):  
V. Sakara ◽  
A. Melnyk ◽  
F. Markhenkov
Keyword(s):  
Total Protein ◽  
Broiler Chickens ◽  
Zinc Content ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Experimental Group

The results of the application of a complex of Zinc and Manganese chelates to protein, macro- and micro-mineral exchanges in the body of broiler chickens of the Cobb 500 cross-breed are presented. The administration of Zn and Mn chelates in a dose of 0,2 ml/l for chicken broilers of 23 days of age (1st experimental group) during 14 days influenced the increase in the total protein content to 35,3±0,8 g/l, which is 8 % higher compared with the control – 32,5±0,7 g/l (p<0,01 ) The concentration of total Calcium in serum of broiler chickens 37-day-old (end of trial) was higher in the first experimental group by 7,3 % (2,05±0,06 mmol/l) compared to control – 1,9±0,03 mmol/l. In the second group, where chelates were administered in a dose of 0,4 ml/l of water, the Calcium content was 2,0±0,02 mmol/l, which is 5 % more than the control group. The two-week application of Zn and Mn chelates also led to an increase in the level of inorganic Phosphorus in the serum of the first group to 2,6±0,12 mmol/l (+ 11,5 %) compared with the control group. After administration of chelates Zn and Mn at doses of 0,2 ml/l water, the Zinc content was higher by 4,3% (23,5±0,2 μmol/l) compared to control – 22,5±0,34 μmol/l (p<0,01). In the second group, where the dose of chelates was 0,4 ml/l, the concentration of Zinc was 23,6±0,16 μmol/l, which is more than 4,6 % for control (p<0,01). At the end of the study, the content of Manganese in serum of broiler chickens in the first group was 1,9±0,07 μmol/l (p<0,05), which is 10,4% higher compared with the control – 1,7±0,06 μmol/l (3rd sampling). The level of this element in the second group was 2,0±0,08 μmol/l (p<0,01), which is higher by 16,7 % relative to the control. At the end of the experiment, the coupon concentration in the first and second experimental groups was 12,6 and 9,3 % higher, compared with control, 7,0±0,37 μmol/l. In turn, the serum Ferrum content of the first group was greater by 9,3 % (19,3±0,69 μmol/l), and the second by 6,7 % (18,8±0,47 μmol/l) compared to the bird of the control group. Key words: chicken broilers, chelates, Zinc, Manganese, Cuprom, Ferrum, metabolism.

scholarly journals Effect of feeding chelates of trace elements on morphological and biochemical blood indicators of cows

2017 ◽  
Vol 19 (74) ◽  
pp. 119-122
Author(s):  
S. V. Kulibaba ◽  
M. M. Dolgaya ◽  
N. S. Emelyanova ◽  
G. O. Goncharenko
Keyword(s):  
Trace Elements ◽  
Total Protein ◽  
Blood Cells ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
Chelate Complexes ◽  
Animal Blood ◽  
Significant Difference ◽  
Experimental Group ◽  
Effect Of Feeding

In the article was discusses the questions about the effect of feeding chelate complexes of trace elements of copper (Cu), zinc (Zn), manganese (Mn) on morphological and biochemical blood indicators of cows of Ukrainian black mottled dairy breed on the second month of lactation. For the experiment, 40 cows were selected and formed four groups: one control and three experimental. The cows in the control group fed a premix of sulfate salts of Cu, Zn and Mn in doses that are 100% covered their lack in a forage. Experimental animals from I, II and III groups were fed premixes with chelate complexes of Cu, Zn and Mn, which offset the deficiency of trace elements in the feed ration for 100, 50 and 25% (calculated on pure element), respectively. In animal blood examined the number of red blood cells, white blood cells, hemoglobin, phagocytic activity of neutrophils, and in serum – total protein, albumin, globulin, glucose, total calcium, inorganic phosphorus, carotene, creatinine, urea, cholesterol, activity of AST, ALT and alkaline phosphatase. As a result studies, have shown that blood indicators of the animals were within physiological fluctuations. Found that in the serum of animals of III experimental group the contents of total protein was lowered by 4.1% (P < 0.05) than in the first, and by 3.6% (P < 0.1) relative to the second group. It was determined that the concentration of albumin and total calcium in serum of II experimental group of cows was higher by 4.6% and by 6.0% (P < 0.1), respectively, relative to the third group. Content of inorganic phosphorus in the blood serum of animals of II group was higher at trends over the indicator in the serum of animals of the control and first experimental groups by 8.1% and by 6.9%, respectively. According to other indicators of animals blood does not have significant difference.

Severe metabolic acidosis and disturbances of calcium metabolism induced by acetazolamide in patients on haemodialysis

1990 ◽  
Vol 78 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Sergio De Marchi ◽  
Emanuela Cecchin
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Metabolic Acidosis ◽  
Carbonic Anhydrase ◽  
Normal Control ◽  
Serum Calcium ◽  
Normal Subjects ◽  
Inorganic Phosphorus ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D

1. To investigate mechanisms of extrarenal buffering in uraemic acidosis, we studied the effects of the carbonic anhydrase inhibitor, acetazolamide, in normal subjects and in patients with end-stage kidney disease on maintenance haemodialysis with virtually no urine output. 2. Acetazolamide (500 mg) was administered daily for 7 days, after pretreatment for 1 month with 1,25-dihydroxyvitamin D (n = 12) or placebo (n = 12); only placebo was administered to a third group (n = 12) of haemodialysis patients. In addition, acetazolamide was administered to normal control subjects (n = 12). 3. Treatment with acetazolamide resulted in a more marked metabolic acidosis in haemodialysis patients than in normal control subjects and the effect in haemodialysis patients was attenuated by prior treatment with 1,25-dihydroxyvitamin D. 4. The administration of acetazolamide to haemodialysis patients led to an increase in serum inorganic phosphorus, bone isoenzyme of alkaline phosphatase and parathyroid hormone, and a reduction in serum calcium, whereas acetazolamide had no effect on these variables in normal subjects. In contrast, in the haemodialysis patients previously treated with 1,25-dihydroxyvitamin D, acetazolamide increased serum inorganic phosphorus, bone isoenzyme of alkaline phosphatase, parathyroid hormone and serum calcium. 5. We hypothesize that the metabolic acidosis induced by acetazolamide in haemodialysis patients may result from interference with the mechanisms of extrarenal buffering. 6. As parathyroid hormone, 1,25-dihydroxyvitamin D and carbonic anhydrase are thought to be involved in bone buffering, we suggest that the marked acidosis seen in haemodialysis patients treated with acetazolamide may be due to impaired parathyroid hormone-mediated bone buffering.

scholarly journals Вміст мінеральних компонентів у ротовій рідині дітей дошкільного віку з патологією опорно-рухового апарату

2017 ◽  
Author(s):  
N. I. Bodnaruk ◽  
T. Y. Lysak
Keyword(s):  
Alkaline Phosphatase ◽  
Dental Caries ◽  
Comparison Group ◽  
Dental Health ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Tooth Decay ◽  
Total Calcium ◽  
Mineral Components ◽  
Reduced Activity

Musculoskeletal disorders (MSD) occupy a leading place among the diseases affecting the dental health of children. The composition and properties of the oral fl uid are an appropriate refl ection of the various processes in the body and can help to study the pathogenesis of tooth decay associated with MSD.The aim of the study – to determine mineral ingredients composition of saliva in children with MSD and to identify its relationship with dental caries.Materials and Methods. 90 5-year old children were examined and divided into three groups of 30 persons in each: fi rst – children with dental caries and MSD; the second – children with dental caries, MSD and undifferentiated connective tissue disease (UCTD); the third – children with dental caries without somatic pathology (comparison group). The deft values and levels of caries prevalence were evaluated. Mineral components of the saliva were determined by photometric method.Results and Discussion. The deft value was (6.17±0.52) in children with MSD, (8.00±0.50) in children with MSD and UCTD and (4.37±0.47) in children of the comparison group. In children with MSD total calcium level in the saliva was 33.80 % lower compared to that in comparison group and 25.53 % higher than in children with MSD and UCTD. The concentration of inorganic phosphorus in the saliva of children with MSD was 29.73 % lower compared to that in comparison group and 15.71 % higher than in children with MSD and UCTD. The same pattern has shown the magnesium level. Alkaline phosphatase activity in the saliva of both groups of children with MSD was lower than in comparison group.Conclusions. In the saliva of children with dental caries and MSD the defi ciencies of total calcium, inorganic phosphorus and magnesium, as well as reduced activity of alkaline phosphatase were revealed.

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