scholarly journals In vitro apatite formation on organic polymers modified with a silane coupling reagent

2005 ◽  
Vol 2 (4) ◽  
pp. 335-340 ◽  
Author(s):  
Yuki Shirosaki ◽  
Masaaki Kubo ◽  
Seisuke Takashima ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
...  
Keyword(s):  
High Density Polyethylene ◽  
Body Fluid ◽  
Contact Angles ◽  
Organic Polymer ◽  
Apatite Formation ◽  
Distilled Water ◽  
Organic Polymers ◽  
Coupling Reagent ◽  
Silane Coupling

γ-Methacryloxypropyltrimethoxysilane (γ-MPS) was grafted to high-density polyethylene, polyamide and silicone rubber substrates by the emulsion polymerization procedure in order to provide these organic polymers with in vitro apatite-forming ability. The contact angles towards distilled water of the γ-MPS-grafted specimens were lower than those of the original organic polymer specimens, indicating that the grafted substrates were more hydrophilic. The in vitro apatite formation in a simulated body fluid (Kokubo solution) was confirmed for several of the γ-MPS-grafted specimens.

Apatite-Forming Ability of Organic-Inorganic Hybrids Prepared from Calcium Silicate and Glucomannan

2007 ◽  
Vol 361-363 ◽  
pp. 567-570
Author(s):  
Yasuyuki Morita ◽  
Toshiki Miyazaki ◽  
Eiichi Ishida ◽  
Chikara Ohtsuki
Keyword(s):  
Calcium Silicate ◽  
Body Fluid ◽  
Organic Polymer ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
Living Body ◽  
Bioactive Ceramics ◽  
Previously Treated

So-called bioactive ceramics are used for bone-repairing owing to attractive features such as direct bone-bonding in living body. However, there is limitation on clinical applications due to their inappropriate mechanical properties performances such as higher brittleness and lower fracture toughness than natural bone. To overcome this problem, hybrid materials have been developed by modification of calcium silicate, that is basic component of bioactive ceramics, with organic polymer. It is known that bioactive ceramics bond to bone through bone-like apatite layer which is formed on their surfaces by chemical reaction with body fluid. We attempted preparation of bioactive organic-inorganic hybrids from Glucomannan that is a kind of complex polysaccharide, and calcium silicate. Hybrids were prepared from glucomannan and tetraethoxysilane (TEOS). They were treated with 1M (=mol·m-3) CaCl2 aqueous solution for 24 hours. Then ability of apatite formation on the hybrids was examined in vitro using simulated body fluid (SBF, Kokubo solution). Surface structure of the specimens was examined by thin-film X-ray diffraction (TF-XRD), scanning electron microscopic (SEM) observation. The hybrids with TEOS:Glucomannan= 1:1 to 4:1 in mass ratio formed the apatite in SBF within 3 or 7 d, when they were previously treated with CaCl2 solution.

Bonelike Apatite Formation on Synthetic Organic Polymers and Fiber Coated with Titania

2007 ◽  
Vol 330-332 ◽  
pp. 679-682
Author(s):  
Jin Fang Liu ◽  
Satoshi Hayakawa ◽  
Kanji Tsuru ◽  
Jian Zhong Jiang ◽  
Akiyoshi Osaka
Keyword(s):  
Aqueous Solution ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Apatite Formation ◽  
Organic Polymers ◽  
In Vitro Bioactivity ◽  
Nylon Fiber ◽  
Apatite Deposition ◽  
Bonelike Apatite

Rutile films were deposited on polyethylene terephatalate (PET), polytetrafluoroethylene (PTFE), Silicone, poly6-caprolactam (Nylon6), polyhexamethylene adipamide (Nylon6,6) and Nylon fiber substrates using 0.03 M TiOSO4 and 0.03 M H2O2 aqueous solution at 80°C for 24 h. The rutile films exhibited excellent in vitro bioactivity as they induced apatite deposition in a simulated body fluid (SBF).

Surface Modification Of Polymers With Grafting And Coating Of Silane Hybrids And Their Bioactivity

1999 ◽  
Vol 576 ◽  
Author(s):  
Masaaki Kubo ◽  
Seisuke Takashima ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka ◽  
...  
Keyword(s):  
Contact Angle ◽  
High Density Polyethylene ◽  
Body Fluid ◽  
Chemical Species ◽  
Hydrated Silica ◽  
Bioactive Materials ◽  
Poly Vinyl Chloride ◽  
Essential Chemical ◽  
Surface Modified

ABSTRACTHydrated silica rich Si-OH and Si-0- groups serve in a body environment as sites for nucleation of apatite, and are known as an essential chemical species for bioactive materials. Organic polymers having surface modified with the hydrated silica will show bioactivity: bone tissues grow toward the apatite layer and bond to materials. Thus MOPS-M (3-methacryloxypropyltrimethoxysilane) was grafted under emulsion polymerization procedure to high density polyethylene (HDPE), poly (vinyl chloride) (PVC) and polyamide (PA) substrates to examine in vitro deposition of apatite (bioactivity) after soaking in a simulated body fluid (Kokubo solution). Bioactivity was confirmed for the grafted PVC and PA substrates and discussed in terms of contact angle and relative amount of grafted silane molecules.

Evaluation of surface modification techniques on the ability of apatite formation and corrosion behavior in synthetic body fluid: An in vitro study

2021 ◽  
Vol 22 ◽  
pp. 100866
Author(s):  
Cosmin M. Cotrut ◽  
Ionut C. Ionescu ◽  
Elena Ungureanu ◽  
Andrei Berbecaru ◽  
Raluca I. Zamfir ◽  
...  
Keyword(s):  
Surface Modification ◽  
Corrosion Behavior ◽  
Body Fluid ◽  
In Vitro Study ◽  
Vitro Study ◽  
Apatite Formation ◽  
Surface Modification Techniques

Apatite Formation on Porous TCP In Vitro: A Comparative Study between Static and Dynamic RSBF Systems

2007 ◽  
Vol 280-283 ◽  
pp. 1581-1584
Author(s):  
Chun Lin Deng ◽  
Ji Yong Chen ◽  
Yang Leng ◽  
Xin Long Wang ◽  
Yao Wu ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Comparative Study ◽  
Dynamic System ◽  
Tricalcium Phosphate ◽  
Body Fluid ◽  
Apatite Formation ◽  
Static System ◽  
Inner Pore

Porous tricalcium phosphate ceramics were immersed in static and dynamic revised simulated body fluid (RSBF) at 37°C. Morphology, composition and phase of precipitates on TCP were identified by SEM, FTIR and TEM methods. FTIR and TEM results indicated the deposits on the inner pore walls of TCP were OCP, and SEM results implied that the deposited way of precipitates in static system was different from that in dynamic system.

Heat Treatment Effect on Biological Behavior of Polyetheretherketone Composites

2022 ◽  
Vol 54 ◽  
pp. 119-128
Author(s):  
Alaa A. Mohammed ◽  
Jawad K. Oleiwi
Keyword(s):  
Heat Treatment ◽  
Simulated Body Fluid ◽  
Mtt Assay ◽  
Body Fluid ◽  
Antibacterial Properties ◽  
Biological Behavior ◽  
Apatite Formation ◽  
Crystalline Polymers ◽  
Density Methods

Polyetheretherketone is a semi-crystalline thermoplastic polymer, that so with heat treatments, it is possible to get different properties which are very important for the material performance. Heat treatment is a broadly utilized to develop the semi-crystalline polymers properties. In the present investigation, annealing of polyetheretherketone (PEEK) was carried out at temperatures above its glass transition temperature (Tg) to study its effects upon the biological conduct of the control and PEEK ternary composites. The bioactivity of the specimens was evaluated by investigating the apatite formation after immersion for different periods in a simulated body fluid (SBF). The biocompatibility of specimens was assessed by MTT assay. Additionally, the antibacterial property of the specimens versus S. aureus was observed with the optical density methods. The results manifested that the formation of hydroxyapatite was obviously observed on specimens after immersion for (7 and 14 days) in the simulated body fluid (SBF). Otherwise, the results of MTT assay recorded the PEEK specimens that excited the activity of fibroblasts, and therefore a high cytocompatibility was noticed and the specimens revealed antibacterial properties against S. aureus. So, the results of the bioactivity, biocompatibility and antibacterial tests in vitro demonstrated that the heat treatment enhanced biological behavior.

scholarly journals Improved bioactivity of GUMMETAL®, Ti59Nb36Ta2Zr3O0.3, via formation of nanostructured surfaces

2018 ◽  
Vol 9 ◽  
pp. 204173141877417 ◽  
Author(s):  
Shiva Kamini Divakarla ◽  
Seiji Yamaguchi ◽  
Tadashi Kokubo ◽  
Dong-Wook Han ◽  
Jae Ho Lee ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Cell Attachment ◽  
Cell Number ◽  
Calcium Titanate ◽  
Apatite Formation ◽  
X Ray ◽  
Nanostructured Surfaces ◽  
Implant Integration

The leading reason for implant revision surgery globally is lack of implant integration with surrounding bone. A new titanium alloy GUMMETAL® (Ti59Nb36Ta2Zr3O0.3) is currently used in biomedical devices and has a Young’s modulus that is better matched to bone. The surface was subject to NaOH, CaCl2, heat and water treatment (BioGum) after which the surfaces were evaluated using atomic force microscope, scanning electron microscope, X-ray diffractometer and elemental analysis using energy dispersive X-ray. To demonstrate enhanced bone bonding ability and cytocompatibility, apatite formation in simulated body fluid and in vitro stem cell attachment, proliferation and cytoskeleton organisation were examined. The formation of a ~200 nm nanoscale needle-like calcium titanate network on the surface following treatment was revealed and upon soaking in simulated body fluid, the formation of a ~5 µm layer of apatite. Metabolic activity of rat bone marrow stem cells on BioGum was increased in comparison to control and the cell number appeared greater, with more elongated morphology as early as 2 h post-seeding. This positions the modification as a simple and potentially universal technology for the improvement of implant integration.

Biocompatibility of CaSiO3 /High-Density Polyethylene Composites Prepared by Hot-Pressing

2006 ◽  
Vol 309-311 ◽  
pp. 1161-1164 ◽  
Author(s):  
Miho Tanuma ◽  
Yoshikazu Kameshima ◽  
Akira Nakajima ◽  
Kiyoshi Okada ◽  
Shigeo Asai ◽  
...  
Keyword(s):  
Hot Pressing ◽  
High Density Polyethylene ◽  
Ceramic Powder ◽  
High Density ◽  
Apatite Formation ◽  
Composite Surface ◽  
Ceramic Particles ◽  
Fast Bone ◽  
The Matrix

We have reported that CaSiO3 ceramics show very fast bone-like apatite formation in simulated body fluid (SBF). However, CaSiO3 ceramics have disadvantages in their mechanical properties and shapability. It is therefore more effective to develop composites of CaSiO3 particles dispersed in a matrix of polymer or metal. Such composites are usually prepared by homogeneously blending the ceramic powder with the matrix component. This method is, however, ineffective for the preparation of biocompatible polymers or metals because only the surfaces containing accidentally-exposed ceramic particles play a role in generating apatite in SBF. It is therefore necessary to add a large volume of ceramic powder and also to abrade the surface to expose more of the ceramic particles. In this study, CaSiO3/high-density polyethylene (HDPE) composites were prepared by hot-pressing to introduce surface CaSiO3 particles and their biocompatibilities were evaluated under in vitro conditions using SBF. CaSiO3 powders were spread on a HDPE plate and hot-pressed at 140oC and 4.9-14.7 MPa for 2 min. The composite sample (about 10×10×1 mm3 in size) was immersed in 30ml SBF (sample/solution ratio of 2.5 g/l) at 36.5oC. After 14 days soaking, the apatite product particles covered most of the composite surface and formed apatite layers. Bone-like apatite particles were formed only on the surface regions containing exposed CaSiO3 particles but no apatite was formed on the CaSiO3 particles buried in the HDPE matrix. The results show that this surface deposition method is very effective in developing biocompatibility in the composites using very small amounts of CaSiO3 powder (about <1 %v) compared with results reported for hydroxyapatite and AW glass-ceramic powders (requiring about 40 %v). It is also found that the inhomogeneous state of the CaSiO3 particles in the surface of the present composites strongly influences their biocompatibility. It will be necessary to improve the homogeneity of CaSiO3 dispersion in the surface of the composites to achieve a more uniform surface apatite layer.

Apatite-Forming Ability of Polyglutamic Acid Gel in Simulated Body Fluid: Effect of Cross-Linking Agent

2007 ◽  
Vol 330-332 ◽  
pp. 683-686 ◽  
Author(s):  
Atsushi Sugino ◽  
Toshiki Miyazaki ◽  
Chikara Ohtsuki
Keyword(s):  
Simulated Body Fluid ◽  
Heterogeneous Nucleation ◽  
Body Fluid ◽  
Organic Polymer ◽  
Carboxyl Groups ◽  
Cross Linking ◽  
Apatite Formation ◽  
Organic Substrates ◽  
Polyglutamic Acid ◽  
Acid Gel

Development of the organic-inorganic hybrids composed of apatite crystals and organic polymer is expected to be an attractive material that has mechanical properties similar to natural bone as well as bone-bonding ability, i.e. bioactivity. It is reported that the carboxyl groups (-COOH) on the surfaces of the organic substrates act as a catalyst for induction of heterogeneous nucleation of apatite. The present authors previously showed that the apatite was successfully deposited on the polyglutamic acid gels containing abundant carboxyl groups through the biomimetic process, when they were priorly treated with calcium chloride solution. In this study, we fabricated the polyglutamic acid gels with different degree of cross-linking. Effect of the cross-linking on their ability of the apatite formation was examined in simulated body fluid (SBF). It was suggested that the apatite deposition on the polyglutamic acid gels is governed not only by the amount of –COOH that induces the heterogeneous nucleation of the apatite, but also by swelling property that controls local increase in degree of supersaturation with respect to the apatite.

A Study of Bone-Like Apatite Formation on β-TCP/PLLA Scaffold in Static and Dynamic Simulated Body Fluid

2007 ◽  
Vol 330-332 ◽  
pp. 483-486
Author(s):  
Yun Qing Kang ◽  
Guang Fu Yin ◽  
Ke Feng Wang ◽  
Lin Luo ◽  
Li Liao ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Composite Scaffold ◽  
Pore Wall ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Good Ability ◽  
Ft Ir

The ability of apatite to form on the surface of biomaterials in simulated body fluid (SBF) has been widely used to predict the bone-bonding ability of bioceramic and bioceramic/polymer composites in vivo. Porous β-tricalcium phosphate/poly(L-lactic acid) (β-TCP/PLLA) composite scaffold was synthesized by new method. The ability of inducing calcium phosphate (Ca-P) formation was compared in static simulated body fluid(sSBF) and dynamic simulated body fluid (dSBF). The Ca-P morphology and crystal structures were identified using SEM, X-ray diffraction and Fourier transform infrared (FT-IR) spectroscopy. The results showed that the typical features of bone-like apatite formation on the surface and the inner pore wall of β-TCP/PLLA. Ca-P formation on scaffold surfaces in dSBF occurred slower than in sSBF and was more difficult with increasing flow rate of dSBF. The ability of apatite to form on β-TCP/PLLA was enhanced by effect of each other that has different degradable mechanism. Porous β-TCP/PLLA composite scaffold indicates good ability of Ca-P formation in vitro.

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