Fluctuations in the haemoglobin content of Daphnia

Pond-dwelling species of Daphnia are known to synthesize blood haemoglobin in poorly aerated natural w aters. It has now been found that a lake plankton species, the blood of which is colourless in nature, also synthesizes haemoglobin in artificial conditions of oxygen deficit. Haemoglobin formation in Daphnia , resulting from a low oxygen content of the water, increases with the amount of algal food eaten, up to a certain nutritional level. Chlorophyll in the food has not been found to favour haemoglobin synthesis. In the water of ponds harbouring red Daphnia we have found no factor stimulating haemo­globin synthesis other than oxygen deficit. The haemoglobin content of the blood of Daphnia varies within each instar. It is least when eggs are laid by the parthenogenetic female in her brood pouch and greatest when the eggs have developed into late-stage embryos ready to be released. After this, during the few hours before the moult and the laying of more eggs, the haemoglobin content of the blood decreases rapidly. At the same time the ovaries gain haemoglobin. This implies that haemoglobin passes from the blood into the ovaries shortly before the eggs are laid. During the development of the eggs in the brood pouch, the blood of the female gradually reacquires its full haemoglobin content.

It is known that Daphnia increases the haemoglobin content of its blood in water that has a low content of dissolved oxygen. It has now been found that a high temperature results in the synthesis of more haemoglobin than a low temperature, in waters of the same low oxygen content. The increased haemoglobin synthesis is probably due to the greater rate of metabolism of the animal at a high temperature and consequent lower oxygen concentration within the body, and to the lower oxygen affinity of haemoglobin at a high temperature resulting in a poorer oxygen supply to the tissues. It must also be due in part to the lower egg production at a high temperature and consequent lesser drain of haemoglobin from the blood into the eggs. Carbon dioxide is without influence on haemoglobin production. Duck faeces increase the haemoglobin synthesis in water of low oxygen content. This effect is not due to better nutrition. Vitamin B 12 causes a small increase in the amount of haemoglobin synthesized in response to oxygen deficiency. Iron salts added to water of low oxygen content can greatly increase the amount of haemoglobin synthesized. Ferrous salts are more effective than ferric salts. This is probably due to the ferrous ion producing ferric hydroxide in a more finely divided state than that produced by the ferric ion, so that the ferric hydroxide remains suspended in the water and available to be swallowed for a longer time. The rate of loss of haemoglobin by red animals transferred to aerated water has been studied.


2012 ◽  
Vol 727-728 ◽  
pp. 85-89
Author(s):  
Luzinete P. Barbosa ◽  
Elki C. Souza ◽  
Lucio Salgado ◽  
I. Costa

In this work, the effect of sintering atmosphere on the corrosion resistance of sintered titanium has been evaluated in 0.9 % aqueous NaCl solution to simulate physiological environment. Corrosion tests were performed on titanium porous sintered under vacuum and vacuum plus dynamic argon. The results showed better passive properties associated to the titanium sintered under argon plus vacuum atmosphere than to the vacuum sintered titanium. The better corrosion resistance of the argon plus vacuum sintered titanium was attributed to the formation of a thin passive film on the titanium surface during sintering due the low oxygen content present in this atmosphere.


1918 ◽  
Vol 27 (3) ◽  
pp. 399-412 ◽  
Author(s):  
H. G. Martin ◽  
A. S. Loevenhart ◽  
C. H. Bunting

Exposure of rabbits to an atmosphere of low oxygen content results in a stimulation of the cardiorespiratory systems, in an extension (hyperplasia) of red bone marrow and probably of a thyroid hyperplasia, with the further production of hydropic and hyaline degeneration in the cells of the parenchymatous organs. An atmosphere of high carbon dioxide and normal oxygen content produces, however, a stimulation of the cardiorespiratory systems, but no marrow extension and, in the concentrations used, but slight hydropic degeneration in the parenchyma of the glandular organs.


1939 ◽  
Vol 16 (3) ◽  
pp. 363-373 ◽  
Author(s):  
C. A. WINGFIELD

1. The oxygen consumption of normal and gill-less nymphs of the mayflies Baetis sp., Cloeon dipterum and Ephemera vulgata has been measured at various oxygen concentrations. 2. It has been found that over the complete range of oxygen concentrations studied, the tracheal gills do not aid oxygen consumption in Baetis sp. In Cloeon dipterum, at all oxygen concentrations tested, no gaseous exchange takes place through the gills; at low oxygen concentrations, however, the gills function as an accessory respiratory mechanism in ventilating the respiratory surface of the body and so aid oxygen consumption. In Ephemera Vulgata the gills aid oxygen consumption even at high oxygen concentrations. In this species the gills may function both as true respiratory organs and as a ventilating mechanism. 3. It is shown that the differences in gill function can be related to the oxygen content of the habitat of each species.


2020 ◽  
Vol 844 ◽  
pp. 9-23
Author(s):  
Sergii Gerasin ◽  
Dorota Kalisz ◽  
Jerzy Iwanciw

The current work deals the phenomenon of non-metallic inclusions as a result of the addition of Yttrium as an alloying component. The order of introducing individual components determines its final content in steel. This problem was analyzed using the WYK_Stal program developed at AGH-UST. Individual cases were considered using the accepted thermodynamics models based on Wagner’s formalism. The study of Y2O3 and Y2S3 phase precipitation and the relationship between the addition of Y, Al, Ca, O and S in molten steel was studied using the thermodynamic models. Based on the simulation, the authors stated that, the introduction of aluminum as the final deoxidizer into the liquid steel before the yttrium, results in the formation of non-metallic oxide inclusions. The low oxygen content in the metal bath promotes the formation of yttrium sulphide. In the case of calcium dosing, it is reasonable that, the yttrium is introduced after this element, which limits the losses on the formation of the yttrium sulphide phase.


Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1142 ◽  
Author(s):  
Iolanda Francolini ◽  
Elena Perugini ◽  
Ilaria Silvestro ◽  
Mariangela Lopreiato ◽  
Anna Scotto d’Abusco ◽  
...  

Tissue engineering is a highly interdisciplinary field of medicine aiming at regenerating damaged tissues by combining cells with porous scaffolds materials. Scaffolds are templates for tissue regeneration and should ensure suitable cell adhesion and mechanical stability throughout the application period. Chitosan (CS) is a biocompatible polymer highly investigated for scaffold preparation but suffers from poor mechanical strength. In this study, graphene oxide (GO) was conjugated to chitosan at two weight ratios 0.3% and 1%, and the resulting conjugates were used to prepare composite scaffolds with improved mechanical strength. To study the effect of GO oxidation degree on scaffold mechanical and biological properties, GO samples at two different oxygen contents were employed. The obtained GO/CS scaffolds were highly porous and showed good swelling in water, though to a lesser extent than pure CS scaffold. In contrast, GO increased scaffold thermal stability and mechanical strength with respect to pure CS, especially when the GO at low oxygen content was used. The scaffold in vitro cytocompatibility using human primary dermal fibroblasts was also affected by the type of used GO. Specifically, the GO with less content of oxygen provided the scaffold with the best biocompatibility.


2018 ◽  
Vol 452 ◽  
pp. 243-248 ◽  
Author(s):  
Masashi Matsuura ◽  
Tomoki Shiraiwa ◽  
Nobuki Tezuka ◽  
Satoshi Sugimoto ◽  
Tetsuya Shoji ◽  
...  
Keyword(s):  

2007 ◽  
Vol 303 (2) ◽  
pp. 391-394 ◽  
Author(s):  
Mingwang Shao ◽  
Hui Hu ◽  
Huizhao Ban ◽  
Min Li ◽  
Huazhong Gao

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