Identification and egg hatching activity of monohydroxy fatty acid eicosanoids in the barnacle Balanus balanoides

1992 ◽  
Vol 247 (1318) ◽  
pp. 41-46 ◽  
Keyword(s):  
Fatty Acid ◽  
Egg Hatching ◽  
Balanus Balanoides

Seasonal variations in the component fatty acid distributions of the lipids of Balanus balanoides

1972 ◽  
Vol 52 (4) ◽  
pp. 839-845 ◽  
Author(s):  
Doris Gardner ◽  
J. P. Riley
Keyword(s):  
Fatty Acid ◽  
Seasonal Variations ◽  
Marked Decrease ◽  
Early Spring ◽  
Component Fatty Acid ◽  
Lipid Fractions ◽  
Phospholipid Ratio ◽  
June July ◽  
Food Reserves ◽  
Balanus Balanoides

A study has been made of the seasonal variations in the component acids of the lipid fractions of Balanus balanoides. The relative proportions of the polyenoic acids in the triglycerides drop sharply during the winter. They reach a minimum in the early spring and then gradually rise to a maximum in the late autumn. These changes are largely balanced by increases in the saturated acids, principally 16:0 and 18 :o.1 During the winter there is a marked decrease in the triglyceride to phospholipid ratio as the depot fats are mobilised as food reserves. The proportions of the polyethenoid acids in the phospholipids decline slowly during the winter and spring and attain their minimum in June-July. This fall is mainly counterbalanced by increases in the amounts of saturated acids, principally 14:0 and 16:0. It seems likely that the observed decreases in the polyenoic acids are brought about by selective utilization of these compounds as reserves during the winter when food is scarce and depot lipid has been depleted.

scholarly journals In vitro anthelmintic and cytotoxic activities of extracts of Persea willdenovii Kosterm (Lauraceae)

2017 ◽  
Vol 92 (6) ◽  
pp. 674-680
Author(s):  
S.S. Santa Rosa ◽  
F.O. Santos ◽  
H.G. Lima ◽  
I.M.A. Reis ◽  
D.S.A. Cassiano ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Methyl Ester ◽  
Gastrointestinal Nematodes ◽  
Ethyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Egg Hatching ◽  
Trypan Blue Exclusion

AbstractThis study describes the effects of extracts and fractions of Persea willdenovii leaves against goat gastrointestinal nematodes and their cytotoxicity on Vero cells. The in vitro ovicidal and larvicidal activities of the crude ethanolic, hexane, ethyl acetate (EAE), butanolic and residual hydroethanolic extracts were assessed through the inhibition of egg hatching and larval motility assays. The most active extract (EAE) was then fractionated by chromatography in an open column containing silica gel, to furnish six fractions (Fr1–Fr6), which were also tested. The cytotoxicity of active extracts and fractions was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue exclusion assay. The EAE and two fractions (Fr1 and Fr2) showed inhibitory activity in the egg hatching of gastrointestinal nematodes of goats in a concentration-dependent manner. The effective concentrations for 50% inhibition (EC50) of egg hatching were 2.3, 0.12 and 2.94 mg/ml for EAE, Fr1 and Fr2, respectively. All extracts and fractions were not effective in inhibiting 50% of motility of infective larvae. EAE and Fr2 had IC50 values (50% inhibitory concentration) of 4.95 and 2.66 mg/ml, respectively. Fr1 showed a slight cytotoxic effect (cellular inviability <30%) only after 48 h of treatment (MTT test). Gas chromatography–mass spectrometry (GC–MS) analysis showed the presence of six fatty acid ethyl esters, a fatty acid methyl ester and a long-chain ketone in the most active fraction. These constituents identified in P. willdenovii can be related to the high ovicidal activity and relatively non-toxic effect of the extracts.

Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations

2018 ◽  
Vol 50 (2) ◽  
pp. 483-489 ◽  
Author(s):  
Yen‐Ju Pan ◽  
Anissa Souissi ◽  
Irina Sadovskaya ◽  
Jiang‐Shiou Hwang ◽  
Sami Souissi
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Cold Storage ◽  
Hatching Rate ◽  
Egg Hatching

Studies in the biochemistry of cirripede eggs: II. Changes in lipid composition during development of Balanus balanoides and B. Balanus.

1966 ◽  
Vol 46 (2) ◽  
pp. 249-261 ◽  
Author(s):  
R. M. C. Dawson ◽  
H. Barnes
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Content ◽  
Cellular Differentiation ◽  
Phosphatidyl Ethanolamine ◽  
Unsaturated Fatty Acids ◽  
Polar Material ◽  
Marine Animals ◽  
Balanus Balanoides ◽  
Late Stages

The eggs of two common cirripedes, Balanus balanoides and B. balanus, have been analysed at different stages of development for their lipid content.Triglyceride and phospholipid are the main lipid components; small amounts of free fatty acids, lipochromes, sterols, and some very non-polar material (possibly hydrocarbon) are also present.Lecithin followed by phosphatidyl ethanolamine make up a large proportion of the phospholipid fraction.The fatty-acid spectrum is similar to that of other marine animals being rich in unsaturated fatty acids of which eicosapentaenoic (20:5) and docosahexaenoic (22:6) predominate.Triglyceride is lost during development.Initially some lecithin and phosphatidyl ethanolamine are lost during development, but they accumulate in the late stages.Cardiolipin shows a marked increase in quantity during the late stages; this is presumably associated with increased cellular differentiation.There is a tendency for all the components to approach the values for the adult bodies—taken when lipid reserves are minimal—as the embryos develop.

GENETIC ANALYSIS OF A POPULATION OF TRIBOLIUM. V. VIABILITY OF THE UNSATURATED FATTY ACID SENSITIVE MUTANT

1975 ◽  
Vol 17 (4) ◽  
pp. 585-590 ◽  
Author(s):  
A. M. M. Scully ◽  
R. F. Costantino
Keyword(s):  
Fatty Acid ◽  
Relative Humidity ◽  
Unsaturated Fatty Acid ◽  
Corn Oil ◽  
Random Mating ◽  
Adult Emergence ◽  
Culture Conditions ◽  
Sensitive Mutant ◽  
Egg Hatching ◽  
Normal Genotype

The viability pattern of the unsaturated fatty acid sensitive mutant (cos/cos) of Tribolium castaneum was examined with reference to the normal genotype from egg hatching through adult emergence. The beetles were cultured on diets with 0, 3, or 5% corn oil in growth chambers maintained at 33 ± 1 °C and either 42 ± 6% or 75 ± 3% relative humidity. The pattern of the mutant was altered during the larval stage and was dependent on both the concentration of dietary fatty acid and the relative humidity of the culture chamber.Values of the viability component of fitness were assigned to the cos/cos, ±/cos and ±/± genotypes for multiple environments based on the number of eggs surviving to pupation. In large, random mating populations segregating at this locus, a stable genetic polymorphism is forecast. Predicted rates of change of allele frequency are dependent upon the culture conditions.

Identification and hatching factor activity of monohydroxyeicosapentaenoic acid in homogenates of the barnacle Elminius modestus

1988 ◽  
Vol 234 (1277) ◽  
pp. 455-461 ◽  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Positional Isomers ◽  
Factor Activity ◽  
Highly Active ◽  
Layer Chromatography ◽  
Balanus Balanoides

A crude eicosanoid extract of the barnacle Elminius modestus caused hatching of isolated egg masses from both E. modestus and Balanus balanoides . On thin-layer chromatography (TLC) two bands containing hatching factor activity were identified, a monohydroxy fatty acid (MHFA) band with potent hatching factor activity and a less active trihydroxy fatty acid (THFA) band. Gas chromatography-mass spectrometry (GC-MS) of the MHFA band showed that it consisted mainly of positional isomers of monohydroxyeicosapentaenoic acid, predominantly 8-hydroxyeicosapentaenoic acid (8-HEPE). A range of positional isomers of monohydroxyeicosapent­aenoic acid was produced by autoxidation of the methyl ester and this mixture was highly active in hatching E. modestus eggs. The commer­cially available stereoisomer 8( S )-HEPE hatched E. modestus eggs at concentrations as low as 10 -8 M. The saturated derivative 8-hydroxyeicosanoic acid did not show hatching factor activity.

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