GENETIC ANALYSIS OF A POPULATION OF TRIBOLIUM. V. VIABILITY OF THE UNSATURATED FATTY ACID SENSITIVE MUTANT

1975 ◽  
Vol 17 (4) ◽  
pp. 585-590 ◽  
Author(s):  
A. M. M. Scully ◽  
R. F. Costantino
Keyword(s):  
Fatty Acid ◽  
Relative Humidity ◽  
Unsaturated Fatty Acid ◽  
Corn Oil ◽  
Random Mating ◽  
Adult Emergence ◽  
Culture Conditions ◽  
Sensitive Mutant ◽  
Egg Hatching ◽  
Normal Genotype

The viability pattern of the unsaturated fatty acid sensitive mutant (cos/cos) of Tribolium castaneum was examined with reference to the normal genotype from egg hatching through adult emergence. The beetles were cultured on diets with 0, 3, or 5% corn oil in growth chambers maintained at 33 ± 1 °C and either 42 ± 6% or 75 ± 3% relative humidity. The pattern of the mutant was altered during the larval stage and was dependent on both the concentration of dietary fatty acid and the relative humidity of the culture chamber.Values of the viability component of fitness were assigned to the cos/cos, ±/cos and ±/± genotypes for multiple environments based on the number of eggs surviving to pupation. In large, random mating populations segregating at this locus, a stable genetic polymorphism is forecast. Predicted rates of change of allele frequency are dependent upon the culture conditions.

scholarly journals Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

Processes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 140 ◽  
Author(s):  
Nora Elfeky ◽  
Mostafa Elmahmoudy ◽  
Yongming Bao
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Rhodotorula Glutinis ◽  
Culture Conditions ◽  
Cellular Lipid ◽  
Fatty Acid Production ◽  
Carotenoid Production ◽  
Industrial Sectors ◽  
Dry Cell Weight ◽  
The Impact

The coproduction of lipid and carotenoid by red yeasts in one cycle is more convenient and economical for the industrial sectors, while the kinetics correlation between both products under different culture conditions has been scarcely studied. This study is aiming to correlate the impact of different carbon sources, carbon to phosphorus ratio (C/P), temperature, aeration, pH, and metals on dry cell weight, lipid (GC and fluorescence microscope), and carotenoid (HPLC) production by Rhodotorula glutinis, and applying a novel feeding approach using a 5 L bioreactor to enhance carotenoid and unsaturated fatty acid production by R. glutinis. Whatever the culture condition is, the reversible correlation between lipid and carotenoid production was detected. Remarkably, when adding 0.1 mM BaCl2, cellular lipid was significantly increased 14% more than the control, with 79.3% unsaturated fatty acid (46% C18:2 and C18:3) and 50% γ-carotene, while adding 1 mM NiSO4, cellular carotenoid was enhanced around 53% than the control (torulene 88%) with 81% unsaturated fatty acid (61% oleic acid). Excitingly, 68.8 g/l biomass with 41% cellular lipid (79% unsaturated fatty acid) and 426 µgpigment/gdcw cellular carotenoid (29.3 mg/L) (71% torulene) were obtained, when the pH-temperature dual controlled process combined with metallo-sulfo-phospho-glucose feeding approach in the 5 L bioreactor during the accumulation phase was conducted. This is the first study on the kinetic correlation between lipid and carotenoid under different C/P ratio and the dual effect of different metals like NiSO4 on lipid and carotenoid production by red oleaginous yeasts, which in turn significant for enhancing the coproduction of lipid and carotenoid by R. glutinis.

IDENTIFICATION AND CHARACTERIZATION OF A PURPLE NONSULFUR BACTERIUM ISOLATED FROM COASTAL AREA OF HAI PHONG FOR USING IN PRODUCTION OF UNSATURATED FATTY ACID (OMEGA 6, 7, 9)

2019 ◽  
Vol 57 (6) ◽  
pp. 665 ◽  
Author(s):  
Yen Thi Hoang ◽  
Quynh Thi Thu Tran ◽  
Ha Hoang Chu ◽  
Tuyen Thi Do ◽  
Thanh Tat Dang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Nitrogen Sources ◽  
16S Rrna Analysis ◽  
Rhodovulum Sulfidophilum ◽  
Purple Nonsulfur Bacterium ◽  
High Lipid ◽  
Physiological Properties ◽  
Omega 6

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.

TRIGLYCERIDE COMPOSITION OF SIXTEEN STRAINS OF MARINE DIATOM

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lily M.G. Panggabean ◽  
Abdullah Rasyid ◽  
Zarrah Duniani ◽  
Yana Meliana ◽  
Indah Kurniasih
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Double Bond ◽  
Unsaturated Fatty Acid ◽  
Ion Trap ◽  
Carbon Chain ◽  
Highly Unsaturated Fatty Acid ◽  
Marine Diatom ◽  
Ion Trap Mass Spectrometry ◽  
Spectra Analysis

Trigliceride or triacylglicerol (TAG) composition in crude oil of sixteen strain of marine diatom has been detected by spectra analyses on an Electrospray - Ion Trap – Mass Spectrometry (ESI-IT-MS) HCT Bruker-Daltonic GmbH instrument with AgNO3 used as coordination ionization agent. Biomass samples of each microalga strain were taken from early and late stationary cultures in f/2 enriched seawater and algal oils were extracted according to Bligh and Dyer. Results from spectra analysis showed that P-Pt-P (C16:0-C16:1-C16:0) were distinguished in TAG from diatom strains Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.1, Thalasiossira sp.2, Thalasiossira sp.3, Navicula sp. 1, Navicula sp. 2, Navicula sp. 3, Navicula sp. 4, Nitzschia sp. 2 and Amphora sp. In contrast, TAGs in Melosira sp. included P-P-P (C16:0-C16:0-C16:0) and P-P-O (C16:0-C16:0-C18:1) were identified. TAGs from Chaetoceros sp. were the most varies among samples, i.e. P-Pt-P (C16:0-C16:1-C16:0), A-P-M (C20:4-C16:0-C14:0), P-Pt-Lt (C16:0-C16:1-C18:3), P-Pt-A (C16:0-C16:1-C20:4), D-P-P (C22:6-C16:0-C16:0), A-Ln-P (C20:4-C18:2-C16:0). Various TAGs were also detected in Nitzschia sp.2, i.e. P-Pt-M (C16:0-C16:1-C14:0), P-Pt-P (C16:0-C16:1-C16:0), P-Pt-S (C16:0-C16:1-C18:0), P-Pt-A (C16:0-C16:1-C20:4). TAGs composition in Skeletonema strains that similar to those in Nitzschia sp.1 has longer carbon, i.e. P-P-O (C16:0-C16:0-C18:1), P-O-O (C16:0-C18:1-C18:1) and O-O-O (C18:1-C18:1-C18:1). TAGs with longer carbon chain and more double bond including highly unsaturated fatty acid C20:4 were increased with culture age in diatoms Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.2, Navicula sp.1 and Nitzschia sp. 2.Keywords: diatom, TAG, ESI-IT-MS, f/2, early and late stationary

QTL Analysis of 18-C Unsaturated Fatty Acid Contents in Zero-erucic Rape-seed (Brassica napus L.)

2011 ◽  
Vol 37 (8) ◽  
pp. 1342-1350 ◽  
Author(s):  
Yan-Yu YANG ◽  
Sheng-Qiang YANG ◽  
Zhe-Hong CHEN ◽  
Chun-Yun GUAN ◽  
She-Yuan CHEN ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Unsaturated Fatty Acid ◽  
Qtl Analysis ◽  
Rape Seed

scholarly journals Effect of gallic acid on the larvae of Spodoptera litura and its parasitoid Bracon hebetor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhay Punia ◽  
Nalini Singh Chauhan ◽  
Drishtant Singh ◽  
Anup Kumar Kesavan ◽  
Sanehdeep Kaur ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Spodoptera Litura ◽  
Negative Impact ◽  
Larval Mortality ◽  
Adult Emergence ◽  
Egg Hatching ◽  
Bracon Hebetor ◽  
Total Hemocyte Count ◽  
Glutathione S Transferases ◽  
Immense Potential

AbstractThe antibiosis effect of gallic acid on Spodoptera litura F. (Lepidoptera: Noctuidae) and its parasitoid evaluated by feeding six days old larvae on artificial diet incorporated with different concentrations (5 ppm, 25 ppm, 125 ppm, 625 ppm, 3125 ppm) of the phenolic compound revealed higher concentration (LC50) of gallic acid had a negative impact on the survival and physiology of S. litura and its parasitoid Bracon hebetor (Say) (Hymenoptera:Braconidae). The mortality of S. litura larvae was increased whereas adult emergence declined with increasing concentration of gallic acid. The developmental period was delayed significantly and all the nutritional indices were reduced significantly with increase in concentration. Higher concentration (LC50) of gallic acid adversely affected egg hatching, larval mortality, adult emergence and total development period of B. hebetor. At lower concentration (LC30) the effect on B. hebetor adults and larvae was non-significant with respect to control. Gene expression for the enzymes viz., Superoxide dismutase, Glutathione peroxidase, Peroxidase, Esterases and Glutathione S transferases increased while the total hemocyte count of S. litura larvae decreased with treatment. Our findings suggest that gallic acid even at lower concentration (LC30) can impair the growth of S. litura larvae without causing any significant harm to its parasitoid B. hebetor and has immense potential to be used as biopesticides.

Unsaturated fatty acid-tuned assembly of photosensitizers for enhanced photodynamic therapy via lipid peroxidation

2021 ◽  
Vol 334 ◽  
pp. 213-223
Author(s):  
Yanxian Hou ◽  
Qiang Fu ◽  
Yafei Kuang ◽  
Dan Li ◽  
Yixin Sun ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Photodynamic Therapy ◽  
Fatty Acid ◽  
Unsaturated Fatty Acid
Sign in / Sign up

Export Citation Format

Share Document