scholarly journals Growth medium composition-determined regulatory mechanisms are superimposed on CatR-mediated transcription from the pheBA and catBCA promoters in Pseudomonas putida

Microbiology ◽  
2001 ◽  
Vol 147 (8) ◽  
pp. 2149-2156 ◽  
Author(s):  
Andres Tover ◽  
Eve-Ly Ojangu ◽  
Maia Kivisaar
Keyword(s):  
Pseudomonas Putida ◽  
Growth Medium ◽  
Medium Composition ◽  
Regulatory Mechanisms

scholarly journals Influence of growth medium composition on synthesis of bioactive compounds and antioxidant properties of selected strains of Arthrospira cyanobacteria

2012 ◽  
Vol 30 (No. 3) ◽  
pp. 258-267 ◽  
Author(s):  
T. Tarko ◽  
A. Duda-Chodak ◽  
M. Kobus
Keyword(s):  
Growth Medium ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Beta Carotene ◽  
Medium Composition ◽  
The Other ◽  
Qualitative Composition ◽  
Efficient Synthesis ◽  
Selection Of ◽  
Carotene Synthesis

We studied how the selection of the growth medium influences the antioxidant properties and synthesis of bioactive compounds (β-carotene, C-phycocyanin, allophycocyanin, and phycoerythrin) in six selected species of cyanobacteria of Arthrospira genus. For this purpose, cyanobacteria cultures were cultivated on a typical Zarrouk medium and on a cheaper substitute – RM6 medium. Significant differences were observed in the efficiency of synthesis of the studied compounds depending on the strain of cyanobacteria. The quantitative and qualitative composition of Zarrouk medium was more beneficial for β-carotene synthesis in the cells of all strains of cyanobacteria studied. This medium also allowed for the antioxidant potential of the studied strains to be increased. On the other hand, the RM6 medium, deprived of some mineral ingredients, enabled more efficient synthesis of phycobiliproteins in all studied strains except A. platensis SAG 85.79.  

scholarly journals The effect of the growth medium composition on the fatty acids ofRhodothermus marinusand ‘Thermus thermosphilus’ HB-8

1993 ◽  
Vol 112 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Ana Paula Chung ◽  
Olga C. Nunes ◽  
Brian J. Tindall ◽  
Milton S. Costa
Keyword(s):  
Fatty Acids ◽  
Growth Medium ◽  
Medium Composition

scholarly journals Accumulation of Polyhydroxyalkanoate from Styrene and Phenylacetic Acid by Pseudomonas putida CA-3

2005 ◽  
Vol 71 (4) ◽  
pp. 2046-2052 ◽  
Author(s):  
Patrick G. Ward ◽  
Guy de Roo ◽  
Kevin E. O'Connor
Keyword(s):  
Pseudomonas Putida ◽  
Growth Medium ◽  
Carbon Concentration ◽  
Phenylacetic Acid ◽  
Nitrogen Concentration ◽  
Maximum Yield ◽  
Ammonium Phosphate ◽  
Fixed Carbon ◽  
Carbon To Nitrogen Ratio ◽  
Nitrogen Ratio

ABSTRACT Pseudomonas putida CA-3 is capable of converting the aromatic hydrocarbon styrene, its metabolite phenylacetic acid, and glucose into polyhydroxyalkanoate (PHA) when a limiting concentration of nitrogen (as sodium ammonium phosphate) is supplied to the growth medium. PHA accumulation occurs to a low level when the nitrogen concentration drops below 26.8 mg/liter and increases rapidly once the nitrogen is no longer detectable in the growth medium. The depletion of nitrogen and the onset of PHA accumulation coincided with a decrease in the rate of substrate utilization and biochemical activity of whole cells grown on styrene, phenylacetic acid, and glucose. However, the efficiency of carbon conversion to PHA dramatically increased once the nitrogen concentration dropped below 26.8 mg/liter in the growth medium. When supplied with 67 mg of nitrogen/liter, the carbon-to-nitrogen (C:N) ratios that result in a maximum yield of PHA (grams of PHA per gram of carbon) for styrene, phenylacetic acid, and glucose are 28:1, 21:1, and 18:1, respectively. In cells grown on styrene and phenylacetic acid, decreasing the carbon-to-nitrogen ratio below 28:1 and 21:1, respectively, by increasing the nitrogen concentration and using a fixed carbon concentration leads to lower levels of PHA per cell and lower levels of PHA per batch of cells. Increasing the carbon-to-nitrogen ratio above 28:1 and 21:1 for cells grown on styrene and phenylacetic acid, respectively, by decreasing the nitrogen concentration and using a fixed carbon concentration increases the level of PHA per cell but results in a lower level of PHA per batch of cells. Increasing the carbon and nitrogen concentrations but maintaining the carbon-to-nitrogen ratio of 28:1 and 21:1 for cells grown on styrene and phenylacetic acid, respectively, results in an increase in the total PHA per batch of cells. The maximum yields for PHA from styrene, phenylacetic acid, and glucose are 0.11, 0.17, and 0.22 g of PHA per g of carbon, respectively.

scholarly journals Increased lipids production of Nannochloropsis oculata and Chlorella vulgaris for biodiesel synthesis through the optimization of growth medium composition arrangement by using bicarbonate addition

2018 ◽  
Vol 154 ◽  
pp. 01009
Author(s):  
Dianursanti ◽  
Zenitha Lintang Agustin ◽  
Dwini Normayulisa Putri
Keyword(s):  
Growth Medium ◽  
Chlorella Vulgaris ◽  
Inorganic Carbon ◽  
Pilot Scale ◽  
Nannochloropsis Oculata ◽  
Medium Composition ◽  
Biodiesel Synthesis ◽  
Key Factor ◽  
Inorganic Carbon Source ◽  
Lipids Production

Chlorella vulgaris and Nannochloropsis oculata are a highly potential microalgae to be used in pilot-scale of biodiesel synthesis. The essential content from these microalgae is the fatty acid of lipid which is the main target for the feed and biodiesel industries. One of the key factor in improving lipid microalgae are the arrangemment of nutrients in the growth medium. Research on the regulation of nutrients using bicarbonate (HCO3-) as an additional inorganic carbon source has been done by many studies, but the yield of lipids obtained has not been much. The aim of the study was to improve the lipid yield of Chlorella vulgaris and Nannochloropsis oculata. Variation of [HCO3-] which added to Walne medium were 25 ppm and 75 ppm, while the Walne medium without the addition of bicarbonate acts as control. The results showed that [HCO3-] 75 ppm could increase Chlorella vulgaris biomass by 0.9162 g/l with 17.0% wt, while Nannochloropsis oculata produced the greatest lipid content in [HCO3-] 25 ppm of 20.3% wt and the largest biomass on [HCO3-] 75 ppm of 1.7233 g/l.

Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2

1995 ◽  
Vol 41 (9) ◽  
pp. 776-784 ◽  
Author(s):  
Xiuying Sun ◽  
Marilyn Griffith ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Medium ◽  
Polyacrylamide Gel Electrophoresis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Antifreeze Protein ◽  
High Arctic ◽  
Major Protein ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth promoting rhizobacterium Pseudomonas putida GR12-2 was originally isolated from the rhizosphere of plants growing in the Canadian High Arctic. Here we report that this bacterium was able to grow and promote root elongation of both spring and winter canola at 5 °C, a temperature at which only a relatively small number of bacteria are able to proliferate and function. In addition, the bacterium survived exposure to freezing temperatures, i.e., −20 and −50 °C. In an effort to determine the mechanistic basis for this behaviour, it was discovered that following growth at 5 °C, P. putida GR12-2 synthesized and secreted to the growth medium a protein with antifreeze activity. Analysis of the spent growth medium, following concentration by ultrafiltration, by SDS-polyacrylamide gel electrophoresis revealed the presence of one major protein with a molecular mass of approximately 32–34 kDa and a number of minor proteins. However, at this point it is not known which of these proteins contains the antifreeze activity.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, antifreeze protein.

Influence of carbohydrates on cell properties of Lactobacillus rhamnosus

2010 ◽  
Vol 5 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Jelena Begovic ◽  
Djordje Fira ◽  
Amarela Terzic-Vidojevic ◽  
Ljubisa Topisirovic
Keyword(s):  
Growth Medium ◽  
Lactobacillus Rhamnosus ◽  
Cell Surface Hydrophobicity ◽  
Protein Composition ◽  
Surface Hydrophobicity ◽  
Cellular Level ◽  
Medium Composition ◽  
Producer Strain ◽  
Carbohydrate Source ◽  
Complex Relationships

AbstractLactobacilli represent normal commensals of the human body, particularly in the gut and vagina where they protect these environments from incoming pathogens via a variety of mechanisms. The influence of the carbohydrate source present in reconstituted MRS growth medium on the different cell properties of two Lactobacillus rhamnosus strains were examined. Two human vaginal isolates, BGHV719 and exopolysaccharide producer strain BGHV954 were analyzed. The results demonstrated that unlike in reconstituted MRS with glucose as a carbon source, the presence of fructose, mannose, or rhamnose, significantly reduced cell surface hydrophobicity of both strains. In addition, differences in cell wall protein composition of L. rhamnosus BGHV719 and alterations in colony mucoidity of L. rhamnosus BGHV954 were also demonstrated. Light and SEM microscopy revealed differences on the cellular level when BGHV719 was cultivated in the presence of different sugars. The results of this study point out the importance of complex relationships between growth medium composition and the different aspects of bacterial behavior, and call for more detailed analyses of versatile bacterial responses to the changes in the environment, including vaginal ecosystem. This is especially important since lactobacilli are amongst the most widely used of probiotics.

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