scholarly journals Dengue 2 serotype and yellow fever coinfection

2021 ◽  
Vol 3 (12) ◽  
Author(s):  
Flávia Gehrke ◽  
Katharyna Cardoso Gois ◽  
Beatriz da Costa Alves Aguiar Reis ◽  
Gabriel Zorello Laporta ◽  
Fernando Luiz Affonso Fonseca
Keyword(s):  
Clinical Diagnosis ◽  
Yellow Fever ◽  
Laboratory Tests ◽  
Yellow Fever Virus ◽  
Genetic Material ◽  
High Sensitivity ◽  
Multiplex Assay ◽  
Case Presentation ◽  
Molecular Tests ◽  
The Family

Case Presentation. Arboviruses primarily consist of RNA, which favours greater genetic plasticity, with a higher frequency of mutations that allow the virus to adapt to different hosts. The initial symptomatology is nonspecific, in that the patient can present fever, myalgia, arthralgia, rash and headache. This makes a clinical diagnosis using laboratory tests difficult and time-consuming. In Brazil, the main arboviruses involved in epidemics belong to the family Flaviviridae. The patient in this case is from the municipality of São Bernardo do Campo, an area endemic for arboviruses. He presented symptoms of fever, myalgia and headache. Results. The multiplex assay for arboviruses detected genetic material from the dengue 2 and yellow fever viruses. Conclusion. This result confirms the importance of molecular tests showing high sensitivity and specificity that can assist clinical diagnosis, particularly in endemic areas during periods of outbreak for other arboviruses, like the epidemiological picture in Brazil in 2018, when significant co-circulation of dengue virus and yellow fever virus occurred. The presence of co-circulating arboviruses increases the chance of coinfection and demonstrates the importance of differential diagnosis.

scholarly journals Comparison of the performance of laboratory tests in the diagnosis of feline infectious peritonitis

2018 ◽  
Vol 30 (3) ◽  
pp. 459-463 ◽  
Author(s):  
Angelica Stranieri ◽  
Alessia Giordano ◽  
Saverio Paltrinieri ◽  
Chiara Giudice ◽  
Valentina Cannito ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Peripheral Blood ◽  
Laboratory Tests ◽  
Gene Sequencing ◽  
Body Cavity ◽  
Likelihood Ratios ◽  
Feline Infectious Peritonitis ◽  
S Gene ◽  
Molecular Tests ◽  
Sensitivity Specificity

We compared the performance of clinicopathologic and molecular tests used in the antemortem diagnosis of feline infectious peritonitis (FIP). From 16 FIP and 14 non-FIP cats, we evaluated retrospectively the sensitivity, specificity, and likelihood ratios (LRs) of serum protein electrophoresis, α1-acid glycoprotein (AGP) on peripheral blood, screening reverse-transcription nested PCR (RT-nPCR) on the 3’–untranslated region (3’-UTR), and spike (S) gene sequencing on peripheral blood, body cavity effusions, and tissue, as well as body cavity cytology and delta total nucleated cell count (ΔTNC). Any of these tests on blood, and especially the molecular tests, may support or confirm a clinical diagnosis of FIP. A negative result does not exclude the disease except for AGP. Cytology, 3’-UTR PCR, and ΔTNC may confirm a clinical diagnosis on effusions; cytology or 3’-UTR PCR may exclude FIP. Conversely, S gene sequencing is not recommended based on the LRs. On tissues, S gene sequencing is preferable when histology is highly consistent with FIP, and 3’-UTR PCR when FIP is unlikely. Combining one test with high LR+ with one with low LR− (e.g., molecular tests and AGP on blood, ΔTNC and cytology in effusions) may improve the diagnostic power of the most used laboratory tests.

scholarly journals High-Sensitivity Detection and Genotyping of Yellow Fever Virus

IBJ Plus ◽  
2021 ◽  
Author(s):  
◽  
Mario Di Donato ◽  
Riccardo De Santis ◽  
Giovanni Faggioni ◽  
Florigio Lista
Keyword(s):  
Yellow Fever ◽  
Yellow Fever Virus ◽  
High Sensitivity ◽  
Fever Virus ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

scholarly journals Zika virus infection: a public health emergency!

2017 ◽  
Vol 3 (3) ◽  
pp. 68
Author(s):  
Muhammad Salman Haider Qureshi ◽  
Bakhtawar Wajeeha Qureshi ◽  
Ramsha Khan
Keyword(s):  
Public Health ◽  
West Nile Virus ◽  
Virus Infection ◽  
Dengue Virus ◽  
Yellow Fever ◽  
Zika Virus ◽  
Yellow Fever Virus ◽  
Public Health Emergency ◽  
Human Pathogens ◽  
The Family

<p class="abstract"><em>Zika virus</em> belongs to the family of Flaviviridae. The Flaviviridae family also includes other human pathogens like <em>West Nile virus</em> (WNV), <em>Yellow fever virus</em> (YFV), mosquito transmitted <em>Dengue virus</em> (DENV), <em>Tick borne encephalitic virus</em> (TBEV) and <em>Japanese encephalitis virus</em> (JEV). <em>Zika virus</em> is a mosquito-borne disease and is transmitted by <em>Aedes aegypti</em> mosquito<span lang="EN-IN">. </span></p>

scholarly journals Flaviviruses – new human pathogens

2018 ◽  
Vol 72 ◽  
pp. 184-191
Author(s):  
Martyna Biała ◽  
Bartosz Jerczak ◽  
Małgorzata Inglot ◽  
Brygida Knysz
Keyword(s):  
Yellow Fever ◽  
Yellow Fever Virus ◽  
Clinical Manifestations ◽  
Health Impact ◽  
Human Populations ◽  
Human Pathogens ◽  
Tick Borne Encephalitis ◽  
Animal Pathogens ◽  
The Family ◽  
Significant Public Health

The family Flaviviridae includes arthropod-borne viruses that are transmitted to vertebrates by infected mosquitoes and ticks. There are over a 100 species in this family, most of which are animal pathogens, although it cannot be excluded that some animal flaviviruses will also cause human disease. The family name is due to the discovery of the yellow fever virus and is derived from the Latin word flavus, meaning yellow. Several human-pathogenic flaviviruses have a significant public health impact in different parts of the world and the potential of emerging in previously non-endemic regions. The medically important flaviviruses include among others: dengue, Zika, yellow fever, tick‐borne encephalitis (TBE) and West Nile viruses. Diseases that are caused by these viruses include a spectrum of illnesses ranging from asymptomatic infections to severe and fatal disease. Although the characteristics of these viruses are well defined, they are still unpredictable with increases in disease severity, unusual clinical manifestations, unexpected methods of transmission, long-term persistence, and the discovery of new species. This review compares the epidemiological and clinical features of the medically important flaviviruses and considers the effect of human activity on their evolution and dispersal. Climate change, urbanization and increasing ease of travel have created opportunities for the vector to spread and expand into new human populations. The combination of these factors makes it possible for the family Flaviviridae to change and emerge quickly as a new, potential human pathogen.

scholarly journals Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 203
Author(s):  
María Gemma Millán de la Blanca ◽  
Eva Martínez-Nevado ◽  
Cristina Castaño ◽  
Juncal García ◽  
Berenice Bernal ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genetic Material ◽  
First Year ◽  
Sperm Cryopreservation ◽  
Straight Line ◽  
The Family ◽  
Second Year ◽  
American Flamingo ◽  
Phoenicopterus Ruber

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.

scholarly journals Yellow fever virus outbreak in Brazil under current and future climate

2021 ◽  
Vol 6 ◽  
pp. 664-677
Author(s):  
Tara Sadeghieh ◽  
Jan M. Sargeant ◽  
Amy L. Greer ◽  
Olaf Berke ◽  
Guillaume Dueymes ◽  
...  
Keyword(s):  
Yellow Fever ◽  
Yellow Fever Virus ◽  
Fever Virus ◽  
Future Climate

scholarly journals Primary Congenital Lymphedema with More Than 10 Years of Treatment Using the Godoy Method Through to Adolescence

2021 ◽  
Vol 13 (1) ◽  
pp. 91-94
Author(s):  
Ana Carolina Pereira de Godoy ◽  
Jose Maria Pereira de Godoy ◽  
Maria de Fatima Guerreiro Godoy
Keyword(s):  
Treatment Period ◽  
Clinical Diagnosis ◽  
Treatment Option ◽  
Therapeutic Option ◽  
The Body ◽  
The Family ◽  
Cervical Stimulation

Introduction: Lymphedema is a specific type of edema that affects regions of the body in a chronic, progressive manner. Aim: The aim of the present study was to describe the therapeutic evolution of more than ten years of treatment for primary congenital lymphedema using the Godoy and Godoy method. Method: Ten children with primary congenital lymphedema with more than ten years of treatment at the Godoy Clinic were evaluated. Children with a clinical diagnosis of primary congenital lymphedema in treatment for more than 10 years with the Godoy Method. Cervical stimulation is the first treatment option of the method and is performed as monotherapy. The patients were reevaluated with weekly, bi-weekly and monthly frequencies and then every three months or when the family was able to return to the clinic. Results: For cases in which cervical stimulation was not possible, grosgrain stockings as monotherapy was the second therapeutic option. Conclusion: The Godoy and Godoy Method is effective at reducing edema in cases of primary congenital lymphedema, with the maintenance of the results throughout the treatment period.

scholarly journals Spondylocarpotarsal synostosis syndrome due to a novel loss of function FLNB variant: a case report

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Samina Yasin ◽  
Outi Makitie ◽  
Sadaf Naz
Keyword(s):  
Short Stature ◽  
Autosomal Recessive ◽  
Autosomal Recessive Disorder ◽  
Loss Of Function ◽  
Case Presentation ◽  
Pathogenic Variants ◽  
Skeletal Malformations ◽  
Tarsal Bones ◽  
The Family ◽  
Heterozygous Carriers

Abstract Background Loss of function or gain of function variants of Filamin B (FLNB) cause recessive or dominant skeletal disorders respectively. Spondylocarpotarsal synostosis syndrome (SCT) is a rare autosomal recessive disorder characterized by short stature, fused vertebrae and fusion of carpal and tarsal bones. We present a novel FLNB homozygous pathogenic variant and present a carrier of the variant with short height. Case presentation We describe a family with five patients affected with skeletal malformations, short stature and vertebral deformities. Exome sequencing revealed a novel homozygous frameshift variant c.2911dupG p.(Ala971GlyfsTer122) in FLNB, segregating with the phenotype in the family. The variant was absent in public databases and 100 ethnically matched control chromosomes. One of the heterozygous carriers of the variant had short stature. Conclusion Our report expands the genetic spectrum of FLNB pathogenic variants. It also indicates a need to assess the heights of other carriers of FLNB recessive variants to explore a possible role in idiopathic short stature.

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