scholarly journals Marinomonas alcarazii sp. nov., M. rhizomae sp. nov., M. foliarum sp. nov., M. posidonica sp. nov. and M. aquiplantarum sp. nov., isolated from the microbiota of the seagrass Posidonia oceanica

2011 ◽  
Vol 61 (9) ◽  
pp. 2191-2196 ◽  
Author(s):  
Patricia Lucas-Elío ◽  
Ester Marco-Noales ◽  
Elena Espinosa ◽  
Mónica Ordax ◽  
María M. López ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Dna Hybridization ◽  
Novel Species ◽  
Posidonia Oceanica ◽  
Major Fatty Acid ◽  
Bacterial Strains ◽  
16S Rrna Sequence ◽  
Minor Fatty Acid ◽  
16S Rrna Sequence Analysis ◽  
Fatty Acid Compositions

Five novel Gram-reaction-negative aerobic marine bacterial strains with DNA G+C contents <50 mol% were isolated from the seagrass Posidonia oceanica. 16S rRNA sequence analysis indicated that they belonged to the genus Marinomonas. Major fatty acid compositions, comprising C10 : 0 3-OH, C16 : 0, C16 : 1ω7c and C18 : 1ω7c, supported the affiliation of these strains to the genus Marinomonas. Strains IVIA-Po-14bT, IVIA-Po-145T and IVIA-Po-155T were closely related to Marinomonas pontica 46-16T, according to phylogenetic analysis. However, DNA–DNA hybridization values <35 % among these strains revealed that they represented different species. Further differences in the phenotypes and minor fatty acid compositions were also found among the strains. Another two strains, designated IVIA-Po-181T and IVIA-Po-159T, were found to be closely related to M. dokdonensis DSW10-10T but DNA–DNA relatedness levels <40 % in pairwise comparisons, as well as some additional differences in phenotypes and fatty acid compositions supported the creation of two novel species. Accordingly, strains IVIA-Po-14bT ( = CECT 7730T  = NCIMB 14671T), IVIA-Po-145T ( = CECT 7377T  = NCIMB 14431T), IVIA-Po-155T ( = CECT 7731T  = NCIMB 14672T), IVIA-Po-181T ( = CECT 7376T  = NCIMB 14433T) and IVIA-Po-159T ( = CECT 7732T  = NCIMB 14673T) represent novel species, for which the names Marinomonas alcarazii sp. nov., Marinomonas rhizomae sp. nov., Marinomonas foliarum sp. nov., Marinomonas posidonica sp. nov. and Marinomonas aquiplantarum sp. nov. are proposed, respectively.

scholarly journals Pseudomonas eucalypticola sp. nov., a producer of antifungal agents isolated from Eucalyptus dunnii leaves

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yujing Liu ◽  
Zhang Song ◽  
Hualong Zeng ◽  
Meng Lu ◽  
Weiyao Zhu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Antifungal Agents ◽  
Novel Species ◽  
Phytopathogenic Fungi ◽  
Strictly Aerobic ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Eucalyptus Dunnii ◽  
16S Rrna Sequence Analysis

AbstractPseudomonas are ubiquitously occurring microorganisms and are known for their ability to produce antimicrobials. An endophytic bacterial strain NP-1 T, isolated from Eucalyptus dunnii leaves, exhibits antifungal properties against five tested phytopathogenic fungi. The strain is a Gram-negative rod-shaped bacterium containing a single polar flagellum. It is strictly aerobic, grows at 4–37 °C, 2–5% NaCl, and pH 3–7. The 16S rRNA sequence analysis showed that NP-1 T belongs to the Pseudomonas genus. Phylogenetic analysis based on four concatenated partial genes (16S rDNA, gyrB, rpoB and rpoD) and the phylogenomic tree indicated that NP-1 T belongs to Pseudomonas fluorescens lineage but is distinct from any known Pseudomonas species. The G + C mol % of NP-1 T genome is 63.96, and the differences between NP-1 T and related species are larger than 1. The digital DNA-DNA hybridization and tetranucleotide signatures are 23.8 and 0.97, which clearly separates strain NP-1 T from its closest neighbours, Pseudomonas coleopterorum and Pseudomonas rhizosphaerae. Its phenotypic and chemotaxonomic features confirmed its differentiation from related taxa. The results from this polyphasic approach support the classification of NP-1 T as a novel species of Pseudomonas, and the name of Pseudomonas eucalypticola is thus proposed for this strain, whose type is NP-1 T (= CCTCC M2018494T = JCM 33572 T).

scholarly journals Arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol-degrading actinobacterium

2004 ◽  
Vol 54 (3) ◽  
pp. 773-777 ◽  
Author(s):  
Ludmila Kotoučková ◽  
Peter Schumann ◽  
Eva Durnová ◽  
Cathrin Spröer ◽  
Ivo Sedláček ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Single Species ◽  
Growth Cycle ◽  
Nitroaromatic Compounds ◽  
Bacterial Isolates ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Arthrobacter Aurescens ◽  
16S Rrna Sequence Analysis

Three bacterial isolates from soil, capable of degradation or transformation of nitroaromatic compounds and displaying a rod–coccus growth cycle, were studied by a polyphasic approach. On the basis of 16S rRNA sequence analysis and of chemotaxonomic characteristics, such as type A3α peptidoglycan with an interpeptide bridge Ala–Thr–Ala, the major menaquinone MK-9(H2) and fatty acid composition, the isolates were assigned to the genus Arthrobacter. DNA–DNA hybridization, riboprinting and phenotypic studies revealed that the three strains constitute a single species, distinct from phylogenetically neighbouring Arthrobacter aurescens and Arthrobacter ilicis. A novel species, Arthrobacter nitroguajacolicus sp. nov., with the type strain G2-1T (=CCM 4924T=DSM 15232T) is proposed.

scholarly journals Streptomyces gilvifuscus sp. nov., an actinomycete that produces antibacterial compounds isolated from soil

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3493-3500 ◽  
Author(s):  
Tuan Manh Nguyen ◽  
Jaisoo Kim
Keyword(s):  
Fatty Acids ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Gram Stain ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Antibacterial Compounds ◽  
Cellular Fatty Acids ◽  
16S Rrna Sequence Analysis

This study describes a novel actinomycete, designated T113T, which was isolated from forest soil in Pyeongchang-gun, Republic of Korea, and is an aerobic, Gram-stain-positive actinobacterium that forms flexibilis chains of smooth, elliptical or short rod-shaped spores. The results of 16S rRNA sequence analysis indicated that strain T113T exhibited high levels of similarity to previously characterized species of the genus Streptomyces (98.19–98.89 %, respectively). However, the results of phylogenetic and DNA–DNA hybridization analyses confirmed that the organism represented a novel member of the genus Streptomyces. Furthermore, using chemotaxonomic and phenotypic analyses it was demonstrated that the strain exhibited characteristics similar to those of other members of the genus Streptomyces. The primary cellular fatty acids expressed by this strain included anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. While diphosphatidylglycerol and phosphatidylethanolamine were the predominant lipids expressed by strain T113T, moderate amounts of phosphatidylinositol and phosphatidylinositol mannoside were also detected. Whole-cell hydrolysates contained glucose and ribose, and the predominant menaquinone detected was MK-9 (H6); however, moderate amounts of MK-9 (H8) and trace amounts of MK-10 (H2) and MK-10 (H4) were also detected. We therefore propose that strain T113T be considered as representing a novel species of the genus Streptomyces and propose the name Streptomyces gilvifuscus sp. nov. for this species, with strain T113T ( = KEMB 9005-213T = KACC 18248T = NBRC 110904T) being the type strain.

scholarly journals Janthinobacterium tructae sp. nov., Isolated from Kidney of Rainbow Trout (Oncorhynchus mykiss)

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 229
Author(s):  
Won Joon Jung ◽  
Sang Wha Kim ◽  
Sib Sankar Giri ◽  
Hyoun Joong Kim ◽  
Sang Guen Kim ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Dna Hybridization ◽  
Biochemical Analysis ◽  
Major Fatty Acid ◽  
Phylogenetic Study ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Close Relatives ◽  
Rrna Sequences ◽  
Fatty Acid Compositions ◽  
Genome Distance

This study presents a novel Janthinobacterium strain, SNU WT3, isolated from the kidney of rainbow trout. A phylogenetic study using 16S rRNA sequences indicated that the strain is closely related to Janthinobacterium svalbardensis JA-1T. However, biochemical analysis found differences in D-xylose adonitol, N-acetylglucosamine, arbutin, and cellobiose. As for genome-to-genome distance and average nucleotide identity values calculated between strain SNU WT3 and other related strains such as J. lividum EIF1, J. svalbardensis PAMC 27463, and J. agaricidamnosum BHSEK were all below the cutoff value between species. DNA-DNA hybridization between strain SNU WT3 and other close relatives indicated the results of J. lividum DSM 1522T (47.11%) and J. svalbardensis JA-1T (38.88%) individually. The major fatty acid compositions of strain SNU WT3 were cylco-C17:0 (41.45%), C16:0 (33.86%) and C12:0 (5.87%). The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, and diphosphatidylglycerol. The quinone system was composed mainly of ubiquinone Q-8. The genome of strain SNU WT3 consists of 6,314,370 bp with a G + C content of 62.35%. Here, we describe a novel species of the genus Janthinobacterium, and the name Janthinobacterium tructae has been proposed with SNU WT3T (=KCTC 72518 = JCM 33613) as the type strain.

Safflower Oil: Physical and Chemical Properties, and Fatty Acid Composition

1964 ◽  
Vol 47 (4) ◽  
pp. 776-780
Author(s):  
Walld Ibrahim ◽  
John Iverson ◽  
David Firestone
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Chemical Properties ◽  
Major Fatty Acid ◽  
Safflower Seed ◽  
Physical And Chemical Properties ◽  
Minor Fatty Acid ◽  
Physical And Chemical ◽  
And Chemical Properties

Abstract Some physical and chemical properties, including fatty acid composition by gas chromatography, of 15 commercially refined safflower oils and three crude oils extracted from safflower seed, were determined. The average and standard deviations found were: refractive index, 1.4748 ± 0.0003; specific gravity, 0.9210 ± 0.0005; iodine number, Wijs, 143.4 ± 1.4; squalene, 7.1 mg/100 g ± 1.1; and Bellier test, 7.4° ± 0.9. The major fatty acid components were; palmitic 6.7 ± 0.3, stearic 2.7 ± 0.4, oleic 12.9 ± 0.6, and linoleic 77.5 ± 1.1. Minor fatty acid components detected were myristic, arachidic, and eicosenoic acids.

scholarly journals Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 625-632 ◽  
Author(s):  
Javier Pascual ◽  
Marina García-López ◽  
Gerald F. Bills ◽  
Olga Genilloud
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Novel Species ◽  
Bacterial Species ◽  
Phenotypic Traits ◽  
Terminal Electron Acceptor ◽  
Content Type ◽  
Link Type ◽  
Natural Park ◽  
Fatty Acid Compositions

During the course of screening bacterial isolates as sources of as-yet unknown bioactive compounds with pharmaceutical applications, a chemo-organotrophic, Gram-negative bacterium was isolated from a soil sample taken from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain. Strain F-278,770T was oxidase- and catalase-positive, aerobic, with a respiratory type of metabolism with oxygen as the terminal electron acceptor, non-spore-forming and motile by one polar flagellum, although some cells had two polar flagella. Phylogenetic analysis of the 16S rRNA, gyrB, rpoB and rpoD genes revealed that strain F-278,770T belongs to the Pseudomonas koreensis subgroup (Pseudomonas fluorescens lineage), with Pseudomonas moraviensis , P. koreensis , P. baetica and P. helmanticensis as its closest relatives. Chemotaxonomic traits such as polar lipid and fatty acid compositions and G+C content of genomic DNA corroborated the placement of strain F-278,770T in the genus Pseudomonas . DNA–DNA hybridization assays and phenotypic traits confirmed that this strain represents a novel species of the genus Pseudomonas , for which the name Pseudomonas granadensis sp. nov. is proposed. The type strain is F-278,770T ( = DSM 28040T = LMG 27940T).

scholarly journals Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum

2005 ◽  
Vol 55 (3) ◽  
pp. 1305-1309 ◽  
Author(s):  
Raúl Rivas ◽  
Carmen Gutiérrez ◽  
Adriana Abril ◽  
Pedro F. Mateos ◽  
Eustoquio Martínez-Molina ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Gas Production ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences

Two sporulating bacterial strains designated CECAP06T and CECAP16 were isolated from the rhizosphere of the legume Cicer arietinum in Argentina. Almost-complete 16S rRNA gene sequences identified the isolates as a Paenibacillus species. It was most closely related to Paenibacillus cineris LMG 18439T (99·6 % sequence similarity), Paenibacillus favisporus LMG 20987T (99·4 % sequence similarity) and Paenibacillus azoreducens DSM 13822T (97·7 % sequence similarity). The cells of this novel species were motile, sporulating, rod-shaped, Gram-positive and strictly aerobic. The predominant fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The DNA G+C content of strains CECAP06T and CECAP16 was 51·3 and 50·9 mol%, respectively. Growth was observed from many carbohydrates, but gas production was not observed from glucose. Catalase and oxidase activities were present. The isolates produced β-galactosidase and hydrolysed aesculin. Gelatinase, caseinase and urease were not produced. The results of DNA–DNA hybridization showed that the strains from this study constitute a novel species of the genus Paenibacillus, for which the name Paenibacillus rhizosphaerae sp. nov. is proposed. The type strain is CECAP06T (=LMG 21955T=CECT 5831T).

Flavobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4289-4293 ◽  
Author(s):  
Yeon-Ju Kim ◽  
Sang-Rae Kim ◽  
Ngoc-Lan Nguyen ◽  
Deok-Chun Yang
Keyword(s):  
Novel Species ◽  
Strictly Aerobic ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
16S Rrna Sequence Analysis ◽  
Physiological And Biochemical ◽  
Motile By Gliding

A novel bacterial strain, designated DCY54T, was isolated from a field cultivated with ginseng in Yongin, Republic of Korea. Cells were Gram-reaction-negative, yellow-pigmented, rod-shaped, non-spore-forming, and strictly aerobic. They were motile by gliding and produced flexirubin-type pigments. Growth occurred optimally at 25–30 °C, at pH 5.0–7.0 and in the presence of 0–1 % NaCl. The 16S rRNA sequence analysis demonstrated that strain DCY54T was most closely related to Flavobacterium defluvii EMB117T (96.9 %). The only isoprenoid quinone of strain DCY 54T was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The major cellular fatty acids (>15 %) were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. The DNA G+C content was 33.3 mol%. Phylogenetic inference and phenotypic data supported affiliation of strain DCY54T to the genus Flavobacterium . Several physiological and biochemical tests differentiated strain DCY54T from the species of the genus Flavobacterium with validly published names. On the basis of data from a polyphasic study, strain DCY54T represents a novel species of the genus Flavobacterium for which the name Flavobacterium ginsengisoli sp. nov. is proposed. The type strain is DCY54T ( = KCTC 23318T = JCM 17336T).

scholarly journals Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species

2005 ◽  
Vol 55 (6) ◽  
pp. 2309-2315 ◽  
Author(s):  
Yuichi Nogi ◽  
Hideto Takami ◽  
Koki Horikoshi
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Hybridization ◽  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
16S Rrna Gene Sequencing ◽  
Industrial Applications ◽  
Rrna Gene ◽  
Bacterial Strains

Twenty alkaliphilic bacterial strains from industrial applications or enzyme studies were subjected to a polyphasic taxonomic investigation, including 16S rRNA gene sequencing, determination of genomic DNA G+C content, DNA–DNA hybridization, fatty acid analysis and standard bacteriological characterization. By comparing the groupings obtained based on the genomic DNA G+C content and the construction of a phylogenetic tree based on the 16S rRNA gene sequence, 12 clusters of similar strains were recognized. DNA–DNA hybridization revealed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus: Bacillus wakoensis sp. nov. (type strain N-1T=JCM 9140T=DSM 2521T), Bacillus hemicellulosilyticus sp. nov. (type strain C-11T=JCM 9152T=DSM 16731T), Bacillus cellulosilyticus sp. nov. (type strain N-4T=JCM 9156T=DSM 2522T), Bacillus akibai sp. nov. (type strain 1139T=JCM 9157T=ATCC 43226T) and Bacillus mannanilyticus sp. nov. (type strain AM-001T=JCM 10596T=DSM 16130T).

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