scholarly journals Genetic characterization of a novel picornavirus in turkeys (Meleagris gallopavo) distinct from turkey galliviruses and megriviruses and distantly related to the members of the genus Avihepatovirus

2013 ◽  
Vol 94 (7) ◽  
pp. 1496-1509 ◽  
Author(s):  
Ákos Boros ◽  
Csaba Nemes ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
Eric Delwart ◽  
...  
Keyword(s):  
Complete Genome ◽  
Hepatitis A Virus ◽  
Phylogenetic Analyses ◽  
Meleagris Gallopavo ◽  
Amino Acid Identity ◽  
Entry Site ◽  
Duck Hepatitis ◽  
Capsid Protein Vp1 ◽  
Vp1 Capsid Protein

This study reports the metagenomic detection and complete genome characterization of a novel turkey picornavirus from faecal samples of healthy (1/3) and affected (6/8) commercial turkeys with enteric and/or stunting syndrome in Hungary. The virus was detected at seven of the eight farms examined. The turkey/M176-TuASV/2011/HUN genome (KC465954) was genetically different from the currently known picornaviruses of turkey origin (megriviruses and galliviruses), and showed distant phylogenetic relationship and common genomic features (e.g. uncleaved VP0 and three predicted and unrelated 2A polypeptides) to duck hepatitis A virus (DHAV) of the genus Avihepatovirus. The complete genome analysis revealed multiple distinct genome features like the presence of two in-tandem aphthovirus 2A-like sequence repeats with DxExNPG/P ‘ribosome-skipping’ sites (76 %, 23/30 amino acids identical), with the first aphthovirus 2A-like sequence being located at the end of the VP1 capsid protein (VP1/2A1 ‘ribosome-skipping’ site). The phylogenetic analyses, low sequence identity (33, 32 and 36 % amino acid identity in P1, P2 and P3 regions) to DHAV, and the type II-like internal ribosome entry site suggests that this turkey picornavirus is related to, but distinct from the genus Avihepatovirus and it could be the founding member of a novel Avihepatovirus sister-clade genus. This is the third, taxonomically highly distinct picornavirus clade identified from turkeys exhibiting varied symptoms.

scholarly journals Genetic characterization of a novel duck-origin picornavirus with six 2A proteins

2014 ◽  
Vol 95 (6) ◽  
pp. 1289-1296 ◽  
Author(s):  
Xiaoyan Wang ◽  
Ning Liu ◽  
Fumin Wang ◽  
Kang Ning ◽  
Yanbo Li ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Genetic Characterization ◽  
Hepatitis A Virus ◽  
Phylogenetic Analyses ◽  
Entry Site ◽  
Internal Ribosome Entry ◽  
Founding Member ◽  
Duck Hepatitis ◽  
Novel Virus

A novel virus was detected from diseased ducks and completely determined. The virus was shown to have a picornavirus-like genome layout. Interestingly, the genome contained a total of up to six 2As, including four 2As (2A1–2A4) each having an NPGP motif, an AIG1-like 2A5, and a parechovirus-like 2A6. The 5′UTR was predicted to possess a hepacivirus/pestivirus-like internal ribosome entry site (IRES). However, the subdomain IIIe consisted of a 3 nt stem and five unpaired bases, distinct from those found in all other HP-like IRESs. The virus was most closely related to duck hepatitis A virus, with amino acid identities of 37.7 %, 39 % and 43.7 % in the P1, P2 and P3 regions, respectively. Based on these investigations, together with phylogenetic analyses, the virus could be considered as the founding member of a novel picornavirus genus that we tentatively named ‘Aalivirus’, with ‘Aalivirus A’ as the type species.

scholarly journals Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam

2016 ◽  
Vol 10 (09) ◽  
pp. 988-995 ◽  
Author(s):  
Huong Thi Thanh Doan ◽  
Xuyen Thi Kim Le ◽  
Roan Thi Do ◽  
Chau Thi Minh Hoang ◽  
Khue Thi Nguyen ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Phylogenetic Analyses ◽  
Hypervariable Region ◽  
Genetic Characteristics ◽  
Molecular Genotyping ◽  
Duck Hepatitis ◽  
Polymerase Chain ◽  
Different Strains

Introduction: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during 2009–2013. Methodology: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified by polymerase chain reaction (PCR). Then, VP1 genes of eleven positive samples and two attenuated vaccine strains were sequenced and analyzed. Results: Genotypic and phylogenetic analyses indicated that the 13 Vietnamese isolates were classified into two genotypes, DHAV-1 and DHAV-3. The rate of identity and homology was 91%–100% between the 10 Vietnamese and 26 global strains of DHAV-3, and 92%–100% between 3 Vietnamese and 16 strains of DHAV-1. Between the DHAV-3 and DHAV-1 strains, the divergence reached 30%. At the C-terminal of VP1 for the different strains, a hypervariable region was observed, and notably, six of the Vietnamese DHAV-3 strains in this study showed four consistent differences (at positions T184M, Q200H, K207N, and K214R) within this group that were distinct from all other DHAV-3 strains. Conclusions: This is the first report of molecular characterization of DHAVs in Vietnam. At least two genotypes were identified, DHAV-1 and DHAV-3, with diversified clades within and between genotypes. DHAV-3 seemed to be dominant in Vietnam.

scholarly journals Identification and complete genome characterization of a novel picornavirus in turkey (Meleagris gallopavo)

2012 ◽  
Vol 93 (10) ◽  
pp. 2171-2182 ◽  
Author(s):  
Ákos Boros ◽  
Csaba Nemes ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
Eric Delwart ◽  
...  
Keyword(s):  
Amino Acid ◽  
Complete Genome ◽  
Bird Species ◽  
Entry Site ◽  
Pathogenic Potential ◽  
Genome Characterization ◽  
Genome Region ◽  
Faecal Samples ◽  
The Usa

Members of the family Picornaviridae are important pathogens of humans and animals, although compared with the thousands of known bird species (>10 000), only a few (n = 11) picornaviruses have been identified from avian sources. This study reports the metagenomic detection and complete genome characterization of a novel turkey picornavirus from faecal samples collected from eight turkey farms in Hungary. Using RT-PCR, both healthy (two of three) and affected (seven of eight) commercial turkeys with enteric and/or stunting syndrome were shown to be shedding viruses in seven (88 %) of the eight farms. The viral genome sequence (turkey/M176/2011/HUN; GenBank accession no. JQ691613) shows a high degree of amino acid sequence identity (96 %) to the partial P3 genome region of a picornavirus reported recently in turkey and chickens from the USA and probably belongs to the same species. In the P1 and P2 regions, turkey/M176/2011/HUN is related most closely to, but distinct from, the kobuviruses and turdivirus 1. Complete genome analysis revealed the presence of characteristic picornaviral amino acid motifs, a potential type II-like 5′ UTR internal ribosome entry site (first identified among avian-origin picornaviruses) and a conserved, 48 nt long ‘barbell-like’ structure found at the 3′ UTR of turkey/M176/2011/HUN and members of the picornavirus genera Avihepatovirus and Kobuvirus. The general presence of turkey picornavirus – a novel picornavirus species – in faecal samples from healthy and affected turkeys in Hungary and in the USA suggests the worldwide occurrence and endemic circulation of this virus in turkey farms. Further studies are needed to investigate the aetiological role and pathogenic potential of this picornavirus in food animals.

scholarly journals Complete Genome Sequence of a Duck Hepatitis A Virus Type 3 Identified in Eastern China

2012 ◽  
Vol 86 (24) ◽  
pp. 13848-13848 ◽  
Author(s):  
Q. Xu ◽  
R. Zhang ◽  
L. Chen ◽  
L. Yang ◽  
J. Li ◽  
...  
Keyword(s):  
Virus Type ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Eastern China ◽  
Duck Hepatitis ◽  
Type 3

Isolation and characterization of a low pathogenic duck hepatitis A virus 3 from South Korea

2013 ◽  
Vol 162 (1) ◽  
pp. 254-258 ◽  
Author(s):  
Se-Yeoun Cha ◽  
Jae-Hee Roh ◽  
Min Kang ◽  
Bumseok Kim ◽  
Hyung-Kwan Jang
Keyword(s):  
South Korea ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Isolation And Characterization ◽  
Duck Hepatitis

scholarly journals Maturation of the Hepatitis A Virus Capsid Protein VP1 Is Not Dependent on Processing by the 3CproProteinase

1999 ◽  
Vol 73 (8) ◽  
pp. 6220-6227 ◽  
Author(s):  
Annette Martin ◽  
Danièle Bénichou ◽  
Shih-Fong Chao ◽  
Lisette M. Cohen ◽  
Stanley M. Lemon
Keyword(s):  
Capsid Protein ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Expression System ◽  
Site Directed Mutagenesis ◽  
Recognition Sequence ◽  
C Terminus ◽  
Vaccinia Viruses ◽  
Capsid Protein Vp1 ◽  
Vp1 Capsid Protein

ABSTRACT Most details of the processing of the hepatitis A virus (HAV) polyprotein are known. Unique among members of the familyPicornaviridae, the primary cleavage of the HAV polyprotein is mediated by 3Cpro, the only proteinase known to be encoded by the virus, at the 2A/2B junction. All other cleavages of the polyprotein have been considered to be due to 3Cpro, although the precise location and mechanism responsible for the VP1/2A cleavage have been controversial. Here we present data that argue strongly against the involvement of the HAV 3Cproproteinase in the maturation of VP1 from its VP1-2A precursor. Using a heterologous expression system based on recombinant vaccinia viruses directing the expression of full-length or truncated capsid protein precursors, we show that the C terminus of the mature VP1 capsid protein is located near residue 764 of the polyprotein. However, a proteolytically active HAV 3Cpro that was capable of directing both VP0/VP3 and VP3/VP1 cleavages in vaccinia virus-infected cells failed to process the VP1-2A precursor. Using site-directed mutagenesis of an infectious molecular clone of HAV, we modified potential VP1/2A cleavage sites that fit known 3Cprorecognition criteria and found that a substitution that ablates the presumed 3Cpro dipeptide recognition sequence at Glu764-Ser765 abolished neither infectivity nor normal VP1 maturation. Altered electrophoretic mobility of VP1 from a viable mutant virus with an Arg764 substitution indicated that this residue is present in VP1 and that the VP1/2A cleavage occurs downstream of this residue. These data indicate that maturation of the HAV VP1 capsid protein is not dependent on 3Cpro processing and may thus be uniquely dependent on a cellular proteinase.

scholarly journals Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1

2018 ◽  
Vol 9 ◽  
Author(s):  
Jinyan Xie ◽  
Qiurui Zeng ◽  
Mingshu Wang ◽  
Xumin Ou ◽  
Yunchao Ma ◽  
...  
Keyword(s):  
Virus Type ◽  
Chicken Embryo ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Duck Hepatitis

Sequence Analysis and B Cell Epitope Prediction of Duck Hepatitis A Virus 1 VP1 Gene

2013 ◽  
Vol 647 ◽  
pp. 214-219
Author(s):  
Xing Jian Wen ◽  
An Chun Cheng ◽  
Ming Shu Wang
Keyword(s):  
B Cell ◽  
Hepatitis A Virus ◽  
Phylogenetic Analyses ◽  
Cell Epitope ◽  
Epitope Prediction ◽  
Alpha Helix ◽  
Vp1 Gene ◽  
Vp1 Protein ◽  
Duck Hepatitis ◽  
B Cell Epitope

Molecular characterization and phylogenetic analysis of the DHAV-1 VP1 gene, structure and biological function of the VP1 protein were analyzed by using the programs of the DNAstar software ,MEGA software and other bioinformatics software on the line. The nucleotide sequence homologies among and the amino acids homologies of the VP1 gene of DHAV H stain with other 13 DHAV-1 strains in the GenBank were 92.54%-99.86% and 93.75%-99.57%.The putative secondary structure of the VP1 protein contained 10.08% H(alpha helix), 38.24% E(beta-sheet), 51.68%L(loop/coil), Protein can be classified as mixed. The results showed that the B cell epitopes of the VP1 protein were located at the C-terminal 131-136, and 209-218 regions according to Kyte-Doolitte method, Emini method,Karlus-Schulz method, and Jameson-Wolf method implemented in Protean program of DNAStar 7.The VP1 protein in the surface of the virus most exposed, it is to decide the major components of the effects of the virus of Picornaviridae. The VP1 protein of DHAV is a good biomaterial for vaccine research, and genetic and phylogenetic analyses of the VP1 and the B cell epitope prediction can help us to develop multi-epitope vaccine against DHAV variant viruses infections.

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