scholarly journals The role of coherence in bacterial communication

2017 ◽  
Author(s):  
Sarangam Majumdar ◽  
Sisir Roy
Keyword(s):  
Quorum Sensing ◽  
Ion Channel ◽  
Communication Systems ◽  
Phase Synchronization ◽  
Cell Communication ◽  
Communication Process ◽  
Bacterial Communication ◽  
Novel Approach ◽  
Electrical Signaling

Bacteria within biofilms can coordinate their behavior through distinct from of communication mechanism1. The well-established cell - to - cell signaling process in bacteria is known as quorum sensing through chemical signaling molecules2-5. Recently, another cell- to - cell communication process based on ion channel mediated electrical signaling6 has also been observed. In this article, we propose a novel approach to explain the role of coherence and phase synchronization in the cell – to – cell bacterial communication. The observable long – range coherent electrical signaling is species independent and it is caused by membrane – potential - dependent modulation of tumbling frequency7-9. Moreover, noise can play a constructive role in enhancing the synchronization of chaotic bacterial communication systems and noise associated with the opening and closing the gate of ion channel induce small kinetic viscosity that make a wave-like pattern in concentration profile of quorum sensing.

scholarly journals Quorum Sensing in the Context of Food Microbiology

2012 ◽  
Vol 78 (16) ◽  
pp. 5473-5482 ◽  
Author(s):  
Panagiotis N. Skandamis ◽  
George-John E. Nychas
Keyword(s):  
Food Safety ◽  
Quorum Sensing ◽  
Communication Systems ◽  
Cell Communication ◽  
Food Preservation ◽  
Food Microbiology ◽  
Limited Information ◽  
Food Spoilage ◽  
The Many

ABSTRACTFood spoilage may be defined as a process that renders a product undesirable or unacceptable for consumption and is the outcome of the biochemical activity of a microbial community that eventually dominates according to the prevailing ecological determinants. Although limited information are reported, this activity has been attributed to quorum sensing (QS). Consequently, the potential role of cell-to-cell communication in food spoilage and food safety should be more extensively elucidated. Such information would be helpful in designing approaches for manipulating these communication systems, thereby reducing or preventing, for instance, spoilage reactions or even controlling the expression of virulence factors. Due to the many reports in the literature on the fundamental features of QS, e.g., chemistry and definitions of QS compounds, in this minireview, we only allude to the types and chemistry of QS signaling moleculesper seand to the (bioassay-based) methods of their detection and quantification, avoiding extensive documentation. Conversely, we attempt to provide insights into (i) the role of QS in food spoilage, (ii) the factors that may quench the activity of QS in foods and review the potential QS inhibitors that might “mislead” the bacterial coordination of spoilage activities and thus may be used as biopreservatives, and (iii) the future experimental approaches that need to be undertaken in order to explore the “gray” or “black” areas of QS, increase our understanding of how QS affects microbial behavior in foods, and assist in finding answers as to how we can exploit QS for the benefit of food preservation and food safety.

scholarly journals Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Betty L. Slinger ◽  
Jacqueline J. Deay ◽  
Josephine R. Chandler ◽  
Helen E. Blackwell
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Homoserine Lactone ◽  
Communication Process ◽  
Burkholderia Cepacia Complex ◽  
Infection Model ◽  
Bacterial Communication ◽  
Human Infections ◽  
Chemical Strategies

Abstract The Burkholderia cepacia complex (Bcc) is a family of closely related bacterial pathogens that are the causative agent of deadly human infections. Virulence in Bcc species has been shown to be controlled by the CepI/CepR quorum sensing (QS) system, which is mediated by an N-acyl L-homoserine lactone (AHL) signal (C8-AHL) and its cognate LuxR-type receptor (CepR). Chemical strategies to block QS in Bcc members would represent an approach to intercept this bacterial communication process and further delineate its role in infection. In the current study, we sought to identify non-native AHLs capable of agonizing or antagonizing CepR, and thereby QS, in a Bcc member. We screened a library of AHL analogs in cell-based reporters for CepR, and identified numerous highly potent CepR agonists and antagonists. These compounds remain active in a Bcc member, B. multivorans, with one agonist 250-fold more potent than the native ligand C8-AHL, and can affect QS-controlled motility. Further, the CepR antagonists prolong C. elegans survival in an infection model. These AHL analogs are the first reported non-native molecules that both directly modulate CepR and impact QS-controlled phenotypes in a Bcc member, and represent valuable chemical tools to assess the role of QS in Bcc infections.

Growth of Salmonella Enteritidis in the presence of quorum sensing signaling compounds produced by Pseudomonas aeruginosa

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Weijia He ◽  
Huamei Yang ◽  
Xiang Wang ◽  
Hongmei Li ◽  
Qingli Dong
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Salmonella Enteritidis ◽  
Culture Supernatant ◽  
Lag Time ◽  
Bacterial Communication ◽  
Homoserine Lactones ◽  
Positive Effects ◽  
Kinetics Of

Abstract Quorum sensing (QS) can exist in food-related bacteria and potentially affect bacterial growth through acyl-homoserine lactones (AHLs). To verify the role of QS compounds in the cell-free supernatant, this study examined the effect of supernatant extracted from Pseudomonas aeruginosa culture on the growth kinetics of Salmonella Enteritidis. The results showed that the lag time (λ) of S. Enteritidis was apparently reduced (p < 0.05) under the influence of P. aeruginosa culture supernatant compared with the S. Enteritidis culture supernatant. HPLC-MS/MS test demonstrated that AHLs secreted by P. aeruginosa were mainly C14-HSL with a content of 85.71 μg/mL and a small amount of 3-oxo-C12-HSL. In addition, the commercially synthetic C14-HSL had positive effects on the growth of S. Enteritidis, confirming once again that the growth of S. Enteritidis was affected by AHL metabolized by other bacteria and the complexity of bacterial communication.

Close-up on a bacterial informational war in the geocaulosphere

2020 ◽  
Vol 66 (7) ◽  
pp. 447-454 ◽  
Author(s):  
Andrea Chane ◽  
Yvann Bourigault ◽  
Mathilde Bouteiller ◽  
Yoan Konto-Ghiorghi ◽  
Annabelle Merieau ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Communication Systems ◽  
Cell Communication ◽  
Soft Rot ◽  
Homoserine Lactone ◽  
Signaling Molecules ◽  
Disease Symptoms ◽  
Reporter Strains ◽  
Bright Green ◽  
Bright Green Fluorescence

The geocaulosphere is home to microbes that establish communication between themselves and others that disrupt them. These cell-to-cell communication systems are based on the synthesis and perception of signaling molecules, of which the best known belong to the N-acyl-homoserine lactone (AHL) family. Among indigenous bacteria, certain Gram-positive actinobacteria can sense AHLs produced by soft-rot Gram-negative phytopathogens and can degrade the quorum-sensing AHL signals to impair the expression of virulence factors. We mimicked this interaction by introducing dual-color reporter strains suitable for monitoring both the location of the cells and their quorum-sensing and -quenching activities, in potato tubers. The exchange of AHL signals within the pathogen’s cell quorum was clearly detected by the presence of bright green fluorescence instead of blue in a portion of Pectobacterium-tagged cells. This phenomenon in Rhodococcus cells was accompanied by a change from red fluorescence to orange, showing that the disappearance of signaling molecules is due to rhodococcal AHL degradation rather than the inhibition of AHL production. Rhodococci are victorious in this fight for the control of AHL-based communication, as their jamming activity is powerful enough to prevent the onset of disease symptoms.

scholarly journals Quorum sensing controls thePseudomonas aeruginosaCRISPR-Cas adaptive immune system

2016 ◽  
Vol 114 (1) ◽  
pp. 131-135 ◽  
Author(s):  
Nina M. Høyland-Kroghsbo ◽  
Jon Paczkowski ◽  
Sampriti Mukherjee ◽  
Jenny Broniewski ◽  
Edze Westra ◽  
...  
Keyword(s):  
Immune System ◽  
Quorum Sensing ◽  
Cell Communication ◽  
Adaptive Immune System ◽  
Communication Process ◽  
Clinical Settings ◽  
Bacterial Populations ◽  
Adaptive Immune ◽  
Quorum Sensing Inhibitors ◽  
Adaptive Immune Systems

CRISPR-Cas are prokaryotic adaptive immune systems that provide protection against bacteriophage (phage) and other parasites. Little is known about how CRISPR-Cas systems are regulated, preventing prediction of phage dynamics in nature and manipulation of phage resistance in clinical settings. Here, we show that the bacteriumPseudomonas aeruginosaPA14 uses the cell–cell communication process, called quorum sensing, to activatecasgene expression, to increase CRISPR-Cas targeting of foreign DNA, and to promote CRISPR adaptation, all at high cell density. This regulatory mechanism ensures maximum CRISPR-Cas function when bacterial populations are at highest risk for phage infection. We demonstrate that CRISPR-Cas activity and acquisition of resistance can be modulated by administration of pro- and antiquorum-sensing compounds. We propose that quorum-sensing inhibitors could be used to suppress the CRISPR-Cas adaptive immune system to enhance medical applications, including phage therapies.

scholarly journals The PqsE-RhlR Interaction Regulates RhlR DNA Binding to Control Virulence Factor Production in Pseudomonas aeruginosa

2022 ◽  
Author(s):  
Kayla A. Simanek ◽  
Isabelle R. Taylor ◽  
Erica K. Richael ◽  
Erica Lasek-Nesselquist ◽  
Bonnie L. Bassler ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Dna Binding ◽  
Quorum Sensing ◽  
Virulence Factor ◽  
Cell Communication ◽  
Communication Process ◽  
Collective Behaviors ◽  
Factor Production ◽  
A Cell ◽  
Virulence Factor Production

Bacteria use a cell-cell communication process called quorum sensing (QS) to orchestrate collective behaviors. QS relies on the group-wide detection of molecules called autoinducers (AI).

scholarly journals Legionellashows a diverse secondary metabolism dependent on a broad spectrum Sfp-type phosphopantetheinyl transferase

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2720 ◽  
Author(s):  
Nicholas J. Tobias ◽  
Tilman Ahrendt ◽  
Ursula Schell ◽  
Melissa Miltenberger ◽  
Hubert Hilbi ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Communication Systems ◽  
Fatty Acid Biosynthesis ◽  
Cell Communication ◽  
Gene Clusters ◽  
Phosphopantetheinyl Transferase ◽  
Biosynthetic Gene Clusters ◽  
Legionnaires Disease ◽  
Huge Variety

Several members of the genusLegionellacause Legionnaires’ disease, a potentially debilitating form of pneumonia. Studies frequently focus on the abundant number of virulence factors present in this genus. However, what is often overlooked is the role of secondary metabolites fromLegionella. Following whole genome sequencing, we assembled and annotated theLegionella parisiensisDSM 19216 genome. Together with 14 other members of theLegionella, we performed comparative genomics and analysed the secondary metabolite potential of each strain. We found thatLegionellacontains a huge variety of biosynthetic gene clusters (BGCs) that are potentially making a significant number of novel natural products with undefined function. Surprisingly, only a single Sfp-like phosphopantetheinyl transferase is found in allLegionellastrains analyzed that might be responsible for the activation of all carrier proteins in primary (fatty acid biosynthesis) and secondary metabolism (polyketide and non-ribosomal peptide synthesis). Using conserved active site motifs, we predict some novel compounds that are probably involved in cell-cell communication, differing to known communication systems. We identify several gene clusters, which may represent novel signaling mechanisms and demonstrate the natural product potential ofLegionella.

scholarly journals Saccharomyces cerevisiae Requires CFF1 To Produce 4-Hydroxy-5-Methylfuran-3(2H)-One, a Mimic of the Bacterial Quorum-Sensing Autoinducer AI-2

mBio ◽  
2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Julie S. Valastyan ◽  
Christina M. Kraml ◽  
Istvan Pelczer ◽  
Thomas Ferrante ◽  
Bonnie L. Bassler
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Quorum Sensing ◽  
Bacterial Species ◽  
Cell Communication ◽  
Communication Process ◽  
Signal Molecules ◽  
Catalytic Residue ◽  
Interspecies Communication ◽  
Content Type ◽  
Extracellular Signal

ABSTRACT Quorum sensing is a process of cell-to-cell communication that bacteria use to orchestrate collective behaviors. Quorum sensing depends on the production, release, and detection of extracellular signal molecules called autoinducers (AIs) that accumulate with increasing cell density. While most AIs are species specific, the AI called AI-2 is produced and detected by diverse bacterial species, and it mediates interspecies communication. We recently reported that mammalian cells produce an AI-2 mimic that can be detected by bacteria through the AI-2 receptor LuxP, potentially expanding the role of the AI-2 system to interdomain communication. Here, we describe a second molecule capable of interdomain signaling through LuxP, 4-hydroxy-5-methylfuran-3(2H)-one (MHF), that is produced by the yeast Saccharomyces cerevisiae. Screening the S. cerevisiae deletion collection revealed Cff1p, a protein with no known role, to be required for MHF production. Cff1p is proposed to be an enzyme, with structural similarity to sugar isomerases and epimerases, and substitution at the putative catalytic residue eliminated MHF production in S. cerevisiae. Sequence analysis uncovered Cff1p homologs in many species, primarily bacterial and fungal, but also viral, archaeal, and higher eukaryotic. Cff1p homologs from organisms from all domains can complement a cff1Δ S. cerevisiae mutant and restore MHF production. In all cases tested, the identified catalytic residue is conserved and required for MHF to be produced. These findings increase the scope of possibilities for interdomain interactions via AI-2 and AI-2 mimics, highlighting the breadth of molecules and organisms that could participate in quorum sensing. IMPORTANCE Quorum sensing is a cell-to-cell communication process that bacteria use to monitor local population density. Quorum sensing relies on extracellular signal molecules called autoinducers (AIs). One AI called AI-2 is broadly made by bacteria and used for interspecies communication. Here, we describe a eukaryotic AI-2 mimic, 4-hydroxy-5-methylfuran-3(2H)-one, (MHF), that is made by the yeast Saccharomyces cerevisiae, and we identify the Cff1p protein as essential for MHF production. Hundreds of viral, archaeal, bacterial, and eukaryotic organisms possess Cff1p homologs. This finding, combined with our results showing that homologs from all domains can replace S. cerevisiae Cff1p, suggests that like AI-2, MHF is widely produced. Our results expand the breadth of organisms that may participate in quorum-sensing-mediated interactions.

scholarly journals Effects of Natural Products on Bacterial Communication and Network-Quorum Sensing

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Min Yang ◽  
Fanying Meng ◽  
Wen Gu ◽  
Fengjiao Li ◽  
Yating Tao ◽  
...  
Keyword(s):  
Natural Products ◽  
Quorum Sensing ◽  
Virulence Factor ◽  
Biofilm Formation ◽  
Bacterial Flora ◽  
External Environment ◽  
Cell Communication ◽  
Beneficial Bacteria ◽  
Bacterial Communication ◽  
Virulence Factor Production

Quorum sensing (QS) has emerged as a research hotspot in microbiology and medicine. QS is a regulatory cell communication system used by bacterial flora to signal to the external environment. QS influences bacterial growth, proliferation, biofilm formation, virulence factor production, antibiotic synthesis, and environmental adaptation. Through the QS system, natural products can regulate the growth of harmful bacteria and enhance the growth of beneficial bacteria, thereby improving human health. Herein, we review advances in the discovery of natural products that regulate bacterial QS systems.

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