scholarly journals Tirant stealthily invaded natural Drosophila melanogaster populations during the last century

2020 ◽  
Author(s):  
Florian Schwarz ◽  
Filip Wierzbicki ◽  
Kirsten-André Senti ◽  
Robert Kofler
Keyword(s):  
Drosophila Melanogaster ◽  
Insertion Site ◽  
Natural Populations ◽  
P Element ◽  
Past Century ◽  
Time Points ◽  
The Past ◽  
Living Fossils ◽  
Late Discovery ◽  
Comprehensive Study

AbstractIt was long thought that solely three different transposable elements - the I-element, the P-element and hobo - invaded natural D. melanogaster populations within the last century. By sequencing the ‘living fossils’ of Drosophila research, i.e. D. melanogaster strains sampled from natural populations at different time points, we show that a fourth TE, Tirant, invaded D. melanogaster populations during the past century. Tirant likely spread in D. melanogaster populations around 1938, followed by the I-element, hobo, and, lastly, the P-element. In addition to the recent insertions of the canonical Tirant, D. melanogaster strains harbour degraded Tirant sequences in the heterochromatin which are likely due to an ancient invasion, possibly predating the split of D. melanogaster and D. simulans. In contrast to the I-element, P-element and hobo, we did not find that Tirant induces any hybrid dysgenesis symptoms. This absence of apparent phenotypic effects may explain the late discovery of the Tirant invasion. Recent Tirant insertions were found in all investigated natural populations. Populations from Tasmania carry distinct Tirant sequences, likely due to a founder effect. By investigating the TE composition of natural populations and strains sampled at different time points, insertion site polymorphisms, piRNAs and phenotypic effects, we provide a comprehensive study of a natural TE invasion.

scholarly journals Tirant Stealthily Invaded Natural Drosophila melanogaster Populations during the Last Century

2020 ◽  
Author(s):  
Florian Schwarz ◽  
Filip Wierzbicki ◽  
Kirsten-André Senti ◽  
Robert Kofler
Keyword(s):  
Drosophila Melanogaster ◽  
Insertion Site ◽  
Natural Populations ◽  
P Element ◽  
Past Century ◽  
The Novel ◽  
Time Points ◽  
Living Fossils ◽  
Drosophila Research ◽  
Late Discovery

Abstract It was long thought that solely three different transposable elements (TEs)—the I-element, the P-element, and hobo—invaded natural Drosophila melanogaster populations within the last century. By sequencing the “living fossils” of Drosophila research, that is, D. melanogaster strains sampled from natural populations at different time points, we show that a fourth TE, Tirant, invaded D. melanogaster populations during the past century. Tirant likely spread in D. melanogaster populations around 1938, followed by the I-element, hobo, and, lastly, the P-element. In addition to the recent insertions of the canonical Tirant, D. melanogaster strains harbor degraded Tirant sequences in the heterochromatin which are likely due to an ancient invasion, likely predating the split of D. melanogaster and D. simulans. These degraded insertions produce distinct piRNAs that were unable to prevent the novel Tirant invasion. In contrast to the I-element, P-element, and hobo, we did not find that Tirant induces any hybrid dysgenesis symptoms. This absence of apparent phenotypic effects may explain the late discovery of the Tirant invasion. Recent Tirant insertions were found in all investigated natural populations. Populations from Tasmania carry distinct Tirant sequences, likely due to a founder effect. By investigating the TE composition of natural populations and strains sampled at different time points, insertion site polymorphisms, piRNAs, and phenotypic effects, we provide a comprehensive study of a natural TE invasion.

scholarly journals The Regulatory Properties of Autonomous Subtelomeric P Elements Are Sensitive to a Suppressor of variegation in Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 143 (4) ◽  
pp. 1663-1674 ◽  
Author(s):  
Stéphane Ronsseray ◽  
Monique Lehmann ◽  
Danielle Nouaud ◽  
Dominique Anxolabéhère
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Recombination ◽  
Natural Populations ◽  
P Element ◽  
Single Element ◽  
Heterochromatin Protein 1 ◽  
X Chromosomes ◽  
P Elements ◽  
Associated Sequences ◽  
Regulatory Properties

Abstract Genetic recombination was used in Drosophila melanogaster to isolate P elements, inserted at the telomeres of X chromosomes (cytological site 1A) from natural populations, in a genetic background devoid of other P elements. We show that complete maternally inherited P repression in the germline (P cytotype) can be elicited by only two autonomous P elements at 1A and that a single element at this site has partial regulatory properties. The analysis of the surrounding chromosomal regions of the P elements at 1A shows that in all cases these elements are flanked by Telomeric Associated Sequences, tandemly repetitive noncoding sequences that have properties of heterochromatin. In addition, we show that the regulatory properties of P elements at 1A can be inhibited by some of the mutant alleles of the Su(var)205 gene and by a deficiency of this gene. However, the regulatory properties of reference P strains (Harwich and Texas 007) are not impaired by Su(var)205 mutations. Su(var)205 encodes Heterochromatin Protein 1 (HP1). These results suggest that the HP1 dosage effect on the P element properties is sitedependent and could involve the structure of the chromatin.

Molecular organization and expression of the genetic locus glued in Drosophila melanogaster

1986 ◽  
Vol 6 (3) ◽  
pp. 833-841
Author(s):  
A Swaroop ◽  
J W Sun ◽  
M L Paco-Larson ◽  
A Garen
Keyword(s):  
Drosophila Melanogaster ◽  
Cell Function ◽  
Genetic Locus ◽  
Insertion Site ◽  
Lethal Effect ◽  
P Element ◽  
Molecular Organization ◽  
General Distribution ◽  
Element Insertion ◽  
Mutant Phenotypes

The Glued locus of Drosophila melanogaster is genetically defined as the functional unit which is affected by the dominant Glued mutation Gl. Genomic DNA was cloned from the region of the Glued locus, at 70C2 on chromosome 3, by using a P element insertion in the region as a molecular marker. Three genes encoding polyadenylated transcripts were detected within a 30-kilobase span of the cloned DNA. The gene nearest the P element insertion site was identified as a Glued gene on the basis of alterations in its DNA and encoded transcript associated with the Gl mutation and with reversions of Gl which eliminate the dominant effect by inactivation of the mutant allele. Expression of the wild-type Gl+ gene is temporally regulated during development; the amount of the encoded transcript is highest in the embryonic stage, decreasing in the first and second larval instars, and then increasing in the third instar and pupal stages. There is a maternal contribution of the Gl+ transcript to the embryo, which probably accounts for the maternal lethal effect of Glued mutations on early development. In situ hybridizations of Gl+ DNA to RNA in tissue sections showed that the Gl+ transcript is present in virtually all tissues of the embryo, late larva, and pupa. The general distribution of this transcript is consistent with genetic evidence indicating that the Glued locus controls a generally essential cell function (P. J. Harte and D. R. Kankel, Genetics 101:477-501, 1982). Different Glued mutations produce distinct phenotypic effects, including adults with severe visual defects, larvae lacking imaginal discs, and early lethality. These diverse mutant phenotypes are discussed in terms of quantitative changes in the Glued function. Closely adjacent to Gl+ is another gene which is transcribed in a divergent direction and expressed coordinately with Gl+ throughout Drosophila development. It remains to be determined whether this gene is also involved with the Glued function.

scholarly journals A scenario for the hobo transposable element invasion, deduced from the structure of natural populations of Drosophila melanogaster using tandem TPE repeats

2000 ◽  
Vol 75 (1) ◽  
pp. 13-23 ◽  
Author(s):  
ERIC BONNIVARD ◽  
CLAUDE BAZIN ◽  
BEATRICE DENIS ◽  
DOMINIQUE HIGUET
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Markers ◽  
World Wide ◽  
Tandem Repeats ◽  
Natural Populations ◽  
The Past ◽  
The World ◽  
Historical Pattern ◽  
The 1960S ◽  
Two Stages

Temporal surveys of hobo transposable elements in natural populations reveal a historical pattern suggesting a recent world-wide invasion of D. melanogaster by these transposons, perhaps following a recent horizontal transfer. To clarify the dynamics of hobo elements in natural populations, and thus to provide further data for our understanding of the hobo invasion, TPE tandem repeats, observed in the polymorphic S region of the element, were used as molecular markers. The number of TPE repeats was studied in 101 current populations from around the world, and in 63 strains collected in the past. This revealed a geographical distribution which seems to have been stable since the beginning of the 1960s. This distribution is compatible with a number of hypotheses for the dynamics of hobo elements. We propose a scenario based on an invasion in two stages: first, a complete invasion by elements with three TPE repeats, followed by the beginning of a new invasion involving hobo elements with five or seven repeats.

Genetic maps of the proximal half of chromosome arm 2L of Drosophila melanogaster

Genome ◽  
2007 ◽  
Vol 50 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Sylvia Glen Levine ◽  
Suchot Sunday ◽  
Ruth E. Dörig ◽  
Beat Suter ◽  
Paul Lasko
Keyword(s):  
Drosophila Melanogaster ◽  
Large Scale ◽  
Research Community ◽  
Genetic Maps ◽  
Past Century ◽  
The Past ◽  
Proximal Half ◽  
Chromosome Arm ◽  
Physiologic Function ◽  
Complementation Groups

Drosophila mutants have played an important role in elucidating the physiologic function of genes. Large-scale projects have succeeded in producing mutations in a large proportion of Drosophila genes. Many mutant fly lines have also been produced through the efforts of individual laboratories over the past century. In an effort to make some of these mutants more useful to the research community, we systematically mapped a large number of mutations affecting genes in the proximal half of chromosome arm 2L to more precisely defined regions, defined by deficiency intervals, and, when possible, by individual complementation groups. To further analyze regions 36 and 39–40, we produced 11 new deficiencies with gamma irradiation, and we constructed 6 new deficiencies in region 30–33, using the DrosDel system. trans-heterozygous combinations of deficiencies revealed 5 additional functions, essential for viability or fertility.

scholarly journals Molecular cloning of suppressor of sable, a Drosophila melanogaster transposon-mediated suppressor.

1986 ◽  
Vol 6 (5) ◽  
pp. 1520-1528 ◽  
Author(s):  
D Y Chang ◽  
B Wisely ◽  
S M Huang ◽  
R A Voelker
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Insertion Site ◽  
Hybrid Dysgenesis ◽  
P Element ◽  
Dna Transformation ◽  
Wild Type ◽  
Induced Mutations ◽  
X Ray ◽  
Element Insertion

A hybrid dysgenesis-induced allele [su(s)w20] associated with a P-element insertion was used to clone sequences from the su(s) region of Drosophila melanogaster by means of the transposon-tagging technique. Cloned sequences were used to probe restriction enzyme-digested DNAs from 22 other su(s) mutations. None of three X-ray-induced or six ethyl methanesulfonate-induced su(s) mutations possessed detectable variation. Seven spontaneous, four hybrid dysgenesis-induced, and two DNA transformation-induced mutations were associated with insertions within 2.0 kilobases (kb) of the su(s)w20 P-element insertion site. When the region of DNA that included the mutational insertions was used to probe poly(A)+ RNAs, a 5-kb message was detected in wild-type RNA that was present in greatly reduced amounts in two su(s) mutations. By using strand-specific probes, the direction of transcription of the 5-kb message was determined. The mutational insertions lie in DNA sequences near the 5' end of the 5-kb message. Three of the seven spontaneous su(s) mutations are associated with gypsy insertions, but they are not suppressible by su(Hw).

scholarly journals Accumulation of P elements in minority inversions in natural populations of Drosophila melanogaster

1992 ◽  
Vol 59 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Walter F. Eanes ◽  
Cedric Wesley ◽  
Brian Charlesworth
Keyword(s):  
Mathematical Model ◽  
Drosophila Melanogaster ◽  
Copy Number ◽  
Natural Populations ◽  
P Element ◽  
P Elements ◽  
Chromosomal Inversions ◽  
Associated Production ◽  
African Populations ◽  
Parameter Values

SummaryThe accumulation of a transposable element inside chromosomal inversions is examined theoretically by a mathematical model, and empirically by counts of P elements associated with inversion polymorphisms in natural populations of Drosophila melanogaster. The model demonstrates that, if heterozygosity for an inversion effectively reduces element associated production of detrimental chromosome rearrangements, a differential accumulation of elements is expected, with increased copy number inside the minority inversion. Several-fold differential accumulations are possible with certain parameter values. We present data on P element counts for inversion polymorphisms on all five chromosome arms of 157 haploid genomes from two African populations. Our observations show significantly increased numbers of elements within the regions associated with the least common, or minority arrangements, in natural inversion polymorphisms.

scholarly journals The P-element strikes again: the recent invasion of naturalDrosophila simulanspopulations

2015 ◽  
Author(s):  
Robert Kofler ◽  
Tom Hill ◽  
Viola Nolte ◽  
Andrea Betancourt ◽  
Christian Schlötterer
Keyword(s):  
Drosophila Melanogaster ◽  
Defense Mechanisms ◽  
Natural Populations ◽  
Genomic Data ◽  
P Element ◽  
Expression Data ◽  
Transfer Event ◽  
P Elements ◽  
Origins Of Replication ◽  
Close Relatives

The P-element is one of the best understood eukaryotic transposable elements. It invadedDrosophila melanogasterpopulations within a few decades, but was thought to be absent from close relatives, includingD. simulans. Five decades after the spread inD. melanogaster, we provide evidence that the P-element has also invadedD. simulans. P-elements inD. simulansappear to have been acquired recently fromD. melanogasterprobably via a single horizontal transfer event. Expression data indicate that the P-element is processed in the germline ofD. simulans, and genomic data show an enrichment of P-element insertions in putative origins of replication, similar to that seen inD. melanogaster. This ongoing spread of the P-element in natural populations provides an unique opportunity to understand the dynamics of transposable element spreads and the associated piRNA defense mechanisms.

scholarly journals Transposable element-induced fitness mutations in Drosophila melanogaster

1986 ◽  
Vol 48 (2) ◽  
pp. 77-87 ◽  
Author(s):  
Trudy F. C. Mackay
Keyword(s):  
Drosophila Melanogaster ◽  
Extraction Procedure ◽  
Natural Populations ◽  
P Element ◽  
Recessive Lethal ◽  
P Elements ◽  
Fitness Traits ◽  
Mutational Effect ◽  
Evolution Of Transposable Elements ◽  
Mutational Variance

SummaryP element mutagenesis was used to contaminate M strain second chromosomes with P elements. The contaminated lines were compared to uncontaminated control lines for homozygous and heterozygous fitness and its components. Mean homozygous fitness, viability and fertility of chromosome lines contaminated with P elements is decreased relative to the uncontaminated control lines by, respectively, 55, 28 and 40%. Variance among contaminated homozygous lines of total fitness increases by a factor of 1·5, variance of viability by a factor of 5·9, and variance of fertility by a factor of 1·9, compared to variance of these traits among the population of uncontaminated homozygous chromosomes. Estimates of P-element-induced mutational variance among second chromosome lines for homozygous fitness, viability and fertility are, respectively, 2 × 10−2, 5 × 10−2 and 2 × 10−2. This magnitude of mutational effect is equivalent, in terms of incidence of induced recessive lethal chromosomes and D:L ratio, to a dose of approximately 1·0–2·5 × 10−3 m EMS. The distributions of fitness traits among M-derived second chromosome homozygous lines contaminated with P elements are remarkably similar in many regards to distributions of fitness and viability of chromosomal homozygotes derived from natural Drosophila populations. It is possible that a proportion of the fitness variation previously observed (reviewed by Simmons & Crow, 1977) following homozygosis of wild chromosomes was not present in the natural populations, but was generated by P-element transposition during the chromosome extraction procedure. P-element-induced fitness mutations appear to be completely recessive. Implications for models of evolution of transposable elements are discussed.

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