scholarly journals Independent Tryptophan pathway in Trichoderma asperellum and T koningiopsis: New insights with bioinformatic and molecular analysis

2020 ◽  
Author(s):  
Uribe Bueno Mariana ◽  
JL Hernández-Mendoza ◽  
García Carlos Armando ◽  
Ancona Veronica ◽  
Violeta Larios-Serrato
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Bioinformatic Analysis ◽  
Trichoderma Asperellum ◽  
The Third ◽  
Nitrogen Fixers ◽  
Tryptophan Pathway ◽  
Three Stages ◽  
Exogenous Origin ◽  
Chorismic Acid

ABSTRACTThe synthesis of Indole Acetic Acid from tryptophan has been described in plants, fungi and bacteria; it is thus known as tryptophan-dependent indole acetic acid. Four possible pathways of IAA formation have been described, including the indole acetonitrile acid (IAN), indole acetamide (IAM), indole-pyruvic (IAP) and tryptamine (TRM) pathways. Of these, the indole acetonitrile pathway is particularly important because when this compound is transformed into IAA, a nitrogenated molecule is released. The microorganisms that have this pathway are thus called nitrogen fixers. There is another little-studied pathway called TRP-Independent, so-called because the IAA that is formed in it can have an exogenous origin, chorismic acid (CHA), and it enters the pathway through anthranilic acid (ANA). The TRP-Independent pathway is made up of three stages. The first from CHA to ANA, the second from AA to IAA and the third from TRP to ANA through Kynurenine (KYN). This work describes the different stages of the pathway, as well as the enzymes and the genes that control the production of IAA, using a bioinformatic analysis of the genes involved, which were identified by PCR. An expression analysis showed that only T asperellum has the necessary genes to incorporate ANA into the TRP-I pathway and synthesize IAA through it. The analysis also detected the gene that regulates anthranilate phosphoribosyl transferase (AFT), an enzyme necessary for the synthesis of AIA from ANA; the presence of this gene was confirmed in the two species analyzed.

scholarly journals The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea

2017 ◽  
Vol 1 (1) ◽  
pp. 31-35
Author(s):  
LINA HERLINA ◽  
KRISPINUS KEDATI PUKAN ◽  
DEWI MUSTIKANINGTYAS
Keyword(s):  
Acetic Acid ◽  
Arachis Hypogaea ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Lateral Roots ◽  
Morphological Characteristics ◽  
Bioactive Metabolites ◽  
Biological Compounds ◽  
Three Stages

Herlina L, Pukan KK, Mustikaningtyas D. 2017. The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea. Cell Bio Dev 1: 31-35. Endophytic bacteria are bacteria living in plant tissue and forming colony without harms the host. Every cormophyte, plants that have a stem and root, may contain some endophytic bacteria which can produce biological compounds or secondary metabolites. The objective of the study was to obtain endophytic bacteria isolates from peanut plants (Arachis hypogaea) at three locations, and to test in vitro the ability of endophytic bacteria isolates for producing IAA, and also to analyze IAA on the growth and development of mungbean plants. The study was carried out in three stages; the first was the isolation of endophytic bacteria from the leaves, stems, and roots; the second was the test of in vitro endophytic bacteria isolates to know the ability of IAA production. IAA assay was measured by using a spectrophotometer with a wavelength of 535 nm, and the third was the introduction of IAA-producing endophytic bacteria in mungbean. The parameters observed were the length of sprouts and the number of lateral roots. The results showed that 16 isolates were selected based on IAA-producing ability. The isolates could produce different IAA with different morphological characteristics. After the fourth day of incubation, the highest and the lowest of IAA amount were 69.68 (mg L-1) and 8.50 (mg L-1) respectively. Isolates that produce high IAA levels are applied to mungbeans, it affects the number of lateral roots but it does not have effect on the length of the sprouts. DM and K1K1 isolates have the effect of increasing lateral root formation and are expected to be potential sources of bioactive metabolites.

The Coherence of Mary Barton: Romance, Realism, and Utopia

2015 ◽  
Vol 5 (3) ◽  
pp. 185-200
Author(s):  
Robert Z. Birdwell
Keyword(s):  
Thomas Carlyle ◽  
The Novel ◽  
Second Stage ◽  
The Third ◽  
Third Stage ◽  
Three Stages ◽  
Mode Of Production

Critics have argued that Elizabeth Gaskell's first novel, Mary Barton (1848), is split by a conflict between the modes of realism and romance. But the conflict does not render the novel incoherent, because Gaskell surpasses both modes through a utopian narrative that breaks with the conflict of form and gives coherence to the whole novel. Gaskell not only depicts what Thomas Carlyle called the ‘Condition of England’ in her work but also develops, through three stages, the utopia that will redeem this condition. The first stage is romantic nostalgia, a backward glance at Eden from the countryside surrounding Manchester. The second stage occurs in Manchester, as Gaskell mixes romance with a realistic mode, tracing a utopian drive toward death. The third stage is the utopian break with romantic and realistic accounts of the Condition of England and with the inadequate preceding conceptions of utopia. This third stage transforms narrative modes and figures a new mode of production.

STRATEGI MAKSIMALISASI ANGGARAN BELANJA PADA BALAI PENELITIAN DAN PENGEMBANGAN LINGKUNGAN HIDUP DAN KEHUTANAN MANOKWARI

2016 ◽  
Vol 8 (2) ◽  
Author(s):  
Arif Hasan ◽  
Dedi Budiman Hakim ◽  
Irdika Mansur
Keyword(s):  
Decision Making ◽  
Descriptive Analysis ◽  
Secondary Data ◽  
Primary Data ◽  
Fiscal Year ◽  
The Third ◽  
Swot Matrix ◽  
Three Stages ◽  
Using Data

This study aims to analyze causes of the low uptake of the budget and formulate a strategy of maximizing the absorption of expenditure on Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Manokwari. Respondents involved are 20 people that consist of: treasury officials and holder output of activity. The data used were secondary data in the form of reports on budget realization (LRA) quarter I, II, III and IV of the fiscal year 2011 to 2015, and the primary data were in the form of interviews with the help of a questionnaire. While the analysis of the data used was descriptive analysis using data tabulation, and the analysis of the three stages strategy of the decision making used IFE and EFE matrix, SWOT matrix and QSPM matrix.The results showed that there are 19 factors causing low of budget absorption until the end of the third quarter, and there were 10 drafts of policy as a strategy for maximizing the absorption of the budget on Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Manokwari.ABSTRAKPenelitian ini bertujuan untuk menganalisis penyebab rendahnya penyerapan anggaran belanja dan merumuskan strategi maksimalisasi penyerapan anggaran belanja pada Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Manokwari. Responden yang terlibat adalah 20 orang yaitu pejabat perbendaharaan dan pemegang output kegiatan. Data yang digunakan adalah data sekunder berupa laporan realisasi anggaran (LRA) triwulan I, II, III dan IV tahun anggaran 2011 sampai 2015, dan data primer berupa wawancara dengan bantuan kuesioner. Sedangkan analisis data yang digunakan adalah analisis deskriptif menggunakan analisis tabulasi, dan analisis analisis strategi tiga tahap pengambilan keputusan menggunakan matriks IFE dan EFE, matriks SWOT dan matriks QSPM. Hasil penelitian menunjukkan bahwa terdapat 19 faktor penyebab rendahnya penyerapan anggaran belanja sampai akhir triwulan III, dan terdapat 10 rancangan kebijakan sebagai strategi maksimalisasi penyerapan anggaran belanja di Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Manokwari.

Isolation and identification of IAA producing endosymbiotic bacteria from Gracillaria corticata (J. Agardh)

2016 ◽  
Vol 5 (12) ◽  
pp. 5179
Author(s):  
Ilahi Shaik* ◽  
P. Janakiram ◽  
Sujatha L. ◽  
Sushma Chandra
Keyword(s):  
Acetic Acid ◽  
Culture Filtrate ◽  
Indole Acetic Acid ◽  
Shoot Growth ◽  
High Salinity ◽  
Saline Soils ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Endosymbiotic Bacteria ◽  
Root And Shoot Growth

Indole acetic acid is a natural phytohormone which influence the root and shoot growth of the plants. Six (GM1-GM6) endosymbiotic bacteria are isolated from Gracilaria corticata and screened for the production of IAA out of six, three bacterial strains GM3, GM5 and GM6 produced significant amount of IAA 102.4 µg/ml 89.40 µg/ml 109.43 µg/ml respectively. Presence of IAA in culture filtrate of the above strains is further analyzed and confirmed by TLC. As these bacterial strains, able to tolerate the high salinity these can be effectively used as PGR to increase the crop yield in saline soils.

scholarly journals Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Durum Wheat ◽  
Growth And Development ◽  
Indole Acetic Acid ◽  
Greenhouse Experiment ◽  
Hydrocyanic Acid ◽  
In Vitro Assays

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

Specificity of Phospholipid Binding to Indole Acetic Acid and Other Auxins

1974 ◽  
Vol 29 (1-2) ◽  
pp. 39-41 ◽  
Author(s):  
H. Veen
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phospholipid Binding ◽  
Primary Action

Abstract Studies in vitro on the binding of phospholipids with IAA, α-NAA and some of their biologically inactive analogues demonstrate that the observed interaction between IA A and lecithin is not related to the primary action of the hormone in plant growth.

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