scholarly journals UBP12 and UBP13 deubiquitinases destabilize the CRY2 blue-light receptor to regulate growth

2021 ◽  
Author(s):  
Louise Norén Lindbäck ◽  
Oliver Artz ◽  
Amanda Ackermann ◽  
Ullas V. Pedmale
Keyword(s):  
Blue Light ◽  
General View ◽  
Developmental Defects ◽  
Protein Protein Interaction ◽  
Protein Levels ◽  
Blue Light Receptor ◽  
Control Growth ◽  
Light Quantity ◽  
Fine Tune ◽  
Insight Into

ABSTRACTAll organisms undergo growth, which is precisely controlled by exogenous and endogenous signals. Unchecked growth often leads to neoplasia and other developmental defects, severely affecting an organism’s fitness. Light is a vital exogenous signal sensed by cryptochrome (CRY) blue light receptors to modulate growth and the circadian clock in plants and animals. Yet, how CRYs interpret light quantity to regulate growth in plants remains poorly understood. We show that UBP12 and UBP13 deubiquitinases physically interact with CRY2 in light. UBP12/13 negatively regulated CRY2 protein levels by promoting its ubiquitination and turnover to fine-tune growth. Unexpectedly, the destabilization of CRY2 by UBP12/13 is contrary to the general view that deubiquitinases stabilize proteins by preventing their degradation. Growth and development were explicitly affected in blue light when UBP12/13 was disrupted or overexpressed, indicating their role alongside CRY2. UBP12/13 also interacted and stabilized COP1, which is partially required for the turnover of CRY2. Despite decades of studies on deubiquitinases, the knowledge on how they are regulated is limited. Our study offers an insight into how exogenous signals and their receptors regulate deubiquitinase activity by protein-protein interaction. Altogether, our results provide a new module of cryptochromes and deubiquitinases in sensing and interpreting light cues to control growth at the most appropriate time.

scholarly journals Gamblers’ Perception of the Playscan Risk Assessment: A Mixed-Methods Study

2021 ◽  
Author(s):  
David Forsström ◽  
Alexander Rozental ◽  
Emma Wiklund ◽  
Per Carlbring ◽  
Philip Lindner
Keyword(s):  
Risk Assessment ◽  
Mixed Methods ◽  
Structural Equation ◽  
Responsible Gambling ◽  
Gambling Behavior ◽  
Mixed Methods Study ◽  
Equation Modeling ◽  
General View ◽  
Insight Into ◽  
Qualitative Analyses

AbstractResponsible gambling (RG) tools are globally widespread; they aim to prevent or decrease the harm caused by gambling. However, existing research suggests that several included features do not decrease gambling or significantly reduce the subsequent harm. Most of the previous studies have used gambling data to understand the changes in gambling behavior. However, the literature lacks research regarding gamblers’ experience and perception of RG tools, which may provide insight into increasing the usage and effectiveness of RG tools. This mixed-methods study aimed to explore gamblers’ perception of their risk assessment in the RG tool Playscan regarding developing harmful gambling problems. Overall, 757 participants rated the perceived accuracy of their risk assessment and their perception of the overall RG tool that conducted the assessment. Participants were also allowed to leave a comment providing feedback, which was analyzed using thematic analysis. Quantitative data was analyzed using logistic regression and structural equation modeling. Qualitative analyses revealed that most of the participants were pleased with the risk assessment and found it helpful. Moderated mediation analysis showed that participants’ assessment agreement partially mediated the association between expressing a negative view and their general view of Playscan. These results highlight the need to decrease the level of disagreement for promoting a better general view of RG tools to potentially increase their usage and effectiveness.

scholarly journals Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker

2017 ◽  
Vol 217 (2) ◽  
pp. 779-793 ◽  
Author(s):  
Rebecca C. Adikes ◽  
Ryan A. Hallett ◽  
Brian F. Saway ◽  
Brian Kuhlman ◽  
Kevin C. Slep
Keyword(s):  
Blue Light ◽  
Actin Binding ◽  
Cross Linking ◽  
Dependent Manner ◽  
Exclusion Zone ◽  
Actin Networks ◽  
Drosophila Melanogaster S2 Cells ◽  
Actin Binding Domain ◽  
Specific Factors ◽  
Insight Into

We developed a novel optogenetic tool, SxIP–improved light-inducible dimer (iLID), to facilitate the reversible recruitment of factors to microtubule (MT) plus ends in an end-binding protein–dependent manner using blue light. We show that SxIP-iLID can track MT plus ends and recruit tgRFP-SspB upon blue light activation. We used this system to investigate the effects of cross-linking MT plus ends and F-actin in Drosophila melanogaster S2 cells to gain insight into spectraplakin function and mechanism. We show that SxIP-iLID can be used to temporally recruit an F-actin binding domain to MT plus ends and cross-link the MT and F-actin networks. Cross-linking decreases MT growth velocities and generates a peripheral MT exclusion zone. SxIP-iLID facilitates the general recruitment of specific factors to MT plus ends with temporal control enabling researchers to systematically regulate MT plus end dynamics and probe MT plus end function in many biological processes.

Light-dependent suppression of COP1 multimeric complex formation is determined by the blue-light receptor FKF1 in Arabidopsis

2019 ◽  
Vol 508 (1) ◽  
pp. 191-197 ◽  
Author(s):  
Byoung-Doo Lee ◽  
Joon-Yung Cha ◽  
Mi Ri Kim ◽  
Gyeong-Im Shin ◽  
Nam-Chon Paek ◽  
...  
Keyword(s):  
Complex Formation ◽  
Blue Light ◽  
Blue Light Receptor ◽  
Multimeric Complex ◽  
Light Receptor

scholarly journals A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

2022 ◽  
Vol 8 (1) ◽  
pp. 50
Author(s):  
Yifan Li ◽  
Xiya Meng ◽  
Degang Guo ◽  
Jia Gao ◽  
Qiwei Huang ◽  
...  
Keyword(s):  
Blue Light ◽  
Stress Responses ◽  
Stress Resistance ◽  
Low Cost ◽  
Hog Pathway ◽  
Precise Control ◽  
Blue Light Receptor ◽  
Expression Of Genes ◽  
Low Cost Strategy ◽  
Stress Related Genes

Light is perceived by photoreceptors in fungi and further integrated into the stress-activated MAPK HOG pathway, and thereby potentially activates the expression of genes for stress responses. This indicates that the precise control of light conditions can likely improve the conidial yield and stress resistance to guarantee the low cost and long shelf life of Trichoderma-based biocontrol agents and biofertilizers. In this study, effects of wavelengths and intensities of light on conidial yield and stress tolerance to osmotic, oxidative and pH stresses in Trichoderma guizhouense were investigated. We found that 2 μmol photons/(m2 × s) of blue light increased the conidial yield more than 1000 folds as compared to dark condition and simultaneously enhanced conidial stress resistance. The enhanced conidial stress resistance is probably due to the upregulated stress-related genes in blue light, which is under the control of the blue light receptor BLR1 and the MAP kinase HOG1.

scholarly journals The entero-insular axis: a journey in the physiopathology of diabetes

2020 ◽  
Vol 1 (6) ◽  
Author(s):  
Roberta Malaguarnera ◽  
Alessandra Scamporrino ◽  
Agnese Filippello ◽  
Stefania Di Mauro ◽  
Alessandro Minardo ◽  
...  
Keyword(s):  
Scientific Progress ◽  
Blood Lipid ◽  
Modern Medicine ◽  
General View ◽  
Protein Levels ◽  
Therapeutic Algorithms ◽  
Other Substances ◽  
Islet Hormone ◽  
New Perspective ◽  
Complex Scenario

Glycemic homeostasis is an essential mechanism for the proper working of an organism. However, balance in blood lipid and protein levels also plays an important role. The discovery of the hormone insulin and the description of its function for glycemic control made fundamental scientific progress in this field. However, since then our view of the problem has been deeply influenced only in terms of glucose and insulin (in an insulin-centric and glucose-centric way). Based on recent scientific discoveries, a fine and sophisticated network of hormonal and metabolic interactions, involving almost every apparatus and tissue of the human body, has been theorized. Efficient metabolic homeostasis is founded on these intricate interactions. Although it is still not fully defined, this complex network can undergo alterations that lead to metabolic disorders such as diabetes mellitus (DM). The endocrine pancreas plays a crucial role in the metabolic balance of an organism, but insulin is just one of the elements involved and each single pancreatic islet hormone is worthy of our concern. Moreover, pancreatic hormones need to be considered in a general view, concerning both their systemic function as direct mediators and as hormones, which, in turn, are regulated by other hormones or other substances. This more complex scenario should be taken into account for a better understanding of the pathophysiology and the therapeutic algorithms of DM. As a consequence, improvements in modern medicine could help to contemplate this new perspective. This review is focused on some aspects of gut-pancreas interaction, aiming to integrate this synergy into a wider context involving other organs and tissues.

scholarly journals Role of Short-wavelength Blue Light in the Formation of Cataract and Expression of Caspase-1, -11 and Gasdermin D in rat Lens Epithelium Cells: Insight into the Novel Pathogenesis of Cataract.

2019 ◽  
Author(s):  
Yamin Wang ◽  
Min Zhang ◽  
Ying Sun ◽  
Xiaohui Wang ◽  
Zhaowei Song ◽  
...  
Keyword(s):  
Blue Light ◽  
Short Wavelength ◽  
Light Exposure ◽  
The Novel ◽  
Blue Led ◽  
Caspase 1 ◽  
Epithelium Cells ◽  
Experimental Group ◽  
Insight Into

Abstract Background Cataracts have been verified to be associated with a number of risk factors. The sun and artificial light sources, including light-emitting diode (LED) and fluorescent light tubes, are the primary sources of short-wavelength blue light. With the increasing popularity of blue-rich LED-backlit display devices, our eyes are now exposed to more short-wavelength blue light than they were in the past. The goal of this study was to evaluate the role of short-wavelength blue light in the formation of cataract. Additionally, the pathogenesis of cataracts after short-wavelength light exposure was investigated.Methods SD rats were randomly divided into 2 main groups: a control group (10 rats each for the 4-, 8-, and 12-week groups) and an experimental group (10 rats each for the 4-, 8-, and 12-week groups). The rats in the experimental group were exposed to a short-wavelength blue LED lamp for 12 hours per day. After exposure to the blue LED lamp, the rats were maintained in total darkness for 12 hours, after which a 12-hour light/dark cycle was resumed. The intensity of the lamp was 3000 lux. At the end of the short-wavelength blue LED lamp exposure (for 4, 8, and 12 weeks), the expression levels of caspase-1, caspase-11 and gasdermin D (GSDMD) in rat epithelium cells (LECs) were examined in rat epithelial cells (LECs) using qRT-PCR and Western blotting analyses. Results After 6 weeks, cataracts had developed in the experimental rats (4/20 eyes). The clarity of the lens then gradually worsened with the duration of exposure. Twelve weeks later, all of the rat eyes had developed cataracts. Then the expression levels of caspase-1, caspase-11 and GSDMD at 4, 8, and 12 weeks were significantly higher in samples from rats exposed to a short-wavelength blue LED lamp than samples from control rat (p˂0.05). Conclusion The data indicate that pyroptosis play a key role of in cataracts induced by short-wavelength blue light exposure, highlighting caspase-1, caspase-11 and GSDMD as possible therapeutic targets for cataract treatment. This study might provide new insight into the novel pathogenesis of cataracts.

Sign in / Sign up

Export Citation Format

Share Document