scholarly journals Enviromic assembly increases accuracy and reduces costs of the genomic prediction for yield plasticity

2021 ◽  
Author(s):  
Germano MF Costa-Neto ◽  
Jose M F Crossa ◽  
Roberto F Fritsche-Neto
Keyword(s):  
Phenotypic Variation ◽  
Field Trials ◽  
Proof Of Concept ◽  
Tropical Maize ◽  
Early Screening ◽  
Environmental Similarity ◽  
Genetic And Environmental Factors ◽  
Growing Conditions ◽  
Algorithm Scheme

Quantitative genetics states that phenotypic variation is a consequence of genetic and environmental factors and their subsequent interaction. Here, we present an enviromic assembly approach, which includes the use of ecophysiology knowledge in shaping environmental relatedness into whole-genome predictions (GP) for plant breeding (referred to as E-GP). We propose that the quality of an environment is defined by the core of environmental typologies (envirotype) and their frequencies, which describe different zones of plant adaptation. From that, we derive markers of environmental similarity cost-effectively. Combined with the traditional genomic sources (e.g., additive and dominance effects), this approach may better represent the putative phenotypic variation across diverse growing conditions (i.e., phenotypic plasticity). Additionally, we couple a genetic algorithm scheme to design optimized multi-environment field trials (MET), combining enviromic assembly and genomic kinships to provide in-silico realizations of the future genotype-environment combinations that must be phenotyped in the field. As a proof-of-concept, we highlight E-GP applications: (1) managing the lack of phenotypic information in training accurate GP models across diverse environments and (2) guiding an early screening for yield plasticity using optimized phenotyping efforts. Our approach was tested using two non-conventional cross-validation schemes to better visualize the benefits of enviromic assembly in sparse experimental networks. Results on tropical maize show that E-GP outperforms benchmark GP in all scenarios and cases tested. We show that for training accurate GP models, the genotype-environment combinations' representativeness is more critical than the MET size. Furthermore, we discuss theoretical backgrounds underlying how the intrinsic envirotype-phenotype covariances within the phenotypic records of (MET) can impact the accuracy of GP and limits the potentialities of predictive breeding approaches. The E-GP is an efficient approach to better use environmental databases to deliver climate-smart solutions, reduce field costs, and anticipate future scenarios.

scholarly journals Enviromic Assembly Increases Accuracy and Reduces Costs of the Genomic Prediction for Yield Plasticity in Maize

2021 ◽  
Vol 12 ◽  
Author(s):  
Germano Costa-Neto ◽  
Jose Crossa ◽  
Roberto Fritsche-Neto
Keyword(s):  
Phenotypic Variation ◽  
Genomic Prediction ◽  
Proof Of Concept ◽  
Tropical Maize ◽  
Training Set ◽  
Early Screening ◽  
Environmental Similarity ◽  
Genetic And Environmental Factors ◽  
Growing Conditions

Quantitative genetics states that phenotypic variation is a consequence of the interaction between genetic and environmental factors. Predictive breeding is based on this statement, and because of this, ways of modeling genetic effects are still evolving. At the same time, the same refinement must be used for processing environmental information. Here, we present an “enviromic assembly approach,” which includes using ecophysiology knowledge in shaping environmental relatedness into whole-genome predictions (GP) for plant breeding (referred to as enviromic-aided genomic prediction, E-GP). We propose that the quality of an environment is defined by the core of environmental typologies and their frequencies, which describe different zones of plant adaptation. From this, we derived markers of environmental similarity cost-effectively. Combined with the traditional additive and non-additive effects, this approach may better represent the putative phenotypic variation observed across diverse growing conditions (i.e., phenotypic plasticity). Then, we designed optimized multi-environment trials coupling genetic algorithms, enviromic assembly, and genomic kinships capable of providing in-silico realization of the genotype-environment combinations that must be phenotyped in the field. As proof of concept, we highlighted two E-GP applications: (1) managing the lack of phenotypic information in training accurate GP models across diverse environments and (2) guiding an early screening for yield plasticity exerting optimized phenotyping efforts. Our approach was tested using two tropical maize sets, two types of enviromics assembly, six experimental network sizes, and two types of optimized training set across environments. We observed that E-GP outperforms benchmark GP in all scenarios, especially when considering smaller training sets. The representativeness of genotype-environment combinations is more critical than the size of multi-environment trials (METs). The conventional genomic best-unbiased prediction (GBLUP) is inefficient in predicting the quality of a yet-to-be-seen environment, while enviromic assembly enabled it by increasing the accuracy of yield plasticity predictions. Furthermore, we discussed theoretical backgrounds underlying how intrinsic envirotype-phenotype covariances within the phenotypic records can impact the accuracy of GP. The E-GP is an efficient approach to better use environmental databases to deliver climate-smart solutions, reduce field costs, and anticipate future scenarios.

scholarly journals Technological quality of common buckwheat (Fagopyrum esculentum Moench.)

2011 ◽  
Vol 48 (No. 6) ◽  
pp. 279-284 ◽  
Author(s):  
J. Kalinová ◽  
J. Moudrý ◽  
V. Čurn
Keyword(s):  
Field Trials ◽  
Quality Parameters ◽  
Common Buckwheat ◽  
Nitrogen Fertilisation ◽  
Volume Weight ◽  
Technological Value ◽  
Technological Quality ◽  
Growing Conditions ◽  
Fagopyrum Esculentum Moench

Seven cultivars of common buckwheat were tested in field trials under two levels of nitrogen fertilisation on two experimental sites during 1998&ndash;2000. The aim of the experiments was to evaluate the influence of cultivar, nutrition and year on main technological quality parameters (thousand achenes weight, volume weight, proportion of fractions on sieves 4.5 and 4 mm, proportion of husks and yield of groats). The differences were observed between buckwheat cultivars in all observed parameters of technological value. Nitrogen fertilisation before sowing (50 kg.ha<sup>&ndash;1</sup>) did not influence any parameter. On the contrary, buckwheat technological value was influenced by sequence weather (particularly rainfalls) during flowering and achenes formation periods (July). The influence of year was manifested especially on development of endosperm and husks of achenes. Better growing conditions on experimental site Uhř&iacute;něves caused lower values of volume weight, lower proportion of pericarp (husks) and considerably higher proportion of fraction over 4.5 mm.

scholarly journals Chemical composition and biological properties in Mentha spicata under conventional and organic fertilization

2021 ◽  
Vol 13 (2) ◽  
Author(s):  
Diego Ariel Meloni ◽  
José Aliçandro Bezerra da Silva ◽  
Anahí Bordón ◽  
Julia Andrea Lescano ◽  
Rosa Elizabeth Beltrán
Keyword(s):  
Chemical Properties ◽  
Field Trials ◽  
Biological Properties ◽  
Soil Conditions ◽  
Organic Fertilization ◽  
Mentha Spicata ◽  
Food Industries ◽  
Yield And Quality ◽  
Genetic And Environmental Factors

Introduction: Spearmint (Mentha spicata) is widely used in the pharmaceutical and food industries, thanks to chemical properties largely influenced by genetic and environmental factors, especially soil conditions. Objective: To determine the effect of conventional and organic fertilization on the chemical and biological properties of M. spicata. Methods: We conducted field trials in a randomized block experimental design, with four replications, using unfertilized crops and crops fertilized with urea (0,15 t ha-1) or vermicompost (5 and 10 t ha-1). Results: All fertilization treatments increased essential oil yield, carvone content, and total polyphenol concentration with respect to the control. They also increased the antioxidant capacity and the inhibitory activity of the acetylcholinesterase, butyrylcholinesterase, α-amylase, and α-glycosidase enzymes. The effect was more marked in the crop fertilized with 5 t ha-1 vermicompost. Conclusions: Both conventional and organic fertilization increase the yield and quality of M. spicata essential oils. However, organic fertilization with 5 t ha-1 vermicompost yields rich total polyphenols and carvone. This improves antioxidant and medicinal properties, acting on enzymes related to Alzheimer's disease and diabetes.

PENGEMBANGAN MEDIA PEMBELAJARAN INTERAKTIF TEKNIK PEMESINAN BUBUT

2020 ◽  
Vol 6 (2) ◽  
pp. 169
Author(s):  
Philip Nababan ◽  
Efendi Napitupulu ◽  
R Mursid
Keyword(s):  
Student Learning ◽  
Learning Outcomes ◽  
Interactive Learning ◽  
Descriptive Analysis ◽  
Student Learning Outcomes ◽  
Field Trials ◽  
Student Responses ◽  
Analysis Techniques ◽  
Qualitative Descriptive

Abstrak: Penelitian ini bertujuan untuk: (1) Mengetahui tanggapan siswa terhadap kualitas media pembelajaran interaktif pada pembelajaran Teknik Pemesinan Bubut. (2) Mengetahui keefektifan media pembelajaran interaktif pada pembelajaran Teknik Pemesinan Bubut pada siswa program keahlian Teknik Pemesinan. Jenis penelitian ini adalah penelitian pengembangan. Data tentang kualitas produk pengembangan ini dikumpulkan dengan angket dan dianalisis dengan teknik analisis deskriptif kualiatatif. Hasil penelitian menunjukkan bahwa; (1) uji ahli materi pelajaran Teknik Pemesinan Bubut berada pada kualifikasi sangat baik (88,92%), (2) uji ahli desain pembelajaran berada pada kualifikasi sangat baik (85,21%), (3) uji ahli rekayasa perangkat lunak berada pada kualifikasi sangat baik (84,03%), (4) uji coba perorangan berada pada kualifikasi sangat baik (88,75%), (5) uji coba kelompok kecil berada pada kualifikasi sangat baik (91,35%) dan (5) uji coba lapangan berada pada kualifikasi sangat baik (88,31%). Hasil pengujian hipotesis membuktikan bahwa terdapat perbedaan antara hasil belajar siswa yang menggunakan media pembelajaran interaktif  dengan hasil belajar siswa yang menggunakan buku teks. Hal ini ditunjukkan dengan hasil pengolahan data diperoleh  thitung sebesar 4,68 dan ttabel sebesar 1,67 pada taraf kepercayaan 95 persen. Maka diperoleh bahwa thitung> ttabel. Disimpulkan bahwa  hasil belajar siswa yang menggunakan media pembelajaran interaktif dengan efektifitas sebesar 72,77 %. lebih tinggi dari hasil belajar siswa yang diajar dengan pembelajaran menggunakan buku teks dengan efektifitas sebesar 62,13%. Kata Kunci: media pembelajaran interaktif, teknik pemesinan bubut Abstract: This study aims to: (1) Determine student responses to the quality of interactive learning media on learning Lathe Machining Techniques. (2) Knowing the effectiveness of interactive learning media on learning of Machining Lathe in students of Machining Engineering expertise program. This type of research is development research. Data about the quality of this development product was collected by a questionnaire and analyzed by qualitative descriptive analysis techniques. The results showed that; (1) Lathe machining engineering subject matter expert test is in very good qualification (88.92%), (2) learning design expert test is in very good qualification (85.21%), (3) software engineering expert test is in in very good qualifications (84.03%), (4) individual trials were in very good qualifications (88.75%), (5) small group trials were in very good qualifications (91.35%) and (5 ) field trials are in very good qualifications (88.31%). Hypothesis testing results prove that there are differences between student learning outcomes using interactive learning media with student learning outcomes using textbooks. This is indicated by the results of data processing obtained by tcount of 4.68 and ttable of 1.67 at a confidence level of 95 percent. Then it is obtained that tcount> ttable. It was concluded that student learning outcomes using interactive learning media with an effectiveness of 72.77%. higher than student learning outcomes taught by learning to use textbooks with an effectiveness of 62.13%. Keywords: interactive learning media, lathe machining techniques

Assessment of the quality of tubers of potatoes of varieties of different ripeness group depending on growing conditions

2019 ◽  
Vol 94 (1) ◽  
pp. 274-284
Author(s):  
R. O. Mialkovskyi ◽  
◽  
V.I. Ovcharuk ◽  
P. V. Bezvikonnyy ◽  
V. S. Kravchenko V. S. ◽  
...  
Keyword(s):  
Growing Conditions

scholarly journals The Impact of Different Environmental Conditions during Vegetative Propagation on Growth, Survival, and Biochemical Characteristics in Populus Hybrids in Clonal Field Trial

Forests ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 892
Author(s):  
Valda Gudynaitė-Franckevičienė ◽  
Alfas Pliūra
Keyword(s):  
Climate Change ◽  
Phenotypic Plasticity ◽  
Environmental Conditions ◽  
Vegetative Propagation ◽  
Genotypic Variation ◽  
Hybrid Poplar ◽  
Field Trials ◽  
Biochemical Traits ◽  
Suburban Areas ◽  
Growing Conditions

To have a cleaner environment, good well-being, and improve the health of citizens it is necessary to expand green urban and suburban areas using productive and adapted material of tree species. The quality of urban greenery, resistance to negative climate change factors and pollution, as well as efficiency of short-rotation forestry in suburban areas, depends primarily on the selection of hybrids and clones, suitable for the local environmental conditions. We postulate that ecogenetic response, phenotypic plasticity, and genotypic variation of hybrid poplars (Populus L.) grown in plantations are affected not only by the peculiarities of hybrids and clones, but also by environmental conditions of their vegetative propagation. The aim of the present study was to estimate growth and biochemical responses, the phenotypic plasticity, genotypic variation of adaptive traits, and genetically regulated adaptability of Populus hybrids in field trials which may be predisposed by the simulated contrasting temperature conditions at their vegetative propagation phase. The research was performed with the 20 cultivars and experimental clones of one intraspecific cross and four different interspecific hybrids of poplars propagated under six contrasting temperature regimes in phytotron. The results suggest that certain environmental conditions during vegetative propagation not only have a short-term effect on tree viability and growth, but also can help to adapt to climate change conditions and grow successfully in the long-term. It was found that tree growth and biochemical traits (the chlorophyll A and B, pigments content and the chlorophyll A/B ratio) of hybrid poplar clones grown in field trials, as well as their traits’ genetic parameters, were affected by the rooting-growing conditions during vegetative propagation phase. Hybrids P. balsamifera × P. trichocarpa, and P. trichocarpa × P. trichocarpa have shown the most substantial changes of biochemical traits across vegetative propagation treatments in field trial. Rooting-growing conditions during vegetative propagation had also an impact on coefficients of genotypic variation and heritability in hybrid poplar clones when grown in field trials.

scholarly journals A prescribed walking regimen plus arginine supplementation improves function and quality of life for patients with pulmonary arterial hypertension: a pilot study

2017 ◽  
Vol 8 (1) ◽  
pp. 204589321774396 ◽  
Author(s):  
Mary Beth Brown ◽  
Attie Kempf ◽  
Catherine M. Collins ◽  
Gary M. Long ◽  
Matthew Owens ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Pulmonary Arterial Hypertension ◽  
Arterial Hypertension ◽  
Current Evidence ◽  
Proof Of Concept ◽  
Concept Study ◽  
Sf 36 ◽  
Pulmonary Arterial ◽  
Arginine Supplementation

Current evidence suggests that exercise training is beneficial in pulmonary arterial hypertension (PAH). Unfortunately, the standard supervised, hospital-based programs limit patient accessibility to this important intervention. Our proof-of-concept study aimed to provide insight into the usefulness of a prescribed walking regimen along with arginine supplementation to improve outcomes for patients with PAH. Twelve PAH patients (all women) in New York Heart Association (NYHA) functional class (FC) II (n = 7) or III (n = 5) and in stable condition for ≥ 3 months were enrolled. Patients performed home- and fitness-center- based walking at 65–75% heart rate (HR) reserve for 45 min, six sessions/week for 12 weeks. Concomitant L-arginine supplementation (6000 mg/day) was provided to maximize beneficial endothelial training adaptations. Cardiopulmonary exercise testing, 6-min walk testing (6MWT), echocardiography, laboratory studies, and quality of life (QoL) survey (SF-36) were performed at baseline and 12 weeks. Eleven patients completed the study (72 session adherence rate = 96 ± 3%). Objective improvement was demonstrated by the 6MWT distance (increased by 40 ± 13 m, P = 0.01), VO2max (increased by 2 ± 0.7 mL/kg/min, P = 0.02), time-to-VO2max (increased by 2.5 ± 0.6 min, P = 0.001), VO2 at anaerobic threshold (increased by 1.3 ± 0.5 mL/kg/min, P = 0.04), HR recovery (reduced by 68 ± 23% in slope, P = 0.01), and SF-36 subscales of Physical Functioning and Energy/Fatigue (increased by 70 ± 34% and 74 ± 34%, respectively, P < 0.05). No adverse events occurred, and right ventricular function and brain natriuretic peptide levels remained stable, suggesting safety of the intervention. This proof-of-concept study indicates that a simple walking regimen with arginine supplementation is a safe and efficacious intervention for clinically stable PAH patients, with gains in objective function and QoL measures. Further investigation in a randomized controlled trial is warranted.

The effect of motor imagery on quality of movement when performing reaching tasks in healthy subjects: A proof of concept

2021 ◽  
Author(s):  
Barbora Kolářová ◽  
Jim Richards ◽  
Hana Ondráčková ◽  
Klára Lippertová ◽  
Louise Connell ◽  
...  
Keyword(s):  
Motor Imagery ◽  
Healthy Subjects ◽  
Proof Of Concept ◽  
Reaching Tasks ◽  
Quality Of Movement

scholarly journals Enhancement of Region of Interest CT Reconstructions through Multimodal Data

2018 ◽  
Vol 4 (1) ◽  
pp. 331-335
Author(s):  
David Schote ◽  
Tim Pfeiffer ◽  
Georg Rose
Keyword(s):  
Substantial Reduction ◽  
Region Of Interest ◽  
Proof Of Concept ◽  
Multimodal Data ◽  
New Approach ◽  
Full Field ◽  
Lack Of Information ◽  
Ct Data ◽  
Flight Measurements

AbstractComputed tomography (CT) scans are frequently used intraoperatively, for example to control the positioning of implants during intervention. Often, to provide the required information, a full field of view is unnecessary. I nstead, the region-of-interest (ROI) imaging can be performed, allowing for substantial reduction in the applied X-ray dose. However, ROI imaging leads to data inconsistencies, caused by the truncation of the projections. This lack of information severely impairs the quality of the reconstructed images. This study presents a proof-of-concept for a new approach that combines the incomplete CT data with ultrasound data and time of flight measurements in order to restore some of the lacking information. The routine is evaluated in a simulation study using the original Shepp-Logan phantom in ROI cases with different degrees of truncation. Image quality is assessed by means of normalized root mean square error. The proposed method significantly reduces truncation artifacts in the reconstructions and achieves considerable radiation exposure reductions.

scholarly journals Thoracoscopic Ablation with Appendage Ligation versus Medical Therapy for Stroke Prevention a Proof-of-Concept Randomized Trial

2016 ◽  
Vol 11 (2) ◽  
pp. 99-105 ◽  
Author(s):  
Thomas M. Beaver ◽  
Vishnumurthy Shushrutha Hedna ◽  
Anna Y. Khanna ◽  
William M. Miles ◽  
Catherine C. Price ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Medical Management ◽  
Stroke Prevention ◽  
Normal Sinus Rhythm ◽  
Recurrent Stroke ◽  
Outcome Variable ◽  
Concept Design ◽  
Proof Of Concept ◽  
Surgical Morbidity

Objective Atrial fibrillation (AF) has a demonstrable effect on quality of life (QOL). Recurrent stroke occurs in 10% of patients with AF. The objective of this study was to demonstrate proof of concept that thoracoscopic pulmonary vein isolation and atrial appendage ligation (TPVIAL) could prevent recurrent stroke and could potentially improve QOL in patients with AF with a previous stroke. Methods The study was a National Institutes of Health-funded single-center proof-of-concept design that randomized 23 patients with AF-related stroke to TPVIAL (n = 12) or to medical management (n = 11). Quality of life was the primary outcome variable; secondary end points included restoration of rhythm, recurrent stroke, and surgical morbidity. Results Quality-of-life subscores at 3 and 6 months revealed improvements in energy and decreases in fatigue in the TPVIAL arm [baseline, 33 (19.8); 3 months, 49.5 (20.6), P = 0.01; 6 months, 55.5 (14.4), P = 0.03]. At 12-month follow-up, there were no recurrent strokes in the TPVIAL group. In the medically treated arm, two patients at 6 months (P = 0.22) and three total patients at 12 months (P = 0.09) had recurrent ischemic stroke. There was one death in the medical management arm. In the TPVIAL arm, no AF recurrence occurred in patients with paroxysmal AF, and one patient had recurrence of persistent and long-standing AF. Seven patients in the TPVIAL arm discontinued warfarin therapy for secondary stroke prevention. Conclusions This small proof-of-concept study showed that TPVIAL improved QOL on two subscores and restored normal sinus rhythm in all but one patient, and it showed the potential to prevent secondary stroke. A larger study will be needed.

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