scholarly journals Blood Unit Segments Accurately Represent the Biophysical Properties of Red Blood Cells in Blood Bags but Not Hemolysis

2021 ◽  
Author(s):  
Emel Islamzada ◽  
Kerryn Matthews ◽  
Erik Lamoureux ◽  
Simon P. Duffy ◽  
Mark D. Scott ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Clinical Studies ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Biophysical Properties ◽  
Distribution Width ◽  
Blood Unit ◽  
Blood Bag ◽  
Non Destructive

BACKGROUND: The biophysical properties of red blood cells (RBCs) provide potential biomarkers for the quality of donated blood. Blood unit segments provide a simple and non-destructive way to sample RBCs in clinical studies of transfusion efficacy, but it is not known whether RBCs sampled from segments accurately represent the biophysical properties of RBCs in blood bags. STUDY DESIGN AND METHODS: RBCs were sampled from blood bags and segments every two weeks during 8 weeks of storage at 4 degrees C. RBC deformability was measured by deformability-based sorting using the microfluidic ratchet device in order to derive a rigidity score. Standard hematological parameters, including mean corpuscular volume (MCV), red cell distribution width (RDW), mean cell hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and hemolysis were measured at the same time points. RESULTS: Deformability of RBCs stored in blood bags was retained over 4 weeks storage but a progressive loss of deformability was observed at weeks 6 and 8. This trend was mirrored in blood unit segments with a strong correlation to the blood bag data. Strong correlations were also observed between blood bag and segment for MCV, MCHC and MCH, but not for hemolysis. CONCLUSION: RBCs sampled from blood unit segments accurately represents the biophysical properties of RBCs in blood bags, but not hemolysis. Blood unit segments provide a simple and non-destructive sample for measuring RBC biophysical properties in clinical studies.

scholarly journals Influence of seasonality and pollution on the hematological parameters of the estuarine fish Centropomus parallelus

2013 ◽  
Vol 61 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Robson Seriani ◽  
Denis Moledo de Souza Abessa ◽  
Camilo D. S. Pereira ◽  
Aline A. Kirschbaum ◽  
Andrea Assunção ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
Hematological Parameters ◽  
Absolute Number ◽  
Aquatic Pollution ◽  
Estuarine Fish ◽  
Corpuscular Volume ◽  
Tropical Estuarine ◽  
Non Destructive

This study investigated the hematological parameters of the tropical estuarine fish Centropomus parallelus and their use as a non-destructive biomarker for aquatic pollution. Individuals were collected, in summer and winter, at two estuaries, Cananéia (CAN) and São Vicente (SVE), and blood was extracted by caudal puncture. The evaluated parameters were hematocrit (Ht), red blood cells (RBC), Mean Corpuscular Volume (MCV), and the leukocyte (WBC) and thrombocyte counts. Fishes from CAN showed higher values of absolute number of thrombocytes in the summer. The fishes from SVE presented lower values of Ht and MCV in winter. Comparing the hematological parameters of fishes from these two sites, Ht, MCV, WBC and RBC were higher in fishes from SVE in the summer, whereas during the winter, Ht and thrombocytes were higher in animals from SVE. The results allow attributing the changes in the blood of fishes to seasonality and the presence of contaminants.

scholarly journals Investigation of hematological parameters related to the severity of COVID-19 disease in Mashhad, Iran

2020 ◽  
Vol 7 (2) ◽  
pp. e19-e19
Author(s):  
Mohsen Seddigh-Shamsi ◽  
Shima Nabavi Mahali ◽  
Mahnaz Mozdourian ◽  
Abolghasem Allahyari ◽  
Neda Saeedian ◽  
...  
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Polymerase Chain Reaction Test ◽  
Healthcare Personnel ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Volume Number ◽  
Time Of Admission

Introduction:Human coronavirus disease 2019 (COVID-19) is an emerging respiratory disease that has become pandemic worldwide. The patients have a range of mild to severe symptoms, such as pneumonia, acute respiratory distress syndrome, arrhythmia, shock, and other organs involvement. Clinical and hematological evidence can serve as a prognostic factor to detect severe cases of COVID-19. Objectives: The present study aimed to evaluate hematological parameters related to the severity of the disease in COVID-19 patients in Mashhad, Eastern Iran. Patients and Methods: All the individuals with positive real-time reverse transcription-polymerase chain reaction test results and the patients with clinical symptoms whose computed tomography scan results were matched with the diagnosis of viral pneumonia were included in the study. The hematological examinations and complete blood count of the patients were performed by the healthcare personnel through collecting blood samples at the time of admission and discharge from the hospital. Results: In total, 189 COVID-19 patients were included in the study, 47.6% and 58.73% of whom were over 60 years old and male, respectively. Investigation of the hematological parameters revealed that the number of white blood cells, mean value of corpuscular volume, number of platelets, and lymph count had significantly increased at the time of discharge (P<0.01). On the other hand, red blood cells, hemoglobin, hematocrit, mean corpuscular hemoglobin, mean corpuscular hemoglobin, and red cell distribution width values were significantly higher at the time of admission in comparison to discharge (P<0.01). Furthermore, it was found that male gender had a significant relationship with the development of lymphopenia in the patients (P=0.021). Conclusion: It can be concluded that hematological parameters and inflammatory biomarkers in COVID-19 patients could potentially act as the independent factors and affect the prognosis of the disease.

scholarly journals Evidence for structural protein damage and membrane lipid remodeling in red blood cells from COVID-19 patients

2020 ◽  
Author(s):  
Tiffany Thomas ◽  
Davide Stefanoni ◽  
Monika Dzieciatkowska ◽  
Aaron Issaian ◽  
Travis Nemkov ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Saturated Fatty Acids ◽  
High Energy ◽  
Band 3 ◽  
Mean Corpuscular Hemoglobin ◽  
List Type ◽  
Structural Protein ◽  
The Impact

AbstractThe SARS-CoV-2 beta coronavirus is the etiological driver of COVID-19 disease, which is primarily characterized by shortness of breath, persistent dry cough, and fever. Because they transport oxygen, red blood cells (RBCs) may play a role in the severity of hypoxemia in COVID-19 patients.The present study combines state-of-the-art metabolomics, proteomics, and lipidomics approaches to investigate the impact of COVID-19 on RBCs from 23 healthy subjects and 29 molecularly-diagnosed COVID-19 patients. RBCs from COVID-19 patients had increased levels of glycolytic intermediates, accompanied by oxidation and fragmentation of ankyrin, spectrin beta, and the N-terminal cytosolic domain of band 3 (AE1). Significantly altered lipid metabolism was also observed, especially short and medium chain saturated fatty acids, acyl-carnitines, and sphingolipids. Nonetheless, there were no alterations of clinical hematological parameters, such as RBC count, hematocrit, and mean corpuscular hemoglobin concentration, with only minor increases in mean corpuscular volume. Taken together, these results suggest a significant impact of SARS-CoV-2 infection on RBC structural membrane homeostasis at the protein and lipid levels. Increases in RBC glycolytic metabolites are consistent with a theoretically improved capacity of hemoglobin to off-load oxygen as a function of allosteric modulation by high-energy phosphate compounds, perhaps to counteract COVID-19-induced hypoxia. Conversely, because the N-terminus of AE1 stabilizes deoxyhemoglobin and finely tunes oxygen off-loading, RBCs from COVID-19 patients may be incapable of responding to environmental variations in hemoglobin oxygen saturation when traveling from the lungs to peripheral capillaries and, as such, may have a compromised capacity to transport and deliver oxygen.Graphical AbstractKey PointsCOVID-19 promotes oxidation and fragmentation of membrane proteins, including the N-term of band 3RBCs from COVID-19 patients are characterized by increases in glycolysis and altered lipidomesCOVID-19 impacts two critical mechanisms that finely tune red cell membranes and hemoglobin oxygen affinity

Hematological changes and resistance of erythrocytes of crimean horses in response to 32 km races

2016 ◽  
Vol 3 (1) ◽  
pp. 66-72
Author(s):  
A. Andriichuk ◽  
H. Tkachenko ◽  
I. Tkachova
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Haemoglobin Concentration ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Red Cell ◽  
Cell Distribution ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Distribution Width

Aim. The aim of the present study was to investigate the alterations of some hematological parameters (hae- matocrit (HCT), haemoglobin concentration (HGB), the count of red blood cells (RBC), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW), as well as resistance of erythrocytes to urea and hydrogen peroxide in horses after 32 km endurance race. Methods. Seven horses from Crimea region (Bilohirsk, Crimean region) were involved in this study. Haematological parameters (haematocrit (HCT), haemoglobin concentration (HGB), the count of red blood cells (RBC), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW)) were determined with use of hematological and biochemical methods. Blood samples have been investigated by centrifugation at 3,000 g for 15 min. The peroxide and osmotic resistance of erythrocytes were determined spectrophoto- metrically at 540 nm by monitoring the rate of erythrocytes disintegration by hydrogen peroxide. Endurance h orses used in this study are trained and conditioned to perform over long distances at moderate speeds. The prolonged exercises were used in endurance race. The walk about 3 km/h for 20 min, the trot about 7 km/h for 15 min, and the canter about 5 km/h for 15 min) and the walk about 1 km were repeated for 1 h (phase I); rest in an outdoor paddock without access to water for 30 min. And phase II: the walk about 3 km/h for 20 min, the trot about 7 km/h for 15 min, and the canter about 5 km/h for 15 min) and the walk about 1 km was repeated for 1 h. Results. The results of the present study showed that adequate endurance race of low intensity could im- prove oxygen-dependent respiratory function in horses from Crimean region. Furthermore, the non-signifi cant increase of red blood cells indices in endurance horses indicate about good athletic level after 32 km endur- ance ride. Statistically signifi cant differences in the percentage of hemolyzed erythrocytes between pre- and post-ride period were observed and thereby signifying an oxidative stress-dependent impairment of erythrocyte stability. Conclusions. The haematological changes caused by various physical efforts refl ect changes in the functions of different systems and can be used for health control and diagnosis of diseases. It also allow the evaluating the level of sport performance, the accuracy of training, and physiological condition of horses.

scholarly journals Blood unit segments accurately represent the biophysical properties of red blood cells in blood bags but not hemolysis

Transfusion ◽  
2021 ◽  
Author(s):  
Emel Islamzada ◽  
Kerryn Matthews ◽  
Erik Lamoureux ◽  
Simon P. Duffy ◽  
Mark D. Scott ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Biophysical Properties ◽  
Blood Unit

scholarly journals Relationship Between Long-Term Beetroot Juice Supplementation and Hematological Parameters in Elite Fencers - a Pilot Study

2020 ◽  
Vol 12 (13) ◽  
pp. 73
Author(s):  
Olga Mizera ◽  
Lucyna Kozlowska ◽  
Ewelina Hallmann ◽  
Jolanta Gromadzinska ◽  
Anna Mroz ◽  
...  
Keyword(s):  
Platelet Count ◽  
Red Blood Cells ◽  
Physical Performance ◽  
Blood Cells ◽  
Mean Platelet Volume ◽  
Hematological Parameters ◽  
Beetroot Juice ◽  
Platelet Volume ◽  
Distribution Width

OBJECTIVE: This study aimed to analyze the long-term (4 weeks) effect of a diet and beetroot juice supplementation on hematological parameters, glutathione peroxidase activity in erythrocytes (GPx), and physical performance in elite fencers. METHODS: The study included 20 fencers and was conducted during the preparatory phase. Fencers underwent the fitness VO2max test at baseline - (B) and after two stages of implementation of the dietary recommendations &ndash; the first 4 weeks without beetroot juice (D) and the second with 26 g/d of freeze dried beetroot juice supplementation (D&amp;J). At B and after D and D&amp;J fasting blood samples were collected. RESULTS: After D and D&amp;J activities of GPx were significantly higher than those in B (p &lt; 0.000, p = 0.005 &ndash; respectively). After D&amp;J versus D significant increased red blood cells count (p = 0.038) and hemoglobin (p = 0.029), as well as decreased platelet count (p = 0.007), were observed. Additionally, after D&amp;J versus B a higher level of mean platelet volume (p = 0.043), energy (p = 0.001), and carbohydrate intake (p &lt; 0.000) were observed and a lower level of red cell distribution width (p = 0.006). CONCLUSION: Our findings provide evidence that long-time consumption of beetroot juice may improve some hematological parameters (red blood cells count, hemoglobin, platelet count, mean platelet volume) - one of the key elements of physical performance. However, seems to be that this effect is largely determined by an appropriate level of energy and nutrients intake.

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

scholarly journals Quantitative analysis of three-dimensional morphology and membrane dynamics of red blood cells during temperature elevation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Keyvan Jaferzadeh ◽  
MinWoo Sim ◽  
NamGon Kim ◽  
InKyu Moon
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Three Dimensional ◽  
Membrane Dynamics ◽  
Mean Corpuscular Hemoglobin ◽  
Rbc Membrane ◽  
Membrane Fluctuations ◽  
Highly Correlated ◽  
3D Profile ◽  
Shape Changes

Abstract The optimal functionality of red blood cells is closely associated with the surrounding environment. This study was undertaken to analyze the changes in membrane profile, mean corpuscular hemoglobin (MCH), and cell membrane fluctuations (CMF) of healthy red blood cells (RBC) at varying temperatures. The temperature was elevated from 17 °C to 41 °C within a duration of less than one hour, and the holograms were recorded by an off-axis configuration. After hologram reconstruction, we extracted single RBCs and evaluated their morphologically related features (projected surface area and sphericity coefficient), MCH, and CMF. We observed that elevating the temperature results in changes in the three-dimensional (3D) profile. Since CMF amplitude is highly correlated to the bending curvature of RBC membrane, temperature-induced shape changes can alter CMF’s map and amplitude; mainly larger fluctuations appear on dimple area at a higher temperature. Regardless of the shape changes, no alterations in MCH were seen with temperature variation.

scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

Immunoregulatory Effects of Stored Red Blood Cells

Hematology ◽  
2011 ◽  
Vol 2011 (1) ◽  
pp. 466-469 ◽  
Author(s):  
Karina Yazdanbakhsh ◽  
Weili Bao ◽  
Hui Zhong
Keyword(s):  
Red Blood Cells ◽  
Patient Outcomes ◽  
Clinical Studies ◽  
Blood Cells ◽  
Immunomodulatory Effects ◽  
Tissue Microenvironment ◽  
High Concentrations ◽  
Transfusion Reactions ◽  
Immunoregulatory Mechanisms

Abstract Some clinical studies have identified potential adverse patient outcomes associated with RBC storage length. This may in part be due to the release of potentially hazardous bioactive products that accumulate during storage and are delivered at high concentrations during transfusion. In this situation, a proinflammatory tissue microenvironment may be established that can alter immunoregulatory mechanisms. This review highlights some of the potential immunomodulatory effects of stored RBCs that may be responsible for adverse transfusion reactions.

Sign in / Sign up

Export Citation Format

Share Document