scholarly journals Inactivation effect and damage of multi-irradiance by UVCLED on Acinetobacter baumannii

2021 ◽  
Author(s):  
Mengmeng Li ◽  
Baiqin Zhao ◽  
Lei Han ◽  
Zhen Wang
Keyword(s):  
Dna Damage ◽  
Drug Resistance ◽  
Cell Wall ◽  
Gel Electrophoresis ◽  
Cellular Membrane ◽  
High Irradiance ◽  
Transmission Electron ◽  
Ultraviolet C ◽  
The Relationship ◽  
Inactivation Effect

It is acknowledged that the inactivation of ultraviolet has been widely used in various fields. Much literature has been reported that ultraviolet C caused DNA damage to achieve inactivation of microorganisms. There is a lack of unified dose calibration and related parameters in this field. In this study, we used a device consisted of the LED of 272 nm to conduct sterilization experiments against A. baumanii. We confirmed the effectiveness of ultraviolet C sterilization for both sensitive and drug resistance strains and explored the relationship between bactericidal rate and ultraviolet doses under various irradiance. Dose requirements of various irradiance were clarified. High irradiance improved sterilization efficiency greatly. The overall damage to the total genome was observed though gel electrophoresis. Ultrastructures of damaged bacteria were investigated by transmission electron microscope in detail. The study revealed that damage to DNA and to the cytoplasm matrix and ribosomes. The study has yielded the possible effects of ultraviolet light on cells by amplifying the energy. The radiation significantly promoted the production of cell wall and cellular membrane.

Acid and alkaline phosphatases of Capnocytophaga species. III. The relationship of the enzymes to the cell wall

1983 ◽  
Vol 29 (10) ◽  
pp. 1369-1381 ◽  
Author(s):  
Thomas P. Poirier ◽  
Stanley C. Holt
Keyword(s):  
Cell Wall ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Marker Enzyme ◽  
Transmission Electron ◽  
Sds Page ◽  
Triton X ◽  
Relationship Of ◽  
The Relationship ◽  
Cell Fragments

Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9–28% and 11–43% of the cellular phosphatases, respectively. French pressure cell disruption was more effective in releasing the phosphatases. Cell fragments were separated into cell wall, cytoplasmic membrane, and soluble fractions as determined by marker enzyme, chemical composition, transmission electron microscopy, sodium dodecyl sulphate – polyacrylamide gel electrophoresis (SDS–PAGE) protein analysis, and gel diffusion. AcP and AlP was partitioned between the isolated cell wall (31–46%) and soluble material (33–61%), with greater than 60% of the phosphatases remaining with the cell wall following Triton X-100 treatment. The amount of phosphatase at the surface of intact C. ochracea was quantitated by specific 125I-protein labelling.

Endocytosis in elongating root cells of Lobelia erinus

1990 ◽  
Vol 97 (1) ◽  
pp. 157-165
Author(s):  
A. L. SAMUELS ◽  
T. BISALPUTRA
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Multivesicular Bodies ◽  
Secretory Vesicles ◽  
Membrane Material ◽  
Root Cells ◽  
Transmission Electron ◽  
Dense Deposits ◽  
The Relationship ◽  
Conventional Transmission Electron Microscope

Endocytosis was demonstrated in elongating cortical and epidermal root cells of Lobelia erinus using the apoplast marker lanthanum nitrate. Lanthanum treatment produced electron-dense deposits throughout the cell wall, as well as in coated and smooth vesicles, partially coated reticula, and multivesicular bodies. This labelling pattern was observed in root cells that had been ultrarapidly frozen on a copper mirror and freeze-substituted (cryofixation) or fixed by conventional transmission electron microscope (TEM) techniques. The amount of endocytosis occurring was measured by counting the number of vesicles μm−2 in root cells at various stages of development. Endocytosis occurred most in actively elongating cells, and least in mature cells, which were no longer elongating. The relationship between endocytosis and active cell wall secretion suggests that endocytosis may be acting to remove excess plasma membrane material added during exocytosis of secretory vesicles.

Influence of Cell Wall Composition on the Fossilisation of Bacteria and the Implications for the Search for Early Life Forms

1997 ◽  
Vol 161 ◽  
pp. 491-504 ◽  
Author(s):  
Frances Westall
Keyword(s):  
Cell Wall ◽  
Life Forms ◽  
Cation Binding ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Hydrothermal Sediments ◽  
Identification Of Bacteria ◽  
The Difference

AbstractThe oldest cell-like structures on Earth are preserved in silicified lagoonal, shallow sea or hydrothermal sediments, such as some Archean formations in Western Australia and South Africa. Previous studies concentrated on the search for organic fossils in Archean rocks. Observations of silicified bacteria (as silica minerals) are scarce for both the Precambrian and the Phanerozoic, but reports of mineral bacteria finds, in general, are increasing. The problems associated with the identification of authentic fossil bacteria and, if possible, closer identification of bacteria type can, in part, be overcome by experimental fossilisation studies. These have shown that not all bacteria fossilise in the same way and, indeed, some seem to be very resistent to fossilisation. This paper deals with a transmission electron microscope investigation of the silicification of four species of bacteria commonly found in the environment. The Gram positiveBacillus laterosporusand its spore produced a robust, durable crust upon silicification, whereas the Gram negativePseudomonas fluorescens, Ps. vesicularis, andPs. acidovoranspresented delicately preserved walls. The greater amount of peptidoglycan, containing abundant metal cation binding sites, in the cell wall of the Gram positive bacterium, probably accounts for the difference in the mode of fossilisation. The Gram positive bacteria are, therefore, probably most likely to be preserved in the terrestrial and extraterrestrial rock record.

Molecular Structure of the Outermost Cell Wall Component of Spirillum Serpens

1977 ◽  
Vol 35 ◽  
pp. 342-343
Author(s):  
Wah Chiu ◽  
David Grano
Keyword(s):  
Molecular Structure ◽  
Cell Wall ◽  
Outer Membrane ◽  
Gel Electrophoresis ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Cell Wall Component ◽  
Protein Components ◽  
Exposure Technique ◽  
Structural Aspects

The periodic structure external to the outer membrane of Spirillum serpens VHA has been isolated by similar procedures to those used by Buckmire and Murray (1). From SDS gel electrophoresis, we have found that the isolated fragments contain several protein components, and that the crystalline structure is composed of a glycoprotein component with a molecular weight of ∽ 140,000 daltons (2). Under an electron microscopic examination, we have visualized the hexagonally-packed glycoprotein subunits, as well as the bilayer profile of the outer membrane. In this paper, we will discuss some structural aspects of the crystalline glycoproteins, based on computer-reconstructed images of the external cell wall fragments.The specimens were prepared for electron microscopy in two ways: negatively stained with 1% PTA, and maintained in a frozen-hydrated state (3). The micrographs were taken with a JEM-100B electron microscope with a field emission gun. The minimum exposure technique was essential for imaging the frozen- hydrated specimens.

Ultrastructure of commercial recycled pulp fibers for the production of packaging paper

Holzforschung ◽  
2005 ◽  
Vol 59 (6) ◽  
pp. 675-680 ◽  
Author(s):  
Jonas Brändström ◽  
Jean-Paul Joseleau ◽  
Alain Cochaux ◽  
Nathalie Giraud-Telme ◽  
Katia Ruel
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Significant Proportion ◽  
Pulp Fibers ◽  
Chemical Pulp ◽  
Recycled Pulp ◽  
Transmission Electron ◽  
Large Heterogeneity ◽  
Recycled Fibers ◽  
Packaging Paper

Abstract Transmission electron microscopy was used to investigate the ultrastructure of recycled pulp fibers originating from a household collection plant and intended for the production of packaging paper. Three recovered paper grades and recycling processes, including pulping, screening, cleaning and refining, were assessed with emphasis on surface and internal fibrillation as well as xylan localization. Results showed a large heterogeneity with respect to fiber ultrastructure within and between the grades. Screening and cleaning steps had no detectable effects, but refining clearly increased cell-wall delamination and surface fibrillation. Immunolabeling of xylans showed that they were distributed rather evenly across the cell walls. They were also present on fines. Two different mechanisms for fiber delamination and surface fibrillation were found, one which implies that internal and external fibrillation take place simultaneously across the cell wall, and another which implies successive peeling of layers or sub-layers from the outside towards the inside. It is suggested that recycled fibers of chemical pulp origin undergo the former mechanism and recycled fibers that contain lignin binding the cell wall matrix give rise to the latter peeling mechanism. Because several recycled fibers were severely delaminated and almost fractured, we suggest that to produce a good packaging paper, it is important that recycled pulp should contain a significant proportion of fibers with high intrinsic strength.

scholarly journals Chemical Quantification of Atomic-Scale EDS Maps under Thin Specimen Conditions

2014 ◽  
Vol 20 (6) ◽  
pp. 1782-1790 ◽  
Author(s):  
Ping Lu ◽  
Eric Romero ◽  
Shinbuhm Lee ◽  
Judith L. MacManus-Driscoll ◽  
Quanxi Jia
Keyword(s):  
Atomic Scale ◽  
Specimen Thickness ◽  
Peak Width ◽  
Thin Specimen ◽  
Gaussian Peak ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
The Relationship ◽  
Chemical Quantification

AbstractWe report our effort to quantify atomic-scale chemical maps obtained by collecting energy-dispersive X-ray spectra (EDS) using scanning transmission electron microscopy (STEM) (STEM-EDS). With thin specimen conditions and localized EDS scattering potential, the X-ray counts from atomic columns can be properly counted by fitting Gaussian peaks at the atomic columns, and can then be used for site-by-site chemical quantification. The effects of specimen thickness and X-ray energy on the Gaussian peak width are investigated using SrTiO3 (STO) as a model specimen. The relationship between the peak width and spatial resolution of an EDS map is also studied. Furthermore, the method developed by this work is applied to study cation occupancy in a Sm-doped STO thin film and antiphase boundaries (APBs) present within the STO film. We find that Sm atoms occupy both Sr and Ti sites but preferably the Sr sites, and Sm atoms are relatively depleted at the APBs likely owing to the effect of strain.

Haustorial development of Peronospora tabacina infecting Nicotiana tabacum

1983 ◽  
Vol 61 (12) ◽  
pp. 3444-3453 ◽  
Author(s):  
R. N. Trigiano ◽  
C. G. Van Dyke ◽  
H. W. Spurr Jr.
Keyword(s):  
Cell Wall ◽  
Toluidine Blue ◽  
Mesophyll Cell ◽  
Wall Layer ◽  
Peronospora Tabacina ◽  
Host Cytoplasm ◽  
Transmission Electron ◽  
Mold Fungus ◽  
Host Cell Wall ◽  
Hyphal Wall

The development of haustoria in tobacco by the blue-mold fungus Peronospora tabacina was examined using light, scanning, and transmission electron microscopy. Electron-lucent, callose-like appositions were observed between the host plasmalemma and the host mesophyll cell wall prior to haustorial penetration. An electron-opaque penetration matrix was present between the apposition and the host cell wall. The intercellular hyphal wall consisted of two layers which differed in staining quality. The haustorial wall was also two layered, but was primarily composed of and continuous with the inner wall layer of the intercellular hypha. Haustoria were either finger-like or branched and were encased with callose-like material. Most encasements were thickened at the proximal regions of haustoria but were thinner along the distal portions. Vesicles were present in host cytoplasm and were occasionally attached to the invaginated host plasmalemma. These vesicles might contribute to the deposition of the encasement material. The encasement stained positively for callose using aniline blue; calcofluor and toluidine blue O tests for cellulose were inconclusive, and lignin was not detected using toluidine blue O or phloroglucinol–HCl.

scholarly journals Minimal, superficial DNA damage in human skin from filtered far‐ultraviolet‐C (UV‐C)

2021 ◽  
Author(s):  
R.P. Hickerson ◽  
M.J. Conneely ◽  
S.K. Hirata Tsutsumi ◽  
K. Wood ◽  
D.N. Jackson ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Skin ◽  
Ultraviolet C ◽  
Far Ultraviolet ◽  
Uv C

Interfaces in Structural Ceramics

MRS Bulletin ◽  
1990 ◽  
Vol 15 (10) ◽  
pp. 51-59 ◽  
Author(s):  
M. Grant Norton ◽  
C. Barry Carter
Keyword(s):  
Structural Ceramics ◽  
Interface Engineering ◽  
Micro Structure ◽  
Complete Understanding ◽  
Intimate Contact ◽  
Transmission Electron ◽  
Processed Material ◽  
Wide Range ◽  
Alternative Materials ◽  
The Relationship

Structural ceramics are necessarily polycrystalline and their usefulness is largely determined by the interfaces between the grains. The relationship between the structure and chemistry of different interfaces and the micro-structure can be illustrated by reviewing studies of interfaces in a wide range of materials including such classical ceramics as Al2O3, the current “hightech” polyphase ceramics exemplified by ZrO2-toughened Al2O3, and the composite materials of the future. Using transmission electron microscopy is essential for a complete understanding, but limitations to its use must be recognized. Only by understanding the factors that control the behavior of these interfaces will it become possible to further extend the application of interface engineering.Structural ceramics are a group of materials that can be used for applications requiring their strength to persist at high temperatures or in conditions that would be particularly corrosive to alternative materials, which are usually metallic. Strength and strength-related properties such as toughness depend largely on the microstructural features of the processed material.The microstructure is defined by the morphology and size of the grains and the interfaces between these grains. If the grains are in intimate contact, then the interface is a grain boundary of the type familiar from studies of metals.

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