scholarly journals High-speed device synchronization in optical microscopy with an open-source hardware control platform

2019 ◽  
Author(s):  
Marshall J. Colville ◽  
Sangwoo Park ◽  
Warren R. Zipfel ◽  
Matthew J. Paszek
Keyword(s):  
Open Source ◽  
High Speed ◽  
Interference Microscopy ◽  
Scanning Angle ◽  
Precise Control ◽  
Synchronization Errors ◽  
Waveform Generation ◽  
High Speeds ◽  
Control And Synchronization ◽  
Open Source Hardware

AbstractRecent advances in fluorescence microscopy have enabled the visualization of subcellular structures at unprecedented resolution. However, the complexity of state-of-the-art microscopes has increased considerably, often requiring the precise control and synchronization of multiple peripheral devices at high speeds. Drawing inspiration from open-source prototyping systems, like the Arduino, we describe the development of a new control platform that adopts the best features of these systems – affordability, facile programmability, and flexible connectivity – but with the scientific-grade inputs and outputs (I/O) and built-in routines that are necessary to control peripherals in advanced microscopy applications. Notably, our platform includes waveform generators and I/O for point-and azimuthal-scanning of excitation in laser-based applications. As a proof of concept, we show how the integration of waveform generation, multiplexed analog outputs, and native hardware triggers into a single central hub provides a versatile platform for performing fast circle-scanning acquisitions, including ring scanning-angle interference microscopy (SAIM), total internal reflection fluorescence (TIRF and ring TIRF) microscopy, and multiangle TIRF (MA-TIRF). We also demonstrate how the low communication latency of our hardware platform can reduce image intensity and reconstruction artifacts arising from synchronization errors produced by software control. Our complete platform, including hardware design files, firmware, API, software, and all associated source code, will be hosted for community-based development and collaboration.

scholarly journals High-speed device synchronization in optical microscopy with an open-source hardware control platform

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Marshall J. Colville ◽  
Sangwoo Park ◽  
Warren R. Zipfel ◽  
Matthew J. Paszek
Keyword(s):  
Optical Microscopy ◽  
Open Source ◽  
High Speed ◽  
Open Source Hardware

Numerical Analysis of Rotor/Propeller Aerodynamic Interactions on a High-Speed Compound Helicopter

2021 ◽  
Author(s):  
Ronan Boisard
Keyword(s):  
High Speed ◽  
Strong Interactions ◽  
Main Rotor ◽  
Rotor Wake ◽  
Precise Control ◽  
High Speeds ◽  
Computational Fluid Dynamics Cfd ◽  
Compound Helicopter ◽  
Wake Model ◽  
Free Wake

In the context of the development of high-speed compound helicopters, the main rotor may not be an efficient propulsive device at high speeds and adding a propulsive propeller is a means to enable higher speed. On such configuration, at low speed, the propellers are in strong interactions with the main rotor wake which affects their performance and aircraft maneuverability. The present work numerically investigates the aerodynamics of the rotor/propeller interaction on rotorcraft similar to the Racer from Airbus Helicopters. Through the comparison of two different levels of fidelity for three different advance ratios, it is shown that at high advance ratio, a simple free wake model is suitable to give most of the interaction effects, while in hover, a full computational fluid dynamics (CFD) unsteady computation is necessary to better capture all the unsteadiness of the interaction. The detailed analysis of CFD results also outlines the different behaviors of the propeller when it is fully inside the rotor wake or out of it, and therefore the need for a precise control of the rotorcraft in the transition between hover to fast forward flight.

scholarly journals A data acquisition and analysis pipeline for scanning angle interference microscopy

2016 ◽  
Author(s):  
Catherine B. Carbone ◽  
Ronald D. Vale ◽  
Nico Stuurman
Keyword(s):  
Data Acquisition ◽  
Open Source ◽  
Open Source Software ◽  
Interference Microscopy ◽  
Analysis Pipeline ◽  
Scanning Angle ◽  
Software And Hardware

AbstractWe describe open source software and hardware tools for calibrating, acquiring, and analyzing images for scanning angle interference microscopy (SAIM) analysis. These tools make it possible for any user with a TIRF microscope equipped with a motorized illumination unit to generate reconstructed images with nanometer precision in the axial (z) direction and diffraction-limited resolution in the lateral (xy) plane.

A Statistical Study of Process Variables to Optimize a High Speed Curtain Coater: Part I

TAPPI Journal ◽  
2009 ◽  
Vol 8 (1) ◽  
pp. 20-26 ◽  
Author(s):  
PEEYUSH TRIPATHI ◽  
MARGARET JOYCE ◽  
PAUL D. FLEMING ◽  
MASAHIRO SUGIHARA
Keyword(s):  
Boundary Layer ◽  
Experimental Design ◽  
High Speed ◽  
Statistical Study ◽  
Process Variables ◽  
High Speeds ◽  
The Stability ◽  
Air Removal ◽  
Removal System

Using an experimental design approach, researchers altered process parameters and material prop-erties to stabilize the curtain of a pilot curtain coater at high speeds. Part I of this paper identifies the four significant variables that influence curtain stability. The boundary layer air removal system was critical to the stability of the curtain and base sheet roughness was found to be very important. A shear thinning coating rheology and higher curtain heights improved the curtain stability at high speeds. The sizing of the base sheet affected coverage and cur-tain stability because of its effect on base sheet wettability. The role of surfactant was inconclusive. Part II of this paper will report on further optimization of curtain stability with these four variables using a D-optimal partial-facto-rial design.

ON METHODS OF OBJECT DETECTION IN VIDEO STREAMS

2017 ◽  
Vol 2 (1) ◽  
pp. 80-87
Author(s):  
Puyda V. ◽  
◽  
Stoian. A.
Keyword(s):  
Computer Vision ◽  
Object Detection ◽  
Open Source ◽  
Feature Detection ◽  
Video Stream ◽  
Object Identification ◽  
Vision Systems ◽  
Modern Computer ◽  
Computer Vision Systems ◽  
Open Source Hardware

Detecting objects in a video stream is a typical problem in modern computer vision systems that are used in multiple areas. Object detection can be done on both static images and on frames of a video stream. Essentially, object detection means finding color and intensity non-uniformities which can be treated as physical objects. Beside that, the operations of finding coordinates, size and other characteristics of these non-uniformities that can be used to solve other computer vision related problems like object identification can be executed. In this paper, we study three algorithms which can be used to detect objects of different nature and are based on different approaches: detection of color non-uniformities, frame difference and feature detection. As the input data, we use a video stream which is obtained from a video camera or from an mp4 video file. Simulations and testing of the algoritms were done on a universal computer based on an open-source hardware, built on the Broadcom BCM2711, quad-core Cortex-A72 (ARM v8) 64-bit SoC processor with frequency 1,5GHz. The software was created in Visual Studio 2019 using OpenCV 4 on Windows 10 and on a universal computer operated under Linux (Raspbian Buster OS) for an open-source hardware. In the paper, the methods under consideration are compared. The results of the paper can be used in research and development of modern computer vision systems used for different purposes. Keywords: object detection, feature points, keypoints, ORB detector, computer vision, motion detection, HSV model color

RED CUT COBALT

Alloy Digest ◽  
1980 ◽  
Vol 29 (8) ◽  
Keyword(s):  
Fracture Toughness ◽  
Cast Iron ◽  
High Speed ◽  
Elevated Temperatures ◽  
High Speed Steel ◽  
Heat Treating ◽  
High Speeds ◽  
Red Hardness ◽  
Nonferrous Alloys ◽  
Cutting Point

Abstract RED CUT COBALT steel is made by adding 5% cobalt to the conventional 18% tungsten -4% chromium-1% vanadium high-speed steel. Cobalt increases hot or red hardness and thus enables the tool to maintain a higher hardness at elevated temperatures. This steel is best adapted for hogging cuts or where the temperature of the cutting point of the tool in increased greatly. It is well adapted for tools to be used for reaming cast-iron engine cylinders, turning alloy steel or cast iron and cutting nonferrous alloys at high speeds. This datasheet provides information on composition, physical properties, and hardness as well as fracture toughness. It also includes information on forming, heat treating, and machining. Filing Code: TS-367. Producer or source: Teledyne Vasco.

CPM REX 25

Alloy Digest ◽  
1980 ◽  
Vol 29 (7) ◽  
Keyword(s):  
Heat Treatment ◽  
High Speed ◽  
High Speed Steel ◽  
High Hardness ◽  
Heat Treating ◽  
High Speeds ◽  
Aisi Type ◽  
Tool Performance ◽  
End Mills ◽  
Machining Operations

Abstract CPM REX 25 is a super high-speed steel made without cobalt. It is comparable to AISI Type T15 cobalt-containing high-speed steel in response to heat treatment, properties, and tool performance. CPM REX 25 is recommended for machining operations requiring heavy cuts, high speeds and feeds, and difficult-to-machine materials of high hardness and abrasion resistance. Typical applications are boring tools, drills, gear cutters, punches, form tools, end mills and broaches. This datasheet provides information on composition, physical properties, hardness, and elasticity as well as fracture toughness. It also includes information on forming, heat treating, machining, and surface treatment. Filing Code: TS-365. Producer or source: Crucible Materials Corporation.

scholarly journals ccNetViz: a WebGL-based JavaScript library for visualization of large networks

2020 ◽  
Vol 36 (16) ◽  
pp. 4527-4529
Author(s):  
Ales Saska ◽  
David Tichy ◽  
Robert Moore ◽  
Achilles Rasquinha ◽  
Caner Akdas ◽  
...  
Keyword(s):  
Systems Biology ◽  
Complex Networks ◽  
Open Source ◽  
High Speed ◽  
A Priori ◽  
Supplementary Information ◽  
Network Visualization ◽  
Supplementary Data ◽  
Web Based ◽  
Flow Of Information

Abstract Summary Visualizing a network provides a concise and practical understanding of the information it represents. Open-source web-based libraries help accelerate the creation of biologically based networks and their use. ccNetViz is an open-source, high speed and lightweight JavaScript library for visualization of large and complex networks. It implements customization and analytical features for easy network interpretation. These features include edge and node animations, which illustrate the flow of information through a network as well as node statistics. Properties can be defined a priori or dynamically imported from models and simulations. ccNetViz is thus a network visualization library particularly suited for systems biology. Availability and implementation The ccNetViz library, demos and documentation are freely available at http://helikarlab.github.io/ccNetViz/. Supplementary information Supplementary data are available at Bioinformatics online.

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