scholarly journals A survey of vaccine-induced measles IgG antibody titer and the verification of changes in temporal differences of measles vaccination in young adults

2019 ◽  
Author(s):  
Hiraku Sasaki ◽  
Tomoko Fukunaga ◽  
Ai Asano ◽  
Yoshio Suzuki ◽  
Yuko Nakanishi ◽  
...  
Keyword(s):  
Young Adult ◽  
Antibody Titer ◽  
Measles Virus ◽  
Serum Samples ◽  
Igg Antibody ◽  
Adult Participants ◽  
Significant Difference ◽  
Antibody Titers ◽  
Measles Vaccination ◽  
Specific Igg

AbstractIn Japan, sporadic measles cases increased rapidly in 2019 compared to that in past 6 years. To clarify the persistence of immunity against measles in young adult, this study explored the prevalence of IgG antibody titer against measles virus in 18-to 24-year-old young adult participants. Measles-specific IgG antibody titers determined by enzyme immunoassay in serum samples collected from 506 participants between 18 to 24 years were assessed through statistical analyses. Multiple regression analysis revealed that the distribution of measles IgG antibody titers was significantly correlated with medical history (P < 0.05), while there was no significant difference among the number of vaccinations related to measles IgG titers. Further, measles IgG titers were significantly different, which was determined by the temporal change that elapsed period after last vaccination (P < 0.05). These results indicate that periodic vaccination against measles is required for young and older adults to prevent even sporadic measles infection.

scholarly journals A Survey of Vaccine-Induced Measles IgG Antibody Titer to Verify Temporal Changes in Response to Measles Vaccination in Young Adults

Vaccines ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 118
Author(s):  
Hiraku Sasaki ◽  
Tomoko Fukunaga ◽  
Ai Asano ◽  
Yoshio Suzuki ◽  
Yuko Nakanishi ◽  
...  
Keyword(s):  
Young Adults ◽  
Measles Virus ◽  
Serum Samples ◽  
Igg Antibody ◽  
Chiba Prefecture ◽  
History Of ◽  
Antibody Titers ◽  
Multivariable Regression ◽  
Measles Vaccination ◽  
Specific Igg

In Japan, sporadic measles cases increased rapidly in 2019 compared to the past six years. To clarify the persistence of immunity against measles in young adults, this study explored the persistence of immunoglobulin G (IgG) antibody titers against the measles virus in 17- to 24-year-old young participants who reside in the Chiba prefecture of Japan. Measles-specific IgG antibody titers, determined by enzyme immunoassay in serum samples collected from 506 participants, were assessed through statistical analyses. Multivariable regression analysis revealed that the distribution of measles IgG antibody titers was significantly correlated with a medical history of measles (P < 0.05), while there was no significant correlation between the number of vaccinations related to measles IgG titers. Furthermore, measles IgG titers tended to decrease, as revealed by the temporal change in IgG titers, during the elapsed period after the last vaccination (P = 0.08). These results indicate that periodic vaccination against measles is required to prevent sporadic measles infection in young and older adults.

scholarly journals Detection of measles cases in the Republic of Guinea in 2017–2018

2020 ◽  
Vol 10 (3) ◽  
pp. 570-574
Author(s):  
I. N. Lavrentieva ◽  
M. A. Bichurina ◽  
A. Y. Antipova ◽  
J. Camara ◽  
N.F. Magassouba
Keyword(s):  
Antibody Level ◽  
Measles Virus ◽  
Age Groups ◽  
Cold Chain ◽  
Epidemiological Surveillance ◽  
Serum Samples ◽  
Igg Antibody ◽  
Republic Of Guinea ◽  
Measles Vaccination ◽  
The Republic

In 2017, WHO reported 596 confirmed measles cases in Guinea Republic connected to the 2016–2017 epidemic outbreak that was stopped after additional immunization (SIA) against measles in two provinces of the country. Improving the effectiveness of SIA is associated with the identification of epidemiologically significant groups of the population. The aim of the study was to analyze 2017–2018 measles cases and assess population immunity to measles virus in the Republic of Guinea. Materials and methods. A total of 810 blood serum samples collected from patients with maculo-papular rash and clinical diagnosis “measles?” were tested for measles virus-specific IgM-and IgG antibody level. 445 sera of conditionally healthy individuals aged 7 months to 67 years were examined for anti-measles virus IgG antibody level. Immunoglobulins of classes M and G were detected by ELISA with test systems «Anti-Measles Virus ELISA (IgM)» (Euro immun, Germany) and «Anti-Measles Virus ELISA (IgG)» (Euroimmun, Germany). Results and discussion.In 2017–2018, the epidemic process of the measles in the Republic of Guinea proceeded very intensively, being markedly prevalent in children among age groups. In 2018, more than half of the cases (61.6%) were identified in children aged 1 to 5 years old; the second most abundant age group was children under one year (18.6%), probably due to violated measles vaccination, which in GR are subject to children of nine months of age. It was found that 16.4% of patients (60 out of 366) had documented data on measles vaccination. Potentially, high proportion of measles cases among pre-vaccinated subjects was due to insufficient immune response to a single immunization in children of 9 months of age. Moreover, lowered vaccine-related properties might also be violated “cold chain” during vaccine transportation occurring in tropical climate. Analyzing 445 subjects revealed that total number of measles virus seronegative subjects was 8.3%. However, the vast majority of them were children and young adults aged 7 months to 22 years, where 52.4% of seronegative subjects were identified. Thus, the data obtained indicate that intensive measles virus circulation in human population was continued that necessitate interventions for improving epidemiological surveillance, extend routine measles vaccination coverage and conduct SIAs against measles in GR.

Comparison of Covid-19 IgG Anti-Spike Antibody Titer after Vaccination with Sputnik V in Seropositive and Naïve Vaccinees

2021 ◽  
Vol 35 (4) ◽  
pp. 1-5
Author(s):  
Hajra Farooq
Keyword(s):  
Single Dose ◽  
Antibody Titer ◽  
Immunological Response ◽  
Severe Infection ◽  
P Value ◽  
Igg Antibody ◽  
Policy Makers ◽  
Significant Difference ◽  
Antibody Titers ◽  
Study Participants

Introduction: Immunity to SARS-CoV-2 has shown to reduce the risk of having a severe infection and initiate a good degree of disease protection. Studies assessing the antibody titer after vaccination can be very helpful to see whether previously infected individuals have better immunological response as compared to uninfected or antibody naïve individuals. Aims & Objectives: Comparison of Anti-spike IgG antibody among vaccinees with or without previous exposure to COVID-19. To determine whether single dose regimen can produce significant antibody titer amongst previously infected cases and design vaccine dosage regimens accordingly. Place and duration of study: This study was conducted at Chughtai Institute of Pathology from April 2021 to June 2021. Material & Methods: Blood samples were collected from 83 adult male and female vaccinees at baseline, 3 weeks after the first dose and finally 7 days after the second dose. Previously infected individuals’ record was noted separately. Samples were immediately analyzed using Abbott SARS-CoV-2 IgG II quant two step immunoassay. Data was analyzed using SPSS 23.0. A p-value of <0.05 was considered significant.Results: Majority of the candidates (57 %) were females and on analysis it was found that 42% of the patients were seropositive whereas 58% of the patients were antibody naïve before receiving the first dose of vaccine. There was a significant difference between mean antibody titer of seropositive and seronegative study participants at day 0, day 21 and finally on day 28 (p value <0.001) with seropositive individuals having higher antibody titers even after first vaccine shot. Conclusion: Post vaccination immunological response was higher in seropositive individuals as compared to the antibody naïve and this finding can help the policy makers to design a single dose vaccine regimen for the former category.

scholarly journals Measles serodiagnosis: standardization and evaluation of a dot-ELISA

1994 ◽  
Vol 36 (2) ◽  
pp. 139-147 ◽  
Author(s):  
Lourdes R. A. Vaz de Lima ◽  
Sumie Hoshino-Shimizu ◽  
Vanda A. U. F. de Souza ◽  
Claudio S. Pannuti ◽  
Heitor F. Andrade Junior ◽  
...  
Keyword(s):  
Measles Virus ◽  
Sodium Deoxycholate ◽  
Antibody Detection ◽  
Serum Samples ◽  
Igg Antibody ◽  
Measles Vaccination ◽  
Fluorescence Test ◽  
The Stability ◽  
Dot Elisa

A Dot-ELISA using a measles virus (MV) antigen obtained by sodium deoxycholate treatment was standardized and evaluated for IgM and IgG antibody detection in measles patients and measles-vaccinated subjects. A total of 192 serum samples were studied, comprising 47 from patients with acute and convalescent measles, 55 from 9-month old children prior to measles vaccination and 41 from children of the same age after vaccination, and 49 from patients with unrelated diseases. The diagnostic performances of the IgG Dot-ELISA and IgG immuno fluorescence test (IFT) were found to be close, varying from 0.97 to 1.00 in sensitivity and the specificities were maximum (1.00). Nevertheless, the sensitivity of the IgM Dot-ELISA (0.85) was higher than that (0.63) of the IgM IFT, although both assays had comparably high (1.00) specificities. The IgM Dot-ELISA in particular proved to be more sensitive in relation to other assays studied by revealing antibodies in 80.0% (12/15) of vaccinated children on the 15th day after immunization. In contrast the IgM IFT, failed to detect antibodies in the same group of vaccinated children. The stability of the MV antigen was longer than that of the IFT antigen, and the reproducibility of the Dot-Elisa was satisfactory.

scholarly journals Longitudinal Analysis of Tetanus- and Influenza-Specific IgG Antibodies in Myeloma Patients

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Sebastian Kobold ◽  
Tim Luetkens ◽  
Britta Marlen Bartels ◽  
Yanran Cao ◽  
York Hildebrandt ◽  
...  
Keyword(s):  
Longitudinal Analysis ◽  
Clinical Status ◽  
Cell Mediated Immunity ◽  
Serum Samples ◽  
Igg Antibody ◽  
Specific Antibodies ◽  
Time Period ◽  
Healthy Donors ◽  
Antibody Titers ◽  
Specific Igg

Background. Multiple myeloma (MM) and its therapies may induce a severely compromised humoral immunity. We have performed a longitudinal analysis of IgG-antibody responses against influenza virus (FLU) and tetanus toxoid (TT) as surrogate markers for the B cell-mediated immunity in MM patients.Methods. 1094 serum samples of 190 MM patients and samples from 100 healthy donors were analyzed by ELISA for FLU- and TT-specific antibodies.Results. MM patients evidenced lower levels of FLU- and TT-specific antibodies than healthy controls (P<0.001). Immunoreactivity decreased with progressing disease and worsening clinical status. Levels of FLU- and TT-specific antibodies increased shortly (0-6 months) after alloSCT (P<0.001), a time-period during which intravenous immunoglobulin (IVIG) is routinely applied. Thereafter, antibody concentrations declined and remained suppressed for 3 years in the case of FLU-specific and for more than 5 years in the case of TT-specific antibodies.Conclusions. We found that MM is associated with a profound disease- and therapy-related immunosuppression, which is compensated for a few months after alloSCT, most likely by application of IVIG. This and the differences regarding the recovery of anti-FLU and anti-TT antibody titers during the following years need to be taken into account for optimizing IVIG application and immunization after alloSCT.

scholarly journals Longitudinal changes in IgG levels among COVID-19 recovered patients: A prospective cohort study

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0251159
Author(s):  
Ashraf Hassan Alzaabi ◽  
Luai A. Ahmed ◽  
Abdulla E. Rabooy ◽  
Ali Al Zaabi ◽  
Mohammed Alkaabi ◽  
...  
Keyword(s):  
Immune Response ◽  
Peak Level ◽  
Tertiary Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Antibody Titers ◽  
Consistent Increase ◽  
Specific Igg ◽  
Serum Igg Levels ◽  
Positive Immune Response

Objectives To quantify SARS-CoV2 IgG antibody titers over time and assess the longevity of the immune response in a multi-ethnic population setting. Setting This prospective study was conducted in a tertiary hospital in Abu Dhabi city, UAE, among COVID-19 confirmed patients. The virus-specific IgG were measured quantitatively in serum samples from the patients during three visits over a period of 6 months. Serum IgG levels ≥15 AU/ml was used to define a positive response. Participants 113 patients were analyzed at first visit, with a mean (SD) age of participants of 45.9 (11.8) years 87.5% of the patients were men. 63 and 27 participants had data available for visits 2 and 3, respectively. Primary outcome Change in SARS-CoV2 IgG antibody titers over the visits. Results No mortality or re-infection were reported. 69% of the patients developed positive IgG response within the first month after the onset of symptoms. The levels of IgG showed a consistent increase during the first three months with a peak level during the third month. Increasing trend in the levels of IgG were observed in 82.5%, 55.6% and 70.4% of patients between visit 1 to visit 2, visit 2 to visit 3, and from visit 1 to visit 3, respectively. Furthermore, about 64.3% of the patients showed sustained increase in IgG response for more than 120 days. Conclusions Our study indicates a sustained and prolonged positive immune response in COVID-19 recovered patients. The consistent rise in antibody and positive levels of IgG titers within the first 5 months suggest that immunization is possible, and the chances of reinfection minimal.

The Inducing Roles of the New Isolated Bursal Hexapeptide and Pentapeptide on the Immune Response of AIV Vaccine in Mice

2019 ◽  
Vol 26 (7) ◽  
pp. 542-549 ◽  
Author(s):  
Shan Shan Hao ◽  
Man Man Zong ◽  
Ze Zhang ◽  
Jia Xi Cai ◽  
Yang Zheng ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cells ◽  
Amino Acid ◽  
T Cell ◽  
Antigen Presentation ◽  
Amino Acid Sequences ◽  
Cell Subpopulation ◽  
Igg Antibody ◽  
Antibody Titers ◽  
Specific Igg

Background: Bursa of Fabricius is the acknowledged central humoral immune organ. The bursal-derived peptides play the important roles on the immature B cell development and antibody production. Objective: Here we explored the functions of the new isolated bursal hexapeptide and pentapeptide on the humoral, cellular immune response and antigen presentation to Avian Influenza Virus (AIV) vaccine in mice immunization. Methods: The bursa extract samples were purified following RP HPLC method, and were analyzed with MS/MS to identify the amino acid sequences. Mice were twice subcutaneously injected with AIV inactivated vaccine plus with two new isolated bursal peptides at three dosages, respectively. On two weeks after the second immunization, sera samples were collected from the immunized mice to measure AIV-specific IgG antibody levels and HI antibody titers. Also, on 7th day after the second immunization, lymphocytes were isolated from the immunized mice to detect T cell subtype and lymphocyte viabilities, and the expressions of co-stimulatory molecule on dendritic cells in the immunized mice. Results: Two new bursal hexapeptide and pentapeptide with amino acid sequences KGNRVY and MPPTH were isolated, respectively. Our investigation proved the strong regulatory roles of bursal hexapeptide on AIV-specific IgG levels and HI antibody titers, and lymphocyte viabilities, and the significant increased T cells subpopulation and expressions of MHCII molecule on dendritic cells in the immunized mice. Moreover, our findings verified the significantly enhanced AIV-specific IgG antibody and HI titers, and the strong increased T cell subpopulation and expressions of CD40 molecule on dendritic cells in the mice immunized with AIV vaccine and bursal pentapeptide. Conclusion: We isolated and identified two new hexapeptide and pentapeptide from bursa, and proved that these two bursal peptides effectively induced the AIV-specific antibody, T cell and antigen presentation immune responses, which provided an experimental basis for the further clinical application of the bursal derived active peptide on the vaccine improvement.

Measles Immunity After Revaccination: Results in Children Vaccinated Before 10 Months of Age

PEDIATRICS ◽  
1982 ◽  
Vol 69 (3) ◽  
pp. 332-335
Author(s):  
Calvin C. Linnemann ◽  
Mark S. Dine ◽  
Gary A. Roselle ◽  
P. Anne Askey
Keyword(s):  
Measles Virus ◽  
Geometric Mean ◽  
Lymphocyte Transformation ◽  
First Year ◽  
Cell Mediated Immunity ◽  
Measles Vaccine ◽  
Pediatric Practice ◽  
Antibody Titers ◽  
Measles Vaccination ◽  
First Year Of Life

Measles immunity was studied in children in a private pediatric practice who had been revaccinated because they had received their primary measles vaccination before 1 year of age. Antibody was measured in 72 of these children who had received the first injection of live measles virus vaccine at &lt;10 months of age, and the second at &gt;1 year of age. Of the 72 children, 29 (40%) had no detectable antibody and the geometric mean titer for the group was approximately 1:4. Of the children with low antibody titers, 15 were given a third injection of measles vaccine and five (33%) still did not respond. Cell- mediated immunity as indicated by lymphocyte transformation to measles antigen was measured in 11 of the children. Five (45%) had responses to measles antigen, but the responses did not correlate with the presence or absence of antibody. This study confirms the observation that revaccination is unsuccessful in many children who received measles vaccine in the first year of life, and shows that even a third injection of vaccine may fail to produce a significant antibody response.

scholarly journals Pattern of resilient age-related measles immunity

2020 ◽  
Vol 10 (2) ◽  
pp. 375-380
Author(s):  
A. G. Sonis ◽  
O. A. Gusyakova ◽  
F. N. Gilmiyarova ◽  
A. A. Ereshchenko ◽  
N. K. Ignatova ◽  
...  
Keyword(s):  
Measles Virus ◽  
Age Groups ◽  
Igg Antibody ◽  
Infection Level ◽  
Age Related ◽  
Specific Igg ◽  
Young Subjects ◽  
High Level ◽  
Medical University ◽  
Igg Level

Epidemiological situation describing global measles spread is ambiguous. Along with countries succeeded in measles eradication, there are those wherein measles rate remains at quite high level. Because measles is a vaccine-preventable infection, it may then be eradicated solely by ensuring sufficient population coverage with preventive vaccination. The aim of our study was to assess level of measles immunity in medical workers at the Clinics of Samara State Medical University as well as the Samara State Medical University. There were enrolled 1503 subjects (aged 18–79 years), among which all individuals under 55 (77.58%) but not older counterparts provided with medical record on previous measles vaccination or measles infection. Level of serum measles virus-specific IgG antibodies was measured by using ELISA (VektoKor-IgG, JSC Vector-Best, Novosibirsk), with mean concentration ranging in general population within 1.02±0.02 IU/ ml. Positive results were observed in 72.52% of the examined individuals. Average vs. high measles virus-specific IgG level was detected in 52.90% (mean age — 41.4±0.5 years) and 19.62% (mean age — 54.2±0.72 years) of individuals, whereas at level below threshold — in 27.48% of subjects (mean age — 33.25±0.53 years). Thus, in 34.16% of the surveyed vaccinated individuals mostly presented by young subjects contained anti-measles virus-specific antibodies below protective level. Older age groups were shown to increase in average IgG amount with age. Interestingly, age-related measles immunity pattern was observed: percentage of subjects with high vs. low measles virus-specific IgG level increases and decreases, respectively. Taking into consideration a large percentage of subjects previously vaccinated against measles among carriers of low measles immunity, it may be concluded that measles virus-specific IgG antibody level must be monitored in young adulthood to decide of whether subsequent revaccination is necessary.

scholarly journals Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan

2020 ◽  
Author(s):  
RIn Yokoyama ◽  
Makoto Kurano ◽  
Yoshifumi Morita ◽  
Takuya Shimura ◽  
Yuki Nakano ◽  
...  
Keyword(s):  
Measurement System ◽  
False Positive ◽  
Laboratory Testing ◽  
Antibody Test ◽  
Measurement Range ◽  
Antibody Assay ◽  
Serum Samples ◽  
Igg Antibody ◽  
Antibody Titers ◽  
Pcr Test

PCR methods are presently the standard for the diagnosis of Coronavirus disease 2019 (COVID-19), but additional methodologies are needed to complement PCR methods, which have some limitations. Here, we validated and investigated the usefulness of measuring serum antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the iFlash3000 CLIA analyzer. We measured IgM and IgG titers against SARS-CoV-2 in sera collected from 26 PCR-positive COVID-19 patients, 53 COVID-19-suspected but PCR-negative patients, and 20 and 100 randomly selected non-COVID-19 patients who visited our hospital in 2020 and 2017, respectively. The within-day and between-day precisions were regarded as good, since the coefficient variations were below 5%. Linearity was also considered good between 0.6 AU/mL and 112.7 AU/mL for SARS-CoV-2 IgM and between 3.2 AU/mL and 55.3 AU/mL for SARS-CoV-2 IgG, while the linearity curves plateaued above the upper measurement range. We also confirmed that the seroconversion and no-antibody titers were over the cutoff values in all 100 serum samples collected in 2017. These results indicate that this measurement system successfully detects SARS-CoV-2 IgM/IgG. We observed four false-positive cases in the IgM assay and no false-positive cases in the IgG assay when 111 serum samples known to contain autoantibodies were evaluated. The concordance rates of the antibody test with the PCR test were 98.1% for SARS-CoV-2 IgM and 100% for IgG among PCR-negative cases and 30.8% for SARS-CoV-2 IgM and 73.1% for SARS-CoV-2 IgG among PCR-positive cases. In conclusion, the performance of this measurement system is sufficient for use in laboratory testing.

Sign in / Sign up

Export Citation Format

Share Document