scholarly journals Polyhyrdoxybutyrate production by Bacillus marcorestinctum using a Cheaper substrate and its electrospinned blends with polymer

2019 ◽  
Author(s):  
Swetha Narayankumar ◽  
Neethu K. Shaji ◽  
Veena gayathri Krishnaswamy
Keyword(s):  
Carbon Source ◽  
Large Scale ◽  
C:N Ratio ◽  
Scale Up ◽  
Production Costs ◽  
Scale Production ◽  
Transmission Electron ◽  
Large Scale Production ◽  
Dry Cell Weight ◽  
Alternative Carbon Source

ABSTRACTPoly(hydroxybutyric acid) (PHB) and other biodegradable polyesters are promising candidates for the development of environment-friendly and completely biodegradable plastics. One of the major drawbacks in the production of PHB is production costs, since it requires large amount of carbon source. This calls for cheaper substrates that can be used as an alternative carbon source such as agro-industrial residues. In this study, cane molasses was used as an additional carbon source at 2% concentration along with glucose for large scale production of PHB. Ammonium nitrate was used as the nitrogen source and the C:N ratio was maintained at 1:15. The maximum production of PHB was obtained at 24hours of growth which was found to be 0.5g/L and had a dry cell weight of 3.7g/ L.The PHB produced was further analysed by GC-MS Analysis and Transmission Electron Microscopy (TEM).The obtained PHB from scale-up studies were further electrospinned using different blends of polymers.

scholarly journals Elevated β-Carotene Production Using Codon-Adapted CarRA&B and Metabolic Balance in Engineered Yarrowia lipolytica

2021 ◽  
Vol 12 ◽  
Author(s):  
Liang Liu ◽  
Yu Ling Qu ◽  
Gui Ru Dong ◽  
Jing Wang ◽  
Ching Yuan Hu ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Large Scale ◽  
Blakeslea Trispora ◽  
Scale Production ◽  
Total Production ◽  
Metabolic Balance ◽  
Carotene Content ◽  
Large Scale Production ◽  
Dry Cell Weight ◽  
Β Carotene

β-carotene is a precursor of vitamin A and has multiple physiological functions. Producing β-carotene by microbial fermentation has attracted much attention to consumers’ preference for natural products. This study focused on improving β-carotene production by constructing codon-adapted genes and minimizing intermediate accumulation. The codon-adapted CarRA and CarB genes from the industrial strain of Blakeslea trispora were integrated into the genome of the Yarrowia lipolytica to construct YL-C0, the baseline strain for producing β-carotene. Thereafter, the β-carotene biosynthetic pathway’s metabolic balance was accurately regulated to reduce the intermediates’ accumulation. Notably, the β-carotene content increased by 21 times to reach 12.5 dry cell weight (DCW) mg/g when minimizing HMG-CoA and FPP accumulation. Further, we improved the expression levels of the CarRA and CarB genes to minimize the accumulation of phytoene and lycopene. Total production of β-carotene of 1.7 g/L and 21.6 mg/g DCW was achieved. These results reveal that the rate-limiting enzymes CarRA and CarB of B. trispora exhibited higher catalytic activity than the same enzymes from other microorganisms. Promoting metabolic balance by minimizing the accumulation of intermediates is a very effective strategy for increasing β-carotene. The β-carotene-producing strain constructed in this study has established the foundation for its potential use in industrial production. These successful engineering strategies also provide a foundation for large-scale production of other terpenoids.

Phosphors for Various Dosimetry Applications Derived by Different Synthesis Routes

2018 ◽  
pp. 53-84
Author(s):  
Jyoti Singh ◽  
Mahesh S. Bhadane ◽  
Vikas Dubey ◽  
Sanjay Daga Dhole ◽  
Jairam Manam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Large Scale ◽  
Scale Production ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Large Scale Production ◽  
Wide Range ◽  
Co Precipitation ◽  
Dose Responses ◽  
Microscopy Techniques

The chapter provides useful information about synthesis and characterization of dysprosium doped oxide and fluoride-based phosphors such as SrGd2O4, CaSO4, and CaF2. Various techniques (e.g., acid-recrystallization, chemical co-precipitation, and homogenous precipitation cum auto-combustion methods) were adopted to synthesize these phosphors for large-scale production. All the prepared phosphors were characterized by x-ray diffraction analysis, scanning electron microscopy, and transmission electron microscopy techniques. The thermoluminescence (TL) studies were performed after different irradiation sources such as gamma rays, thermal neutrons, and low energy ions (H, Ar, and N), respectively. Linear dose responses were observed in a wide range of doses for all the samples. Various trapping parameters, namely order of kinetics, activation energy, and frequency factors, were calculated by using computerized glow curve deconvolution (CGCD) method.

scholarly journals Characterization of graphene nanosheets obtained by a modified Hummer's method

2018 ◽  
Vol 23 (1) ◽  
Author(s):  
Renata Hack ◽  
Cláudia Hack Gumz Correia ◽  
Ricardo Antônio de Simone Zanon ◽  
Sérgio Henrique Pezzin
Keyword(s):  
Electron Microscopy ◽  
Large Scale ◽  
Graphene Nanosheets ◽  
Scale Production ◽  
Natural Graphite ◽  
Graphite Oxidation ◽  
Transmission Electron ◽  
Large Scale Production ◽  
High Degree

ABSTRACT Natural graphite is an inexpensive and abundant source to obtain graphene nanosheets. The most efficient method for large-scale production is the chemical method, which is based on the oxidation of natural graphite. This paper reports the synthesis and characterization of graphene obtained by the Hummers method with some modifications. The results indicate a high degree of graphite oxidation, proving that the process was efficient. Analyses of field emission scanning electron microscopy (FEG), transmission electron microscopy (TEM), Raman spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction showed that the graphene produced presented characteristics similar to the commercial graphene.

Synthesis and characterization of Y2O3: Eu3+ powder phosphor by a hydrolysis technique

1998 ◽  
Vol 13 (10) ◽  
pp. 2950-2955 ◽  
Author(s):  
Yong Dong Jiang ◽  
Zhong Lin Wang ◽  
Fuli Zhang ◽  
Henry G. Paris ◽  
Christopher J. Summers
Keyword(s):  
Electron Microscopy ◽  
Large Scale ◽  
Luminescent Properties ◽  
Industrial Applications ◽  
Europium Oxide ◽  
Scale Production ◽  
Experimental Conditions ◽  
Luminescence Efficiency ◽  
Transmission Electron ◽  
Large Scale Production

A forced hydrolysis technique is used for preparing Y2O3: Eu3+ powders at low processing temperatures. The technique uses yttrium oxide, europium oxide, and nitric acid and urea, and has the potential for large-scale production for industrial applications. Several experimental conditions have been examined to optimize the luminescence efficiency. The best result was found to be at 2 mol% Eu doping and a 2 h firing of 1400 °C. Microstructural information provided by x-ray diffraction, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) have been applied to interpret the observed luminescent properties.

scholarly journals Rapid synthesis and characterization of silver-loaded graphene oxide nanomaterials and their antibacterial applications

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Jiang Zhu ◽  
Haitao Ni ◽  
Chunyan Hu ◽  
Yuxiang Zhu ◽  
Jinxia Cai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Graphene Oxide ◽  
Large Scale ◽  
Structural Characteristics ◽  
Water Soluble ◽  
Scale Production ◽  
Energy Dispersion Spectroscopy ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Large Scale Production

With the promising potential application of Ag/graphene-based nanomaterials in medicine and engineering materials, the large-scale production has attracted great interest of researchers on the basis of green synthesis. In this study, water-soluble silver/graphene oxide (Ag/GO) nanomaterials were synthesized under ultrasound-assisted conditions. The structural characteristics of Ag/GO were confirmed by Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy and energy dispersion spectroscopy, respectively. The results showed the silver particles (AgNPs) obtained by reduction were attached to the surface of GO, and there was a strong interaction between AgNPs and GO. The antibacterial activity was primarily evaluated by the plate method and hole punching method. Antibacterial tests indicated that Ag/GO could inhibit the growth of Gram-negative and Gram-positive bacteria, special for the Staphylococcus aureus .

Production ofBacillus amyloliquefaciensOG and its metabolites in renewable media: valorisation for biodiesel production andp-xylene decontamination

2017 ◽  
Vol 63 (1) ◽  
pp. 46-60 ◽  
Author(s):  
Augusto Etchegaray ◽  
François Coutte ◽  
Gabrielle Chataigné ◽  
Max Béchet ◽  
Ramon H.Z. dos Santos ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Large Scale ◽  
Production Costs ◽  
Biodiesel Production ◽  
Scale Production ◽  
Specific Production ◽  
Primary And Secondary Metabolites ◽  
Large Scale Production ◽  
Promote Plant Growth ◽  
Acetoin Production

Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after analysis of the gyrA and gyrB DNA sequences. Growth in modified Landy’s medium produced 3 main recoverable metabolites: surfactin, fengycin, and acetoin, which promote plant growth. Cultivation was studied in the presence of renewable carbon (as glycerol) and nitrogen (as arginine) sources. While diverse kinetics of acetoin production were observed in different media, similar yields (6–8 g·L–1) were obtained after 72 h of growth. Glycerol increased surfactin-specific production, while arginine increased the yields of surfactin and fengycin and increased biomass significantly. The specific production of fengycin increased ∼10 times, possibly due to a connecting pathway involving arginine and ornithine. Adding value to crude extracts and biomass, both were shown to be useful, respectively, for the removal of p-xylene from contaminated water and for biodiesel production, yielding ∼70 mg·g–1cells and glycerol, which could be recycled in novel media. This is the first study considering circular bioeconomy to lower the production costs of biosurfactants by valorisation of both microbial cells and their primary and secondary metabolites.

scholarly journals Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
A. Barros ◽  
H. Pereira ◽  
J. Campos ◽  
A. Marques ◽  
J. Varela ◽  
...  
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Biomass Concentration ◽  
Growth Conditions ◽  
Pilot Scale ◽  
Industrial Scale ◽  
Scale Production ◽  
Two Stage ◽  
Chlorophyll Contents ◽  
Large Scale Production

Abstract Industrial scale-up of microalgal cultures is often a protracted step prone to culture collapse and the occurrence of unwanted contaminants. To solve this problem, a two-stage scale-up process was developed – heterotrophically Chlorella vulgaris cells grown in fermenters (1st stage) were used to directly inoculate an outdoor industrial autotrophic microalgal production unit (2nd stage). A preliminary pilot-scale trial revealed that C. vulgaris cells grown heterotrophically adapted readily to outdoor autotrophic growth conditions (1-m3 photobioreactors) without any measurable difference as compared to conventional autotrophic inocula. Biomass concentration of 174.5 g L−1, the highest value ever reported for this microalga, was achieved in a 5-L fermenter during scale-up using the heterotrophic route. Inocula grown in 0.2- and 5-m3 industrial fermenters with mean productivity of 27.54 ± 5.07 and 31.86 ± 2.87 g L−1 d−1, respectively, were later used to seed several outdoor 100-m3 tubular photobioreactors. Overall, all photobioreactor cultures seeded from the heterotrophic route reached standard protein and chlorophyll contents of 52.18 ± 1.30% of DW and 23.98 ± 1.57 mg g−1 DW, respectively. In addition to providing reproducible, high-quality inocula, this two-stage approach led to a 5-fold and 12-fold decrease in scale-up time and occupancy area used for industrial scale-up, respectively.

scholarly journals A Novel Approach Using Conventional Methodologies to Scale up BNC Production Using Komagataeibacter medellinensis and Rotten Banana Waste as Alternative

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1469
Author(s):  
Carlos Molina-Ramírez ◽  
Juan Álvarez ◽  
Robin Zuluaga ◽  
Cristina Castro ◽  
Piedad Gañán
Keyword(s):  
Production Process ◽  
Large Scale ◽  
Culture Medium ◽  
Scale Up ◽  
Glucose Consumption ◽  
Human Consumption ◽  
Scale Production ◽  
Bacterial Nanocellulose ◽  
Large Scale Production ◽  
Glucose Diffusion

Currently, cellulose nanostructures are among the most promising structures, and extensive work in materials and biotechnology industries is aimed at identifying an efficient process of production. Even when production at the laboratory scale is successful, crucial aspects of increased commercial applications for cellulose nanostructures are linked to large-scale production. Large-scale production requires a balance between the cost of the culture medium and product value. Therefore, in this work, for the optimization and scaling up of bacterial nanocellulose, a culture medium consisting of rotten banana unsuitable for human consumption was used for the first time as an inexpensive feedstock. Initially, the bacterial nanocellulose (BNC) culture medium conditions were optimized, and it was established that a glucose concentration of 26.4 g/L and a V/A ratio of 2.2 cm were the optimal conditions for production reaching a BNC yield of 5 g/L, which was 42.4% higher than the best result initially obtained. Finally, the scale-up process was performed, implementing a regime analysis methodology by comparing the characteristic times of the critical mechanisms involved in BNC production, namely, microbial growth, glucose consumption, BNC production, and glucose diffusion into the BNC membrane, as the first approach for this type of BNC production process. The mechanism underlying the BNC production process is glucose diffusion into the BNC membrane (characteristic time, 675.47 h). Thus, the V/A ratio was selected as the scale-up criterion most suitable for producing BNC under static culture conditions, allowing the production of 16 g of BNC after 12 d of fermentation in a plastic bioreactor, which was 3378% higher than that produced in glass vessels. The results obtained in this study may initiate further improvements in BNC commercial production by exploiting different feedstocks.

scholarly journals Scale-up of Electrospinning: Market Overview of Products and Devices for Pharmaceutical and Biomedical Purposes

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 286
Author(s):  
Safaa Omer ◽  
László Forgách ◽  
Romána Zelkó ◽  
István Sebe
Keyword(s):  
Pharmaceutical Industry ◽  
Production Rate ◽  
Drug Delivery Systems ◽  
Large Scale ◽  
Hybrid Methods ◽  
Scale Up ◽  
Scale Production ◽  
Additional Energy ◽  
Large Scale Production ◽  
Potential Applications

Recently, the electrospinning (ES) process has been extensively studied due to its potential applications in various fields, particularly pharmaceutical and biomedical purposes. The production rate using typical ES technology is usually around 0.01–1 g/h, which is lower than pharmaceutical industry production requirements. Therefore, different companies have worked to develop electrospinning equipment, technological solutions, and electrospun materials into large-scale production. Different approaches have been explored to scale-up the production mainly by increasing the nanofiber jet through multiple needles, free-surface technologies, and hybrid methods that use an additional energy source. Among them, needleless and centrifugal methods have gained the most attention and applications. Besides, the production rate reached (450 g/h in some cases) makes these methods feasible in the pharmaceutical industry. The present study overviews and compares the most recent ES approaches successfully developed for nanofibers’ large-scale production and accompanying challenges with some examples of applied approaches in drug delivery systems. Besides, various types of commercial products and devices released to the markets have been mentioned.

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