scholarly journals Effects of anesthetic tricaine on stress and reproductive aspects of South American silver catfish (Rhamdia quelen) male

2019 ◽  
Author(s):  
Nathalia dos Santos Teixeira ◽  
Lis Santos Marques ◽  
Rômulo Batista Rodrigues ◽  
Darlan Gusso ◽  
Ana Amélia Nunes Fossati ◽  
...  
Keyword(s):  
Induction Time ◽  
Plasma Cortisol ◽  
Sperm Quality ◽  
Reproductive Hormones ◽  
Anesthesia Induction ◽  
South American ◽  
Silver Catfish ◽  
Rhamdia Quelen ◽  
Cortisol Levels ◽  
Motility Rate

AbstractAnesthesia is a common practice used in fish research and aquaculture. For both applications, it is important to understand anesthetics effects on the animal and tissues of interest to ensure the validity of data and to improve animal welfare. Captive fish production is only possible with artificial reproduction, and it is known that manipulation is a stressor stimulus in fish. The most common method of determining fish stress responses is measuring the circulating level of cortisol. Therefore, the purpose of this study was to evaluate the effects of different concentrations (100, 200, and 300 mg L-1) of the anesthetic tricaine methanesulfonate (MS-222) on cortisol levels and their influence on the sperm quality maintenance in Rhamdia quelen. After hormonal induction, 28 sexually mature males (average weight = 363.00 ± 71.24 g) were randomly distributed among treatments, and their semen and blood samples were collected. Anesthesia induction time, motility rate, sperm concentration and morphology, plasma cortisol levels, and reproductive hormones concentrations (testosterone, 17-α-hydroxyprogesterone, and estradiol) were evaluated. Anesthesia with 100 mg L-1 MS-222 presented a longer induction time than that with 200 and 300 mg L-1 MS-222. Sperm motility rate was significantly higher in the control than in the 300 mg L-1 treatment but did not differ among the control, 100, and 200 mg L-1 treatments. Estradiol level was significantly higher in non-anesthetized than in anesthetized fish, but plasma cortisol levels did not differ significantly between treatments (182.50 ± 42.03 ng mL-1). MS-222 anesthetizes fish by blocking the sodium channels, preventing the development of nerve action potentials. However, MS222 at concentrations of 100, 200, and 300 mg L-1 did not prevent stress in South American silver catfish males. In addition, its use did not maintain sperm quality, as it impaired motility and decreased levels of plasma estradiol.

Effects of different doses of eugenol on plasma cortisol levels and the quality of fresh and frozen-thawed sperm in South American catfish (Rhamdia quelen)

2019 ◽  
Vol 125 ◽  
pp. 135-139 ◽  
Author(s):  
Maira N. Corso ◽  
Lis S. Marques ◽  
Luis F.G. Gracia ◽  
Rômulo B. Rodrigues ◽  
Leonardo J.G. Barcellos ◽  
...  
Keyword(s):  
Plasma Cortisol ◽  
South American ◽  
Rhamdia Quelen ◽  
Cortisol Levels ◽  
Different Doses

scholarly journals Activity patterns of South American silver catfish (Rhamdia quelen)

2006 ◽  
Vol 66 (2a) ◽  
pp. 565-574 ◽  
Author(s):  
U. H. Schulz ◽  
C. Leuchtenberger
Keyword(s):  
Woody Debris ◽  
Activity Patterns ◽  
Swimming Activity ◽  
Fish Length ◽  
South American ◽  
Silver Catfish ◽  
Frequent Use ◽  
Rhamdia Quelen ◽  
Lakes And Reservoirs ◽  
The Mean

The South American silver catfish (Rhamdia quelen) is a widely distributed species in Central and South America in areas east of the Andes between Venezuela and the northern parts of Argentina. The bottom dwelling species occurs in lakes and reservoirs as well as in rivers. Between June 2000 and December 2001, sixteen silver catfish were tracked during fourteen 24-h cycles in two-hour-intervals, with the aim of investigating daily movements and habitat use. Covered distances varied between 0 m/2 h and 326 m/2 h and the mean distance covered in 2 h was 25.6 m. The mean activity of individual silver catfish varied between 5.6 m/2 h and 81.4 m/2 h. The swimming activity was linearly related to the total fish length. The highest mean swimming activity occurred in the morning and at nightfall. Silver catfish concentrated in three areas of frequent use. All of them were characterized by steep banks providing shelter in the form of rip-rap or large woody debris. Vertically, silver catfish preferred the upper 2 m layer where tracked fish encountered higher temperatures and higher dissolved oxygen concentrations.

Cryopreservation‐induced morphological changes in the sperm of South American silver catfish ( Rhamdia quelen )

2019 ◽  
Author(s):  
Bruna B. da Costa ◽  
Lis S. Marques ◽  
Paula G. Lassen ◽  
Rômulo B. Rodrigues ◽  
Danilo P. Streit
Keyword(s):  
Morphological Changes ◽  
South American ◽  
Silver Catfish ◽  
Rhamdia Quelen

scholarly journals Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
Carine de Freitas Souza ◽  
Matheus Dellaméa Baldissera ◽  
Joseânia Salbego ◽  
Jane Mello Lopes ◽  
Rodrigo de Almeida Vaucher ◽  
...  
Keyword(s):  
Blood Gases ◽  
Control Fish ◽  
Control Group ◽  
Anesthesia Induction ◽  
Electrolyte Balance ◽  
Plasma Parameters ◽  
Silver Catfish ◽  
Lippia Alba ◽  
Rhamdia Quelen ◽  
Recovery Times

ABSTRACT The aim of this study was to evaluate if Lippia alba has different chemotypes according to the chemical composition of the essential oil (EO) considering collection site, and if the EO may have different effects on blood and plasma parameters in silver catfish, Rhamdia quelen, during and immediately after anesthesia. The citral (EO-C) and linalool (EO-L) chemotypes were identified, and both presented similar anesthetic effects for silver catfish. Fish were exposed to two concentrations of each EO, which induced slow and fast anesthesia (100 and 300 µL L-1, respectively). Blood ions did not change at any time of anesthesia induction and recovery and, therefore, the electrolyte balance was not altered. Blood gases oscillated through all exposure and recovery times, but there was an increase in pO2 after 10 min recovery in fish anesthetized with EO-C. Glucose increased in fish exposed to both EOs when compared with the control group. Overall, exposure to both EOs (except 100 µL L-1 EO-L at most times) reduced plasma cortisol levels compared to the control and/or ethanol groups. However, as plasma creatinine levels in fish anesthetized with EO-C were higher than control fish, the use of EO-L is preferable.

Effects of anesthetic MS-222 on stress and reproduction of South American silver catfish (Rhamdia quelen) males

2021 ◽  
Vol 225 ◽  
pp. 106669
Author(s):  
Nathalia dos Santos Teixeira ◽  
Lis Santos Marques ◽  
Rômulo Batista Rodrigues ◽  
Darlan Gusso ◽  
Gabriela Thais Pinheiro ◽  
...  
Keyword(s):  
South American ◽  
Silver Catfish ◽  
Rhamdia Quelen

scholarly journals Essential oils from Citrus x aurantium and Citrus x latifolia (Rutaceae) have anesthetic activity and are effective in reducing ion loss in silver catfish ( Rhamdia quelen )

2018 ◽  
Vol 16 (2) ◽  
Author(s):  
Jane Mello Lopes ◽  
Carine de Freitas Souza ◽  
Bianca Schindler ◽  
Carlos Garrido Pinheiro ◽  
Joseânia Salbego ◽  
...  
Keyword(s):  
Essential Oils ◽  
Ammonia Excretion ◽  
Fish Mortality ◽  
Control Fish ◽  
Control Group ◽  
Anesthesia Induction ◽  
Anesthetic Activity ◽  
Silver Catfish ◽  
Anesthetic Effect ◽  
Rhamdia Quelen

ABSTRACT This study investigated the anesthetic effect of the essential oils (EOs) from the peel of Citrus x aurantium (EOCA) and Citrus x latifolia (EOCL) on silver catfish Rhamdia quelen. Fish were exposed to different concentrations of EOCA and EOCL to determine time of anesthesia induction and recovery. Induction of anesthesia was observed in all fish exposed to 400, 600 or 800 μL L−1 EOCA and 300, 400 or 500 μL L−1 EOCL. Another group of fish were exposed for 8 h to 50, 100, or 200 μL L−1 of either EOs. Overall, fish exposed to ethanol and both EOs presented higher ventilatory frequencies (VF) than the control group throughout the 8 h of exposure. Net ion (Na+, K+ and Cl−) effluxes and ammonia excretion were significantly lower in fish exposed to 50, 100 or 200 μL L−1 of either EOs compared to control fish. Mortality was 37% in fish exposed to 200 μL L−1 of either EOs after 8 h. These findings suggest that EOCA and EOCL are useful anesthetics and sedatives for Rhamdia quelen, but their usefulness as alternatives to reduce stress in fish transportation at the lower concentrations tested (50-100 µL L−1) deserves further study.

scholarly journals Extenders with vitamins C and E applied to Rhamdia quelen sperm cryopreservation / Extensores com vitaminas C e E aplicados à criopreservação de esperma de Rhamdia quelen

2021 ◽  
Vol 7 (12) ◽  
pp. 118046-118060
Author(s):  
Alan Müller Mendonça Xavier ◽  
Giovano Neumann ◽  
Eduardo Antônio Sanches ◽  
Sara Ugulino Cardoso ◽  
Robie Allan Bombardelli
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Sperm Quality ◽  
Milk Powder ◽  
Sperm Cryopreservation ◽  
Straight Line ◽  
Rhamdia Quelen ◽  
Different Levels ◽  
Velocity Average ◽  
Motility Rate

We performed this experiment to evaluate the effects of adding vitamins C and E on extenders for sperm cryopreservation of Rhamdia quelen over spermatic mobility after thawing. At cryopreservation, sperm samples were diluted in a proportion of 1:3 (v/v), following pre-freezing in nitrogen steam and subsequent immersion in liquid nitrogen. The diluents were composed by 5% milk powder, 5% glucose, 10% methanol and different levels of vitamin. Three sperm cryopreservation tests were carried out with (1) diluent containing 0.0; 4.0; 6.5; 9.0 and 11.5 mg of vitamin C mL-1, (2) diluent containing 0.0; 2.0; 4.0; 6.0 and 8.0 mg of vitamin E mL-1; (3) diluent containing 0.0; 4.0 + 2.0; 6.5 + 4.0; 9.0 + 6.0 and 11.5 + 8.0 mg of vitamin C mL-1 plus vitamin E mL-1, respectively. The spermatic motility rate, spermatic curvilinear velocity, average path and straight line velocities were measured in thawed semen by CASA. Data were submitted to ANOVA and Duncan´s test at 5% of significance. After thawing the effect (P0.05) of vitamin C was observed only for sperm motility, with higher values (38.2±20.7%) on solution containing 4.0 mg of vitamin C mL-1. The concomitant addition of both vitamins influenced (P0.05) only the curvilinear velocity, reducing the velocity at any concentration. In conclusion, diluents with 4.0 mg vitamin C mL-1 to cryopreservation of the silver catfish semen improve the sperm quality after thawing, and the use of diluents with vitamin E or both vitamins are not recommended because do not ensure the cells protection.

scholarly journals Acute toxicity test of agricultural pesticides on silver catfish (Rhamdia quelen) fingerlings

2008 ◽  
Vol 38 (4) ◽  
pp. 1050-1055 ◽  
Author(s):  
Luiz Carlos Kreutz ◽  
Leonardo José Gil Barcellos ◽  
Tális Oliveira Silva ◽  
Deniz Anziliero ◽  
Daiana Martins ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Environmental Protection Agency ◽  
Fish Mortality ◽  
South American ◽  
Immunological Parameters ◽  
Silver Catfish ◽  
Agricultural Pesticides ◽  
Toxic Concentration ◽  
Long Term Effect ◽  
Rhamdia Quelen

Toxicity risks of agricultural pesticides to fishes are pivotal. Currently, many questions remain unsolved regarding to the toxicity of commonly used pesticides to silver catfish (Rhamdia quelen), a South American catfish. The present studies have been designed to investigate the acute toxicity and the lethal concentration (LC50) of four herbicides, two fungicides and two insecticides to silver catfish fingerlings. All experiments were carried out in triplicates, in a static bioassay system, using commercially available pesticides. The data was analyzed through the Trimmed Spearman-Karber method available from the Environmental Protection Agency. The 96hLC50 and 95% lower and upper confidence limits, respectively, for the following pesticides were determined: glyphosate (7.3mg L-1; 6.5-8.3), atrazine (10.2mg L-1; 9.1-11.5), atrazine+simazine (10.5mg L-1; 8.9-12.4), mesotrione (532.0mg L-1; 476.5-594), tebuconazole (5.3mg L-1; 4.9-5.7), methylparathion (4.8mg L-1; 4.3-5.3), strobulurin and triazol (9.9mg L-1; 8.7-11.2). Diflubenzuron was also tested and caused no fish mortality up to 1g L-1. The toxic concentration of these pesticides to silver catfish fingerlings fell above the concentration used for application in the field and, except following accidental application or misplacing of empty recipients, it should not cause fish mortality. Nonetheless, the data obtained will be useful to study the long-term effect of these products on the hematological, biochemical, hormonal and immunological parameters of silver catfish and related fish species in South Brazil.

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