Low-Fat Beef Patties with Augmented Omega-3 Fatty Acid and CLA Levels and Influence of Grape Seed Extract

Abstract Background Familial apo C-II deficiency is a rare hereditary disorder frequently caused by lipoprotein lipase (LPL) and APOC2 gene mutations. To date, less than 30 patients with familial apo C-II deficiency with 24 different mutations have been identified in the literature. Here, we describe two familial chylomicronemia syndrome cases in infants with two novel mutations of the APOC2 gene. Case presentation Case 1, a 46-day-old female, was admitted to our hospital for evaluation due to the lipemic appearance of the blood sample. A clinical examination revealed hepatomegaly and lipemia retinalis. Triglyceride level of 6295 mg/dL was decreased with a strict low-fat diet, medium-chain triglycerides (MCT) oil-rich formula and omega-3 fatty acid supplementation. Due to low adherence to the diet, TG elevation was detected and fresh frozen plasma (10 mL/kg/day) was administered for 2 days. A novel homozygous p.Q25X (c.73C>T) mutation in the APOC2 gene was detected. Case 2, a 10-month-old female patient, referred to our center for the differential diagnosis of hyperlipidemia as her blood sample could not be assessed due to its lipemic appearance. Laboratory examinations showed a TG level of 4520 mg/dL which was reduced with a low-fat diet, MCT oil-rich formula and omega-3 fatty acid supplementation. Hepatosteatosis and splenomegaly were determined using abdominal sonography. A novel homozygous IVS2+6T>G (c.55+6T>G) mutation in the APOC2 gene was identified. Conclusions We describe two novel homozygous mutations (p.Q25X [c.73C>T] and IVS2+6T>G [c.55+6T>G]) in the APOC2 gene in infants with hyperchylomicronemia. To the best of our knowledge, Case 1 is the youngest patient with familial apo C-II deficiency in the literature to date.

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Effect of Grape Seed Extract, Cistus ladanifer L., and Vegetable Oil Supplementation on Fatty Acid Composition of Abomasal Digesta and Intramuscular Fat of Lambs

Journal of Agricultural and Food Chemistry ◽

10.1021/jf1021626 ◽

2010 ◽

Vol 58 (19) ◽

pp. 10710-10721 ◽

Cited By ~ 40

Author(s):

Eliana Jerónimo ◽

Susana P. Alves ◽

Maria T. P. Dentinho ◽

Susana V. Martins ◽

José A. M. Prates ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Vegetable Oil ◽

Grape Seed Extract ◽

Grape Seed ◽

Intramuscular Fat ◽

Seed Extract ◽

Cistus Ladanifer

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Effects of natural antioxidants on the stability of omega-3 fatty acids in dog food

Journal of Veterinary Research ◽

10.2478/jvetres-2018-0014 ◽

2018 ◽

Vol 62 (1) ◽

pp. 103-108 ◽

Cited By ~ 4

Author(s):

Florentina Glodde ◽

Mevlüt Günal ◽

Mary E. Kinsel ◽

Amer AbuGhazaleh

Keyword(s):

Thiobarbituric Acid ◽

Natural Antioxidants ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Omega 3 ◽

Dog Food ◽

Acai Berry ◽

Tbars Values ◽

The Stability

AbstractIntroductionThe efficiency of five natural antioxidants (curcumin, cranberry, pomegranate, grape seed extract (GSE), and açai berry) in reducing lipid oxidation in dog food was compared to that of the synthetic antioxidant butylated hydroxyanisole (BHA).Material and MethodsIn two different experiments content parameters were measured after 12 days of storage at 55°C. In experiment one, the natural antioxidants were added at 0.2% and BHA at 0.02% of the food (DM basis), and samples were analysed for thiobarbituric acid-reactive substances (TBARS). In experiment two, the effects of GSE and curcumin at two admixture proportions (0.1% and 0.2% of food DM) on omega-3 fatty acid (FA) content were evaluated.ResultsTBARS values were lower than the control (P < 0.01) for curcumin, cranberry, pomegranate, and GSE but not for the açai berry (P > 0.05). By day 12, although there were no significant differences (P > 0.05) between the two curcumin treatments, they preserved higher concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (P < 0.05) than the BHA and control treatments. The addition of GSE or BHA to dog food held (P < 0.05) the concentrations of EPA higher than the control. The concentrations of EPA and DHA for the 0.2% GSE treatment were greater (P < 0.05) than the 0.1% GSE treatment. Grape seed extract at 0.2% lost less (P < 0.05) EPA concentration than BHA.ConclusionThe present results showed that, except for açai berry, the tested natural antioxidants could be used as a substitute for BHA in dog food.

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Incorporation of omega‐3 fatty acid‐rich grape seed oil in yoghurt: Response surface optimization of physicochemical, textural, and sensory attributes during refrigerated storage

Food Science & Nutrition ◽

10.1002/fsn3.1998 ◽

2020 ◽

Author(s):

Atousa Kokabian ◽

Amir Daraei Garmakhany ◽

Shima Jafarzadeh ◽

Narjes Aghajani

Keyword(s):

Fatty Acid ◽

Response Surface ◽

Seed Oil ◽

Grape Seed ◽

Sensory Attributes ◽

Refrigerated Storage ◽

Omega 3 ◽

Omega 3 Fatty Acid ◽

Response Surface Optimization ◽

Grape Seed Oil

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Effects of Fish Oil and Grape Seed Extract Combination on Hepatic Endogenous Antioxidants and Bioactive Lipids in Diet-Induced Early Stages of Insulin Resistance in Rats

Marine Drugs ◽

10.3390/md18060318 ◽

2020 ◽

Vol 18 (6) ◽

pp. 318 ◽

Cited By ~ 1

Author(s):

Núria Taltavull ◽

Bernat Miralles-Pérez ◽

Maria Rosa Nogués ◽

Sara Ramos-Romero ◽

Lucía Méndez ◽

...

Keyword(s):

Insulin Resistance ◽

Fatty Acid ◽

Fish Oil ◽

Polyunsaturated Fatty Acid ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Standard Diet ◽

Early Stages ◽

Endogenous Antioxidants

Diacylglycerols (DAG) and ceramides have been suggested as early predictors of insulin resistance. This study was aimed to examine the combined effects of fish oil (FO) and grape seed extract (GSE) on hepatic endogenous antioxidants, DAG and ceramides in diet-induced early stages of insulin resistance. Thirty-five rats were fed one of the following diets: (1) a standard diet (STD group), (2) a high-fat high-sucrose diet (HFHS group), (3) an HFHS diet enriched with FO (FO group), (4) an HFHS diet enriched with GSE (GSE group) or (5) an HFHS diet enriched with FO and GSE (FO + GSE group). In the liver, endogenous antioxidants were measured using spectrophotometric and fluorometric techniques, and non-targeted lipidomics was conducted for the assessment of DAG and ceramides. After 24 weeks, the FO + GSE group showed increased glutathione peroxidase activity, as well as monounsaturated fatty acid and polyunsaturated fatty acid-containing DAG, and long-chain fatty acid-containing ceramides abundances compared to the STD group. The FO and GSE combination induced similar activation of the antioxidant system and bioactive lipid accumulation in the liver than the HFHS diet without supplementation. In addition, the FO and GSE combination increased the abundances of polyunsaturated fatty acid-containing DAG in the liver.

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