Effect of krill phospholipids versus soybean lecithin in microdiets for gilthead seabream (Sparus aurata) larvae on molecular markers of antioxidative metabolism and bone development

2014 ◽  
Vol 21 (4) ◽  
pp. 474-488 ◽  
Author(s):  
R. Saleh ◽  
M.B. Betancor ◽  
J. Roo ◽  
V. Benítez-Dorta ◽  
M.J. Zamorano ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sparus Aurata ◽  
Bone Development ◽  
Soybean Lecithin ◽  
Gilthead Seabream ◽  
Antioxidative Metabolism

Optimum soybean lecithin contents in microdiets for gilthead seabream (Sparus aurata) larvae

2013 ◽  
Vol 19 (4) ◽  
pp. 585-597 ◽  
Author(s):  
R. Saleh ◽  
M.B. Betancor ◽  
J. Roo ◽  
C.M. Hernandez-Cruz ◽  
F.-J. Moyano ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Soybean Lecithin ◽  
Gilthead Seabream

scholarly journals Effects of dietary DHA and α-tocopherol on bone development, early mineralisation and oxidative stress in Sparus aurata (Linnaeus, 1758) larvae

2012 ◽  
Vol 109 (10) ◽  
pp. 1796-1805 ◽  
Author(s):  
M. S. Izquierdo ◽  
M. Scolamacchia ◽  
M. Betancor ◽  
J. Roo ◽  
M. J. Caballero ◽  
...  
Keyword(s):  
Bone Formation ◽  
Sparus Aurata ◽  
Bone Development ◽  
Larval Growth ◽  
Gilthead Seabream ◽  
Skeletal Deformities ◽  
Skeletal Malformations ◽  
Expression Of Genes ◽  
Level Increase ◽  
Urinary Bladder Calculi

DHA deficiency has been related to skeletal malformations in fish, but high DHA levels have produced controversial results that could relate to the oxidative status of fish tissues in the different reports. In the present study, gilthead seabream (Sparus aurata) larvae were fed deficient, adequate or high DHA levels, or high DHA levels supplemented with the antioxidant α-tocopherol. Larvae fed deficient DHA levels tended to be smaller, and showed the highest incidence of urinary bladder calculi, lordosis and kyphosis and the lowest number of mineralised vertebrae for any given size class. Elevation of dietary DHA increased larval growth and significantly enhanced the expression of the insulin-like growth factor 1 (IGF-1) gene. However, a DHA level increase up to 5 % raised the degree of lipid oxidation in larval tissues and deformities in cranial endochondral bones and in axial skeletal haemal and neural arches. The increase in dietary α-tocopherol supplementation in high-DHA feeds reduced again the occurrence of skeletal deformities. Moreover, the expression of genes coding for specific antioxidants such as catalase, superoxide dismutase or glutathione peroxidase, which neutralised reactive oxygen substances formed by increased dietary DHA, was significantly decreased in larvae fed high α-tocopherol levels. These results denoted the importance of DHA for early bone formation and mineralisation. Low dietary DHA levels delay early mineralisation and increase the risk of cranial and axial skeletal deformities. Excessive DHA levels, without an adequate balance of antioxidant nutrients, increase the production of free radicals damaging cartilaginous structures before bone formation.

Effects of subcutaneous injection of λ/κ‐carrageenin on the immune and liver antioxidant status of gilthead seabream ( Sparus aurata )

2021 ◽  
Author(s):  
José Carlos Campos‐Sánchez ◽  
Francisco A. Guardiola ◽  
José María García Beltrán ◽  
Diana Ceballos‐Francisco ◽  
María Ángeles Esteban
Keyword(s):  
Subcutaneous Injection ◽  
Antioxidant Status ◽  
Sparus Aurata ◽  
Gilthead Seabream

scholarly journals Aflatoxicosis Dysregulates the Physiological Responses to Crowding Densities in the Marine Teleost Gilthead Seabream (Sparus aurata)

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 753
Author(s):  
Andre Barany ◽  
Juan Fuentes ◽  
Gonzalo Martínez-Rodríguez ◽  
Juan Miguel Mancera
Keyword(s):  
Mrna Expression ◽  
Sparus Aurata ◽  
Stocking Density ◽  
Head Kidney ◽  
Dietary Exposure ◽  
Experimental Period ◽  
Gilthead Seabream ◽  
Marine Teleost ◽  
Lipid Metabolites ◽  
Period Duration

Several studies in fish have shown that aflatoxin B1 (AFB1) causes a disparity of species-dependent physiological disorders without compromising survival. We studied the effect of dietary administration of AFB1 (2 mg AFB1 kg−1 diet) in gilthead seabream (Sparus aurata) juveniles in combination with a challenge by stocking density (4 vs. 40 g L−1). The experimental period duration was ten days, and the diet with AFB1 was administered to the fish for 85 days prior to the stocking density challenge. Our results indicated an alteration in the carbohydrate and lipid metabolites mobilization in the AFB1 fed group, which was intensified at high stocking density (HSD). The CT group at HSD increased plasma cortisol levels, as expected, whereas the AFB1-HSD group did not. The star mRNA expression, an enzyme involved in cortisol synthesis in the head kidney, presented a ninefold increase in the AFB1 group at low stocking density (LSD) compared to the CT-LSD group. Adenohypophyseal gh mRNA expression increased in the AFB1-HSD but not in the CT-HSD group. Overall, these results confirmed that chronic AFB1 dietary exposure alters the adequate endocrinological physiological cascade response in S. aurata, compromising the expected stress response to an additional stressor, such as overcrowding.

scholarly journals Influence of Genetic Selection for Growth and Broodstock Diet n-3 LC-PUFA Levels on Reproductive Performance of Gilthead Seabream, Sparus aurata

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 519
Author(s):  
Shajahan Ferosekhan ◽  
Serhat Turkmen ◽  
Cathaysa Pérez-García ◽  
Hanlin Xu ◽  
Ana Gómez ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Egg Quality ◽  
Sparus Aurata ◽  
Genetic Selection ◽  
Growth Trait ◽  
High Growth ◽  
Gilthead Seabream ◽  
Commercial Diet ◽  
Selection For ◽  
Broodstock Diet

Genetic selection in gilthead seabream (GSB), Sparus aurata, has been undertaken to improve the growth, feed efficiency, fillet quality, skeletal deformities and disease resistance, but no study is available to delineate the effect of genetic selection for growth trait on GSB reproductive performance under mass spawning condition. In this study, high growth (HG) or low growth (LG) GSB broodstock were selected to evaluate the sex steroid hormones, sperm, egg quality and reproductive performance under different feeding regime of commercial diet or experimental broodstock diet containing either fish oil (FO) or vegetable oil (VO) based diet. Under commercial diet feeding phase, broodstock selected for either high growth or low growth did not show any significant changes in the egg production per kg female whereas egg viability percentage was positively (p = 0.014) improved by the high growth trait broodstock group. The experimental diet feeding results revealed that both growth trait and dietary fatty acid composition influenced the reproductive performance of GSB broodstock. In the experimental diet feeding phase, we observed high growth trait GSB males produced a higher number of sperm cells (p < 0.001) and also showed a higher sperm motility (p = 0.048) percentage. The viable egg and larval production per spawn per kg female were significantly improved by the broodstock selected for high growth trait and fed with fish oil-based diet. This present study results signifies that gilthead seabream broodstock selected on growth trait could have positive role in improvement of sperm and egg quality to produce viable progeny.

scholarly journals Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines

2021 ◽  
Vol 22 (13) ◽  
pp. 7141
Author(s):  
Carmen González-Fernández ◽  
Francisco Guillermo Díaz Baños ◽  
María Ángeles Esteban ◽  
Alberto Cuesta
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Fish Cell ◽  
Test Model ◽  
Fish Cell Lines ◽  
Polystyrene Nanoparticles ◽  
Commercial Fish ◽  
Cellular Protection ◽  
Marine Life

Nanoplastics (NPs) are one of the most abundant environment-threatening nanomaterials on the market. The objective of this study was to determine in vitro if functionalized NPs are cytotoxic by themselves or increase the toxicity of metals. For that, we used 50 nm polystyrene nanoparticles with distinct surface functionalization (pristine, PS-Plain; carboxylic, PS-COOH; and amino PS-NH2) alone or combined with the metals arsenic (As) and methylmercury (MeHg), which possess an environmental risk to marine life. As test model, we chose a brain-derived cell line (SaB-1) from gilthead seabream (Sparus aurata), one of the most commercial fish species in the Mediterranean. First, only the PS-NH2 NPs were toxic to SaB-1 cells. NPs seem to be internalized into the cells but they showed little alteration in the transcription of genes related to oxidative stress (nrf2, cat, gr, gsta), cellular protection against metals (mta) or apoptosis (bcl2, bax). However, NPs, mainly PS-COOH and PS-NH2, significantly increased the toxicity of both metals. Since the coexistence of NPs and other pollutants in the aquatic environment is inevitable, our results reveal that the combined effect of NPs with the rest of pollutants deserves more attention.

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