Establishment of an in vitro culture model to study milk production and the blood–milk barrier with bovine mammary epithelial cells

2020 ◽  
Vol 91 (1) ◽  
Author(s):  
Yusaku Tsugami ◽  
Norihiro Suzuki ◽  
Manabu Kawahara ◽  
Takahiro Suzuki ◽  
Takanori Nishimura ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Culture ◽  
Milk Production ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Culture Model

Exogenous phospholipase A2 affects inflammatory gene expression in primary bovine mammary epithelial cells

2019 ◽  
Vol 86 (2) ◽  
pp. 177-180
Author(s):  
Jacqueline P. Kurz ◽  
Mark P. Richards ◽  
Matthew Garcia ◽  
Zhongde Wang
Keyword(s):  
Epithelial Cells ◽  
Phospholipase A2 ◽  
Inflammatory Responses ◽  
Mammary Epithelial Cells ◽  
Constitutive Expression ◽  
Mammary Epithelial ◽  
Inflammatory Factors ◽  
Bovine Mammary Epithelial Cells ◽  
Lps Challenge

AbstractThis Research Communication addresses the hypothesis that exogenously administered phospholipase A2 (PLA2) affects the inflammatory responses of bovine mammary epithelial cells (bMEC) in vitro with the aim of providing preliminary justification of investigation into the uses of exogenously administered PLA2 to manage or treat bovine mastitis. Primary bMEC lines from 11 lactating Holstein dairy cows were established and the expression of 14 pro-inflammatory genes compared under unchallenged and lipopolysaccharide (LPS)-challenged conditions, with and without concurrent treatment with bovine pancreatic PLA2G1B, a secreted form of PLA2. No differences in the expression of these genes were noted between PLA2-treated and untreated bMEC under unchallenged conditions. Following LPS challenge, untreated bMEC exhibited significant downregulation of CXCL8, IL1B, CCL20, and CXCL1. In contrast, PLA2-treated bMEC exhibited significant downregulation of IL1B and CCL20 only. These findings indicate that exogenous PLA2 affects the expression of some pro-inflammatory factors in immune-stimulated bMEC, but does not influence the constitutive expression of these factors. Further investigation of the influence of exogenous PLA2 in the bovine mammary gland is justified.

Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk

2017 ◽  
Vol 53 (8) ◽  
pp. 706-720 ◽  
Author(s):  
Maria Hillreiner ◽  
Nadine I. Müller ◽  
Heiner M. Koch ◽  
Christiane Schmautz ◽  
Bernhard Küster ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
3D Cell Culture ◽  
Mammary Epithelial ◽  
Cell Culture Model ◽  
Bovine Mammary Epithelial Cells ◽  
Fresh Milk ◽  
Culture Model

14-3-3γaffects eIF5 to regulateβ-casein synthesis in bovine mammary epithelial cells

2016 ◽  
Vol 96 (4) ◽  
pp. 478-487
Author(s):  
Cuiping Yu ◽  
Chaochao Luo ◽  
Xinyu Gu ◽  
Yanli Zang ◽  
Bo Qu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Regulatory Mechanism ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Initiation Factor ◽  
Biological Processes ◽  
Bovine Mammary Epithelial Cells ◽  
Protein Levels ◽  
Casein Synthesis

The 14-3-3γ protein participates in many biological processes; however, its regulatory mechanism in milk protein synthesis is not well studied. We hypothesized that 14-3-3γ might affect eIF5 (an initiation factor) to regulate β-casein synthesis in dairy cows. In this study, a possible interaction between 14-3-3γ and eIF5 was investigated using bovine mammary epithelial cells (BMECs). The expression levels of 14-3-3γ and eIF5 in the mammary gland tissues from cows producing higher quality milk were higher than those from cows producing low-quality milk. Moreover, the expression of 14-3-3γ, eIF5, and β-casein were increased at both mRNA and protein levels in BMECs cultured in vitro with methionine (Met) supplementation. Coimmunoprecipitation, colocalization, and FRET analysis further showed the evidences that 14-3-3γ physically bound to eIF5 in BMECs. Gene function studies revealed that 14-3-3γ positively regulated eIF5 through alteration of eIF2α/p-eIF2α ratio. Collectively, our data suggest that 14-3-3γ regulates β-casein translation in BMECs through interaction with eIF5.

scholarly journals Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro

2020 ◽  
Vol 63 ◽  
pp. 104741 ◽  
Author(s):  
Ken Kobayashi ◽  
Yusaku Tsugami ◽  
Norihiro Suzuki ◽  
Takahiro Suzuki ◽  
Takanori Nishimura
Keyword(s):  
Epithelial Cells ◽  
Glucocorticoid Receptor ◽  
Milk Production ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial
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