Short-term evaluation of autologous transplantation of bone marrow-derived mesenchymal stem cells in patients with cirrhosis: Egyptian study

Abstract Background: Bone marrow mesenchymal stem cells (BMSCs) are widely used in many fields such as wound repair, gene delivery, and microenvironment improvement. In some cases, BMSC transplantation requires long-term anesthesia. However, the effects of anesthetics on the characteristics of BMSCs are poorly understood.Methods: In this study, we examined the effect of sevoflurane, a gas anesthetic drug most commonly used in children, on the proliferation, differentiation, and homing potential of BMSCs.Results: Short-term (6 h) sevoflurane exposure had almost no effect on the proliferation, differentiation, and homing of BMSCs. However, long-term (24 h) sevoflurane exposure inhibited the proliferation of BMSCs, accelerated their differentiation into nerve cells, and inhibited their homing potential to damaged vascular endothelial cells and intact glioma cells.Conclusion: Short-term anesthesia with sevoflurane as the main inducer is safe and harmless to BMSCs, but long-term sevoflurane exposure may reduce their repair potential. Therefore, because of the high proportion of BMSCs in children, the application of long-term anesthesia with sevoflurane should be cautious, or more suitable anesthetic drugs are needed.

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Minimally Manipulated Mesenchymal Stem Cells for the Treatment of Knee Osteoarthritis: A Systematic Review of Clinical Evidence

Stem Cells International ◽

10.1155/2019/1735242 ◽

2019 ◽

Vol 2019 ◽

pp. 1-14 ◽

Cited By ~ 16

Author(s):

B. Di Matteo ◽

F. Vandenbulcke ◽

N. D. Vitale ◽

F. Iacono ◽

K. Ashmore ◽

...

Keyword(s):

Systematic Review ◽

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Knee Osteoarthritis ◽

Bone Marrow Aspirate ◽

Stromal Vascular Fraction ◽

Short Term ◽

Keywords Knee ◽

Mesh Terms

Background. The use of laboratory-expanded mesenchymal stem cells (MSCs) is subject to several restrictions, resulting in “minimal manipulation” methods becoming the current most popular strategy to increase the use of MSCs in an orthopaedic practice. The aim of the present systematic review is to assess the clinical applications of “minimally” manipulated MSCs, either as bone marrow aspirate concentrate (BMAC) or as stromal vascular fraction (SVF), in the treatment of knee osteoarthritis (OA). Methods. A systematic review of three databases (PubMed, ScienceDirect, and Google Scholar) was performed using the following keywords: “Knee Osteoarthritis” with “(Bone marrow aspirate) OR (bone marrow concentrate)” or with “(adipose-derived mesenchymal stem cells) OR (adipose derived stromal cells) OR (stromal vascular fraction) OR (SVF)” as either keywords or MeSH terms. The reference lists of all retrieved articles were further reviewed for identification of potentially relevant studies. Results. Twenty-three papers were included in the final analysis (10 on BMAC and 13 on SVF). Of these, only 4 were randomized controlled trials (RCTs). Bias risk evaluation, performed using a modified Coleman score, revealed an overall poor quality of the studies. In terms of clinical application, despite the apparent safety of minimally manipulated MSCs and the short-term positive clinical outcomes associated with their use, clinicians reported different preparation and administration methods, ranging from single intra-articular injections to intraosseous applications to administration in combination with other surgical procedures. Conclusions. The available literature is undermined by both the lack of high-quality studies and the varied clinical settings and different protocols reported in the few RCTs presently published. This prevents any recommendation on the use of either product in a clinical practice. Nevertheless, the use of minimally manipulated MSCs (in the form of BMAC or SVF) has been shown to be safe and have some short-term beneficial effects.

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Short-term evaluation of electromagnetic field pretreatment of adipose-derived stem cells to improve bone healing

Journal of Tissue Engineering and Regenerative Medicine ◽

10.1002/term.1664 ◽

2012 ◽

Vol 9 (10) ◽

pp. 1161-1171 ◽

Cited By ~ 8

Author(s):

Kyung Shin Kang ◽

Jung Min Hong ◽

Young-Joon Seol ◽

Jong-Won Rhie ◽

Young Hun Jeong ◽

...

Keyword(s):

Stem Cells ◽

Electromagnetic Field ◽

Bone Healing ◽

Adipose Derived Stem Cells ◽

Short Term ◽

Short Term Evaluation ◽

Term Evaluation

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Bone Marrow Derived Mesenchymal Stem Cells and Radiation Response: a Kinetic Study of Gene Response Using Microarray Analysis.

Blood ◽

10.1182/blood.v114.22.4575.4575 ◽

2009 ◽

Vol 114 (22) ◽

pp. 4575-4575

Author(s):

Philippe Garrigou ◽

Jean-Francois Mayol ◽

Catherine Mouret ◽

Christophe Delaunay ◽

Michel Drouet ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Target Genes ◽

Conflicts Of Interest ◽

Ex Vivo ◽

Short Term ◽

Hematopoietic Stem ◽

Pai 1

Abstract Abstract 4575 Mesenchymal Stem cells (MSC) are an important radiosensitive component of the so called hematopoietic stem cell niche. Importantly this supportive microenvironment influences the stem cell repopulation capacity as well as the quiescent/non proliferative state of hematopoietic cells. Senescence is considered as a major process in MSC response following irradiation. However, other studies have reported in mice the reduction of the pool of bone marrow mesenchymal stem/progenitor cells following TBI independently of senescence. An altered osteoblastic differentiation was pointed out in these studies. Furthermore, MSC have been shown to be involved in the repair of ionizing radiation damage of distant epithelial sites which requires adherence genes mitigation. The aim of this study was to clarify some of these points using an in vitro model of irradiation and short term culture. Briefly, confluent human BM-MSC were irradiated at the dose of 2.5 Gy (dose rate: 95 cGy.min-1) and immediately put into culture (Minimum essential medium supplemented with 10% FCS and 10 μg/ml of ciprofloxacin, penicillin and streptomycin). Six, 12, 24, 48 and 72 hours after irradiation, cells were harvested and lysed. Total RNAs were purified using the automatic Qiacube system (Qiagen,Courtaboeuf, France) and processed on DNA microarray scanner (Agilent technologies Inc.) according to supplier's recommendations. Data were analyzed with GeneSpring GX Expresion Analysis software version 10.0 (Agilent) in order to identify the transduction pathways involved. No apoptosis was observed during this short term incubation. Among other genes we identified plasminogen activator inhibitor 1 (PAI-1) as a factor highly upregulated after irradiation, in addition to CD151. This is in accordance with MSC response to nutrient-poor, hypoxic stress environment (Copland et al, Stem cells 2009). As MSC are radiosensitive cells, this may indicate that PAI impacts MSC survival through the mitigation of their adhesiveness to surrounding matrices. As PAI-1 is an important factor involved in the balance of blood coagulation and fibrinolysis as well as in the regulation of angiogenesis, one may speculate the consequences of PAI-1 release from MSC on blood homeostasis. Work is going on to describe the main response target genes. This could allow us to identify therapeutic strategies based on ex-vivo or in vivo manipulation of MSC in a purpose of tissue remodelling. Disclosures: No relevant conflicts of interest to declare.

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